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The map label for this gene is thiE [H]

Identifier: 94309111

GI number: 94309111

Start: 174340

End: 175470

Strand: Direct

Name: thiE [H]

Synonym: Rmet_0166

Alternate gene names: 94309111

Gene position: 174340-175470 (Clockwise)

Preceding gene: 94309110

Following gene: 94309112

Centisome position: 4.44

GC content: 68.61

Gene sequence:

>1131_bases
ATGACACGTCAGGGTGTTGCCGCGGCCATCGACGCCGCGCTGTTCGATGACTTGATGGCGCGTTACGCCGCGATGTTTGG
CCGTGACGAAACGCCATGGCGCATCTGGTCGGCGGCGAATGCTCCCGCCGCGCTCGATCGCCATGACGTGGTGCTGTCCG
ATGGCGAGGCCACGGGGGATCTGATCGATCGCGTGGTGGCCGCCGATGCTGTGCTGATCGAATCGGAGCGCGAAGGCGGC
CGCTGGATCGACACCGTGCGCTCGCCGATGGGTACCTGGGTGCTCGACGTGCGGGCCGACGACGACACGCCGCATTCGGC
CGCGTTCGTGGCCGTGCTGCGCGCCGCGTTGTCGCTGCACTTTCCGGCGCACGACGCCCTGTGCATTGCCCGCGCCTGGC
TGCCGGGCGCCACGGCCTGGCCGAACGATTTCGCGCGCTTTCCGCAGGTGCGACATGCGGCGCTGGTGCCGCCGGGCCAG
ACCGCGCAGCCCTTCGCGCCTTGTCCGCCGGATCTCGGTCTCTATGCCGTGATGCCGAGCGCGGCGTGGATCGAGACGCT
CGTGCCGCTGGCCGTGCCGACCGTGCAGCTGCGCTTCAAGTCTGGCGATGCCGACGCGGTGAAGCATGAAGTGGCACGCG
CCGCGAAGGCCGCCAAGGGTTCGCAGTCGCGCCTGTTCATCAACGATCACTGGCGTGTGGCGATCGATCACCACGCAGCT
TGCGGTGGCGACAGCGGCATCTACGGGATTCATCTCGGGCAGGAGGATCTGGACGACGCCGACCTCGACGCGATCCGCGC
GTCGGGCCTGCGCCTCGGGGTGTCCACGCACGGCTATGCGGAAATGCTGCGCGTGGCCGCGATCTCGCCGAGCTACCTCG
CGCTTGGCGCGATCTTCCCGACCACCACCAAGGTCATGCCAACCCAGCCGCAGGGCATGGGCCGCTTCCAGTCCTACGTG
GCGTTAATGCAGCCTGTGATCCCGTCGCTGGTCGGCATCGGCGGGGTGAACGCAGGCAACATGGGGGAAGTGCTTGCGGT
GGGCGTCGGCAGCGCTGCCGTGGTGCGTGCGATCACGGAAGCCAGCGATGTGCCGGCGGCGGTGGCGGGGCTCAACGCCT
TGTTTGCCTGA

Upstream 100 bases:

>100_bases
CCTACCTGGCCGGCCCGATGCCCGAGCGCGAGGTGGCCCAGGCCAGCACGCCTGTGGTGGGGATGCCGTTCTGGCACGCC
GAAGGCGCGGAGGACCGCGC

Downstream 100 bases:

>100_bases
TCCGGACGTAGACTTTGGGCGCTGACCGGCCAGCCCGGCTGGTGGCGCCCGGCAATTGCGCATATCCTCTTTCGCTCACC
ATTAAAGGACTGAGGTTCCG

Product: thiamine monophosphate synthase

Products: NA

Alternate protein names: TMP pyrophosphorylase; TMP-PPase; Thiamine-phosphate synthase [H]

Number of amino acids: Translated: 376; Mature: 375

Protein sequence:

>376_residues
MTRQGVAAAIDAALFDDLMARYAAMFGRDETPWRIWSAANAPAALDRHDVVLSDGEATGDLIDRVVAADAVLIESEREGG
RWIDTVRSPMGTWVLDVRADDDTPHSAAFVAVLRAALSLHFPAHDALCIARAWLPGATAWPNDFARFPQVRHAALVPPGQ
TAQPFAPCPPDLGLYAVMPSAAWIETLVPLAVPTVQLRFKSGDADAVKHEVARAAKAAKGSQSRLFINDHWRVAIDHHAA
CGGDSGIYGIHLGQEDLDDADLDAIRASGLRLGVSTHGYAEMLRVAAISPSYLALGAIFPTTTKVMPTQPQGMGRFQSYV
ALMQPVIPSLVGIGGVNAGNMGEVLAVGVGSAAVVRAITEASDVPAAVAGLNALFA

Sequences:

>Translated_376_residues
MTRQGVAAAIDAALFDDLMARYAAMFGRDETPWRIWSAANAPAALDRHDVVLSDGEATGDLIDRVVAADAVLIESEREGG
RWIDTVRSPMGTWVLDVRADDDTPHSAAFVAVLRAALSLHFPAHDALCIARAWLPGATAWPNDFARFPQVRHAALVPPGQ
TAQPFAPCPPDLGLYAVMPSAAWIETLVPLAVPTVQLRFKSGDADAVKHEVARAAKAAKGSQSRLFINDHWRVAIDHHAA
CGGDSGIYGIHLGQEDLDDADLDAIRASGLRLGVSTHGYAEMLRVAAISPSYLALGAIFPTTTKVMPTQPQGMGRFQSYV
ALMQPVIPSLVGIGGVNAGNMGEVLAVGVGSAAVVRAITEASDVPAAVAGLNALFA
>Mature_375_residues
TRQGVAAAIDAALFDDLMARYAAMFGRDETPWRIWSAANAPAALDRHDVVLSDGEATGDLIDRVVAADAVLIESEREGGR
WIDTVRSPMGTWVLDVRADDDTPHSAAFVAVLRAALSLHFPAHDALCIARAWLPGATAWPNDFARFPQVRHAALVPPGQT
AQPFAPCPPDLGLYAVMPSAAWIETLVPLAVPTVQLRFKSGDADAVKHEVARAAKAAKGSQSRLFINDHWRVAIDHHAAC
GGDSGIYGIHLGQEDLDDADLDAIRASGLRLGVSTHGYAEMLRVAAISPSYLALGAIFPTTTKVMPTQPQGMGRFQSYVA
LMQPVIPSLVGIGGVNAGNMGEVLAVGVGSAAVVRAITEASDVPAAVAGLNALFA

Specific function: Condenses 4-methyl-5-(beta-hydroxyethyl)thiazole monophosphate (THZ-P) and 2-methyl-4-amino-5-hydroxymethyl pyrimidine pyrophosphate (HMP-PP) to form thiamine monophosphate (TMP) [H]

COG id: COG0352

COG function: function code H; Thiamine monophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TMP-PPase family [H]

Homologues:

Organism=Escherichia coli, GI1790426, Length=210, Percent_Identity=47.6190476190476, Blast_Score=165, Evalue=4e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR022998
- InterPro:   IPR003733 [H]

Pfam domain/function: PF02581 TMP-TENI [H]

EC number: =2.5.1.3 [H]

Molecular weight: Translated: 39588; Mature: 39457

Theoretical pI: Translated: 5.32; Mature: 5.32

Prosite motif: PS00579 RIBOSOMAL_L29

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRQGVAAAIDAALFDDLMARYAAMFGRDETPWRIWSAANAPAALDRHDVVLSDGEATGD
CCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCEEEECCCCHHH
LIDRVVAADAVLIESEREGGRWIDTVRSPMGTWVLDVRADDDTPHSAAFVAVLRAALSLH
HHHHHHHHHHEEEECCCCCCCHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHC
FPAHDALCIARAWLPGATAWPNDFARFPQVRHAALVPPGQTAQPFAPCPPDLGLYAVMPS
CCCCHHHHHHHHHCCCCCCCCHHHHHCCCHHHEEECCCCCCCCCCCCCCCCCCEEEECCC
AAWIETLVPLAVPTVQLRFKSGDADAVKHEVARAAKAAKGSQSRLFINDHWRVAIDHHAA
HHHHHHHHHHHCCEEEEEEECCCHHHHHHHHHHHHHHCCCCCCEEEEECCEEEEEECCCC
CGGDSGIYGIHLGQEDLDDADLDAIRASGLRLGVSTHGYAEMLRVAAISPSYLALGAIFP
CCCCCCEEEEECCCCCCCCCHHHHHHHCCEEEECCCCCHHHHHHHHHCCCCHHHHHHHCC
TTTKVMPTQPQGMGRFQSYVALMQPVIPSLVGIGGVNAGNMGEVLAVGVGSAAVVRAITE
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCHHHHHHHHHH
ASDVPAAVAGLNALFA
HCCCCHHHHHHHHHCC
>Mature Secondary Structure 
TRQGVAAAIDAALFDDLMARYAAMFGRDETPWRIWSAANAPAALDRHDVVLSDGEATGD
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCEEEECCCCHHH
LIDRVVAADAVLIESEREGGRWIDTVRSPMGTWVLDVRADDDTPHSAAFVAVLRAALSLH
HHHHHHHHHHEEEECCCCCCCHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHC
FPAHDALCIARAWLPGATAWPNDFARFPQVRHAALVPPGQTAQPFAPCPPDLGLYAVMPS
CCCCHHHHHHHHHCCCCCCCCHHHHHCCCHHHEEECCCCCCCCCCCCCCCCCCEEEECCC
AAWIETLVPLAVPTVQLRFKSGDADAVKHEVARAAKAAKGSQSRLFINDHWRVAIDHHAA
HHHHHHHHHHHCCEEEEEEECCCHHHHHHHHHHHHHHCCCCCCEEEEECCEEEEEECCCC
CGGDSGIYGIHLGQEDLDDADLDAIRASGLRLGVSTHGYAEMLRVAAISPSYLALGAIFP
CCCCCCEEEEECCCCCCCCCHHHHHHHCCEEEECCCCCHHHHHHHHHCCCCHHHHHHHCC
TTTKVMPTQPQGMGRFQSYVALMQPVIPSLVGIGGVNAGNMGEVLAVGVGSAAVVRAITE
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCHHHHHHHHHH
ASDVPAAVAGLNALFA
HCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA