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The map label for this gene is thiC [H]

Identifier: 94309107

GI number: 94309107

Start: 170011

End: 171888

Strand: Direct

Name: thiC [H]

Synonym: Rmet_0162

Alternate gene names: 94309107

Gene position: 170011-171888 (Clockwise)

Preceding gene: 94309104

Following gene: 94309108

Centisome position: 4.33

GC content: 64.8

Gene sequence:

>1878_bases
ATGGCCCGTACTGCCCCCGCTGCTTCGTTTGAATCGCTCGAAAGCGATCTCGACCAGAAATTCGCCTACCCGGCTTCCAG
CAAGACCTATATCACCGGCAGCCGGCCGGACATCCGCGTGCCGCTGCGCACGATCCTGCAGACCGCCACGCGCACCGAGA
AGGGCGAGATGGCGAACCCGCCGATTCCCGTCTATGACACCTCGGGACCGTACAGCGATCCGGACGTGCATATCGACCTG
AAGGCCGGCCTGCCGCCCGTCCGCGCCAAGTGGATCGAAGAGCGCAACGACACCGAGGTGCTGACTGGCCTGTCGTCGGA
GTACGGCCTGGCACGCGCCAATGACCCCGCCACGGCACACCTGCGCTTCGCGCAACTGACGAACCCGCGCCGCGCCAAGG
CCGGCGCGAACGTGTCGCAGATGCACTACGCACGCAAGGGCATCATCACGCCCGAAATGGAATACGTGGCGCTGCGCGAA
TCGCTGAACCTGCAGGCGCTCTACGACAAGCCCGAGTACAAGGCGCTGCTGCGACAGCATCCGGGCAACGCCCTGGGCGC
CGCTCTGCCGCTGCGTCCGGAAGACATGACGCCGGAATTCGTGCGCCGCGAAGTGGCCGCCGGCCGCGCCATCATTCCGG
CCAACATCAACCACACGGAACTGGAGCCGATGGCGATCGGCCGCAACTTCCGCGTCAAGATCAACGGCAACCTGGGCAAT
TCGGCGGTGACGTCGTCGCTGGCCGAGGAAGTGGAAAAGATGGTGTGGTCGATCCGCTGGGGCGCCGACACCATCATGGA
TCTGTCCACCGGCAAGCACATCCACGAAACGCGCGAGTGGATCCTGCGCAACTCGCCGGTGCCCATCGGCACGGTGCCCA
TCTACCAGGCGCTGGACAAGACCGGCGGTATCGCCGAGGACCTGACCTGGGAGATGTTCCGCGACACGCTGATCGAGCAG
GCAGAGCAGGGCGTGGACTACTTCACGATCCACGCCGGCGTGCTGCTGCGCTACGTGCCGATGACGGCCGACCGCGTGAC
CGGCATCGTCTCGCGCGGTGGTTCGATCATGGCCAAGTGGTGCCTGGCGCACCACAAGGAAAATTTCCTGTACACGCACT
TCGACGAGATCTGCGAAATCATGAAGGCGTACGACGTGTCGTTCAGCCTGGGTGATGGTCTGCGTCCGGGCTGTATCGCC
GATTCGAACGATGACGCCCAGTTCGGCGAACTGCGCACGCTTGGCGAACTGACCGCCAAGGCGTGGGAGCACGACGTGCA
GGTGATGATCGAAGGCCCGGGCCACGTGCCGCTGCAGCGCATCCAGGCCAACATGGACGAAGAGCTCAAGCACTGCTACG
AAGCGCCGTTCTACACGCTGGGACCGCTGGTCACCGACATCGCGCCGGGCTACGACCACATCACCAGCGGCATTGGCGCC
GCCAACATCGGCTGGATGGGCACCGCGATGCTGTGCTACGTCACGCCGAAGGAGCACCTGGGCCTGCCGGACAAGGAAGA
CGTGCGCGAGGGCATCATCACGTACAAGATCGCCGCGCACGCCGCCGACCTGGCCAAGGGCTGGCCCGGCGCGCAGCTCC
GCGACAACGCGCTGTCCAAGGCGCGCTTCGAGTTCCGCTGGGAAGACCAGTTCAACCTCGGTCTCGATCCCGAGCGCGCG
CGTTCGTTCCACGACGCCACGCTGCCGGCCGAAGGCGCCAAGATCGCCCACTTCTGCTCGATGTGCGGGCCGAAGTTCTG
CTCGATGAAGATCACGCAGGAAGTGCGCGACTACGCGGCATCGCTGCCGGAAGCCGAACGCGGAATGCAGGAGAAGTCGA
TTGAGTTCGTGAAGACGGGCTCGAAGATCTACTCGTAA

Upstream 100 bases:

>100_bases
TTGATCCGCACAAACGGGACACCGCCGCCGGCCATGCCTCGCGACGGACCTCACAGGGCTCCTGGTTTCGTCCATCCCCG
CATGAGAGAGGACGATACCC

Downstream 100 bases:

>100_bases
GCCGCTATCTGCGCCGACGTGCAGACGGGTGCGGCCCTTGTGGCCGCGCCCGGCAGTATCCGCAGTACCCGTAGCACCTG
AGTCCCGCGCGCGGCGCCCC

Product: thiamine biosynthesis protein ThiC

Products: @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine!; 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

Alternate protein names: Hydroxymethylpyrimidine phosphate synthase; HMP-P synthase; HMP-phosphate synthase; HMPP synthase; Thiamine biosynthesis protein thiC [H]

Number of amino acids: Translated: 625; Mature: 624

Protein sequence:

>625_residues
MARTAPAASFESLESDLDQKFAYPASSKTYITGSRPDIRVPLRTILQTATRTEKGEMANPPIPVYDTSGPYSDPDVHIDL
KAGLPPVRAKWIEERNDTEVLTGLSSEYGLARANDPATAHLRFAQLTNPRRAKAGANVSQMHYARKGIITPEMEYVALRE
SLNLQALYDKPEYKALLRQHPGNALGAALPLRPEDMTPEFVRREVAAGRAIIPANINHTELEPMAIGRNFRVKINGNLGN
SAVTSSLAEEVEKMVWSIRWGADTIMDLSTGKHIHETREWILRNSPVPIGTVPIYQALDKTGGIAEDLTWEMFRDTLIEQ
AEQGVDYFTIHAGVLLRYVPMTADRVTGIVSRGGSIMAKWCLAHHKENFLYTHFDEICEIMKAYDVSFSLGDGLRPGCIA
DSNDDAQFGELRTLGELTAKAWEHDVQVMIEGPGHVPLQRIQANMDEELKHCYEAPFYTLGPLVTDIAPGYDHITSGIGA
ANIGWMGTAMLCYVTPKEHLGLPDKEDVREGIITYKIAAHAADLAKGWPGAQLRDNALSKARFEFRWEDQFNLGLDPERA
RSFHDATLPAEGAKIAHFCSMCGPKFCSMKITQEVRDYAASLPEAERGMQEKSIEFVKTGSKIYS

Sequences:

>Translated_625_residues
MARTAPAASFESLESDLDQKFAYPASSKTYITGSRPDIRVPLRTILQTATRTEKGEMANPPIPVYDTSGPYSDPDVHIDL
KAGLPPVRAKWIEERNDTEVLTGLSSEYGLARANDPATAHLRFAQLTNPRRAKAGANVSQMHYARKGIITPEMEYVALRE
SLNLQALYDKPEYKALLRQHPGNALGAALPLRPEDMTPEFVRREVAAGRAIIPANINHTELEPMAIGRNFRVKINGNLGN
SAVTSSLAEEVEKMVWSIRWGADTIMDLSTGKHIHETREWILRNSPVPIGTVPIYQALDKTGGIAEDLTWEMFRDTLIEQ
AEQGVDYFTIHAGVLLRYVPMTADRVTGIVSRGGSIMAKWCLAHHKENFLYTHFDEICEIMKAYDVSFSLGDGLRPGCIA
DSNDDAQFGELRTLGELTAKAWEHDVQVMIEGPGHVPLQRIQANMDEELKHCYEAPFYTLGPLVTDIAPGYDHITSGIGA
ANIGWMGTAMLCYVTPKEHLGLPDKEDVREGIITYKIAAHAADLAKGWPGAQLRDNALSKARFEFRWEDQFNLGLDPERA
RSFHDATLPAEGAKIAHFCSMCGPKFCSMKITQEVRDYAASLPEAERGMQEKSIEFVKTGSKIYS
>Mature_624_residues
ARTAPAASFESLESDLDQKFAYPASSKTYITGSRPDIRVPLRTILQTATRTEKGEMANPPIPVYDTSGPYSDPDVHIDLK
AGLPPVRAKWIEERNDTEVLTGLSSEYGLARANDPATAHLRFAQLTNPRRAKAGANVSQMHYARKGIITPEMEYVALRES
LNLQALYDKPEYKALLRQHPGNALGAALPLRPEDMTPEFVRREVAAGRAIIPANINHTELEPMAIGRNFRVKINGNLGNS
AVTSSLAEEVEKMVWSIRWGADTIMDLSTGKHIHETREWILRNSPVPIGTVPIYQALDKTGGIAEDLTWEMFRDTLIEQA
EQGVDYFTIHAGVLLRYVPMTADRVTGIVSRGGSIMAKWCLAHHKENFLYTHFDEICEIMKAYDVSFSLGDGLRPGCIAD
SNDDAQFGELRTLGELTAKAWEHDVQVMIEGPGHVPLQRIQANMDEELKHCYEAPFYTLGPLVTDIAPGYDHITSGIGAA
NIGWMGTAMLCYVTPKEHLGLPDKEDVREGIITYKIAAHAADLAKGWPGAQLRDNALSKARFEFRWEDQFNLGLDPERAR
SFHDATLPAEGAKIAHFCSMCGPKFCSMKITQEVRDYAASLPEAERGMQEKSIEFVKTGSKIYS

Specific function: Catalyzes the synthesis of the hydroxymethylpyrimidine phosphate (HMP-P) moiety of thiamine from aminoimidazole ribotide (AIR) in a radical S-adenosyl-L-methionine (SAM)-dependent reaction [H]

COG id: COG0422

COG function: function code H; Thiamine biosynthesis protein ThiC

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiC family [H]

Homologues:

Organism=Escherichia coli, GI1790427, Length=608, Percent_Identity=69.7368421052632, Blast_Score=882, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002817 [H]

Pfam domain/function: PF01964 ThiC [H]

EC number: NA

Molecular weight: Translated: 69358; Mature: 69227

Theoretical pI: Translated: 5.82; Mature: 5.82

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARTAPAASFESLESDLDQKFAYPASSKTYITGSRPDIRVPLRTILQTATRTEKGEMANP
CCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCHHHHHHHHHCCCCCCCCCC
PIPVYDTSGPYSDPDVHIDLKAGLPPVRAKWIEERNDTEVLTGLSSEYGLARANDPATAH
CCCEEECCCCCCCCCEEEEECCCCCHHHHHHHHCCCCHHHHHCCCCCCCCCCCCCCCHHE
LRFAQLTNPRRAKAGANVSQMHYARKGIITPEMEYVALRESLNLQALYDKPEYKALLRQH
EEEHHHCCCHHHHCCCCHHHHHHHHCCCCCCCHHHHHHHHCCCEEEEECCHHHHHHHHHC
PGNALGAALPLRPEDMTPEFVRREVAAGRAIIPANINHTELEPMAIGRNFRVKINGNLGN
CCCHHCCCCCCCCCCCCHHHHHHHHHCCCEEEECCCCCCCCCCEEECCEEEEEECCCCCC
SAVTSSLAEEVEKMVWSIRWGADTIMDLSTGKHIHETREWILRNSPVPIGTVPIYQALDK
HHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHH
TGGIAEDLTWEMFRDTLIEQAEQGVDYFTIHAGVLLRYVPMTADRVTGIVSRGGSIMAKW
CCCCHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHCCCHHHHHHHHHCCCHHHHHH
CLAHHKENFLYTHFDEICEIMKAYDVSFSLGDGLRPGCIADSNDDAQFGELRTLGELTAK
HHHHHCCCCEEECHHHHHHHHHHHCCCEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
AWEHDVQVMIEGPGHVPLQRIQANMDEELKHCYEAPFYTLGPLVTDIAPGYDHITSGIGA
HHCCCEEEEEECCCCCCHHHHHCCHHHHHHHHHHCCCHHHHHHHHHCCCCHHHHHCCCCC
ANIGWMGTAMLCYVTPKEHLGLPDKEDVREGIITYKIAAHAADLAKGWPGAQLRDNALSK
CCCCHHCEEEEEEECCHHHCCCCCHHHHHCCCEEEEHHHHHHHHHCCCCCCCHHHHHHHH
ARFEFRWEDQFNLGLDPERARSFHDATLPAEGAKIAHFCSMCGPKFCSMKITQEVRDYAA
CEEEEEECCCCCCCCCHHHHHHHHCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHH
SLPEAERGMQEKSIEFVKTGSKIYS
CCCHHHHCCHHHHHHHHHCCHHHCC
>Mature Secondary Structure 
ARTAPAASFESLESDLDQKFAYPASSKTYITGSRPDIRVPLRTILQTATRTEKGEMANP
CCCCCCHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCHHHHHHHHHCCCCCCCCCC
PIPVYDTSGPYSDPDVHIDLKAGLPPVRAKWIEERNDTEVLTGLSSEYGLARANDPATAH
CCCEEECCCCCCCCCEEEEECCCCCHHHHHHHHCCCCHHHHHCCCCCCCCCCCCCCCHHE
LRFAQLTNPRRAKAGANVSQMHYARKGIITPEMEYVALRESLNLQALYDKPEYKALLRQH
EEEHHHCCCHHHHCCCCHHHHHHHHCCCCCCCHHHHHHHHCCCEEEEECCHHHHHHHHHC
PGNALGAALPLRPEDMTPEFVRREVAAGRAIIPANINHTELEPMAIGRNFRVKINGNLGN
CCCHHCCCCCCCCCCCCHHHHHHHHHCCCEEEECCCCCCCCCCEEECCEEEEEECCCCCC
SAVTSSLAEEVEKMVWSIRWGADTIMDLSTGKHIHETREWILRNSPVPIGTVPIYQALDK
HHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHH
TGGIAEDLTWEMFRDTLIEQAEQGVDYFTIHAGVLLRYVPMTADRVTGIVSRGGSIMAKW
CCCCHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHCCCHHHHHHHHHCCCHHHHHH
CLAHHKENFLYTHFDEICEIMKAYDVSFSLGDGLRPGCIADSNDDAQFGELRTLGELTAK
HHHHHCCCCEEECHHHHHHHHHHHCCCEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
AWEHDVQVMIEGPGHVPLQRIQANMDEELKHCYEAPFYTLGPLVTDIAPGYDHITSGIGA
HHCCCEEEEEECCCCCCHHHHHCCHHHHHHHHHHCCCHHHHHHHHHCCCCHHHHHCCCCC
ANIGWMGTAMLCYVTPKEHLGLPDKEDVREGIITYKIAAHAADLAKGWPGAQLRDNALSK
CCCCHHCEEEEEEECCHHHCCCCCHHHHHCCCEEEEHHHHHHHHHCCCCCCCHHHHHHHH
ARFEFRWEDQFNLGLDPERARSFHDATLPAEGAKIAHFCSMCGPKFCSMKITQEVRDYAA
CEEEEEECCCCCCCCCHHHHHHHHCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHH
SLPEAERGMQEKSIEFVKTGSKIYS
CCCHHHHCCHHHHHHHHHCCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ?; ?; 5'-phosphoribosyl-5-aminoimidazole [C]

Specific reaction: Synthesis of @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine! 5'-phosphoribosyl-5-aminoimidazole = 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11823852 [H]