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The map label for this gene is thiG

Identifier: 94309110

GI number: 94309110

Start: 173495

End: 174343

Strand: Direct

Name: thiG

Synonym: Rmet_0165

Alternate gene names: 94309110

Gene position: 173495-174343 (Clockwise)

Preceding gene: 94309109

Following gene: 94309111

Centisome position: 4.42

GC content: 69.38

Gene sequence:

>849_bases
ATGACATTCGAACCAACTGAAACCCTGCGCGATCCGTTCGTGCTGTACGGGGAATCGTTCGCGTCGCGCCTGCTGCTCGG
CACGGCACGCTACCCGTCCCCCGCCACGCTGCAGGCGGCCGTGGAAGCCTCGCGTCCGGCCATGATCACGGTGGCGCTGC
GCCGTCAGACCGCCGTGGGCAGCGGTGATGGCCAGACGCTCGGCGGCGAGACCTTCTGGCAGATGCTGCGCACGCTGGGC
GTGCCGGTGCTGCCCAATACCGCCGGCTGCTTCACGGCGCAGGAAGTGATCACCACGGCGATGATGGCCCGCGAGGTATT
CGAGACCGACTGGATCAAGCTGGAACTGATCGGCGACGACTACACGCTGCAGCCCGACACGCTCAACATGCCTGCCGTGG
CCGAGACGCTGGTCAAGGAAGGCTTCAAGGTGCTGCCGTACTGCACCGAGGACCTCGTGCTGTGTCGCCGCCTGCTAGAC
GTCGGTTGCCAGGCGCTGATGCCGTGGGCTGCCCCGATCGGCACAGGCCGTGGCGCGGTGAACCCGCACGCGATGCGCAC
GCTGCGCGACCGTCTGCCCGACACCCCGCTGATCGTCGATGCCGGCCTCGGCCTGCCGTCGCATGCGGCGCAGGTGCTGG
AGTGGGGCTACGACGGTGTGCTGCTCAATACGGCCGTGGCCCAGTCCGCGTATCCGGTGAACATGGCGCGCGCGTTCGCG
CTGGCCGTGGAAGCCGGGCGCACCGCCTACCTGGCCGGCCCGATGCCCGAGCGCGAGGTGGCCCAGGCCAGCACGCCTGT
GGTGGGGATGCCGTTCTGGCACGCCGAAGGCGCGGAGGACCGCGCATGA

Upstream 100 bases:

>100_bases
AACGGCCAGTTCGTCCCGCGCACCCGCCATGCGGCCACGCTGTTGGCCGCGGGCGACCAGATCGATCTCGTGCAACCCGT
GACGGGAGGCTAAGTCCCTT

Downstream 100 bases:

>100_bases
CACGTCAGGGTGTTGCCGCGGCCATCGACGCCGCGCTGTTCGATGACTTGATGGCGCGTTACGCCGCGATGTTTGGCCGT
GACGAAACGCCATGGCGCAT

Product: thiazole synthase

Products: 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate; 4-hydroxy-benzyl-alcohol; C1 of tyrosine; ThiS protein [C]

Alternate protein names: NA

Number of amino acids: Translated: 282; Mature: 281

Protein sequence:

>282_residues
MTFEPTETLRDPFVLYGESFASRLLLGTARYPSPATLQAAVEASRPAMITVALRRQTAVGSGDGQTLGGETFWQMLRTLG
VPVLPNTAGCFTAQEVITTAMMAREVFETDWIKLELIGDDYTLQPDTLNMPAVAETLVKEGFKVLPYCTEDLVLCRRLLD
VGCQALMPWAAPIGTGRGAVNPHAMRTLRDRLPDTPLIVDAGLGLPSHAAQVLEWGYDGVLLNTAVAQSAYPVNMARAFA
LAVEAGRTAYLAGPMPEREVAQASTPVVGMPFWHAEGAEDRA

Sequences:

>Translated_282_residues
MTFEPTETLRDPFVLYGESFASRLLLGTARYPSPATLQAAVEASRPAMITVALRRQTAVGSGDGQTLGGETFWQMLRTLG
VPVLPNTAGCFTAQEVITTAMMAREVFETDWIKLELIGDDYTLQPDTLNMPAVAETLVKEGFKVLPYCTEDLVLCRRLLD
VGCQALMPWAAPIGTGRGAVNPHAMRTLRDRLPDTPLIVDAGLGLPSHAAQVLEWGYDGVLLNTAVAQSAYPVNMARAFA
LAVEAGRTAYLAGPMPEREVAQASTPVVGMPFWHAEGAEDRA
>Mature_281_residues
TFEPTETLRDPFVLYGESFASRLLLGTARYPSPATLQAAVEASRPAMITVALRRQTAVGSGDGQTLGGETFWQMLRTLGV
PVLPNTAGCFTAQEVITTAMMAREVFETDWIKLELIGDDYTLQPDTLNMPAVAETLVKEGFKVLPYCTEDLVLCRRLLDV
GCQALMPWAAPIGTGRGAVNPHAMRTLRDRLPDTPLIVDAGLGLPSHAAQVLEWGYDGVLLNTAVAQSAYPVNMARAFAL
AVEAGRTAYLAGPMPEREVAQASTPVVGMPFWHAEGAEDRA

Specific function: Catalyzes the rearrangement of 1-deoxy-D-xylulose 5- phosphate (DXP) to produce the thiazole phosphate moiety of thiamine. Sulfur is provided by the thiocarboxylate moiety of the carrier protein ThiS. In vitro, sulfur can be provided by H(2)S

COG id: COG2022

COG function: function code H; Uncharacterized enzyme of thiazole biosynthesis

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiG family

Homologues:

Organism=Escherichia coli, GI48994993, Length=253, Percent_Identity=43.8735177865613, Blast_Score=197, Evalue=8e-52,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THIG_RALME (Q1LS25)

Other databases:

- EMBL:   CP000352
- RefSeq:   YP_582320.1
- ProteinModelPortal:   Q1LS25
- SMR:   Q1LS25
- STRING:   Q1LS25
- GeneID:   4036950
- GenomeReviews:   CP000352_GR
- KEGG:   rme:Rmet_0165
- eggNOG:   COG2022
- HOGENOM:   HBG296821
- OMA:   VAIRRTN
- PhylomeDB:   Q1LS25
- ProtClustDB:   PRK00208
- BioCyc:   RMET266264:RMET_0165-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00443
- InterPro:   IPR013785
- InterPro:   IPR008867
- Gene3D:   G3DSA:3.20.20.70

Pfam domain/function: PF05690 ThiG; SSF110399 ThiG

EC number: NA

Molecular weight: Translated: 30408; Mature: 30277

Theoretical pI: Translated: 4.56; Mature: 4.56

Prosite motif: NA

Important sites: ACT_SITE 113-113 BINDING 174-174

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTFEPTETLRDPFVLYGESFASRLLLGTARYPSPATLQAAVEASRPAMITVALRRQTAVG
CCCCCCHHHCCCEEEECHHHHHHHHHHCCCCCCCHHHHHHHHCCCCEEEEEEEEHHHCCC
SGDGQTLGGETFWQMLRTLGVPVLPNTAGCFTAQEVITTAMMAREVFETDWIKLELIGDD
CCCCCCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEECCC
YTLQPDTLNMPAVAETLVKEGFKVLPYCTEDLVLCRRLLDVGCQALMPWAAPIGTGRGAV
EEECCCCCCCCHHHHHHHHCCCEECCCCHHHHHHHHHHHHHHHHHHHCHHCCCCCCCCCC
NPHAMRTLRDRLPDTPLIVDAGLGLPSHAAQVLEWGYDGVLLNTAVAQSAYPVNMARAFA
CHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHCCCCCEEEHHHHHHCCCCHHHHHHHH
LAVEAGRTAYLAGPMPEREVAQASTPVVGMPFWHAEGAEDRA
HHHHCCCEEEEECCCCHHHHHHCCCCEEECCCCCCCCCCCCC
>Mature Secondary Structure 
TFEPTETLRDPFVLYGESFASRLLLGTARYPSPATLQAAVEASRPAMITVALRRQTAVG
CCCCCHHHCCCEEEECHHHHHHHHHHCCCCCCCHHHHHHHHCCCCEEEEEEEEHHHCCC
SGDGQTLGGETFWQMLRTLGVPVLPNTAGCFTAQEVITTAMMAREVFETDWIKLELIGDD
CCCCCCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEECCC
YTLQPDTLNMPAVAETLVKEGFKVLPYCTEDLVLCRRLLDVGCQALMPWAAPIGTGRGAV
EEECCCCCCCCHHHHHHHHCCCEECCCCHHHHHHHHHHHHHHHHHHHCHHCCCCCCCCCC
NPHAMRTLRDRLPDTPLIVDAGLGLPSHAAQVLEWGYDGVLLNTAVAQSAYPVNMARAFA
CHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHCCCCCEEEHHHHHHCCCCHHHHHHHH
LAVEAGRTAYLAGPMPEREVAQASTPVVGMPFWHAEGAEDRA
HHHHCCCEEEEECCCCHHHHHHCCCCEEECCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: deoxyxylulose-5-phosphate; ThiS-COSH; L-tyrosine [C]

Specific reaction: deoxyxylulose-5-phosphate + ThiS-COSH + L-tyrosine = 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate + 4-hydroxy-benzyl-alcohol + C1 of tyrosine + ThiS protein [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA