Click here to switch to the map view.

The map label for this gene is leuD [H]

Identifier: 94309146

GI number: 94309146

Start: 209944

End: 210597

Strand: Direct

Name: leuD [H]

Synonym: Rmet_0201

Alternate gene names: 94309146

Gene position: 209944-210597 (Clockwise)

Preceding gene: 94309145

Following gene: 94309147

Centisome position: 5.34

GC content: 65.75

Gene sequence:

>654_bases
ATGGATGACATCACGACGATCGAAGGTGTCGGCGCGCCACTACCGCTGCCGAACCTGGATACCGACCAGATCATGCCGAA
GCAGTTCCTGCGTGGCATCGACAAGGCGGGGCTCGACCGCGGGCTGCTTCACGACATGCGTTTCGACGCGAACGGAGCCC
CGCGTGCGGACTTCGTGCTGAACCAGCCGGCGTGGCGCGACACGCGCGTGCTGGTCGCCGGGCCGAACTTCGGCTGCGGT
TCGAGCCGTGAGCACGCGGTCTGGGGGCTGCAGCAATATGGCATCCGCGCGGTCATCGCGCCGAGCTTCGGCGAGATCTT
CTACTCGAACGCGATGAACAACCGGTTGTTGCTGGTGGCGCTGCCGCAGGCGGATGTCGACCAGATCATGGCGGCCGTGA
GTGACCCCGTGGCGCCGGCGTCGATCCGTATCGACCTGTCGACCATGACGGTCAATGCCGGCGACTTCGCCAAGTCGTTC
TCGCTCTCTCCGCGCCACCTCGACATGTTCCGGCTCGGCCTCGATGTCATCGGCATGTCGTTGACGCACGCCGATGCGAT
TGCGGCATTCACAAGTCGGCACCACGCGGACAACCCCTGGCTACGCGACGTGGCCGCGACTACCATGGCACGGCTCGGCC
GCATGGCTGGCTGA

Upstream 100 bases:

>100_bases
CAGGGCCGTGCCGGACGCACGCACCTGATGAGCCCGGCCATGGCAGCCGCCGCAGCCGTTACCGGCTGTATCACCGACGT
GCGGGAACTGGAGATCCAAC

Downstream 100 bases:

>100_bases
CGGAGGAGATACGATGTTCGAAGGATTCACGACCCAGCGCATTGATCACGACGGCGTGGCGATCCATGCGGTGGTCGGCG
GAGAAGGTCCGCCGCTGCTG

Product: 3-isopropylmalate isomerase subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase 1; IPMI 1; Isopropylmalate isomerase 1 [H]

Number of amino acids: Translated: 217; Mature: 217

Protein sequence:

>217_residues
MDDITTIEGVGAPLPLPNLDTDQIMPKQFLRGIDKAGLDRGLLHDMRFDANGAPRADFVLNQPAWRDTRVLVAGPNFGCG
SSREHAVWGLQQYGIRAVIAPSFGEIFYSNAMNNRLLLVALPQADVDQIMAAVSDPVAPASIRIDLSTMTVNAGDFAKSF
SLSPRHLDMFRLGLDVIGMSLTHADAIAAFTSRHHADNPWLRDVAATTMARLGRMAG

Sequences:

>Translated_217_residues
MDDITTIEGVGAPLPLPNLDTDQIMPKQFLRGIDKAGLDRGLLHDMRFDANGAPRADFVLNQPAWRDTRVLVAGPNFGCG
SSREHAVWGLQQYGIRAVIAPSFGEIFYSNAMNNRLLLVALPQADVDQIMAAVSDPVAPASIRIDLSTMTVNAGDFAKSF
SLSPRHLDMFRLGLDVIGMSLTHADAIAAFTSRHHADNPWLRDVAATTMARLGRMAG
>Mature_217_residues
MDDITTIEGVGAPLPLPNLDTDQIMPKQFLRGIDKAGLDRGLLHDMRFDANGAPRADFVLNQPAWRDTRVLVAGPNFGCG
SSREHAVWGLQQYGIRAVIAPSFGEIFYSNAMNNRLLLVALPQADVDQIMAAVSDPVAPASIRIDLSTMTVNAGDFAKSF
SLSPRHLDMFRLGLDVIGMSLTHADAIAAFTSRHHADNPWLRDVAATTMARLGRMAG

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate [H]

COG id: COG0066

COG function: function code E; 3-isopropylmalate dehydratase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leuD family. LeuD type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786258, Length=191, Percent_Identity=44.5026178010471, Blast_Score=154, Evalue=4e-39,
Organism=Saccharomyces cerevisiae, GI6321429, Length=201, Percent_Identity=39.8009950248756, Blast_Score=142, Evalue=6e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004431
- InterPro:   IPR015937
- InterPro:   IPR015928
- InterPro:   IPR000573
- InterPro:   IPR015936 [H]

Pfam domain/function: PF00694 Aconitase_C [H]

EC number: =4.2.1.33 [H]

Molecular weight: Translated: 23524; Mature: 23524

Theoretical pI: Translated: 6.23; Mature: 6.23

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
4.6 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDDITTIEGVGAPLPLPNLDTDQIMPKQFLRGIDKAGLDRGLLHDMRFDANGAPRADFVL
CCCCCCCCCCCCCCCCCCCCHHHCCHHHHHHHHHHCCCCHHHHHHHCCCCCCCCCCCEEE
NQPAWRDTRVLVAGPNFGCGSSREHAVWGLQQYGIRAVIAPSFGEIFYSNAMNNRLLLVA
CCCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHCEEEEECCCHHHHHHHHCCCCEEEEEE
LPQADVDQIMAAVSDPVAPASIRIDLSTMTVNAGDFAKSFSLSPRHLDMFRLGLDVIGMS
CCCCCHHHHHHHHCCCCCCCEEEEEEEEEEECCCHHHHHCCCCCCHHHHHHHHHHHHHHH
LTHADAIAAFTSRHHADNPWLRDVAATTMARLGRMAG
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MDDITTIEGVGAPLPLPNLDTDQIMPKQFLRGIDKAGLDRGLLHDMRFDANGAPRADFVL
CCCCCCCCCCCCCCCCCCCCHHHCCHHHHHHHHHHCCCCHHHHHHHCCCCCCCCCCCEEE
NQPAWRDTRVLVAGPNFGCGSSREHAVWGLQQYGIRAVIAPSFGEIFYSNAMNNRLLLVA
CCCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHCEEEEECCCHHHHHHHHCCCCEEEEEE
LPQADVDQIMAAVSDPVAPASIRIDLSTMTVNAGDFAKSFSLSPRHLDMFRLGLDVIGMS
CCCCCHHHHHHHHCCCCCCCEEEEEEEEEEECCCHHHHHCCCCCCHHHHHHHHHHHHHHH
LTHADAIAAFTSRHHADNPWLRDVAATTMARLGRMAG
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 14500782 [H]