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The map label for this gene is leuC [H]

Identifier: 94309145

GI number: 94309145

Start: 208470

End: 209951

Strand: Direct

Name: leuC [H]

Synonym: Rmet_0200

Alternate gene names: 94309145

Gene position: 208470-209951 (Clockwise)

Preceding gene: 94309142

Following gene: 94309146

Centisome position: 5.31

GC content: 66.8

Gene sequence:

>1482_bases
ATGGATTCCGCGATTAGACTGCGTGCATTCATTCATTCGAATTGCATTCACCCAGCCAATGCCATGACCGGTCGCACGCT
ATATCAGAAGCTCGTGGACTCGCACACGGTCGTCACGCTCGATGCGGACAACGTGCTGCTGTACGCGGATCTCCACATCA
TGAACGAGTACACGAGTCCGCAGGCGTTCGCCGGGCTCGCCGAGCAGGGCCGCAAGGTGGCGGCGCCGGGCCAGAACCTG
GCCGTGGTCGATCACATCATCCCGACGCACGCGGAATCCCCGCGCGTGATCCAGGATCCCGCCTCGGCGCTGCAGGCTCG
CAACCTGAAACGTAATTGCGACCTGCACGGTATTCCGCTGTTCGACACCAATGATCCGCTGCAGGGTATCGAGCATGTCA
TCGCGCCCGAGCACGGCATGATCCGGCCCGGCATGGTCGTGCTTTGCGGCGACAGCCATACCACCACCTACGGCGCATTC
GGCGCGCTGGGCTTCGGTATCGGCACCTCCGAGGTGGAGCACGTGCTGGCCACGCAGACGCTGGTCTACCGGCTGGCGCG
CGACATGCGTATCCGCGTGGATGGCCCGCTGCCATCGGGCACGACTTCGAAAGACCTGATCCTCCACATCATCGCGCGCA
TCAGCGCGCAGGGCGCGCGTGGCTACGCGGTGGAGTTCACGGGCAGCACGATCCGTGCGTTGTCGATGGAAGCGCGCATG
ACCCTGTGCAACATGACCGTGGAGGCGGGCGCGCGCGGGGCACTGATCGCGCCCGATGCCACGTCGATCGACTATGTCCT
GCGCCGGGCACCAGACATCGACGCCGACATGGCCGAAACCGCGCGCCGGGCATGGGCCGGGCTGCGGTCGGATGCCGATG
CCGGCTTCGATCTCGAACTGGCATTCGATGCCGCTGCGGTGGAGCCGTTCGTCACCTGGGGCACGAGCCCGGATCAGGCG
ATTCCCGTGAGCGCCACGGTGCCGCAGCCGGCCGACGCCGGGGACAGCAACGCCCGCGTTTCCGCCGAGCGCGCGCTTGA
GTACATCGGCGTGACGCCCGGCGTGTCGCTGCAGGGGCTGCCTGTGCAGCGGGTGTTTATCGGCTCCTGCACGAATGGCC
GCATCGAGGACTTGCGCCAGGTGGCCGCCGTGGTACGCGGCCGCAGGGTGGCGCCGGGCGTACGTGCAATGGTGGTGCCG
GGCTCCGGCGCCGTGCGCGATCAGGCTGAAGCCGAGGGCATCGCGGCCTTGCTGATCGATGCTGGATTCGAATGGCGCAA
GCCCGGCTGTTCGATGTGCCTTGCGATGAACGACGACGTGTTGGCGCCGGGCGAGCGCTGCGCGTCGACCACCAACCGAA
ACTTCGAGGGCCGCCAGGGCCGTGCCGGACGCACGCACCTGATGAGCCCGGCCATGGCAGCCGCCGCAGCCGTTACCGGC
TGTATCACCGACGTGCGGGAACTGGAGATCCAACATGGATGA

Upstream 100 bases:

>100_bases
AAGAACGCGAGATCGGAGAAGGCATCCATGGCGGCCGATTATTCGTGATGCATTCGTGAATTAAAGGCACGAATGTAAGT
AATTTGGCGAAATTATAGCG

Downstream 100 bases:

>100_bases
CATCACGACGATCGAAGGTGTCGGCGCGCCACTACCGCTGCCGAACCTGGATACCGACCAGATCATGCCGAAGCAGTTCC
TGCGTGGCATCGACAAGGCG

Product: isopropylmalate isomerase large subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase 1; IPMI 1; Isopropylmalate isomerase 1 [H]

Number of amino acids: Translated: 493; Mature: 493

Protein sequence:

>493_residues
MDSAIRLRAFIHSNCIHPANAMTGRTLYQKLVDSHTVVTLDADNVLLYADLHIMNEYTSPQAFAGLAEQGRKVAAPGQNL
AVVDHIIPTHAESPRVIQDPASALQARNLKRNCDLHGIPLFDTNDPLQGIEHVIAPEHGMIRPGMVVLCGDSHTTTYGAF
GALGFGIGTSEVEHVLATQTLVYRLARDMRIRVDGPLPSGTTSKDLILHIIARISAQGARGYAVEFTGSTIRALSMEARM
TLCNMTVEAGARGALIAPDATSIDYVLRRAPDIDADMAETARRAWAGLRSDADAGFDLELAFDAAAVEPFVTWGTSPDQA
IPVSATVPQPADAGDSNARVSAERALEYIGVTPGVSLQGLPVQRVFIGSCTNGRIEDLRQVAAVVRGRRVAPGVRAMVVP
GSGAVRDQAEAEGIAALLIDAGFEWRKPGCSMCLAMNDDVLAPGERCASTTNRNFEGRQGRAGRTHLMSPAMAAAAAVTG
CITDVRELEIQHG

Sequences:

>Translated_493_residues
MDSAIRLRAFIHSNCIHPANAMTGRTLYQKLVDSHTVVTLDADNVLLYADLHIMNEYTSPQAFAGLAEQGRKVAAPGQNL
AVVDHIIPTHAESPRVIQDPASALQARNLKRNCDLHGIPLFDTNDPLQGIEHVIAPEHGMIRPGMVVLCGDSHTTTYGAF
GALGFGIGTSEVEHVLATQTLVYRLARDMRIRVDGPLPSGTTSKDLILHIIARISAQGARGYAVEFTGSTIRALSMEARM
TLCNMTVEAGARGALIAPDATSIDYVLRRAPDIDADMAETARRAWAGLRSDADAGFDLELAFDAAAVEPFVTWGTSPDQA
IPVSATVPQPADAGDSNARVSAERALEYIGVTPGVSLQGLPVQRVFIGSCTNGRIEDLRQVAAVVRGRRVAPGVRAMVVP
GSGAVRDQAEAEGIAALLIDAGFEWRKPGCSMCLAMNDDVLAPGERCASTTNRNFEGRQGRAGRTHLMSPAMAAAAAVTG
CITDVRELEIQHG
>Mature_493_residues
MDSAIRLRAFIHSNCIHPANAMTGRTLYQKLVDSHTVVTLDADNVLLYADLHIMNEYTSPQAFAGLAEQGRKVAAPGQNL
AVVDHIIPTHAESPRVIQDPASALQARNLKRNCDLHGIPLFDTNDPLQGIEHVIAPEHGMIRPGMVVLCGDSHTTTYGAF
GALGFGIGTSEVEHVLATQTLVYRLARDMRIRVDGPLPSGTTSKDLILHIIARISAQGARGYAVEFTGSTIRALSMEARM
TLCNMTVEAGARGALIAPDATSIDYVLRRAPDIDADMAETARRAWAGLRSDADAGFDLELAFDAAAVEPFVTWGTSPDQA
IPVSATVPQPADAGDSNARVSAERALEYIGVTPGVSLQGLPVQRVFIGSCTNGRIEDLRQVAAVVRGRRVAPGVRAMVVP
GSGAVRDQAEAEGIAALLIDAGFEWRKPGCSMCLAMNDDVLAPGERCASTTNRNFEGRQGRAGRTHLMSPAMAAAAAVTG
CITDVRELEIQHG

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate [H]

COG id: COG0065

COG function: function code E; 3-isopropylmalate dehydratase large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aconitase/IPM isomerase family. LeuC type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI41352693, Length=387, Percent_Identity=26.3565891472868, Blast_Score=97, Evalue=4e-20,
Organism=Homo sapiens, GI8659555, Length=386, Percent_Identity=27.4611398963731, Blast_Score=97, Evalue=4e-20,
Organism=Homo sapiens, GI4501867, Length=368, Percent_Identity=26.6304347826087, Blast_Score=96, Evalue=8e-20,
Organism=Escherichia coli, GI1786259, Length=465, Percent_Identity=53.5483870967742, Blast_Score=499, Evalue=1e-142,
Organism=Escherichia coli, GI1787531, Length=355, Percent_Identity=27.0422535211268, Blast_Score=84, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17568399, Length=404, Percent_Identity=25.7425742574257, Blast_Score=100, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI32564738, Length=364, Percent_Identity=26.0989010989011, Blast_Score=99, Evalue=5e-21,
Organism=Caenorhabditis elegans, GI25149337, Length=364, Percent_Identity=26.0989010989011, Blast_Score=99, Evalue=5e-21,
Organism=Caenorhabditis elegans, GI25149342, Length=299, Percent_Identity=25.4180602006689, Blast_Score=89, Evalue=4e-18,
Organism=Saccharomyces cerevisiae, GI6321429, Length=473, Percent_Identity=52.0084566596194, Blast_Score=477, Evalue=1e-135,
Organism=Saccharomyces cerevisiae, GI6320440, Length=477, Percent_Identity=27.0440251572327, Blast_Score=158, Evalue=2e-39,
Organism=Saccharomyces cerevisiae, GI6322261, Length=368, Percent_Identity=27.1739130434783, Blast_Score=119, Evalue=1e-27,
Organism=Saccharomyces cerevisiae, GI6323335, Length=364, Percent_Identity=26.6483516483516, Blast_Score=115, Evalue=1e-26,
Organism=Drosophila melanogaster, GI281365315, Length=481, Percent_Identity=24.7401247401247, Blast_Score=96, Evalue=7e-20,
Organism=Drosophila melanogaster, GI17864292, Length=481, Percent_Identity=24.7401247401247, Blast_Score=96, Evalue=7e-20,
Organism=Drosophila melanogaster, GI161076999, Length=448, Percent_Identity=25.2232142857143, Blast_Score=95, Evalue=1e-19,
Organism=Drosophila melanogaster, GI28571643, Length=363, Percent_Identity=26.4462809917355, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24645686, Length=371, Percent_Identity=27.2237196765499, Blast_Score=83, Evalue=5e-16,
Organism=Drosophila melanogaster, GI17137564, Length=380, Percent_Identity=26.0526315789474, Blast_Score=75, Evalue=8e-14,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004430
- InterPro:   IPR015931
- InterPro:   IPR015937
- InterPro:   IPR001030
- InterPro:   IPR015932
- InterPro:   IPR018136
- InterPro:   IPR015936 [H]

Pfam domain/function: PF00330 Aconitase [H]

EC number: =4.2.1.33 [H]

