Click here to switch to the map view.

The map label for this gene is lpxL [H]

Identifier: 94309099

GI number: 94309099

Start: 158440

End: 159333

Strand: Reverse

Name: lpxL [H]

Synonym: Rmet_0154

Alternate gene names: 94309099

Gene position: 159333-158440 (Counterclockwise)

Preceding gene: 94309100

Following gene: 94309098

Centisome position: 4.06

GC content: 63.53

Gene sequence:

>894_bases
ATGAGCCGGATCTTCACGTGGCTCGGCATCGGTCTGCTGACGGTGCTGGGCAAACTCCCCTACCCGTTCGTGGCCCGCTT
CGGCGAGGCGCTGGGCAGCATTCTGTTCATGATCCCAAATCCGCGCCGCGAAGTGGTGCGGGCCAACCTTCGGCTGTGCT
TCCCGGACAAGACCGATGCGGAAATCGAGGCGATGTCGCGCGAGACCTACCGCAAGGTCTTCCGCAGCTTTGCCGAGGGC
GGCATCTTCTGGACATGCAACGAGAAAAAGATGCGCCAGCTGGTGCAGATCAACGACCAGGGCAACCTGCCCTCGCTCGA
CGGCTCGCCCCATATCCTGGTGACACTGCACCTGTCCGGCCTGGAAGCTGGTGCGATTCGCCTGACGATGCACCTGCGCG
ACACCGTGGGCCGTTCGGGCGCCTCGCTCTACACGGTGCAGAAGAACGCGCTGTTCGATGACTTCCTGAAGGTCGCGCGC
GGCCGCTTTGGCGCCAACATGATTTCGCGCAACGACAGCGTGCGCAACATCCTCCGCGTGCTGAAGAAAGGCGAGGCCCT
GCAGCTGATCTCCGACATGGACTTCGGCGAGCGCGACTCCGAGTTCGTGCCGTTCTTCGGCGTGGAGGCGCTGACCCTGA
CATCGGTCTCCCGGCTGGCCCGGCTTGCGGGCGCCAAGGTCGTACCCATCTACACCGAGCAGTTGCCAGACTACAAGGGG
TATGTACTGCACATCCTGCCGCCTTGGGACGACTACCCCGGCGAAAGCGTCACCGACGACACACGGCGCATGAACGCGTT
CTTCGAGGATGCCATCCGTCCGCGCGTGCCCGACTACTACTGGGTGCACAAACGCTTCAAGCACCGCCCGCCCGGCGAAC
CTGACATCTACTGA

Upstream 100 bases:

>100_bases
ACAAGTGTCCGACCGGCACGGAATTGCCGCCGGACACGTTCGCCACCACACAGGCCACTACACGTGCCACTACACAGGCC
ACTACCAACAACAACATTCA

Downstream 100 bases:

>100_bases
TTACAATTCCGGACATGAAACTCCAGTTCACCAAGATGCACGGCGCCGGCAACGATTTCATCGTGCTGGACGGCATTCAC
CAGAAGCTCGACCTGACCGA

Product: lipid A biosynthesis lauroyl acyltransferase

Products: NA

Alternate protein names: Heat shock protein B [H]

Number of amino acids: Translated: 297; Mature: 296

Protein sequence:

>297_residues
MSRIFTWLGIGLLTVLGKLPYPFVARFGEALGSILFMIPNPRREVVRANLRLCFPDKTDAEIEAMSRETYRKVFRSFAEG
GIFWTCNEKKMRQLVQINDQGNLPSLDGSPHILVTLHLSGLEAGAIRLTMHLRDTVGRSGASLYTVQKNALFDDFLKVAR
GRFGANMISRNDSVRNILRVLKKGEALQLISDMDFGERDSEFVPFFGVEALTLTSVSRLARLAGAKVVPIYTEQLPDYKG
YVLHILPPWDDYPGESVTDDTRRMNAFFEDAIRPRVPDYYWVHKRFKHRPPGEPDIY

Sequences:

