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The map label for this gene is oprM [H]

Identifier: 94309292

GI number: 94309292

Start: 366071

End: 367537

Strand: Reverse

Name: oprM [H]

Synonym: Rmet_0347

Alternate gene names: 94309292

Gene position: 367537-366071 (Counterclockwise)

Preceding gene: 94309293

Following gene: 94309288

Centisome position: 9.36

GC content: 67.21

Gene sequence:

>1467_bases
ATGCGCGCGATCCCCACGCTGTTGATGTGCGCCGCCGCGATGTCGCTGGCCGGCTGTGCGGTCGGCCCGGATTTCCGCAC
GCCCGATGCGCCGGCTGACGCTCGCTACACATCCGGCCCGCAGCCTGAACGCCTTGACGCGAAGGACTACCCATCGCAAT
CGCTGGTGGAAGGCGACGTCCCCGCGCAATGGTGGACGCTGTTCCGCAATCCGCAGCTCGATGAAACCATCCGCGTGGCC
CTGGCCAACAGCCCGACGCTGACACAAGCGCAAGCCAAGCTGCGTGAGGCGCAGGAGAATCTCGCGGCCCGTACCGGCGC
CACGCGCTTCCCGCAGGTAGACGCGAATTTCAACGCAACGCGGCAGCAGGTCAACCTGCAGTCAGTCGGCATCACGCAGT
TCCCGAGCCCTGGGCCGTTCACGCTCTATAACGCGAGTGTGCAGGTGTCCTATGCGCTGGATCTGTTCGGTGGTCAGCGT
CGAGAACTGGAAGGCTTGCAGGCCGCGGTCGACTACCAGCGCTACGAGTTCGAGGCGGCCCGACTTTCGCTCGCGGCCAA
CGTCGCCACGGCGGCGATTCGCGAGGCCGGGCTGCGCGCGCAACTCGCCGATACCGTGGCCCTGGCCGACGCGCAGAAAC
GCCAGCTAGCCATCATGCAGCAGCGCCTCGGTCAGGGTGGTGTCGCGCGCGTCGACGTGCAGCGCCAGCAGGCCGAACTG
GCGCAGACACAGGCGCTGATTCCGGGACTGTCACAGCAGATCGATGCCACACGTCACCAGCTCGCCGTCTACACCGGCCA
GACCCCCGCCGCCGCTCAGCTTCCAGAATTCCGGCTCGAAGACATGCAGTTGCCCGAGACGCTGCCGGTAAGCCTGCCCT
CCGAACTGGCGCGACGTCGCCCCGATATTCGGGCGGCGGAAGCACTGCTCCATCAGGCCTCGGCAGATATCGGCGTGGCC
ACGGCCAATCTCTATCCGCAGATCACGCTGACCGCCAGCGGCGGCACGCAGGCCATGCGCATGAGCGACCTGTTCAACAG
CTTCAATGTGTGGAGCCTGGCCGCCGGCGTCGTGCAGCCGGTGTTCCACGGGGGCGAGTTGCGTGCGCAGAAACGTGCGA
AGGAAGCTGCCTACGAAGCATCGTTGGGCGCCTATCGGCAGGCGGTGCTCCAGGGGCTGCAGAACGTTGCCGACACCCTG
CGCGCGCTGGACGCGGACGCCGGCACGCTGCAGGCGCGCGCGGACAACGCAAAGCAGACGCGCGACACACTGGCGATTGT
CAACGAGCAGTATCGGCTTGGTGGCGTAAGCCAGTTCGCGGTGCTCGACGCCGAGCGGCAGGCACGACAGGCGGCGCTGG
ATCTGGCGCAGGCCCGCGCCACGCGGCTGGCGGATTCAGCCGCGCTGATGCAGGCGCTGGGCGGTGGATGGTGGGGAGAG
TCGGAAACGGTGGGGAGTTTGAAATAG

Upstream 100 bases:

>100_bases
ACTCTGGCCGAACGTGTGGCCCGTGGCGCTGTTCACGGTGATCGTCCTCACCATTGCCGTGCGCTTCTACAAGCGCACGC
TCGACTGAAAGGAGACCGCC

Downstream 100 bases:

>100_bases
AGGTGGTTAGCGATCGTTAGCGGATCGTTAGCGCGGGAGGGGCCGAGCCAGAGGGTCAGGCCCATCAACCGCTACCGCCC
CAGATACCCCCGCAACCGCT

Product: RND efflux system, outer membrane lipoprotein, NodT family

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 488; Mature: 488

Protein sequence:

>488_residues
MRAIPTLLMCAAAMSLAGCAVGPDFRTPDAPADARYTSGPQPERLDAKDYPSQSLVEGDVPAQWWTLFRNPQLDETIRVA
LANSPTLTQAQAKLREAQENLAARTGATRFPQVDANFNATRQQVNLQSVGITQFPSPGPFTLYNASVQVSYALDLFGGQR
RELEGLQAAVDYQRYEFEAARLSLAANVATAAIREAGLRAQLADTVALADAQKRQLAIMQQRLGQGGVARVDVQRQQAEL
AQTQALIPGLSQQIDATRHQLAVYTGQTPAAAQLPEFRLEDMQLPETLPVSLPSELARRRPDIRAAEALLHQASADIGVA
TANLYPQITLTASGGTQAMRMSDLFNSFNVWSLAAGVVQPVFHGGELRAQKRAKEAAYEASLGAYRQAVLQGLQNVADTL
RALDADAGTLQARADNAKQTRDTLAIVNEQYRLGGVSQFAVLDAERQARQAALDLAQARATRLADSAALMQALGGGWWGE
SETVGSLK

Sequences:

>Translated_488_residues
MRAIPTLLMCAAAMSLAGCAVGPDFRTPDAPADARYTSGPQPERLDAKDYPSQSLVEGDVPAQWWTLFRNPQLDETIRVA
LANSPTLTQAQAKLREAQENLAARTGATRFPQVDANFNATRQQVNLQSVGITQFPSPGPFTLYNASVQVSYALDLFGGQR
RELEGLQAAVDYQRYEFEAARLSLAANVATAAIREAGLRAQLADTVALADAQKRQLAIMQQRLGQGGVARVDVQRQQAEL
AQTQALIPGLSQQIDATRHQLAVYTGQTPAAAQLPEFRLEDMQLPETLPVSLPSELARRRPDIRAAEALLHQASADIGVA
TANLYPQITLTASGGTQAMRMSDLFNSFNVWSLAAGVVQPVFHGGELRAQKRAKEAAYEASLGAYRQAVLQGLQNVADTL
RALDADAGTLQARADNAKQTRDTLAIVNEQYRLGGVSQFAVLDAERQARQAALDLAQARATRLADSAALMQALGGGWWGE
SETVGSLK
>Mature_488_residues
MRAIPTLLMCAAAMSLAGCAVGPDFRTPDAPADARYTSGPQPERLDAKDYPSQSLVEGDVPAQWWTLFRNPQLDETIRVA
LANSPTLTQAQAKLREAQENLAARTGATRFPQVDANFNATRQQVNLQSVGITQFPSPGPFTLYNASVQVSYALDLFGGQR
RELEGLQAAVDYQRYEFEAARLSLAANVATAAIREAGLRAQLADTVALADAQKRQLAIMQQRLGQGGVARVDVQRQQAEL
AQTQALIPGLSQQIDATRHQLAVYTGQTPAAAQLPEFRLEDMQLPETLPVSLPSELARRRPDIRAAEALLHQASADIGVA
TANLYPQITLTASGGTQAMRMSDLFNSFNVWSLAAGVVQPVFHGGELRAQKRAKEAAYEASLGAYRQAVLQGLQNVADTL
RALDADAGTLQARADNAKQTRDTLAIVNEQYRLGGVSQFAVLDAERQARQAALDLAQARATRLADSAALMQALGGGWWGE
SETVGSLK

Specific function: The ability to export antibiotics and solvents is dramatically decreased in the presence of the proton conductor carbonyl cyanide m-chlorophenylhydrazone (CCCP), showing that an energized inner membrane is required for efflux. It is thought that the mexB

COG id: COG1538

COG function: function code MU; Outer membrane protein

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor. Note=Attached to the outer membrane by a lipid anchor and via the transmembrane beta-barrel. The membrane anchor is not necessary for antibiotic efflux. In one report (PubMed:10889211) the Cys-18- Gly mutant is reported

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the outer membrane factor (OMF) (TC 1.B.17) family [H]

Homologues:

Organism=Escherichia coli, GI1786785, Length=492, Percent_Identity=27.6422764227642, Blast_Score=119, Evalue=5e-28,
Organism=Escherichia coli, GI2367348, Length=504, Percent_Identity=27.7777777777778, Blast_Score=102, Evalue=7e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003423
- InterPro:   IPR010131 [H]

Pfam domain/function: PF02321 OEP [H]

EC number: NA

Molecular weight: Translated: 52531; Mature: 52531

Theoretical pI: Translated: 5.57; Mature: 5.57

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRAIPTLLMCAAAMSLAGCAVGPDFRTPDAPADARYTSGPQPERLDAKDYPSQSLVEGDV
CCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
PAQWWTLFRNPQLDETIRVALANSPTLTQAQAKLREAQENLAARTGATRFPQVDANFNAT
CHHHHHHHCCCCCCHHHEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHH
RQQVNLQSVGITQFPSPGPFTLYNASVQVSYALDLFGGQRRELEGLQAAVDYQRYEFEAA
HHHEEHHHCCCCCCCCCCCEEEEECEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHH
RLSLAANVATAAIREAGLRAQLADTVALADAQKRQLAIMQQRLGQGGVARVDVQRQQAEL
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHH
AQTQALIPGLSQQIDATRHQLAVYTGQTPAAAQLPEFRLEDMQLPETLPVSLPSELARRR
HHHHHHHCCHHHHHHHHHHEEEEEECCCCCHHCCCCHHHHCCCCCCCCCCCCCHHHHHCC
PDIRAAEALLHQASADIGVATANLYPQITLTASGGTQAMRMSDLFNSFNVWSLAAGVVQP
CCHHHHHHHHHHHCCCCCEEEECCCEEEEEEECCCCHHHHHHHHHCCCCHHHHHHHHHHH
VFHGGELRAQKRAKEAAYEASLGAYRQAVLQGLQNVADTLRALDADAGTLQARADNAKQT
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCHHHH
RDTLAIVNEQYRLGGVSQFAVLDAERQARQAALDLAQARATRLADSAALMQALGGGWWGE
HHHHHHHHHHHHCCCCCHHHEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
SETVGSLK
CCCCCCCC
>Mature Secondary Structure
MRAIPTLLMCAAAMSLAGCAVGPDFRTPDAPADARYTSGPQPERLDAKDYPSQSLVEGDV
CCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
PAQWWTLFRNPQLDETIRVALANSPTLTQAQAKLREAQENLAARTGATRFPQVDANFNAT
CHHHHHHHCCCCCCHHHEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHH
RQQVNLQSVGITQFPSPGPFTLYNASVQVSYALDLFGGQRRELEGLQAAVDYQRYEFEAA
HHHEEHHHCCCCCCCCCCCEEEEECEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHH
RLSLAANVATAAIREAGLRAQLADTVALADAQKRQLAIMQQRLGQGGVARVDVQRQQAEL
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHH
AQTQALIPGLSQQIDATRHQLAVYTGQTPAAAQLPEFRLEDMQLPETLPVSLPSELARRR
HHHHHHHCCHHHHHHHHHHEEEEEECCCCCHHCCCCHHHHCCCCCCCCCCCCCHHHHHCC
PDIRAAEALLHQASADIGVATANLYPQITLTASGGTQAMRMSDLFNSFNVWSLAAGVVQP
CCHHHHHHHHHHHCCCCCEEEECCCEEEEEEECCCCHHHHHHHHHCCCCHHHHHHHHHHH
VFHGGELRAQKRAKEAAYEASLGAYRQAVLQGLQNVADTLRALDADAGTLQARADNAKQT
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCHHHH
RDTLAIVNEQYRLGGVSQFAVLDAERQARQAALDLAQARATRLADSAALMQALGGGWWGE
HHHHHHHHHHHHCCCCCHHHEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
SETVGSLK
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8226684; 10889211; 10984043; 7968531; 8540696; 9401051; 9603892; 10952562 [H]