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The map label for this gene is dapF

Identifier: 94309098

GI number: 94309098

Start: 157559

End: 158425

Strand: Reverse

Name: dapF

Synonym: Rmet_0153

Alternate gene names: 94309098

Gene position: 158425-157559 (Counterclockwise)

Preceding gene: 94309099

Following gene: 94309097

Centisome position: 4.03

GC content: 65.17

Gene sequence:

>867_bases
ATGAAACTCCAGTTCACCAAGATGCACGGCGCCGGCAACGATTTCATCGTGCTGGACGGCATTCACCAGAAGCTCGACCT
GACCGACGCGCAATGGCGCGCGCTGGCGAATCGCCATTTCGGCATCGGCGCCGACCAGATCCTGATCGTTGAAAAATCCT
CGCGCGACGATGTGGATTTCCGCTACCGGATCGTCAACGCGGACGGCGGCGAAGTCGAACACTGCGGCAACGGCGCCCGT
TGCTTCGTCCGCTTCGTCACGGATCGTGGCATGACCGACAAGCAGTCGGTCCGCGTGGAAGTCATGAACGGCGTCATCAC
GCTGAAGCTGCAGGACGACGGTCAGGTGACCGTGGACATGGGTGAGCCCGAACTCACGCCCGCCCGCGTGCCATTCATCG
CCGACGGCCTGCCGACGCGCGCCGAAGCCCAGGACACGCTCTATGGTCTCGAAGTCAACGGCCGCACCGAGTGGATCTCG
CCCGTGTCGATGGGCAATCCGCACGCGGTGCAGATCGTGGACGACGTAGAGCAATTCCCGGTGCTTCAGGACGGCCCGGT
CATCGAGCACCACAAGTCGTTCCCGAACCGGGTCAATGCCGGATTCATGCAGATCGTCGACCGCAATACGGTGCGGCTGC
GCGTGTTCGAACGCGGCGCGGGCGAAACGCTGGCGTGCGGCACGGGCGCCTGCGCGGCCGTGGTGGCTGGCATCCGCCGC
GGCTTGCTGGACTCTCCGGTCAAGGTGCACACCCACGGCGGCGACCTGACCATCGCGTGGCAAGGCGCTGGCCAGCCCGT
GCAGATGACGGGCCCCGCGACGACCGTGTTCGAAGGCACTATTGACCTCTCCACGCTGCCTGCCTGA

Upstream 100 bases:

>100_bases
ATGCCATCCGTCCGCGCGTGCCCGACTACTACTGGGTGCACAAACGCTTCAAGCACCGCCCGCCCGGCGAACCTGACATC
TACTGATTACAATTCCGGAC

Downstream 100 bases:

>100_bases
CCATTGAGGCTCGCAGACACACGGGGCCGGCCCGGCCCCGTCTTCGGGAGCACCAATGAAGCTGGACGATTTCTGCATCA
CCTCGGGCAAGGGGTTCAAG

Product: diaminopimelate epimerase

Products: NA

Alternate protein names: DAP epimerase

Number of amino acids: Translated: 288; Mature: 288

Protein sequence:

>288_residues
MKLQFTKMHGAGNDFIVLDGIHQKLDLTDAQWRALANRHFGIGADQILIVEKSSRDDVDFRYRIVNADGGEVEHCGNGAR
CFVRFVTDRGMTDKQSVRVEVMNGVITLKLQDDGQVTVDMGEPELTPARVPFIADGLPTRAEAQDTLYGLEVNGRTEWIS
PVSMGNPHAVQIVDDVEQFPVLQDGPVIEHHKSFPNRVNAGFMQIVDRNTVRLRVFERGAGETLACGTGACAAVVAGIRR
GLLDSPVKVHTHGGDLTIAWQGAGQPVQMTGPATTVFEGTIDLSTLPA

Sequences:

>Translated_288_residues
MKLQFTKMHGAGNDFIVLDGIHQKLDLTDAQWRALANRHFGIGADQILIVEKSSRDDVDFRYRIVNADGGEVEHCGNGAR
CFVRFVTDRGMTDKQSVRVEVMNGVITLKLQDDGQVTVDMGEPELTPARVPFIADGLPTRAEAQDTLYGLEVNGRTEWIS
PVSMGNPHAVQIVDDVEQFPVLQDGPVIEHHKSFPNRVNAGFMQIVDRNTVRLRVFERGAGETLACGTGACAAVVAGIRR
GLLDSPVKVHTHGGDLTIAWQGAGQPVQMTGPATTVFEGTIDLSTLPA
>Mature_288_residues
MKLQFTKMHGAGNDFIVLDGIHQKLDLTDAQWRALANRHFGIGADQILIVEKSSRDDVDFRYRIVNADGGEVEHCGNGAR
CFVRFVTDRGMTDKQSVRVEVMNGVITLKLQDDGQVTVDMGEPELTPARVPFIADGLPTRAEAQDTLYGLEVNGRTEWIS
PVSMGNPHAVQIVDDVEQFPVLQDGPVIEHHKSFPNRVNAGFMQIVDRNTVRLRVFERGAGETLACGTGACAAVVAGIRR
GLLDSPVKVHTHGGDLTIAWQGAGQPVQMTGPATTVFEGTIDLSTLPA

Specific function: Biosynthesis of lysine from aspartate semialdehyde; sixth step. [C]

COG id: COG0253

COG function: function code E; Diaminopimelate epimerase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the diaminopimelate epimerase family

Homologues:

Organism=Escherichia coli, GI87082334, Length=281, Percent_Identity=51.2455516014235, Blast_Score=288, Evalue=2e-79,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DAPF_RALME (Q1LS37)

Other databases:

- EMBL:   CP000352
- RefSeq:   YP_582308.1
- ProteinModelPortal:   Q1LS37
- SMR:   Q1LS37
- STRING:   Q1LS37
- GeneID:   4036937
- GenomeReviews:   CP000352_GR
- KEGG:   rme:Rmet_0153
- eggNOG:   COG0253
- HOGENOM:   HBG399442
- OMA:   HTGIGFD
- PhylomeDB:   Q1LS37
- ProtClustDB:   PRK00450
- BioCyc:   RMET266264:RMET_0153-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00197
- InterPro:   IPR001653
- InterPro:   IPR018510
- TIGRFAMs:   TIGR00652

Pfam domain/function: PF01678 DAP_epimerase

EC number: =5.1.1.7

Molecular weight: Translated: 31295; Mature: 31295

Theoretical pI: Translated: 5.34; Mature: 5.34

Prosite motif: PS01326 DAP_EPIMERASE

Important sites: ACT_SITE 75-75 ACT_SITE 226-226

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLQFTKMHGAGNDFIVLDGIHQKLDLTDAQWRALANRHFGIGADQILIVEKSSRDDVDF
CCEEEEEECCCCCCEEEECCCCCCCCCCHHHHHHHHCCCCCCCCCEEEEEECCCCCCCEE
RYRIVNADGGEVEHCGNGARCFVRFVTDRGMTDKQSVRVEVMNGVITLKLQDDGQVTVDM
EEEEEECCCCCEEECCCCCEEEEEEEECCCCCCCHHEEEEEECCEEEEEECCCCEEEEEC
GEPELTPARVPFIADGLPTRAEAQDTLYGLEVNGRTEWISPVSMGNPHAVQIVDDVEQFP
CCCCCCCCCCCEEECCCCCCCCCCCEEEEEEECCCEEEECCCCCCCCEEEEEEECHHHCC
VLQDGPVIEHHKSFPNRVNAGFMQIVDRNTVRLRVFERGAGETLACGTGACAAVVAGIRR
CCCCCCCCHHHCCCCCCCCCCEEEEECCCEEEEEEEECCCCCEEEECCCHHHHHHHHHHH
GLLDSPVKVHTHGGDLTIAWQGAGQPVQMTGPATTVFEGTIDLSTLPA
HHCCCCEEEEECCCCEEEEECCCCCCEEEECCCEEEEECEEEEEECCC
>Mature Secondary Structure
MKLQFTKMHGAGNDFIVLDGIHQKLDLTDAQWRALANRHFGIGADQILIVEKSSRDDVDF
CCEEEEEECCCCCCEEEECCCCCCCCCCHHHHHHHHCCCCCCCCCEEEEEECCCCCCCEE
RYRIVNADGGEVEHCGNGARCFVRFVTDRGMTDKQSVRVEVMNGVITLKLQDDGQVTVDM
EEEEEECCCCCEEECCCCCEEEEEEEECCCCCCCHHEEEEEECCEEEEEECCCCEEEEEC
GEPELTPARVPFIADGLPTRAEAQDTLYGLEVNGRTEWISPVSMGNPHAVQIVDDVEQFP
CCCCCCCCCCCEEECCCCCCCCCCCEEEEEEECCCEEEECCCCCCCCEEEEEEECHHHCC
VLQDGPVIEHHKSFPNRVNAGFMQIVDRNTVRLRVFERGAGETLACGTGACAAVVAGIRR
CCCCCCCCHHHCCCCCCCCCCEEEEECCCEEEEEEEECCCCCEEEECCCHHHHHHHHHHH
GLLDSPVKVHTHGGDLTIAWQGAGQPVQMTGPATTVFEGTIDLSTLPA
HHCCCCEEEEECCCCEEEEECCCCCCEEEECCCEEEEECEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA