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The map label for this gene is ispE

Identifier: 94309235

GI number: 94309235

Start: 305764

End: 306642

Strand: Reverse

Name: ispE

Synonym: Rmet_0290

Alternate gene names: 94309235

Gene position: 306642-305764 (Counterclockwise)

Preceding gene: 94309236

Following gene: 94309234

Centisome position: 7.81

GC content: 62.57

Gene sequence:

>879_bases
ATGACCGTCGGCACGGCCCTGCCGCCACCCGAGCTGCGCAACTGCCCGGCCCCAGCCAAGCTCAACCTGTTCCTGCACGT
GACCGGCCGCCGCCCCGACGGCTACCACACGTTGCAGACCGTGTTCCAGCTCATCGACTGGTCTGACACGCTGCACTTCC
ATCGCCGTGACGATGGCGTGATCACGCGTGTTACCGACATCCCCGGGGTGCCGGCCGATACCGACCTCGTCGTGCGCGCG
GCCCGCGCAATGCAGGCGGCCAGCGGCACGCGTTTCGGCGTGGACATCGCCATCGACAAGATCCTGCCCATGGGTGGCGG
CATCGGCGGCGGGTCGTCCGACGCCGCCACCACGCTGCTGGCGCTGAACCGCCTGTGGGGCGTGAACCTGCCGCGCGAAG
AACTGATGCGTATCGGCCTTTCGCTCGGCGCGGACGTGCCCGTATTCGTGTTCGGCCAGAACGCGTTCGCCGAGGGCGTG
GGCGAGGAATTGACGCCCGTGGCGCTGCCGGACAGCTGGTTCGTGGTCATCCATCCACGGCAACATGTGCCGACCGCAGC
AATATTTTCGGACGAACGCTTGACAAGGAATTCGCCAATCTCTATAGTTGCGGACTTCGCTGCTTGCACAAACAAATTCG
CTTTCGGTCGCAACGATCTGGAAACGATAGCAACAGCGAAGTTCGGCGAAGTTGCCCGAGCCCTTGAGTGGCTGAAAAAT
TACAGTCCACATGCAAGGATGACCGGTTCCGGAGCCTGCGTATTTGCCCGCTTTGAAGACGAGCAGACTGCGCAGAGAGT
GATGGAAAGGTTGCCATCCGAGTGGGATGGCAGGTGTGTGAAAAGTTTGTCACATCACCCGCTCGCAACGTTCGCGTAG

Upstream 100 bases:

>100_bases
CCACGCCCACGACCACGCTGCCCCGCCGTATCGACATGGCACGCGATGGCAATGAATCGCCGCTGTCGGTGCGGCTCGTA
ATCGACCCGGAGAACAAAGA

Downstream 100 bases:

>100_bases
CAGGTTTTCGCCTGGCGATGTACTGACGGTTTAAAGTCAGCGCAACGACAGACGTTAAAGGTTGTGTAGGGGAGTCGCCA
AGTTGGTCAAGGCACCGGAT

Product: 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase

Products: NA

Alternate protein names: CMK; 4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase

Number of amino acids: Translated: 292; Mature: 291

Protein sequence:

>292_residues
MTVGTALPPPELRNCPAPAKLNLFLHVTGRRPDGYHTLQTVFQLIDWSDTLHFHRRDDGVITRVTDIPGVPADTDLVVRA
ARAMQAASGTRFGVDIAIDKILPMGGGIGGGSSDAATTLLALNRLWGVNLPREELMRIGLSLGADVPVFVFGQNAFAEGV
GEELTPVALPDSWFVVIHPRQHVPTAAIFSDERLTRNSPISIVADFAACTNKFAFGRNDLETIATAKFGEVARALEWLKN
YSPHARMTGSGACVFARFEDEQTAQRVMERLPSEWDGRCVKSLSHHPLATFA

Sequences:

>Translated_292_residues
MTVGTALPPPELRNCPAPAKLNLFLHVTGRRPDGYHTLQTVFQLIDWSDTLHFHRRDDGVITRVTDIPGVPADTDLVVRA
ARAMQAASGTRFGVDIAIDKILPMGGGIGGGSSDAATTLLALNRLWGVNLPREELMRIGLSLGADVPVFVFGQNAFAEGV
GEELTPVALPDSWFVVIHPRQHVPTAAIFSDERLTRNSPISIVADFAACTNKFAFGRNDLETIATAKFGEVARALEWLKN
YSPHARMTGSGACVFARFEDEQTAQRVMERLPSEWDGRCVKSLSHHPLATFA
>Mature_291_residues
TVGTALPPPELRNCPAPAKLNLFLHVTGRRPDGYHTLQTVFQLIDWSDTLHFHRRDDGVITRVTDIPGVPADTDLVVRAA
RAMQAASGTRFGVDIAIDKILPMGGGIGGGSSDAATTLLALNRLWGVNLPREELMRIGLSLGADVPVFVFGQNAFAEGVG
EELTPVALPDSWFVVIHPRQHVPTAAIFSDERLTRNSPISIVADFAACTNKFAFGRNDLETIATAKFGEVARALEWLKNY
SPHARMTGSGACVFARFEDEQTAQRVMERLPSEWDGRCVKSLSHHPLATFA

Specific function: Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol

COG id: COG1947

COG function: function code I; 4-diphosphocytidyl-2C-methyl-D-erythritol 2-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GHMP kinase family. IspE subfamily

Homologues:

Organism=Escherichia coli, GI1787459, Length=279, Percent_Identity=50.8960573476703, Blast_Score=248, Evalue=3e-67,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPE_RALME (Q1LRQ0)

Other databases:

- EMBL:   CP000352
- RefSeq:   YP_582445.1
- ProteinModelPortal:   Q1LRQ0
- SMR:   Q1LRQ0
- STRING:   Q1LRQ0
- GeneID:   4037077
- GenomeReviews:   CP000352_GR
- KEGG:   rme:Rmet_0290
- eggNOG:   COG1947
- HOGENOM:   HBG734593
- OMA:   LNLFLHV
- PhylomeDB:   Q1LRQ0
- ProtClustDB:   PRK00343
- BioCyc:   RMET266264:RMET_0290-MONOMER
- HAMAP:   MF_00061
- InterPro:   IPR006204
- InterPro:   IPR013750
- InterPro:   IPR004424
- InterPro:   IPR020568
- InterPro:   IPR014721
- Gene3D:   G3DSA:3.30.230.10
- PANTHER:   PTHR20861:SF2
- PIRSF:   PIRSF010376
- TIGRFAMs:   TIGR00154

Pfam domain/function: PF08544 GHMP_kinases_C; PF00288 GHMP_kinases_N; SSF54211 Ribosomal_S5_D2-typ_fold

EC number: =2.7.1.148

Molecular weight: Translated: 31782; Mature: 31650

Theoretical pI: Translated: 6.60; Mature: 6.60

Prosite motif: NA

Important sites: ACT_SITE 20-20 ACT_SITE 145-145

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVGTALPPPELRNCPAPAKLNLFLHVTGRRPDGYHTLQTVFQLIDWSDTLHFHRRDDGV
CCCCCCCCCHHHHCCCCCCEEEEEEEEECCCCCCHHHHHHHHHHHCCCCCEEEEECCCCE
ITRVTDIPGVPADTDLVVRAARAMQAASGTRFGVDIAIDKILPMGGGIGGGSSDAATTLL
EEEEECCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEEEEHHCCCCCCCCCCCHHHHHHHH
ALNRLWGVNLPREELMRIGLSLGADVPVFVFGQNAFAEGVGEELTPVALPDSWFVVIHPR
HHHHHHCCCCCHHHHHHHHHHCCCCCCEEEECCCHHHHCCCCCCCCEECCCCEEEEECCC
QHVPTAAIFSDERLTRNSPISIVADFAACTNKFAFGRNDLETIATAKFGEVARALEWLKN
CCCCCCEEECCCCCCCCCCHHEEEHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHC
YSPHARMTGSGACVFARFEDEQTAQRVMERLPSEWDGRCVKSLSHHPLATFA
CCCCCEECCCCCEEEEEECCHHHHHHHHHHCCCCCCCHHHHHCCCCCCCCCC
>Mature Secondary Structure 
TVGTALPPPELRNCPAPAKLNLFLHVTGRRPDGYHTLQTVFQLIDWSDTLHFHRRDDGV
CCCCCCCCHHHHCCCCCCEEEEEEEEECCCCCCHHHHHHHHHHHCCCCCEEEEECCCCE
ITRVTDIPGVPADTDLVVRAARAMQAASGTRFGVDIAIDKILPMGGGIGGGSSDAATTLL
EEEEECCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEEEEHHCCCCCCCCCCCHHHHHHHH
ALNRLWGVNLPREELMRIGLSLGADVPVFVFGQNAFAEGVGEELTPVALPDSWFVVIHPR
HHHHHHCCCCCHHHHHHHHHHCCCCCCEEEECCCHHHHCCCCCCCCEECCCCEEEEECCC
QHVPTAAIFSDERLTRNSPISIVADFAACTNKFAFGRNDLETIATAKFGEVARALEWLKN
CCCCCCEEECCCCCCCCCCHHEEEHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHC
YSPHARMTGSGACVFARFEDEQTAQRVMERLPSEWDGRCVKSLSHHPLATFA
CCCCCEECCCCCEEEEEECCHHHHHHHHHHCCCCCCCHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA