Click here to switch to the map view.

The map label for this gene is proS [H]

Identifier: 335055647

GI number: 335055647

Start: 3359007

End: 3360851

Strand: Reverse

Name: proS [H]

Synonym: NA

Alternate gene names: 335055647

Gene position: 3360851-3359007 (Counterclockwise)

Preceding gene: 94312034

Following gene: 94312030

Centisome position: 85.56

GC content: 64.55

Gene sequence:

>1845_bases
ATGGCGGGAGTGCTGGTTTTCCCCGACCGGAAGGGCAGTTTCCGGCGCGTCCGGCCCAATGGATGCCGCATCGCGGCGCG
GTCGGGATGCCTGATGGCGCCCGGCCTTACGAGAAAGCAACGGATGAAAGCCTCGCAATTCTTCATTTCCACCCTCAAGG
AAGCCCCCGCCGACGCGGAAATCGTGTCGCATAAACTGATGATGCGGGCCGGCATGATCAAGAAGCTCGGCGCCGGCATC
TATAACTACATGCCGGTGGGCCTGCGTGTGATCCGCAAGGTCGAGAACATTGTTCGCGAGGAAATGAACCGTGCTGGCGC
CGTGGAGCTGTCGATGCCGGTGATCCAGCCTGCCGAACTCTGGCAGGAGACTGGCCGCTGGGACAAGATGGGGCCCGAGC
TGTTGCGTCTGAAGGATCGGCACGAGCGCGATTTCGCGGTGCAGCCCACTTCCGAGGAAGTGGTCACGGACATCGCCCGG
ACCGAAATCCGCAGCTACAAGCAACTACCGGTCAACTTCTACCAGATCCAGACCAAGTTCCGCGACGAGCGCCGTCCGCG
CTTCGGCATCATGCGTGGCCGCGAGTTCACGATGAAGGACGCCTATTCGTTCGACCGTGACATCGACGGCCTGAAGGCGT
CGTACCAGAACATGTACGACGCGTATGTGCGCATCTTCCAGCGCTTTGGCCTGGAATTCCGCGCCGTGGCGGCCGACAAC
GGCGCCATCGGCGGTTCGGGCTCGCACGAGTTCCACGTGATCGCCGATACCGGCGAGGACGCCATCGTTTACTGTCCGAC
GTCCGACTACGCCGCCAATATGGAAGCGGCCGAGGCGCTGCCGCTGCTGGCAACGCGTGCCGCGCCGGCCGAGGAATTGA
AGAAAACTTCGACGCCCGAGAAGGCCAAGTGCGAGCACGTCGCCGAATTCCTGGGCATCCCGCTGGAACGCACGGTCAAG
TCGATCGTGCTGGCCACCGACAGCGACGCCGGGGCCCAGATCTGGCTGCTGCTGATCCGTGGCGATCACGAACTGAACGA
GGTGAAGGCGTCGAAGGTGCCGGGCCTGGCTGACTTCCGCTTTGCGACTGAGACCGAAATCGTCGATACCTTCGGCTCGC
CCCCGGGCTACCTCGGTCCGATCGGTCCGAAGAAGCCGGTCAAGGTGGTGGCGGATCGTACCGTCGCCAACATGAGCGAC
TTCTGCTGTGGTGCCAACGATCGCGACTACCACTACACCGGTGTGAACTGGGGCCGCGACCTGCCGGAACCGGCTGTGGC
GGACCTTCGCAACGTGGTGGCCGGCGACGCCTCGCCGGACGGCAAGGGCACGCTTGACATCTGCCGTGGCATCGAAGTGG
GCCACGTGTTCATGCTCGGCACGCGCTATTCGGAGTCGATGAACGCCACGTTCCTGGACGAGAACGGCAAGACGCAGCCG
ATGCAGATGGGTTGCTACGGCATCGGCGTGACGCGGATTCTGGGCGCCGCCATCGAGCAGAACTATGACGAGCGAGGCAT
CATCTGGCCGGCGGCGATCGCCCCGTTCGCGGTGGTGGTGTGCCCGGTTGGCTACGATCGCAACGAGGCCGTCAAGGCCG
AGGCCGATCGCATCCACGCCGAGCTGCTGGCTGCGGGCGTCGACGTGATTCTGGACGACCGTGGCGAGCGCCCGGGCGTG
ATGTTTGCCGACTGGGAACTGATTGGCGTGCCGCACCGTGTGGTGGTGGGTGACCGTGGCCTCAAGGAAGGCAAAGTCGA
GTACCAGGGCCGCCGTGACGCCCAGGCCACCCCGGTGGCGGTGGCGGATGTGGTTGCGCACGTGCGCGGCCAGCTGGCTG
CCTGA

Upstream 100 bases:
NA

Downstream 100 bases:
NA

Product: prolyl-tRNA synthetase

Products: NA

Alternate protein names: Proline--tRNA ligase; ProRS [H]

