Click here to switch to the map view.

The map label for this gene is queF [H]

Identifier: 94309298

GI number: 94309298

Start: 372977

End: 373810

Strand: Reverse

Name: queF [H]

Synonym: Rmet_0353

Alternate gene names: 94309298

Gene position: 373810-372977 (Counterclockwise)

Preceding gene: 94309299

Following gene: 94309296

Centisome position: 9.52

GC content: 63.19

Gene sequence:

>834_bases
ATGACGCTTCCTGAGCACTCGCCGCTGGGCAAGCCCTCGGCGTACAAGACCGAATACGACGCATCATTGCTGTTCCCGAT
CCCGCGCCAGCCGAAACGCGCGGAGATCGGCCTGCCGGAAGGGCGCGCGCTGCCCTTCTTCGGCGTGGATATCTGGAATG
CCTACGAGGTATCGTGGCTGAACCTGAAGGGCAAGCCGCAGGTGGCGCTGGCCACGTTCATCATTCCTGCCGATACGCCG
AACATCGTCGAGTCGAAGTCGTTCAAGCTGTACCTGAACTCGTTCAACCAGACCAAGATCGCTTCGCCCGAGGCGCTGCA
GCAGTTGCTGCATCACGACCTCTCCGAGGCCACGGGCGGCACGGTGCAGGTCCGGCTGGTGACCGAGGCCGACCTTGGCA
CGCAGAAGATGGGCGAACTGGACGGGCTGCTGCTGGACCGGCTCGACATCGAGACCGATATCTACGAACCCGATCCGACG
CTGCTGTCGGCCGAACAGGAAGAGTCGCCGGTGGAGGAAACGCTGGTGTCGCACCTGCTCAAGTCGAACTGCCTGGTCAC
GGGCCAGCCAGACTGGGGTAGCGTGCAGATCCGCTACGTGGGCGCGCCGATCGATCAGGAAGGTCTGCTCAAGTACCTGA
TCTCGTTCCGGAACCATAACGAGTTCCACGAGCAGTGCGTCGAGCGGATCTTCACGGACGTCATGCGCATGTGCAAGCCG
GTGAAGCTCGCGGTCTACGCACGCTACACGCGCCGCGGCGGGCTGGACATCAATCCGTTCCGCACCAACTACAACACGCC
GTGGCCGGACAACCGGCGCAATGCGCGGCAGTAG

Upstream 100 bases:

>100_bases
AGCGCGCGTTCCGCAGCTTCCTTTCGACGCTCGGTTACGTACACTGGGATGAGACCGACAACCCCGTCTACAAGCTGTTC
CTTTCCTGACGCCCCCGATC

Downstream 100 bases:

>100_bases
GACCGCACTGACTCAAAACTCCGCGTGGGCCCGGATGCCGATGAACTTGGCGGGCCCGCGATCCCGGTTGTAGCCCGGGT
TCCGCATGTACTGGAAGTCC

Product: 7-cyano-7-deazaguanine reductase

Products: NA

Alternate protein names: 7-cyano-7-carbaguanine reductase; NADPH-dependent nitrile oxidoreductase; PreQ(0) reductase [H]

Number of amino acids: Translated: 277; Mature: 276

Protein sequence:

>277_residues
MTLPEHSPLGKPSAYKTEYDASLLFPIPRQPKRAEIGLPEGRALPFFGVDIWNAYEVSWLNLKGKPQVALATFIIPADTP
NIVESKSFKLYLNSFNQTKIASPEALQQLLHHDLSEATGGTVQVRLVTEADLGTQKMGELDGLLLDRLDIETDIYEPDPT
LLSAEQEESPVEETLVSHLLKSNCLVTGQPDWGSVQIRYVGAPIDQEGLLKYLISFRNHNEFHEQCVERIFTDVMRMCKP
VKLAVYARYTRRGGLDINPFRTNYNTPWPDNRRNARQ

Sequences:

>Translated_277_residues
MTLPEHSPLGKPSAYKTEYDASLLFPIPRQPKRAEIGLPEGRALPFFGVDIWNAYEVSWLNLKGKPQVALATFIIPADTP
NIVESKSFKLYLNSFNQTKIASPEALQQLLHHDLSEATGGTVQVRLVTEADLGTQKMGELDGLLLDRLDIETDIYEPDPT
LLSAEQEESPVEETLVSHLLKSNCLVTGQPDWGSVQIRYVGAPIDQEGLLKYLISFRNHNEFHEQCVERIFTDVMRMCKP
VKLAVYARYTRRGGLDINPFRTNYNTPWPDNRRNARQ
>Mature_276_residues
TLPEHSPLGKPSAYKTEYDASLLFPIPRQPKRAEIGLPEGRALPFFGVDIWNAYEVSWLNLKGKPQVALATFIIPADTPN
IVESKSFKLYLNSFNQTKIASPEALQQLLHHDLSEATGGTVQVRLVTEADLGTQKMGELDGLLLDRLDIETDIYEPDPTL
LSAEQEESPVEETLVSHLLKSNCLVTGQPDWGSVQIRYVGAPIDQEGLLKYLISFRNHNEFHEQCVERIFTDVMRMCKPV
KLAVYARYTRRGGLDINPFRTNYNTPWPDNRRNARQ

Specific function: Catalyzes the NADPH-dependent reduction of 7-cyano-7- deazaguanine (preQ0) to 7-aminomethyl-7-deazaguanine (preQ1) [H]

COG id: COG0780

COG function: function code R; Enzyme related to GTP cyclohydrolase I

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP cyclohydrolase I family. QueF type 2 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789158, Length=270, Percent_Identity=54.4444444444444, Blast_Score=284, Evalue=4e-78,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016428
- InterPro:   IPR020602 [H]

Pfam domain/function: PF01227 GTP_cyclohydroI [H]

EC number: =1.7.1.13 [H]

Molecular weight: Translated: 31366; Mature: 31235

Theoretical pI: Translated: 5.41; Mature: 5.41

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLPEHSPLGKPSAYKTEYDASLLFPIPRQPKRAEIGLPEGRALPFFGVDIWNAYEVSWL
CCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCEECCEECCCEEEEEE
NLKGKPQVALATFIIPADTPNIVESKSFKLYLNSFNQTKIASPEALQQLLHHDLSEATGG
ECCCCCCEEEEEEEEECCCCCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCC
TVQVRLVTEADLGTQKMGELDGLLLDRLDIETDIYEPDPTLLSAEQEESPVEETLVSHLL
EEEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCEECCCCCCCCHHHHHHHHHH
KSNCLVTGQPDWGSVQIRYVGAPIDQEGLLKYLISFRNHNEFHEQCVERIFTDVMRMCKP
HCCCEEECCCCCCEEEEEEECCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCC
VKLAVYARYTRRGGLDINPFRTNYNTPWPDNRRNARQ
CEEEEEEEHHHCCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TLPEHSPLGKPSAYKTEYDASLLFPIPRQPKRAEIGLPEGRALPFFGVDIWNAYEVSWL
CCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCEECCEECCCEEEEEE
NLKGKPQVALATFIIPADTPNIVESKSFKLYLNSFNQTKIASPEALQQLLHHDLSEATGG
ECCCCCCEEEEEEEEECCCCCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCC
TVQVRLVTEADLGTQKMGELDGLLLDRLDIETDIYEPDPTLLSAEQEESPVEETLVSHLL
EEEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCEECCCCCCCCHHHHHHHHHH
KSNCLVTGQPDWGSVQIRYVGAPIDQEGLLKYLISFRNHNEFHEQCVERIFTDVMRMCKP
HCCCEEECCCCCCEEEEEEECCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCC
VKLAVYARYTRRGGLDINPFRTNYNTPWPDNRRNARQ
CEEEEEEEHHHCCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA