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The map label for this gene is glmU

Identifier: 94309131

GI number: 94309131

Start: 192473

End: 193837

Strand: Direct

Name: glmU

Synonym: Rmet_0186

Alternate gene names: 94309131

Gene position: 192473-193837 (Clockwise)

Preceding gene: 94309129

Following gene: 94309132

Centisome position: 4.9

GC content: 66.23

Gene sequence:

>1365_bases
GTGAATATCGTCATTCTTGCCGCGGGCATGGGCAAGCGGATGAACTCCGCTCTGCCCAAGGTGCTGCACCCGGTGGCCGG
CCAGCCAATGCTATCCCACGTCCTCGATACCGCGCGCACGCTGTCGCCGTCGCGGCTCGTGGTGGTGGTGGGTCATGGTG
CCGAACTGGTGCGCAAAGCCGTTGGCGCGGACGATGTTGCCTTTGCCGAGCAGGCTCAGCAACTGGGCACCGGCCACGCC
GTGATGCAGGCCCTGCCGCTGCTCGACGACAGCCAGCCCACGCTGGTGCTGTACGGCGACGTGCCGCTGACCTCGGTCGA
CACCCTGAAGGGCCTCGTGCAGGCCGCGGGCGCCGAGCGTCTGGGCGTGCTGACTGTCGAGATGCCCGATCCTACCGGAT
ATGGACGGATCGTTCGTGACGCCGCCGGCAATATCGTGCGCATCGTCGAGCAGAAGGACGCCAGCGAGGACGTCCGCGCG
ATTCGCGAGATCAACACCGGCATTATCGTCTGCCCGACGGCGCATCTGCGCCAGTGGCTGGCCACGCTGCGCAACGACAA
CTCGCAGGGCGAGTACTACCTGACCGATACGATCGAGCGCGCCGTCAATGATGGCGTGGAGGTCGTGTCGGCCCAGCCGG
CCGCGCTGTGGGAGACGCTCGGCGTCAACAGCAAGGTCCAGCTTGCCGAGATCGAACGTATTCACCAGCGCAACATTGCC
CAGCGGTTGCTGGAGGCGGGCGTCACGCTGCTCGACCCGGCACGCATCGACGTGCGCGGCGAGCTAACCTGCGGGCGCGA
CGTCACCATCGACGTGGGCTGCGTGTTCGAGGGCCGCGTGCACCTGGAAGACGGCGTGTCCGTTGGCGCGCACTGCGTCG
TTCGCAACACGACGATCGGCGCCGGCGCGCGTATCCAGCCGTTCTGCCACTTCGAGGACGCCAAGGTCGGGCCTGACGGA
CGCATCGGACCGTACGCGCGGTTGCGCCCGGGCACGGAACTCGGCCAGGACGTGCATATCGGCAATTTCGTCGAGATCAA
GAACAGCCAGATCGCCGACCACAGCAAGGCCAATCACCTGGCCTACGTGGGCGACGCGACGGTGGGGCAGCGCGTCAATA
TTGGCGCGGGTACCATTACCTGCAATTACGATGGCGTGAACAAGCACCGCACGGTGCTCGAGGACGATGTGTTCATCGGC
TCCGACACCCAGCTGGTGGCGCCGGTGACGGTGCGCCGCGGCGCCACGATTGGCGCGGGGACCACGCTGACCAAGGAAGC
GCCGGCTGACAAGCTGACGTTGTCGCGCGCCAAGCAGATGACCCTGGATGCGTGGCAGCGTCCGGTCAAGCAACCGAAGA
AATAA

Upstream 100 bases:

>100_bases
TTTGGGCGCGGCCAGCCAGTTCGGTGCACGGCCGGACGCGGCGCTCCCAGGCACGGATTCTCCAGCTTATTCTCAGACAC
ACTCACTTAGGGACCTCCTT

Downstream 100 bases:

>100_bases
GAGGGGTTCAAAACATGTGTGGCATCGTTGGGGCGGTCTCTCACCGCAATATCGTTCCTGTCCTGATCGAAGGCCTGCGC
CGCCTTGAGTACCGTGGCTA

Product: multifunctional N-acetyl glucosamine-1-phosphate uridyltransferase/glucosamine-1-phosphate acetyl transferase

Products: NA

Alternate protein names: UDP-N-acetylglucosamine pyrophosphorylase; N-acetylglucosamine-1-phosphate uridyltransferase; Glucosamine-1-phosphate N-acetyltransferase

Number of amino acids: Translated: 454; Mature: 454

Protein sequence:

>454_residues
MNIVILAAGMGKRMNSALPKVLHPVAGQPMLSHVLDTARTLSPSRLVVVVGHGAELVRKAVGADDVAFAEQAQQLGTGHA
VMQALPLLDDSQPTLVLYGDVPLTSVDTLKGLVQAAGAERLGVLTVEMPDPTGYGRIVRDAAGNIVRIVEQKDASEDVRA
IREINTGIIVCPTAHLRQWLATLRNDNSQGEYYLTDTIERAVNDGVEVVSAQPAALWETLGVNSKVQLAEIERIHQRNIA
QRLLEAGVTLLDPARIDVRGELTCGRDVTIDVGCVFEGRVHLEDGVSVGAHCVVRNTTIGAGARIQPFCHFEDAKVGPDG
RIGPYARLRPGTELGQDVHIGNFVEIKNSQIADHSKANHLAYVGDATVGQRVNIGAGTITCNYDGVNKHRTVLEDDVFIG
SDTQLVAPVTVRRGATIGAGTTLTKEAPADKLTLSRAKQMTLDAWQRPVKQPKK

Sequences:

>Translated_454_residues
MNIVILAAGMGKRMNSALPKVLHPVAGQPMLSHVLDTARTLSPSRLVVVVGHGAELVRKAVGADDVAFAEQAQQLGTGHA
VMQALPLLDDSQPTLVLYGDVPLTSVDTLKGLVQAAGAERLGVLTVEMPDPTGYGRIVRDAAGNIVRIVEQKDASEDVRA
IREINTGIIVCPTAHLRQWLATLRNDNSQGEYYLTDTIERAVNDGVEVVSAQPAALWETLGVNSKVQLAEIERIHQRNIA
QRLLEAGVTLLDPARIDVRGELTCGRDVTIDVGCVFEGRVHLEDGVSVGAHCVVRNTTIGAGARIQPFCHFEDAKVGPDG
RIGPYARLRPGTELGQDVHIGNFVEIKNSQIADHSKANHLAYVGDATVGQRVNIGAGTITCNYDGVNKHRTVLEDDVFIG
SDTQLVAPVTVRRGATIGAGTTLTKEAPADKLTLSRAKQMTLDAWQRPVKQPKK
>Mature_454_residues
MNIVILAAGMGKRMNSALPKVLHPVAGQPMLSHVLDTARTLSPSRLVVVVGHGAELVRKAVGADDVAFAEQAQQLGTGHA
VMQALPLLDDSQPTLVLYGDVPLTSVDTLKGLVQAAGAERLGVLTVEMPDPTGYGRIVRDAAGNIVRIVEQKDASEDVRA
IREINTGIIVCPTAHLRQWLATLRNDNSQGEYYLTDTIERAVNDGVEVVSAQPAALWETLGVNSKVQLAEIERIHQRNIA
QRLLEAGVTLLDPARIDVRGELTCGRDVTIDVGCVFEGRVHLEDGVSVGAHCVVRNTTIGAGARIQPFCHFEDAKVGPDG
RIGPYARLRPGTELGQDVHIGNFVEIKNSQIADHSKANHLAYVGDATVGQRVNIGAGTITCNYDGVNKHRTVLEDDVFIG
SDTQLVAPVTVRRGATIGAGTTLTKEAPADKLTLSRAKQMTLDAWQRPVKQPKK

Specific function: Catalyzes the last two sequential reactions in the de novo biosynthetic pathway for UDP-GlcNAc. Responsible for the acetylation of Glc-N-1-P to give GlcNAc-1-P and for the uridyl transfer from UTP to GlcNAc-1-P which produces UDP-GlcNAc

COG id: COG1207

COG function: function code M; N-acetylglucosamine-1-phosphate uridyltransferase (contains nucleotidyltransferase and I-patch acetyltransferase domains)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the transferase hexapeptide repeat family

Homologues:

Organism=Escherichia coli, GI1790168, Length=451, Percent_Identity=51.8847006651885, Blast_Score=474, Evalue=1e-135,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GLMU_RALME (Q1LS04)

Other databases:

- EMBL:   CP000352
- RefSeq:   YP_582341.1
- ProteinModelPortal:   Q1LS04
- SMR:   Q1LS04
- STRING:   Q1LS04
- GeneID:   4036971
- GenomeReviews:   CP000352_GR
- KEGG:   rme:Rmet_0186
- eggNOG:   COG1207
- HOGENOM:   HBG688195
- OMA:   GSKVNHL
- PhylomeDB:   Q1LS04
- ProtClustDB:   CLSK896503
- BioCyc:   RMET266264:RMET_0186-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01631
- InterPro:   IPR005882
- InterPro:   IPR005835
- InterPro:   IPR011004
- TIGRFAMs:   TIGR01173

Pfam domain/function: PF00483 NTP_transferase; SSF51161 Trimer_LpxA_like

EC number: =2.7.7.23; =2.3.1.157

Molecular weight: Translated: 48654; Mature: 48654

Theoretical pI: Translated: 6.70; Mature: 6.70

Prosite motif: PS00101 HEXAPEP_TRANSFERASES

Important sites: ACT_SITE 359-359 BINDING 71-71 BINDING 135-135 BINDING 150-150 BINDING 165-165 BINDING 383-383 BINDING 401-401 BINDING 419-419 BINDING 436-436

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIVILAAGMGKRMNSALPKVLHPVAGQPMLSHVLDTARTLSPSRLVVVVGHGAELVRKA
CEEEEEECCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCEEEEEEECCHHHHHHH
VGADDVAFAEQAQQLGTGHAVMQALPLLDDSQPTLVLYGDVPLTSVDTLKGLVQAAGAER
HCCCHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCEEEECCCCCCHHHHHHHHHHHCCCCC
LGVLTVEMPDPTGYGRIVRDAAGNIVRIVEQKDASEDVRAIREINTGIIVCPTAHLRQWL
EEEEEEECCCCCCCCHHHHHCCCCEEEEEECCCCHHHHHHHHHCCCCEEEECHHHHHHHH
ATLRNDNSQGEYYLTDTIERAVNDGVEVVSAQPAALWETLGVNSKVQLAEIERIHQRNIA
HHHHCCCCCCCEEEHHHHHHHHCCCCEEEECCCHHHHHHHCCCCCCHHHHHHHHHHHHHH
QRLLEAGVTLLDPARIDVRGELTCGRDVTIDVGCVFEGRVHLEDGVSVGAHCVVRNTTIG
HHHHHCCCEEECCCEEEEEEEEECCCCCEEEEEEEEECEEEECCCCCCCCEEEEEECCCC
AGARIQPFCHFEDAKVGPDGRIGPYARLRPGTELGQDVHIGNFVEIKNSQIADHSKANHL
CCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCE
AYVGDATVGQRVNIGAGTITCNYDGVNKHRTVLEDDVFIGSDTQLVAPVTVRRGATIGAG
EEECCCCCCCEEECCCEEEEEECCCCCCCCEEEECCEEECCCCEEEEEEEEECCCEECCC
TTLTKEAPADKLTLSRAKQMTLDAWQRPVKQPKK
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MNIVILAAGMGKRMNSALPKVLHPVAGQPMLSHVLDTARTLSPSRLVVVVGHGAELVRKA
CEEEEEECCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCEEEEEEECCHHHHHHH
VGADDVAFAEQAQQLGTGHAVMQALPLLDDSQPTLVLYGDVPLTSVDTLKGLVQAAGAER
HCCCHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCEEEECCCCCCHHHHHHHHHHHCCCCC
LGVLTVEMPDPTGYGRIVRDAAGNIVRIVEQKDASEDVRAIREINTGIIVCPTAHLRQWL
EEEEEEECCCCCCCCHHHHHCCCCEEEEEECCCCHHHHHHHHHCCCCEEEECHHHHHHHH
ATLRNDNSQGEYYLTDTIERAVNDGVEVVSAQPAALWETLGVNSKVQLAEIERIHQRNIA
HHHHCCCCCCCEEEHHHHHHHHCCCCEEEECCCHHHHHHHCCCCCCHHHHHHHHHHHHHH
QRLLEAGVTLLDPARIDVRGELTCGRDVTIDVGCVFEGRVHLEDGVSVGAHCVVRNTTIG
HHHHHCCCEEECCCEEEEEEEEECCCCCEEEEEEEEECEEEECCCCCCCCEEEEEECCCC
AGARIQPFCHFEDAKVGPDGRIGPYARLRPGTELGQDVHIGNFVEIKNSQIADHSKANHL
CCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCE
AYVGDATVGQRVNIGAGTITCNYDGVNKHRTVLEDDVFIGSDTQLVAPVTVRRGATIGAG
EEECCCCCCCEEECCCEEEEEECCCCCCCCEEEECCEEECCCCEEEEEEEEECCCEECCC
TTLTKEAPADKLTLSRAKQMTLDAWQRPVKQPKK
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA