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The map label for this gene is hipO [H]

Identifier: 94308966

GI number: 94308966

Start: 24791

End: 26068

Strand: Direct

Name: hipO [H]

Synonym: Rmet_0021

Alternate gene names: 94308966

Gene position: 24791-26068 (Clockwise)

Preceding gene: 94308965

Following gene: 94308968

Centisome position: 0.63

GC content: 64.87

Gene sequence:

>1278_bases
ATGTCTGAACACGCCCCCGAAATCGAAGACCCCGACATCGACGCCCCTGAAGTCGCATCGATCCTCAAGTCTGTCTCCGT
CCGCCTCGCCAAGGATTCCCCCACCCGGTCCTTCTGCACCCTGCACGACACGCTCGACTCGCGTGACGAACTGCAGGACA
TCCGCCGCCATATCCACCAGCATCCCGAGCTTGCCTTCAACGAGGTCCACACGGCCGAACTCGTCGCCAGCAAGCTGGAG
AACTGGGGCTACACGGTCACACGTGGCGTGGGCGGTACTGGCGTGGTGGCCTCGCTGCGAGTGGGCGCGGGGCAACGCAG
CGTCGGCATTCGCGCCGACATGGATGCATTGCCGATCCACGAGCGGTCGGGCTTGCCCTATGCCAGCGTGCACGACGGCA
AGATGCACGCGTGTGGCCATGATGGCCACACCGCCGTGCTACTGGGTGCCGCGCGGCAACTGGCACGTACCCGGCAATTC
GATGGCACCGTGCACCTGATCTTCCAGCCGGCCGAGGAGATCGGGGCGGGCGGTGGCGCGGAACGGATGCTGGCCGACGG
CCTGTTCGAACGCTTTCCGTGCGATGCGATATTTGGCCTGCATAACCATCCGGGTGCCGAGGCCGGTACCTTCATGTTCC
GTTCGGGCCCGTTCATGGCCGCGTGCGACACCGTAGCGATCACCATCCGAGGGAAGGGGGGCCATGCCGCGCGGCCGCAT
CAGTCGATTGATCCGATCCTGGTCGCGGGCAGTCTGGTCATGGCGCTGCAGTCCATCGTGGCGCGCAACGTCGATCCGAA
TGAGACTGCCGTGGTGACCATCGGCACGCTGCATGCGGGCCACGCGCCCAATGTGATTCCAGATAGCGCGCGCATGGAAC
TCAGCGTGCGCTCATTCAGTGCGGATGTCCGGGCATCCATGGAAACGCGCATCAAGCAGCTTGCCACGTCACACGCCGAG
GGCTACGGCGCGACGGCCGATATCGAGTACGTGCGTGGCTATCCGGTGCTCGTCAACAGCGAAGCGGAGACCGAGTTCGC
ACGCCAGGTGGCCGAGGAACTGGTGGGCCCCGAACGTGCAATCGCGAATTTCCACCGCATCGCCGGCAGTGAGGACTTCG
CTTACTTCCTGCAGCAGCGGCCCGGGTGTTTCGTCCGGATGGGCAATGGGGTCAACCAGCCGCTGCTTCACAACGCCGGC
TACGACTTCAATGACGACAACCTGACCGTCGGGGCGGCTTACTGGACGCGGCTGGTCGAGCGTTACCTCGCCGGGTAG

Upstream 100 bases:

>100_bases
CGTGATTCCGGGCGTTTTCGCACGAAATCAGCATTCCAGGTTTTCTTCCTTGGGGTGCGTGAAATACTGTTCATACATGT
CTGCCGTTTAGAGCACTGTC

Downstream 100 bases:

>100_bases
GACAGGGCGACAGGACTATTTGAAGAAGGCACTCGGCGGCACGCCGAACTGCCGGCGGAACATCGTCGCGAAGGCGCTCG
GGCTGCCATAGCCCATGTCG

Product: putative peptidase, M20D subfamily

Products: NA

Alternate protein names: Benzoylglycine amidohydrolase; Hippuricase [H]

Number of amino acids: Translated: 425; Mature: 424

Protein sequence:

>425_residues
MSEHAPEIEDPDIDAPEVASILKSVSVRLAKDSPTRSFCTLHDTLDSRDELQDIRRHIHQHPELAFNEVHTAELVASKLE
NWGYTVTRGVGGTGVVASLRVGAGQRSVGIRADMDALPIHERSGLPYASVHDGKMHACGHDGHTAVLLGAARQLARTRQF
DGTVHLIFQPAEEIGAGGGAERMLADGLFERFPCDAIFGLHNHPGAEAGTFMFRSGPFMAACDTVAITIRGKGGHAARPH
QSIDPILVAGSLVMALQSIVARNVDPNETAVVTIGTLHAGHAPNVIPDSARMELSVRSFSADVRASMETRIKQLATSHAE
GYGATADIEYVRGYPVLVNSEAETEFARQVAEELVGPERAIANFHRIAGSEDFAYFLQQRPGCFVRMGNGVNQPLLHNAG
YDFNDDNLTVGAAYWTRLVERYLAG

Sequences:

>Translated_425_residues
MSEHAPEIEDPDIDAPEVASILKSVSVRLAKDSPTRSFCTLHDTLDSRDELQDIRRHIHQHPELAFNEVHTAELVASKLE
NWGYTVTRGVGGTGVVASLRVGAGQRSVGIRADMDALPIHERSGLPYASVHDGKMHACGHDGHTAVLLGAARQLARTRQF
DGTVHLIFQPAEEIGAGGGAERMLADGLFERFPCDAIFGLHNHPGAEAGTFMFRSGPFMAACDTVAITIRGKGGHAARPH
QSIDPILVAGSLVMALQSIVARNVDPNETAVVTIGTLHAGHAPNVIPDSARMELSVRSFSADVRASMETRIKQLATSHAE
GYGATADIEYVRGYPVLVNSEAETEFARQVAEELVGPERAIANFHRIAGSEDFAYFLQQRPGCFVRMGNGVNQPLLHNAG
YDFNDDNLTVGAAYWTRLVERYLAG
>Mature_424_residues
SEHAPEIEDPDIDAPEVASILKSVSVRLAKDSPTRSFCTLHDTLDSRDELQDIRRHIHQHPELAFNEVHTAELVASKLEN
WGYTVTRGVGGTGVVASLRVGAGQRSVGIRADMDALPIHERSGLPYASVHDGKMHACGHDGHTAVLLGAARQLARTRQFD
GTVHLIFQPAEEIGAGGGAERMLADGLFERFPCDAIFGLHNHPGAEAGTFMFRSGPFMAACDTVAITIRGKGGHAARPHQ
SIDPILVAGSLVMALQSIVARNVDPNETAVVTIGTLHAGHAPNVIPDSARMELSVRSFSADVRASMETRIKQLATSHAEG
YGATADIEYVRGYPVLVNSEAETEFARQVAEELVGPERAIANFHRIAGSEDFAYFLQQRPGCFVRMGNGVNQPLLHNAGY
DFNDDNLTVGAAYWTRLVERYLAG

Specific function: Required But Not Essential For Aminobenzoyl-Glutamate Utilization. May Participate In Hydrolysis Of Aminobenzoyl- Glutamate To Aminobenzoate, Either Alone Or In Combination With Abgb. [C]

COG id: COG1473

COG function: function code R; Metal-dependent amidase/aminoacylase/carboxypeptidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20 family [H]

Homologues:

Organism=Escherichia coli, GI87081880, Length=417, Percent_Identity=28.2973621103118, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1787598, Length=205, Percent_Identity=30.7317073170732, Blast_Score=65, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017439
- InterPro:   IPR010168
- InterPro:   IPR002933
- InterPro:   IPR011650 [H]