Number of amino acids: Translated: 614; Mature: 613

Protein sequence:
NA

Sequences:

>Translated_614_residues
MAGVLVFPDRKGSFRRVRPNGCRIAARSGCLMAPGLTRKQRMKASQFFISTLKEAPADAEIVSHKLMMRAGMIKKLGAGI
YNYMPVGLRVIRKVENIVREEMNRAGAVELSMPVIQPAELWQETGRWDKMGPELLRLKDRHERDFAVQPTSEEVVTDIAR
TEIRSYKQLPVNFYQIQTKFRDERRPRFGIMRGREFTMKDAYSFDRDIDGLKASYQNMYDAYVRIFQRFGLEFRAVAADN
GAIGGSGSHEFHVIADTGEDAIVYCPTSDYAANMEAAEALPLLATRAAPAEELKKTSTPEKAKCEHVAEFLGIPLERTVK
SIVLATDSDAGAQIWLLLIRGDHELNEVKASKVPGLADFRFATETEIVDTFGSPPGYLGPIGPKKPVKVVADRTVANMSD
FCCGANDRDYHYTGVNWGRDLPEPAVADLRNVVAGDASPDGKGTLDICRGIEVGHVFMLGTRYSESMNATFLDENGKTQP
MQMGCYGIGVTRILGAAIEQNYDERGIIWPAAIAPFAVVVCPVGYDRNEAVKAEADRIHAELLAAGVDVILDDRGERPGV
MFADWELIGVPHRVVVGDRGLKEGKVEYQGRRDAQATPVAVADVVAHVRGQLAA
>Mature_613_residues
AGVLVFPDRKGSFRRVRPNGCRIAARSGCLMAPGLTRKQRMKASQFFISTLKEAPADAEIVSHKLMMRAGMIKKLGAGIY
NYMPVGLRVIRKVENIVREEMNRAGAVELSMPVIQPAELWQETGRWDKMGPELLRLKDRHERDFAVQPTSEEVVTDIART
EIRSYKQLPVNFYQIQTKFRDERRPRFGIMRGREFTMKDAYSFDRDIDGLKASYQNMYDAYVRIFQRFGLEFRAVAADNG
AIGGSGSHEFHVIADTGEDAIVYCPTSDYAANMEAAEALPLLATRAAPAEELKKTSTPEKAKCEHVAEFLGIPLERTVKS
IVLATDSDAGAQIWLLLIRGDHELNEVKASKVPGLADFRFATETEIVDTFGSPPGYLGPIGPKKPVKVVADRTVANMSDF
CCGANDRDYHYTGVNWGRDLPEPAVADLRNVVAGDASPDGKGTLDICRGIEVGHVFMLGTRYSESMNATFLDENGKTQPM
QMGCYGIGVTRILGAAIEQNYDERGIIWPAAIAPFAVVVCPVGYDRNEAVKAEADRIHAELLAAGVDVILDDRGERPGVM
FADWELIGVPHRVVVGDRGLKEGKVEYQGRRDAQATPVAVADVVAHVRGQLAA

Specific function: Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction:proline is first activated by ATP to form Pro- AMP and then transferred to the acceptor end of tRNA(Pro). As ProRS can inadvertently accommodate and process non-cognate amino acids su

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI34303926, Length=231, Percent_Identity=44.1558441558442, Blast_Score=222, Evalue=8e-58,
Organism=Escherichia coli, GI1786392, Length=573, Percent_Identity=56.7190226876091, Blast_Score=686, Evalue=0.0,
Organism=Caenorhabditis elegans, GI115532348, Length=268, Percent_Identity=34.3283582089552, Blast_Score=173, Evalue=3e-43,
Organism=Caenorhabditis elegans, GI193203271, Length=87, Percent_Identity=35.632183908046, Blast_Score=82, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6320931, Length=550, Percent_Identity=29.4545454545455, Blast_Score=253, Evalue=7e-68,
Organism=Drosophila melanogaster, GI24656200, Length=218, Percent_Identity=38.9908256880734, Blast_Score=175, Evalue=7e-44,

Paralogues:

None

Copy number: 800 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR002316
- InterPro:   IPR004500
- InterPro:   IPR007214 [H]

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b; PF04073 YbaK [H]

EC number: =6.1.1.15 [H]

Molecular weight: Translated: 67699; Mature: 67568

Theoretical pI: Translated: 6.59; Mature: 6.59

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAGVLVFPDRKGSFRRVRPNGCRIAARSGCLMAPGLTRKQRMKASQFFISTLKEAPADAE
CCCEEEECCCCCCCEEECCCCCEEEECCCEEECCCCCHHHHHHHHHHHHHHHHHCCCCHH
IVSHKLMMRAGMIKKLGAGIYNYMPVGLRVIRKVENIVREEMNRAGAVELSMPVIQPAEL
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCHHHH
WQETGRWDKMGPELLRLKDRHERDFAVQPTSEEVVTDIARTEIRSYKQLPVNFYQIQTKF
HHHCCCCCCCCHHHHHHHHCCCCCCEECCCHHHHHHHHHHHHHHHHHCCCCEEEEHHHHH
RDERRPRFGIMRGREFTMKDAYSFDRDIDGLKASYQNMYDAYVRIFQRFGLEFRAVAADN
HHHCCCCCCCCCCCCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCC
GAIGGSGSHEFHVIADTGEDAIVYCPTSDYAANMEAAEALPLLATRAAPAEELKKTSTPE
CCCCCCCCCEEEEEEECCCCEEEEECCCCCCCCCHHHHHHHHHHHCCCCHHHHHHCCCCC
KAKCEHVAEFLGIPLERTVKSIVLATDSDAGAQIWLLLIRGDHELNEVKASKVPGLADFR
HHHHHHHHHHHCCCHHHHHHEEEEECCCCCCCEEEEEEEECCCHHHHHHHHCCCCCCCCE
FATETEIVDTFGSPPGYLGPIGPKKPVKVVADRTVANMSDFCCGANDRDYHYTGVNWGRD
ECCHHHHHHHCCCCCCCCCCCCCCCCCEEEECHHHHCCHHHHCCCCCCCEEECCCCCCCC
LPEPAVADLRNVVAGDASPDGKGTLDICRGIEVGHVFMLGTRYSESMNATFLDENGKTQP
CCCHHHHHHHHHHCCCCCCCCCCHHHHHCCCCCCEEEEEECCCCCCCCCEEECCCCCCCC
MQMGCYGIGVTRILGAAIEQNYDERGIIWPAAIAPFAVVVCPVGYDRNEAVKAEADRIHA
CCCCEEHHHHHHHHHHHHHCCCCCCCCCCHHHHHCEEEEEECCCCCCCCCHHHHHHHHHH
ELLAAGVDVILDDRGERPGVMFADWELIGVPHRVVVGDRGLKEGKVEYQGRRDAQATPVA
HHHHHCCCEEEECCCCCCCEEEECEEEECCCCEEEECCCCCCCCCEEECCCCCCCCCCHH
VADVVAHVRGQLAA
HHHHHHHHHHHCCC
>Mature Secondary Structure 
AGVLVFPDRKGSFRRVRPNGCRIAARSGCLMAPGLTRKQRMKASQFFISTLKEAPADAE
CCEEEECCCCCCCEEECCCCCEEEECCCEEECCCCCHHHHHHHHHHHHHHHHHCCCCHH
IVSHKLMMRAGMIKKLGAGIYNYMPVGLRVIRKVENIVREEMNRAGAVELSMPVIQPAEL
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCHHHH
WQETGRWDKMGPELLRLKDRHERDFAVQPTSEEVVTDIARTEIRSYKQLPVNFYQIQTKF
HHHCCCCCCCCHHHHHHHHCCCCCCEECCCHHHHHHHHHHHHHHHHHCCCCEEEEHHHHH
RDERRPRFGIMRGREFTMKDAYSFDRDIDGLKASYQNMYDAYVRIFQRFGLEFRAVAADN
HHHCCCCCCCCCCCCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCC
GAIGGSGSHEFHVIADTGEDAIVYCPTSDYAANMEAAEALPLLATRAAPAEELKKTSTPE
CCCCCCCCCEEEEEEECCCCEEEEECCCCCCCCCHHHHHHHHHHHCCCCHHHHHHCCCCC
KAKCEHVAEFLGIPLERTVKSIVLATDSDAGAQIWLLLIRGDHELNEVKASKVPGLADFR
HHHHHHHHHHHCCCHHHHHHEEEEECCCCCCCEEEEEEEECCCHHHHHHHHCCCCCCCCE
FATETEIVDTFGSPPGYLGPIGPKKPVKVVADRTVANMSDFCCGANDRDYHYTGVNWGRD
ECCHHHHHHHCCCCCCCCCCCCCCCCCEEEECHHHHCCHHHHCCCCCCCEEECCCCCCCC
LPEPAVADLRNVVAGDASPDGKGTLDICRGIEVGHVFMLGTRYSESMNATFLDENGKTQP
CCCHHHHHHHHHHCCCCCCCCCCHHHHHCCCCCCEEEEEECCCCCCCCCEEECCCCCCCC
MQMGCYGIGVTRILGAAIEQNYDERGIIWPAAIAPFAVVVCPVGYDRNEAVKAEADRIHA
CCCCEEHHHHHHHHHHHHHCCCCCCCCCCHHHHHCEEEEEECCCCCCCCCHHHHHHHHHH
ELLAAGVDVILDDRGERPGVMFADWELIGVPHRVVVGDRGLKEGKVEYQGRRDAQATPVA
HHHHHCCCEEEECCCCCCCEEEECEEEECCCCEEEECCCCCCCCCEEECCCCCCCCCCHH
VADVVAHVRGQLAA
HHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA