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The map label for this gene is maiA [H]

Identifier: 94309225

GI number: 94309225

Start: 296200

End: 296847

Strand: Direct

Name: maiA [H]

Synonym: Rmet_0280

Alternate gene names: 94309225

Gene position: 296200-296847 (Clockwise)

Preceding gene: 94309224

Following gene: 335055485

Centisome position: 7.54

GC content: 60.96

Gene sequence:

>648_bases
ATGCTCGAACTCCATAGCTACTTCCGCAGCTCGGCGTCGTTCAGGGTGCGTATCGCGCTGAACCTGAAGGGCTTGCCCTA
TGACTACAAGGGTGTGCATCTGCTGAAGGAAGGCGGCATGCAGCTGAAGCCCGAATATCGCGCGCTGAATGCCGATGGCG
TGGTGCCGACGCTCGTGGTCGATGGCAGGCCGCTGATTCAGTCGATGGCGATCATCGAGTATCTCGACGAAACCCATCCG
GAGCCTGCGCTGCTGCCGAAGGATCCGTTCGAGCGTGCCGTCGTGCGCGGCCTGGCTCAGGAAATCGCCTGCGAGATCCA
TCCGCTCAACAACCTGCGCGTGCTCAAGTACCTCAAGCACGAGCTCAAGGTGTCGGAGGAAGCCAAGGATGCGTGGTACC
GCCACTGGATCGAACAGGGGTTTGCCTCGCTCGAAGTCAATCTGTCGCAACTGGGGCGTGTGGGTCGCTTTGCGTTCGGC
GATACGCCGACATTGATCGACTGCTGCCTGGTGCCGCAGGTATTCAATGCGCAGCGCTTCAATGTTGATGTGAGCCGCTT
CCCGACGATCGCGAAGATCCATGCCACTTGCATGGAGCTGGACGCGTTCCAGAAGGCCGCGCCCGGGGCGCAGCCGGACA
GCGAGTAA

Upstream 100 bases:

>100_bases
AGCGGAACGCCTGAAGGCGTGGGGCCGGTGGTGAAGGGCGACGTCATGCAATGCCACATCGAAGGTGTGGGCGACCTGAC
CGTCAAGGTGGTGTGAGGCG

Downstream 100 bases:

>100_bases
GCGGTAGCGTTGCCAGAAACAAAAACCCCGGTGTCCTCACCGGGGTTTTTGTTTTTGCGTGCCAGCCGGACTCAGCCCAG
CAGCGCCTCGGCGAACTCGT

Product: maleylacetoacetate isomerase

Products: NA

Alternate protein names: MAAI [H]

Number of amino acids: Translated: 215; Mature: 215

Protein sequence:

>215_residues
MLELHSYFRSSASFRVRIALNLKGLPYDYKGVHLLKEGGMQLKPEYRALNADGVVPTLVVDGRPLIQSMAIIEYLDETHP
EPALLPKDPFERAVVRGLAQEIACEIHPLNNLRVLKYLKHELKVSEEAKDAWYRHWIEQGFASLEVNLSQLGRVGRFAFG
DTPTLIDCCLVPQVFNAQRFNVDVSRFPTIAKIHATCMELDAFQKAAPGAQPDSE

Sequences:

>Translated_215_residues
MLELHSYFRSSASFRVRIALNLKGLPYDYKGVHLLKEGGMQLKPEYRALNADGVVPTLVVDGRPLIQSMAIIEYLDETHP
EPALLPKDPFERAVVRGLAQEIACEIHPLNNLRVLKYLKHELKVSEEAKDAWYRHWIEQGFASLEVNLSQLGRVGRFAFG
DTPTLIDCCLVPQVFNAQRFNVDVSRFPTIAKIHATCMELDAFQKAAPGAQPDSE
>Mature_215_residues
MLELHSYFRSSASFRVRIALNLKGLPYDYKGVHLLKEGGMQLKPEYRALNADGVVPTLVVDGRPLIQSMAIIEYLDETHP
EPALLPKDPFERAVVRGLAQEIACEIHPLNNLRVLKYLKHELKVSEEAKDAWYRHWIEQGFASLEVNLSQLGRVGRFAFG
DTPTLIDCCLVPQVFNAQRFNVDVSRFPTIAKIHATCMELDAFQKAAPGAQPDSE

Specific function: Forms An Equimolar Complex With The RNA Polymerase Holoenzyme (Rnap) But Not With The Core Enzyme. It Is Synthesized Predominantly When Cells Are Exposed To Amino Acid Starvation, At Which Time It Accounts For Over 50% Of The Total Protein Synthesized. It

COG id: COG0625

COG function: function code O; Glutathione S-transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GST N-terminal domain [H]

Homologues:

Organism=Homo sapiens, GI22202624, Length=211, Percent_Identity=47.8672985781991, Blast_Score=186, Evalue=2e-47,
Organism=Homo sapiens, GI22202622, Length=160, Percent_Identity=46.875, Blast_Score=134, Evalue=5e-32,
Organism=Homo sapiens, GI194440732, Length=211, Percent_Identity=36.9668246445498, Blast_Score=123, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI17551302, Length=211, Percent_Identity=42.654028436019, Blast_Score=167, Evalue=4e-42,
Organism=Caenorhabditis elegans, GI17510461, Length=211, Percent_Identity=37.914691943128, Blast_Score=141, Evalue=2e-34,
Organism=Caenorhabditis elegans, GI17556142, Length=201, Percent_Identity=39.8009950248756, Blast_Score=129, Evalue=2e-30,
Organism=Drosophila melanogaster, GI21355859, Length=210, Percent_Identity=47.6190476190476, Blast_Score=187, Evalue=3e-48,
Organism=Drosophila melanogaster, GI24645375, Length=210, Percent_Identity=47.6190476190476, Blast_Score=187, Evalue=3e-48,
Organism=Drosophila melanogaster, GI45553325, Length=210, Percent_Identity=47.6190476190476, Blast_Score=187, Evalue=3e-48,
Organism=Drosophila melanogaster, GI21355857, Length=211, Percent_Identity=43.1279620853081, Blast_Score=173, Evalue=6e-44,

Paralogues:

None

Copy number: 480 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1982 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010987
- InterPro:   IPR004045
- InterPro:   IPR017933
- InterPro:   IPR004046
- InterPro:   IPR005955
- InterPro:   IPR012336
- InterPro:   IPR012335 [H]

Pfam domain/function: PF00043 GST_C; PF02798 GST_N [H]

EC number: =5.2.1.2 [H]

Molecular weight: Translated: 24229; Mature: 24229

Theoretical pI: Translated: 6.62; Mature: 6.62

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLELHSYFRSSASFRVRIALNLKGLPYDYKGVHLLKEGGMQLKPEYRALNADGVVPTLVV
CCCHHHHHCCCCCEEEEEEEEECCCCCCCCCCCEEECCCCEECCHHHCCCCCCCCEEEEE
DGRPLIQSMAIIEYLDETHPEPALLPKDPFERAVVRGLAQEIACEIHPLNNLRVLKYLKH
CCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHH
ELKVSEEAKDAWYRHWIEQGFASLEVNLSQLGRVGRFAFGDTPTLIDCCLVPQVFNAQRF
HHHCCHHHHHHHHHHHHHHCHHEEEECHHHHCCHHCCCCCCCCHHHHHHHHHHHHCCCCC
NVDVSRFPTIAKIHATCMELDAFQKAAPGAQPDSE
CCCHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MLELHSYFRSSASFRVRIALNLKGLPYDYKGVHLLKEGGMQLKPEYRALNADGVVPTLVV
CCCHHHHHCCCCCEEEEEEEEECCCCCCCCCCCEEECCCCEECCHHHCCCCCCCCEEEEE
DGRPLIQSMAIIEYLDETHPEPALLPKDPFERAVVRGLAQEIACEIHPLNNLRVLKYLKH
CCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHH
ELKVSEEAKDAWYRHWIEQGFASLEVNLSQLGRVGRFAFGDTPTLIDCCLVPQVFNAQRF
HHHCCHHHHHHHHHHHHHHCHHEEEECHHHHCCHHCCCCCCCCHHHHHHHHHHHHCCCCC
NVDVSRFPTIAKIHATCMELDAFQKAAPGAQPDSE
CCCHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]