Molecular weight: Translated: 52423; Mature: 52423

Theoretical pI: Translated: 6.02; Mature: 6.02

Prosite motif: PS00450 ACONITASE_1 ; PS01244 ACONITASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDSAIRLRAFIHSNCIHPANAMTGRTLYQKLVDSHTVVTLDADNVLLYADLHIMNEYTSP
CCCHHEEHHHHHCCCCCCCCHHHHHHHHHHHHCCCEEEEEECCCEEEEEEEEEECCCCCH
QAFAGLAEQGRKVAAPGQNLAVVDHIIPTHAESPRVIQDPASALQARNLKRNCDLHGIPL
HHHHHHHHCCCEECCCCCCEEEEEHHCCCCCCCCCEECCHHHHHHHHCHHHCCCCCCCEE
FDTNDPLQGIEHVIAPEHGMIRPGMVVLCGDSHTTTYGAFGALGFGIGTSEVEHVLATQT
ECCCCHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCHHCHHHHHCCCCHHHHHHHHHHHH
LVYRLARDMRIRVDGPLPSGTTSKDLILHIIARISAQGARGYAVEFTGSTIRALSMEARM
HHHHHHHCCEEEECCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEECCCEEEEHHHHHHH
TLCNMTVEAGARGALIAPDATSIDYVLRRAPDIDADMAETARRAWAGLRSDADAGFDLEL
HHHHEEHHCCCCCEEECCCCCHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCEEEEE
AFDAAAVEPFVTWGTSPDQAIPVSATVPQPADAGDSNARVSAERALEYIGVTPGVSLQGL
EECCHHCCCCEECCCCCCCEEEEECCCCCCCCCCCCCCEEEHHHHHHHHCCCCCCCCCCC
PVQRVFIGSCTNGRIEDLRQVAAVVRGRRVAPGVRAMVVPGSGAVRDQAEAEGIAALLID
CEEEEEEECCCCCHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCHHHHCCEEEEEEE
AGFEWRKPGCSMCLAMNDDVLAPGERCASTTNRNFEGRQGRAGRTHLMSPAMAAAAAVTG
CCCCCCCCCCCEEEEECCCCCCCCHHHHHCCCCCCCCCCCCCCCHHHCCHHHHHHHHHHH
CITDVRELEIQHG
HHHHHHHHEECCC
>Mature Secondary Structure
MDSAIRLRAFIHSNCIHPANAMTGRTLYQKLVDSHTVVTLDADNVLLYADLHIMNEYTSP
CCCHHEEHHHHHCCCCCCCCHHHHHHHHHHHHCCCEEEEEECCCEEEEEEEEEECCCCCH
QAFAGLAEQGRKVAAPGQNLAVVDHIIPTHAESPRVIQDPASALQARNLKRNCDLHGIPL
HHHHHHHHCCCEECCCCCCEEEEEHHCCCCCCCCCEECCHHHHHHHHCHHHCCCCCCCEE
FDTNDPLQGIEHVIAPEHGMIRPGMVVLCGDSHTTTYGAFGALGFGIGTSEVEHVLATQT
ECCCCHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCHHCHHHHHCCCCHHHHHHHHHHHH
LVYRLARDMRIRVDGPLPSGTTSKDLILHIIARISAQGARGYAVEFTGSTIRALSMEARM
HHHHHHHCCEEEECCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEECCCEEEEHHHHHHH
TLCNMTVEAGARGALIAPDATSIDYVLRRAPDIDADMAETARRAWAGLRSDADAGFDLEL
HHHHEEHHCCCCCEEECCCCCHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCEEEEE
AFDAAAVEPFVTWGTSPDQAIPVSATVPQPADAGDSNARVSAERALEYIGVTPGVSLQGL
EECCHHCCCCEECCCCCCCEEEEECCCCCCCCCCCCCCEEEHHHHHHHHCCCCCCCCCCC
PVQRVFIGSCTNGRIEDLRQVAAVVRGRRVAPGVRAMVVPGSGAVRDQAEAEGIAALLID
CEEEEEEECCCCCHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCHHHHCCEEEEEEE
AGFEWRKPGCSMCLAMNDDVLAPGERCASTTNRNFEGRQGRAGRTHLMSPAMAAAAAVTG
CCCCCCCCCCCEEEEECCCCCCCCHHHHHCCCCCCCCCCCCCCCHHHCCHHHHHHHHHHH
CITDVRELEIQHG
HHHHHHHHEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA