Click here to switch to the map view.

The map label for this gene is metF [H]

Identifier: 94309117

GI number: 94309117

Start: 179577

End: 180407

Strand: Direct

Name: metF [H]

Synonym: Rmet_0172

Alternate gene names: 94309117

Gene position: 179577-180407 (Clockwise)

Preceding gene: 94309116

Following gene: 94309118

Centisome position: 4.57

GC content: 64.98

Gene sequence:

>831_bases
ATGACTGATCGTTACTTCAGCTTCGAATTCTTCCCGCCCAAGACGGCGGAGGGCGCGGAGAAACTGCGCAATACGCGCGC
GCAGCTTGCGCCGCTGCAGCCCAAGTACATCTCGGTGACGTTCGGCGCCGGCGGTACCACGCAGCAGGGCACGCTCGATG
CCGTGCTGGAGATCCAGCGCGAAGGTATCGAGGCCGCGCCGCACTTGTCCTGCGTGGGCTCCACGCGCGACAGCATCCGG
CAGATTCTCCAGACCTATCGCGATGGCGGCATCCGCCACATCGTGGCCCTGCGCGGCGATATGCCGTCGGGCATGGGCGA
GATCGGCGAGTTCCGCTATGCCAACGAACTCGTCGAGTTCATCCGCGCGGAAACCGGTGACTGGTTCAACATCGAAGTGG
CCGCCTATCCCGAGTACCACCCGCAGGCCCGTTCGCCGCGTCACGACCTGGAAAACTTCGTGCGCAAGGTCAAGGCCGGC
GCGGACTCGGCGATCACGCAGTACTTCTTCAACGCCGATGCGTACTTCCGCTTCGTCGACGACGTGCGCGCGATGGGCGT
GGAAGTGCCGATCGTGCCCGGCATCATGCCGATCACGAACTACTCTCAGCTGATGCGCTTTTCCGAGATGTGCGGCGCCG
AAGTGCCGCGCTGGGTGGCCAAGCGGCTCGAGTCGTTCGGCGATGACAAGGAAGCAATCCGCGCCTTCGGCCTGGACGTG
GTGACCGACCTGTGCACGCGCCTGCTCGAAGCGGGCGTGCCGGGCCTGCACTTCTACACGCTCAACGCCGCCGGCGCGAC
CAAGGCGATCTGGCAGCGCCTGAAGCTGTAA

Upstream 100 bases:

>100_bases
ACAGCGTGATTGCGTGGATCCTGGCCAGCGTGCTGCTGGGCGAGCGCAACTGACGCGTTGAGACTCTTTTTCTGGGCGCC
GCCACGGCGGCGTGCCAACC

Downstream 100 bases:

>100_bases
TCCCCGATGCCCGCCGCGCGCCGCCCGGACCCATTCGTCGAGCACCTGCTCGAGCTGATGACGCCGCTGGCGGCCAACAT
CGGGGCCATCGCGGCCAAGC

Product: 5,10-methylenetetrahydrofolate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 276; Mature: 275

Protein sequence:

>276_residues
MTDRYFSFEFFPPKTAEGAEKLRNTRAQLAPLQPKYISVTFGAGGTTQQGTLDAVLEIQREGIEAAPHLSCVGSTRDSIR
QILQTYRDGGIRHIVALRGDMPSGMGEIGEFRYANELVEFIRAETGDWFNIEVAAYPEYHPQARSPRHDLENFVRKVKAG
ADSAITQYFFNADAYFRFVDDVRAMGVEVPIVPGIMPITNYSQLMRFSEMCGAEVPRWVAKRLESFGDDKEAIRAFGLDV
VTDLCTRLLEAGVPGLHFYTLNAAGATKAIWQRLKL

Sequences:

>Translated_276_residues
MTDRYFSFEFFPPKTAEGAEKLRNTRAQLAPLQPKYISVTFGAGGTTQQGTLDAVLEIQREGIEAAPHLSCVGSTRDSIR
QILQTYRDGGIRHIVALRGDMPSGMGEIGEFRYANELVEFIRAETGDWFNIEVAAYPEYHPQARSPRHDLENFVRKVKAG
ADSAITQYFFNADAYFRFVDDVRAMGVEVPIVPGIMPITNYSQLMRFSEMCGAEVPRWVAKRLESFGDDKEAIRAFGLDV
VTDLCTRLLEAGVPGLHFYTLNAAGATKAIWQRLKL
>Mature_275_residues
TDRYFSFEFFPPKTAEGAEKLRNTRAQLAPLQPKYISVTFGAGGTTQQGTLDAVLEIQREGIEAAPHLSCVGSTRDSIRQ
ILQTYRDGGIRHIVALRGDMPSGMGEIGEFRYANELVEFIRAETGDWFNIEVAAYPEYHPQARSPRHDLENFVRKVKAGA
DSAITQYFFNADAYFRFVDDVRAMGVEVPIVPGIMPITNYSQLMRFSEMCGAEVPRWVAKRLESFGDDKEAIRAFGLDVV
TDLCTRLLEAGVPGLHFYTLNAAGATKAIWQRLKL

Specific function: Methionine biosynthesis. [C]

COG id: COG0685

COG function: function code E; 5,10-methylenetetrahydrofolate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methylenetetrahydrofolate reductase family [H]

Homologues:

Organism=Homo sapiens, GI87240000, Length=283, Percent_Identity=42.0494699646643, Blast_Score=205, Evalue=4e-53,
Organism=Escherichia coli, GI1790377, Length=269, Percent_Identity=44.2379182156134, Blast_Score=238, Evalue=2e-64,
Organism=Caenorhabditis elegans, GI25147436, Length=283, Percent_Identity=32.8621908127208, Blast_Score=171, Evalue=4e-43,
Organism=Caenorhabditis elegans, GI25147441, Length=283, Percent_Identity=32.8621908127208, Blast_Score=171, Evalue=4e-43,
Organism=Saccharomyces cerevisiae, GI6321313, Length=282, Percent_Identity=36.5248226950355, Blast_Score=176, Evalue=4e-45,
Organism=Saccharomyces cerevisiae, GI6325234, Length=284, Percent_Identity=34.8591549295775, Blast_Score=161, Evalue=1e-40,
Organism=Drosophila melanogaster, GI24662658, Length=227, Percent_Identity=28.1938325991189, Blast_Score=85, Evalue=4e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003171
- InterPro:   IPR004620 [H]

Pfam domain/function: PF02219 MTHFR [H]

EC number: =1.5.1.20 [H]

Molecular weight: Translated: 30898; Mature: 30766

Theoretical pI: Translated: 6.05; Mature: 6.05

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDRYFSFEFFPPKTAEGAEKLRNTRAQLAPLQPKYISVTFGAGGTTQQGTLDAVLEIQR
CCCCEEEEEECCCCCCHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHH
EGIEAAPHLSCVGSTRDSIRQILQTYRDGGIRHIVALRGDMPSGMGEIGEFRYANELVEF
HCCCCCCCHHHHCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHH
IRAETGDWFNIEVAAYPEYHPQARSPRHDLENFVRKVKAGADSAITQYFFNADAYFRFVD
HHHCCCCEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHCCHHHHHHHH
DVRAMGVEVPIVPGIMPITNYSQLMRFSEMCGAEVPRWVAKRLESFGDDKEAIRAFGLDV
HHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHCCCHHHHHHHHHHHH
VTDLCTRLLEAGVPGLHFYTLNAAGATKAIWQRLKL
HHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHCC
>Mature Secondary Structure 
TDRYFSFEFFPPKTAEGAEKLRNTRAQLAPLQPKYISVTFGAGGTTQQGTLDAVLEIQR
CCCEEEEEECCCCCCHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHH
EGIEAAPHLSCVGSTRDSIRQILQTYRDGGIRHIVALRGDMPSGMGEIGEFRYANELVEF
HCCCCCCCHHHHCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHH
IRAETGDWFNIEVAAYPEYHPQARSPRHDLENFVRKVKAGADSAITQYFFNADAYFRFVD
HHHCCCCEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHCCHHHHHHHH
DVRAMGVEVPIVPGIMPITNYSQLMRFSEMCGAEVPRWVAKRLESFGDDKEAIRAFGLDV
HHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHCCCHHHHHHHHHHHH
VTDLCTRLLEAGVPGLHFYTLNAAGATKAIWQRLKL
HHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9537320 [H]