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The map label for this gene is prs [H]

Identifier: 94309234

GI number: 94309234

Start: 304571

End: 305524

Strand: Reverse

Name: prs [H]

Synonym: Rmet_0289

Alternate gene names: 94309234

Gene position: 305524-304571 (Counterclockwise)

Preceding gene: 94309235

Following gene: 94309233

Centisome position: 7.78

GC content: 63.73

Gene sequence:

>954_bases
ATGAGCGTCGAAGGCTTGATGGTATTTACCGGCAATGCCAACCCTAAACTCGCGGAGGCTGTCGTACAGCACCTCGGCAT
TCCGCTCGGCAAGGCTCTGGTCGGCCGTTTCTCCGACGGTGAAGTCCAGGTAGAAATCCAGGAAAACGTTCGCGGCAAGC
ACGTCATCGTGCTGCAGTCGACCTGCGCGCCGACCAACGACAACCTGATGGAACTGATGGTGATGGTCGACGCGCTCAAG
CGCGCTTCGGCACGCAGCATCACCGCAGCCATGCCCTACTTCGGCTATGCCCGCCAAGATCGCCGCCCGCGTTCGGCGCG
CGTGGCCATCTCGGCCAAGATCGTGGCCAACATGCTGGAAGTGGCCGGCGTGGAACGCGTGCTGACGATGGACCTGCACG
CTGACCAGATCCAGGGCTTCTTCGATATCCCCGTCGACAACATCTACGCATCCCCGGTCCTGCTTGGCGATCTGCGCGAG
AAGAACTACGGCAATCTGCTGGTAGTGTCTCCGGACGTCGGCGGCGTGGTGCGCGCCCGTGCGCTGGCCAAGCAGCTCGA
CTGCGACCTGGCCATCATCGACAAGCGTCGCCCGAAGGCCAACGTGTCCGAGGTGATGAACATCATCGGTGAAGTCGCCG
GCCGCAACTGCGTGATCATGGACGACATGATCGACACCGGCGGCACGCTGTGCAAGGCTGCGCAGGTGCTGAAGGAGCGT
GGCGCCCAGAAGGTATTCGCGTACTGCACGCACCCGGTGCTGTCGGGTGGCGCGGTTGCCCGCATCGCCGCGTCCGCGCT
GGACGAAGTGGTGGTGTGCGACACGATTCCGCTGTCTGACGAAGCCCGTCAGACCGGCAAGATCCGTCAGGTCACGACCG
CCCCGCTGCTGGCCGAGACCTTCACCCGGATCGTCCGGGGCGATTCGATCATGTCGCTGTTCGCCGACGCCTGA

Upstream 100 bases:

>100_bases
TGCCATTACTTCCTATAGTGTCCAGAGCGGACATGCAGAAATTCGCTGCCGGCATTGCCGGTGGCGCAAAGCCCCCCCAG
TAGCAAGACAGGTGCCCCGA

Downstream 100 bases:

>100_bases
TCGGCACTGATCGGGACGGTACTGGCGGATACCTGCTAGAATATTTGGCTTTACTGCGTTGGCCGCCTGAATGGGCGGCC
AACGTGATTCAGGCGGCGCA

Product: ribose-phosphate pyrophosphokinase

Products: NA

Alternate protein names: RPPK; Phosphoribosyl pyrophosphate synthase; P-Rib-PP synthase; PRPP synthase [H]

Number of amino acids: Translated: 317; Mature: 316

Protein sequence:

>317_residues
MSVEGLMVFTGNANPKLAEAVVQHLGIPLGKALVGRFSDGEVQVEIQENVRGKHVIVLQSTCAPTNDNLMELMVMVDALK
RASARSITAAMPYFGYARQDRRPRSARVAISAKIVANMLEVAGVERVLTMDLHADQIQGFFDIPVDNIYASPVLLGDLRE
KNYGNLLVVSPDVGGVVRARALAKQLDCDLAIIDKRRPKANVSEVMNIIGEVAGRNCVIMDDMIDTGGTLCKAAQVLKER
GAQKVFAYCTHPVLSGGAVARIAASALDEVVVCDTIPLSDEARQTGKIRQVTTAPLLAETFTRIVRGDSIMSLFADA

Sequences:

>Translated_317_residues
MSVEGLMVFTGNANPKLAEAVVQHLGIPLGKALVGRFSDGEVQVEIQENVRGKHVIVLQSTCAPTNDNLMELMVMVDALK
RASARSITAAMPYFGYARQDRRPRSARVAISAKIVANMLEVAGVERVLTMDLHADQIQGFFDIPVDNIYASPVLLGDLRE
KNYGNLLVVSPDVGGVVRARALAKQLDCDLAIIDKRRPKANVSEVMNIIGEVAGRNCVIMDDMIDTGGTLCKAAQVLKER
GAQKVFAYCTHPVLSGGAVARIAASALDEVVVCDTIPLSDEARQTGKIRQVTTAPLLAETFTRIVRGDSIMSLFADA
>Mature_316_residues
SVEGLMVFTGNANPKLAEAVVQHLGIPLGKALVGRFSDGEVQVEIQENVRGKHVIVLQSTCAPTNDNLMELMVMVDALKR
ASARSITAAMPYFGYARQDRRPRSARVAISAKIVANMLEVAGVERVLTMDLHADQIQGFFDIPVDNIYASPVLLGDLREK
NYGNLLVVSPDVGGVVRARALAKQLDCDLAIIDKRRPKANVSEVMNIIGEVAGRNCVIMDDMIDTGGTLCKAAQVLKERG
AQKVFAYCTHPVLSGGAVARIAASALDEVVVCDTIPLSDEARQTGKIRQVTTAPLLAETFTRIVRGDSIMSLFADA

Specific function: Utilized by both the de novo and the salvage pathways by which endogenously formed or exogenously added pyrimidine, purine, or pyridine bases are converted to the corresponding ribonucleoside monophosphates. [C]

COG id: COG0462

COG function: function code FE; Phosphoribosylpyrophosphate synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ribose-phosphate pyrophosphokinase family [H]

Homologues:

Organism=Homo sapiens, GI4506129, Length=313, Percent_Identity=46.0063897763578, Blast_Score=289, Evalue=2e-78,
Organism=Homo sapiens, GI84875539, Length=315, Percent_Identity=45.3968253968254, Blast_Score=288, Evalue=6e-78,
Organism=Homo sapiens, GI4506127, Length=313, Percent_Identity=45.6869009584665, Blast_Score=286, Evalue=1e-77,
Organism=Homo sapiens, GI28557709, Length=313, Percent_Identity=44.7284345047923, Blast_Score=283, Evalue=1e-76,
Organism=Homo sapiens, GI4506133, Length=348, Percent_Identity=36.4942528735632, Blast_Score=201, Evalue=8e-52,
Organism=Homo sapiens, GI194018537, Length=345, Percent_Identity=36.231884057971, Blast_Score=196, Evalue=2e-50,
Organism=Homo sapiens, GI310128524, Length=149, Percent_Identity=37.5838926174497, Blast_Score=98, Evalue=1e-20,
Organism=Homo sapiens, GI310115209, Length=149, Percent_Identity=37.5838926174497, Blast_Score=98, Evalue=1e-20,
Organism=Homo sapiens, GI310118259, Length=149, Percent_Identity=37.5838926174497, Blast_Score=98, Evalue=1e-20,
Organism=Homo sapiens, GI310119946, Length=149, Percent_Identity=37.5838926174497, Blast_Score=98, Evalue=1e-20,
Organism=Escherichia coli, GI1787458, Length=310, Percent_Identity=64.5161290322581, Blast_Score=415, Evalue=1e-117,
Organism=Caenorhabditis elegans, GI25149168, Length=313, Percent_Identity=44.7284345047923, Blast_Score=285, Evalue=3e-77,
Organism=Caenorhabditis elegans, GI17554704, Length=310, Percent_Identity=45.1612903225806, Blast_Score=285, Evalue=3e-77,
Organism=Caenorhabditis elegans, GI17554702, Length=313, Percent_Identity=44.7284345047923, Blast_Score=285, Evalue=3e-77,
Organism=Caenorhabditis elegans, GI71989924, Length=317, Percent_Identity=44.1640378548896, Blast_Score=283, Evalue=6e-77,
Organism=Caenorhabditis elegans, GI17570245, Length=341, Percent_Identity=34.8973607038123, Blast_Score=209, Evalue=2e-54,
Organism=Saccharomyces cerevisiae, GI6320946, Length=320, Percent_Identity=43.4375, Blast_Score=266, Evalue=3e-72,
Organism=Saccharomyces cerevisiae, GI6319403, Length=321, Percent_Identity=42.3676012461059, Blast_Score=259, Evalue=3e-70,
Organism=Saccharomyces cerevisiae, GI6321776, Length=318, Percent_Identity=43.0817610062893, Blast_Score=254, Evalue=1e-68,
Organism=Saccharomyces cerevisiae, GI6322667, Length=196, Percent_Identity=43.3673469387755, Blast_Score=158, Evalue=1e-39,
Organism=Saccharomyces cerevisiae, GI6324511, Length=109, Percent_Identity=39.4495412844037, Blast_Score=96, Evalue=6e-21,
Organism=Drosophila melanogaster, GI21355239, Length=314, Percent_Identity=45.859872611465, Blast_Score=278, Evalue=3e-75,
Organism=Drosophila melanogaster, GI45551540, Length=336, Percent_Identity=42.8571428571429, Blast_Score=269, Evalue=2e-72,
Organism=Drosophila melanogaster, GI24651458, Length=358, Percent_Identity=33.2402234636872, Blast_Score=199, Evalue=3e-51,
Organism=Drosophila melanogaster, GI24651456, Length=358, Percent_Identity=33.2402234636872, Blast_Score=199, Evalue=3e-51,
Organism=Drosophila melanogaster, GI281362873, Length=358, Percent_Identity=33.2402234636872, Blast_Score=198, Evalue=4e-51,
Organism=Drosophila melanogaster, GI24651454, Length=358, Percent_Identity=33.2402234636872, Blast_Score=198, Evalue=4e-51,
Organism=Drosophila melanogaster, GI24651462, Length=206, Percent_Identity=37.8640776699029, Blast_Score=145, Evalue=3e-35,
Organism=Drosophila melanogaster, GI24651464, Length=206, Percent_Identity=37.8640776699029, Blast_Score=145, Evalue=3e-35,
Organism=Drosophila melanogaster, GI45552010, Length=206, Percent_Identity=37.8640776699029, Blast_Score=145, Evalue=3e-35,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000842
- InterPro:   IPR005946
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00156 Pribosyltran [H]

EC number: =2.7.6.1 [H]

Molecular weight: Translated: 34116; Mature: 33985

Theoretical pI: Translated: 6.91; Mature: 6.91

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER ; PS00114 PRPP_SYNTHETASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVEGLMVFTGNANPKLAEAVVQHLGIPLGKALVGRFSDGEVQVEIQENVRGKHVIVLQS
CCCCEEEEEECCCCHHHHHHHHHHCCCCCCHHHHCCCCCCEEEEEECCCCCCCEEEEEEC
TCAPTNDNLMELMVMVDALKRASARSITAAMPYFGYARQDRRPRSARVAISAKIVANMLE
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCCCCHHEEEEHHHHHHHHHH
VAGVERVLTMDLHADQIQGFFDIPVDNIYASPVLLGDLREKNYGNLLVVSPDVGGVVRAR
HHCHHHEEEECCCHHHHCCEEECCHHHHCCCCEEEECCCCCCCCCEEEECCCCCHHHHHH
ALAKQLDCDLAIIDKRRPKANVSEVMNIIGEVAGRNCVIMDDMIDTGGTLCKAAQVLKER
HHHHHHCCCEEEEECCCCCCCHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHHHC
GAQKVFAYCTHPVLSGGAVARIAASALDEVVVCDTIPLSDEARQTGKIRQVTTAPLLAET
CHHHHHHHHCCHHHCCCHHHHHHHHHHCCEEEECCCCCCHHHHHCCCEEEEHHHHHHHHH
FTRIVRGDSIMSLFADA
HHHHHCCCHHHHHHCCC
>Mature Secondary Structure 
SVEGLMVFTGNANPKLAEAVVQHLGIPLGKALVGRFSDGEVQVEIQENVRGKHVIVLQS
CCCEEEEEECCCCHHHHHHHHHHCCCCCCHHHHCCCCCCEEEEEECCCCCCCEEEEEEC
TCAPTNDNLMELMVMVDALKRASARSITAAMPYFGYARQDRRPRSARVAISAKIVANMLE
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCCCCHHEEEEHHHHHHHHHH
VAGVERVLTMDLHADQIQGFFDIPVDNIYASPVLLGDLREKNYGNLLVVSPDVGGVVRAR
HHCHHHEEEECCCHHHHCCEEECCHHHHCCCCEEEECCCCCCCCCEEEECCCCCHHHHHH
ALAKQLDCDLAIIDKRRPKANVSEVMNIIGEVAGRNCVIMDDMIDTGGTLCKAAQVLKER
HHHHHHCCCEEEEECCCCCCCHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHHHC
GAQKVFAYCTHPVLSGGAVARIAASALDEVVVCDTIPLSDEARQTGKIRQVTTAPLLAET
CHHHHHHHHCCHHHCCCHHHHHHHHHHCCEEEECCCCCCHHHHHCCCEEEEHHHHHHHHH
FTRIVRGDSIMSLFADA
HHHHHCCCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11823852 [H]