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20 [H]

EC number: =3.5.1.32 [H]

Molecular weight: Translated: 45896; Mature: 45765

Theoretical pI: Translated: 6.05; Mature: 6.05

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEHAPEIEDPDIDAPEVASILKSVSVRLAKDSPTRSFCTLHDTLDSRDELQDIRRHIHQ
CCCCCCCCCCCCCCHHHHHHHHHHHHEEEECCCCCCCEEEHHHHHCCHHHHHHHHHHHHC
HPELAFNEVHTAELVASKLENWGYTVTRGVGGTGVVASLRVGAGQRSVGIRADMDALPIH
CCCHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCEEEEEECCCCCCCCCEECCCCCCCH
ERSGLPYASVHDGKMHACGHDGHTAVLLGAARQLARTRQFDGTVHLIFQPAEEIGAGGGA
HCCCCCEEEECCCCEEECCCCCCEEHHHHHHHHHHHHHCCCCEEEEEEECHHHHCCCCCH
ERMLADGLFERFPCDAIFGLHNHPGAEAGTFMFRSGPFMAACDTVAITIRGKGGHAARPH
HHHHHHHHHHHCCCHHEECCCCCCCCCCCEEEEECCCCEEEECEEEEEEECCCCCCCCCC
QSIDPILVAGSLVMALQSIVARNVDPNETAVVTIGTLHAGHAPNVIPDSARMELSVRSFS
CCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEEECCCCCCCCCCCCCEEEEHHHHH
ADVRASMETRIKQLATSHAEGYGATADIEYVRGYPVLVNSEAETEFARQVAEELVGPERA
HHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHCCCCEEECCCHHHHHHHHHHHHHCCHHHH
IANFHRIAGSEDFAYFLQQRPGCFVRMGNGVNQPLLHNAGYDFNDDNLTVGAAYWTRLVE
HHHHHHHCCCHHHHHHHHCCCCEEEEECCCCCCCHHHCCCCCCCCCCEEEHHHHHHHHHH
RYLAG
HHHCC
>Mature Secondary Structure 
SEHAPEIEDPDIDAPEVASILKSVSVRLAKDSPTRSFCTLHDTLDSRDELQDIRRHIHQ
CCCCCCCCCCCCCHHHHHHHHHHHHEEEECCCCCCCEEEHHHHHCCHHHHHHHHHHHHC
HPELAFNEVHTAELVASKLENWGYTVTRGVGGTGVVASLRVGAGQRSVGIRADMDALPIH
CCCHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCEEEEEECCCCCCCCCEECCCCCCCH
ERSGLPYASVHDGKMHACGHDGHTAVLLGAARQLARTRQFDGTVHLIFQPAEEIGAGGGA
HCCCCCEEEECCCCEEECCCCCCEEHHHHHHHHHHHHHCCCCEEEEEEECHHHHCCCCCH
ERMLADGLFERFPCDAIFGLHNHPGAEAGTFMFRSGPFMAACDTVAITIRGKGGHAARPH
HHHHHHHHHHHCCCHHEECCCCCCCCCCCEEEEECCCCEEEECEEEEEEECCCCCCCCCC
QSIDPILVAGSLVMALQSIVARNVDPNETAVVTIGTLHAGHAPNVIPDSARMELSVRSFS
CCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEEECCCCCCCCCCCCCEEEEHHHHH
ADVRASMETRIKQLATSHAEGYGATADIEYVRGYPVLVNSEAETEFARQVAEELVGPERA
HHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHCCCCEEECCCHHHHHHHHHHHHHCCHHHH
IANFHRIAGSEDFAYFLQQRPGCFVRMGNGVNQPLLHNAGYDFNDDNLTVGAAYWTRLVE
HHHHHHHCCCHHHHHHHHCCCCEEEEECCCCCCCHHHCCCCCCCCCCEEEHHHHHHHHHH
RYLAG
HHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7730270; 10688204 [H]