>Translated_297_residues
MSRIFTWLGIGLLTVLGKLPYPFVARFGEALGSILFMIPNPRREVVRANLRLCFPDKTDAEIEAMSRETYRKVFRSFAEG
GIFWTCNEKKMRQLVQINDQGNLPSLDGSPHILVTLHLSGLEAGAIRLTMHLRDTVGRSGASLYTVQKNALFDDFLKVAR
GRFGANMISRNDSVRNILRVLKKGEALQLISDMDFGERDSEFVPFFGVEALTLTSVSRLARLAGAKVVPIYTEQLPDYKG
YVLHILPPWDDYPGESVTDDTRRMNAFFEDAIRPRVPDYYWVHKRFKHRPPGEPDIY
>Mature_296_residues
SRIFTWLGIGLLTVLGKLPYPFVARFGEALGSILFMIPNPRREVVRANLRLCFPDKTDAEIEAMSRETYRKVFRSFAEGG
IFWTCNEKKMRQLVQINDQGNLPSLDGSPHILVTLHLSGLEAGAIRLTMHLRDTVGRSGASLYTVQKNALFDDFLKVARG
RFGANMISRNDSVRNILRVLKKGEALQLISDMDFGERDSEFVPFFGVEALTLTSVSRLARLAGAKVVPIYTEQLPDYKGY
VLHILPPWDDYPGESVTDDTRRMNAFFEDAIRPRVPDYYWVHKRFKHRPPGEPDIY

Specific function: Acylates the intermediate (KDO)2-lipid IVA to form (KDO)2-(lauroyl)-lipid IVA. Has 10 fold selectivity for lauroyl- ACP over myristoyl-ACP [H]

COG id: COG1560

COG function: function code M; Lauroyl/myristoyl acyltransferase

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the htrB/msbB family [H]

Homologues:

Organism=Escherichia coli, GI1787292, Length=296, Percent_Identity=33.1081081081081, Blast_Score=160, Evalue=7e-41,
Organism=Escherichia coli, GI87082094, Length=299, Percent_Identity=31.438127090301, Blast_Score=149, Evalue=3e-37,
Organism=Escherichia coli, GI1788161, Length=300, Percent_Identity=27.6666666666667, Blast_Score=109, Evalue=2e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004960
- InterPro:   IPR011920 [H]

Pfam domain/function: PF03279 Lip_A_acyltrans [H]

EC number: 2.3.1.-

Molecular weight: Translated: 33844; Mature: 33713

Theoretical pI: Translated: 9.44; Mature: 9.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRIFTWLGIGLLTVLGKLPYPFVARFGEALGSILFMIPNPRREVVRANLRLCFPDKTDA
CCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHEEEECCCCHHHHHHCCCEEECCCCCCH
EIEAMSRETYRKVFRSFAEGGIFWTCNEKKMRQLVQINDQGNLPSLDGSPHILVTLHLSG
HHHHHHHHHHHHHHHHHHCCCEEEEECHHHHHHHHHCCCCCCCCCCCCCCEEEEEEEECC
LEAGAIRLTMHLRDTVGRSGASLYTVQKNALFDDFLKVARGRFGANMISRNDSVRNILRV
CCCCEEEEEEEEHHHCCCCCCEEEEEEHHHHHHHHHHHHHCCCCCHHHHCCHHHHHHHHH
LKKGEALQLISDMDFGERDSEFVPFFGVEALTLTSVSRLARLAGAKVVPIYTEQLPDYKG
HHCCCCEEHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCEEEEEHHHHCCCCCC
YVLHILPPWDDYPGESVTDDTRRMNAFFEDAIRPRVPDYYWVHKRFKHRPPGEPDIY
EEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure 
SRIFTWLGIGLLTVLGKLPYPFVARFGEALGSILFMIPNPRREVVRANLRLCFPDKTDA
CHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHEEEECCCCHHHHHHCCCEEECCCCCCH
EIEAMSRETYRKVFRSFAEGGIFWTCNEKKMRQLVQINDQGNLPSLDGSPHILVTLHLSG
HHHHHHHHHHHHHHHHHHCCCEEEEECHHHHHHHHHCCCCCCCCCCCCCCEEEEEEEECC
LEAGAIRLTMHLRDTVGRSGASLYTVQKNALFDDFLKVARGRFGANMISRNDSVRNILRV
CCCCEEEEEEEEHHHCCCCCCEEEEEEHHHHHHHHHHHHHCCCCCHHHHCCHHHHHHHHH
LKKGEALQLISDMDFGERDSEFVPFFGVEALTLTSVSRLARLAGAKVVPIYTEQLPDYKG
HHCCCCEEHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCEEEEEHHHHCCCCCC
YVLHILPPWDDYPGESVTDDTRRMNAFFEDAIRPRVPDYYWVHKRFKHRPPGEPDIY
EEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Acyltransferases [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]