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The map label for this gene is ptsN [H]

Identifier: 94309245

GI number: 94309245

Start: 317282

End: 317776

Strand: Reverse

Name: ptsN [H]

Synonym: Rmet_0300

Alternate gene names: 94309245

Gene position: 317776-317282 (Counterclockwise)

Preceding gene: 94309246

Following gene: 94309244

Centisome position: 8.09

GC content: 62.02

Gene sequence:

>495_bases
GTGCGTCGGCTCGCCTTTCTGACTCCTCGTGCATCGCCCATGAATCGTTTGGCCAAATTGCTGCCACCCGGCAACATTAA
TCTCGACGTCACCGTCACCAGCAAGAAGCGGGTCTTCGAGCAAGCCGGGCTCCTGTTCGAGAACAACCATGGCGTAGCCC
GGGCCACCGTGACCGACAACCTGTTCGCGCGCGAATCGCTCGGGTCCACCGGTCTTGGCGCCGGCGTCGCCATTCCGCAT
GGCCGCATCAAGGGACTCAAGCAGCCTCTGGCTGCCTTCATGCGCCTGGCCGAGCCAATTCCGTTCGAATCGCCCGACGG
CAAACCGGTGTCGCTGCTGATCTTCCTGCTTGTGCCCGAGCAGGCCACCCAGCAGCATCTGGAGATTCTTTCGGAAATCG
CCCAGTTGTTGTCGGACCGGGAAATGCGTGAAGGACTGGCCACGTTACCCACGCCCGAAGCGGTCCACGAGTTACTGACC
CAGTGGCACCCCTGA

Upstream 100 bases:

>100_bases
AAGTGCGCAGTGGCCCTGCTCTATAATGGCCGGACGCTCCAATGCGGGCGTCCTTTTGCGCTCAATAGCCTGAATTGCCC
AACGCAGCGGGACGACTCAT

Downstream 100 bases:

>100_bases
CACGACGGCAGGCCAGCGCAGCCTGACGCGATACCCGCGATACAGCCCCTCCCGAAAAGTCATCCCGCATGGAACTCACC
GGCGTCACCTCCCAGTCGAT

Product: PTS IIA-like nitrogen-regulatory protein

Products: protein histidine; sugar phosphate

Alternate protein names: Enzyme IIA-NTR; Phosphotransferase enzyme IIA component; PTS system EIIA component [H]

Number of amino acids: Translated: 164; Mature: 164

Protein sequence:

>164_residues
MRRLAFLTPRASPMNRLAKLLPPGNINLDVTVTSKKRVFEQAGLLFENNHGVARATVTDNLFARESLGSTGLGAGVAIPH
GRIKGLKQPLAAFMRLAEPIPFESPDGKPVSLLIFLLVPEQATQQHLEILSEIAQLLSDREMREGLATLPTPEAVHELLT
QWHP

Sequences:

>Translated_164_residues
MRRLAFLTPRASPMNRLAKLLPPGNINLDVTVTSKKRVFEQAGLLFENNHGVARATVTDNLFARESLGSTGLGAGVAIPH
GRIKGLKQPLAAFMRLAEPIPFESPDGKPVSLLIFLLVPEQATQQHLEILSEIAQLLSDREMREGLATLPTPEAVHELLT
QWHP
>Mature_164_residues
MRRLAFLTPRASPMNRLAKLLPPGNINLDVTVTSKKRVFEQAGLLFENNHGVARATVTDNLFARESLGSTGLGAGVAIPH
GRIKGLKQPLAAFMRLAEPIPFESPDGKPVSLLIFLLVPEQATQQHLEILSEIAQLLSDREMREGLATLPTPEAVHELLT
QWHP

Specific function: Seems to have a role in regulating nitrogen assimilation [H]

COG id: COG1762

COG function: function code GT; Phosphotransferase system mannitol/fructose-specific IIA domain (Ntr-type)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIA type-2 domain [H]

Homologues:

Organism=Escherichia coli, GI1789597, Length=132, Percent_Identity=34.8484848484849, Blast_Score=82, Evalue=2e-17,
Organism=Escherichia coli, GI1786951, Length=104, Percent_Identity=35.5769230769231, Blast_Score=68, Evalue=4e-13,
Organism=Escherichia coli, GI2367327, Length=123, Percent_Identity=29.2682926829268, Blast_Score=67, Evalue=4e-13,
Organism=Escherichia coli, GI1788726, Length=153, Percent_Identity=28.7581699346405, Blast_Score=61, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016152
- InterPro:   IPR002178
- InterPro:   IPR006320 [H]

Pfam domain/function: PF00359 PTS_EIIA_2 [H]

EC number: 2.7.1.69

Molecular weight: Translated: 17982; Mature: 17982

Theoretical pI: Translated: 9.28; Mature: 9.28

Prosite motif: PS00372 PTS_EIIA_TYPE_2_HIS ; PS51094 PTS_EIIA_TYPE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRLAFLTPRASPMNRLAKLLPPGNINLDVTVTSKKRVFEQAGLLFENNHGVARATVTDN
CCCCEECCCCCCHHHHHHHHCCCCCCEEEEEECHHHHHHHHHCCEEECCCCEEEEEHHHH
LFARESLGSTGLGAGVAIPHGRIKGLKQPLAAFMRLAEPIPFESPDGKPVSLLIFLLVPE
HHHHHHCCCCCCCCCEECCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHEEEEEEECCC
QATQQHLEILSEIAQLLSDREMREGLATLPTPEAVHELLTQWHP
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCC
>Mature Secondary Structure
MRRLAFLTPRASPMNRLAKLLPPGNINLDVTVTSKKRVFEQAGLLFENNHGVARATVTDN
CCCCEECCCCCCHHHHHHHHCCCCCCEEEEEECHHHHHHHHHCCEEECCCCEEEEEHHHH
LFARESLGSTGLGAGVAIPHGRIKGLKQPLAAFMRLAEPIPFESPDGKPVSLLIFLLVPE
HHHHHHCCCCCCCCCEECCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHEEEEEEECCC
QATQQHLEILSEIAQLLSDREMREGLATLPTPEAVHELLTQWHP
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: protein N(pi)-phosphohistidine; sugar

Specific reaction: protein N(pi)-phosphohistidine + sugar = protein histidine + sugar phosphate

General reaction: Phospho group transfer [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]