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The map label for this gene is ttcA

Identifier: 94309129

GI number: 94309129

Start: 190405

End: 191349

Strand: Direct

Name: ttcA

Synonym: Rmet_0184

Alternate gene names: 94309129

Gene position: 190405-191349 (Clockwise)

Preceding gene: 94309128

Following gene: 94309131

Centisome position: 4.85

GC content: 62.22

Gene sequence:

>945_bases
ATGAGTCACTCCGCTAACTTCTATCGTCTCGAAACCCGTCTGCAGTCGCTGACCGGCAAGGCCATCGGCGACTTCGGCAT
GATCGAAGACGGCGACACAGTGCTGGTCTGCATGAGTGGCGGCAAGGACTCCTACACGATGCTGTCGGTGCTGACGGCGC
TTCAGAAACGGGCGCCGATCCAGTTCAAGCTGATCGCGATGAACCTGGACCAGAAGCAGCCCGGCTTTCCCGAGCATGTG
CTGCCGGCATACCTGAAGTCACTCGGCGTGGAGTATGTGATCGTCGAGGCGGACACCTACTCGATCGTCAAGGAGAAGGT
GCCCGAGGGCAAGACGACCTGCTCATTGTGCTCGCGCCTGCGCCGCGGCGTGATCTATCGCACGGCCAAGGAGCTGGGCG
CCAACAAGATCGCGCTGGGCCATCACCGCGACGACATCGTCAACACGTTCTTCCTGAACATGTTCTTCGGCGGCAAGATG
AAGGCGATGCCGCCGAAGCTGGCCACCGACGACGGCCAGCACATCGTGATCAGGCCGCTGGCGTACTGCGCGGAGAAGGA
CATCGCGTCGTATGCGCGTGCCATGGCGTTCCCGATTATCCCGTGCAACCTGTGCGGCTCGCAGGAAAACCTGCAGCGCA
AGAAGGTGAGCGAAATGCTGCAGGCGTGGGAGCGCGAGAACCCGGGCCGGATCGACAACATCTTCGCGGCACTACGCAAC
GTGGTGCCGTCGCACCTGGCCGATACGGACCTGTTCCCGTTCACGGGGCTGGCTACCGGGTTGGCCAAGATCGACGAGGC
ATCGCTGTTCGGCGAGACCACGTTCACGCAGCAGGCGCTGACCTTCACGGGCAATGTCGAGGCGAACCGGATGGAGTTCG
TGCGTTTCGACAAAATCCAGAAGGTCCAGGAGGCGCCGGAGCGAGCTTCGGAACCGTCGGCCTGA

Upstream 100 bases:

>100_bases
CGAGCGGGACCGCGCAGCGCCGGGCGGTGGGCTCCAGTAGAATGTTGGCCCGTTGAAACCGGGCCATTGTTCAGCGCCCG
TACTGATCCAGGTATGTCAT

Downstream 100 bases:

>100_bases
CGCCTCCCCCCGCCTCCCCCAAAGGTTGGCTACTGTGACGCCCGCGTCGAGCGCCCGGGCTCGCGCATCAACGCCTGCAC
ATCCTCGATCCATCCCTGGA

Product: tRNA 2-thiocytidine biosynthesis protein TtcA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 314; Mature: 313

Protein sequence:

>314_residues
MSHSANFYRLETRLQSLTGKAIGDFGMIEDGDTVLVCMSGGKDSYTMLSVLTALQKRAPIQFKLIAMNLDQKQPGFPEHV
LPAYLKSLGVEYVIVEADTYSIVKEKVPEGKTTCSLCSRLRRGVIYRTAKELGANKIALGHHRDDIVNTFFLNMFFGGKM
KAMPPKLATDDGQHIVIRPLAYCAEKDIASYARAMAFPIIPCNLCGSQENLQRKKVSEMLQAWERENPGRIDNIFAALRN
VVPSHLADTDLFPFTGLATGLAKIDEASLFGETTFTQQALTFTGNVEANRMEFVRFDKIQKVQEAPERASEPSA

Sequences:

>Translated_314_residues
MSHSANFYRLETRLQSLTGKAIGDFGMIEDGDTVLVCMSGGKDSYTMLSVLTALQKRAPIQFKLIAMNLDQKQPGFPEHV
LPAYLKSLGVEYVIVEADTYSIVKEKVPEGKTTCSLCSRLRRGVIYRTAKELGANKIALGHHRDDIVNTFFLNMFFGGKM
KAMPPKLATDDGQHIVIRPLAYCAEKDIASYARAMAFPIIPCNLCGSQENLQRKKVSEMLQAWERENPGRIDNIFAALRN
VVPSHLADTDLFPFTGLATGLAKIDEASLFGETTFTQQALTFTGNVEANRMEFVRFDKIQKVQEAPERASEPSA
>Mature_313_residues
SHSANFYRLETRLQSLTGKAIGDFGMIEDGDTVLVCMSGGKDSYTMLSVLTALQKRAPIQFKLIAMNLDQKQPGFPEHVL
PAYLKSLGVEYVIVEADTYSIVKEKVPEGKTTCSLCSRLRRGVIYRTAKELGANKIALGHHRDDIVNTFFLNMFFGGKMK
AMPPKLATDDGQHIVIRPLAYCAEKDIASYARAMAFPIIPCNLCGSQENLQRKKVSEMLQAWERENPGRIDNIFAALRNV
VPSHLADTDLFPFTGLATGLAKIDEASLFGETTFTQQALTFTGNVEANRMEFVRFDKIQKVQEAPERASEPSA

Specific function: Required for the thiolation of cytidine in position 32 of tRNA, to form 2-thiocytidine (s(2)C32)

COG id: COG0037

COG function: function code D; Predicted ATPase of the PP-loop superfamily implicated in cell cycle control

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TtcA family

Homologues:

Organism=Escherichia coli, GI1787606, Length=296, Percent_Identity=61.4864864864865, Blast_Score=399, Evalue=1e-112,
Organism=Caenorhabditis elegans, GI193211090, Length=224, Percent_Identity=32.5892857142857, Blast_Score=125, Evalue=3e-29,
Organism=Caenorhabditis elegans, GI17540090, Length=184, Percent_Identity=28.2608695652174, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24651972, Length=225, Percent_Identity=26.2222222222222, Blast_Score=71, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TTCA_RALME (Q1LS06)

Other databases:

- EMBL:   CP000352
- RefSeq:   YP_582339.1
- ProteinModelPortal:   Q1LS06
- STRING:   Q1LS06
- GeneID:   4036969
- GenomeReviews:   CP000352_GR
- KEGG:   rme:Rmet_0184
- eggNOG:   COG0037
- HOGENOM:   HBG347038
- OMA:   EHILPEY
- PhylomeDB:   Q1LS06
- ProtClustDB:   PRK10696
- BioCyc:   RMET266264:RMET_0184-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01850
- InterPro:   IPR012089
- InterPro:   IPR014729
- InterPro:   IPR011063
- Gene3D:   G3DSA:3.40.50.620
- PIRSF:   PIRSF004976

Pfam domain/function: PF01171 ATP_bind_3

EC number: NA

Molecular weight: Translated: 34866; Mature: 34734

Theoretical pI: Translated: 7.83; Mature: 7.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSHSANFYRLETRLQSLTGKAIGDFGMIEDGDTVLVCMSGGKDSYTMLSVLTALQKRAPI
CCCCCHHHHHHHHHHHHHCCHHCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCC
QFKLIAMNLDQKQPGFPEHVLPAYLKSLGVEYVIVEADTYSIVKEKVPEGKTTCSLCSRL
EEEEEEEECCCCCCCCCHHHHHHHHHHCCCCEEEEECCHHHHHHHHCCCCCHHHHHHHHH
RRGVIYRTAKELGANKIALGHHRDDIVNTFFLNMFFGGKMKAMPPKLATDDGQHIVIRPL
HHHHHHHHHHHHCCCCEEECCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEHHH
AYCAEKDIASYARAMAFPIIPCNLCGSQENLQRKKVSEMLQAWERENPGRIDNIFAALRN
HHHHHHHHHHHHHHHHCCCEECCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
VVPSHLADTDLFPFTGLATGLAKIDEASLFGETTFTQQALTFTGNVEANRMEFVRFDKIQ
HHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHEEEECCCCCCHHHHHHHHHHH
KVQEAPERASEPSA
HHHHHHHHCCCCCC
>Mature Secondary Structure 
SHSANFYRLETRLQSLTGKAIGDFGMIEDGDTVLVCMSGGKDSYTMLSVLTALQKRAPI
CCCCHHHHHHHHHHHHHCCHHCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCC
QFKLIAMNLDQKQPGFPEHVLPAYLKSLGVEYVIVEADTYSIVKEKVPEGKTTCSLCSRL
EEEEEEEECCCCCCCCCHHHHHHHHHHCCCCEEEEECCHHHHHHHHCCCCCHHHHHHHHH
RRGVIYRTAKELGANKIALGHHRDDIVNTFFLNMFFGGKMKAMPPKLATDDGQHIVIRPL
HHHHHHHHHHHHCCCCEEECCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEHHH
AYCAEKDIASYARAMAFPIIPCNLCGSQENLQRKKVSEMLQAWERENPGRIDNIFAALRN
HHHHHHHHHHHHHHHHCCCEECCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
VVPSHLADTDLFPFTGLATGLAKIDEASLFGETTFTQQALTFTGNVEANRMEFVRFDKIQ
HHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHEEEECCCCCCHHHHHHHHHHH
KVQEAPERASEPSA
HHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA