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The map label for this gene is proB [H]

Identifier: 94312034

GI number: 94312034

Start: 3362319

End: 3363437

Strand: Reverse

Name: proB [H]

Synonym: Rmet_3103

Alternate gene names: 94312034

Gene position: 3363437-3362319 (Counterclockwise)

Preceding gene: 94312035

Following gene: 335055647

Centisome position: 85.63

GC content: 64.7

Gene sequence:

>1119_bases
ATGCAATCGGTCATCGCCCAGGCAAAACGCATTGTCGTCAAAGTAGGTTCGAGTCTGGTTACCAACGACGGCAAGGGGCT
CGACCACGATGCCATCGCGCGCTGGGCAGCTCAGATCGCCAAGCTGCGTGGCACGGGCAAGGAAGTCGTGCTGGTTAGCT
CAGGCGCGATTGCCGAAGGGATGCAGCGACTGGGCTGGGCGCGCCGGCCGAAGGAAATCCACGAACTGCAGGCCGCTGCC
GCGGTGGGCCAGATGGGCTTGGCGCAGGTCTACGAAAGCCAGTTCGGCCGATATGGCATCCGCACCGCGCAGGTGCTGCT
GACGCACGCTGACCTGGCCGATCGCGAGCGCTATCTGAATGCCCGTTCCACGCTGCTGACGCTGCTGTCCCTCGGCGTGG
TGCCGATCATCAACGAGAACGACACGGTCGTCACCGACGAAATCAAGTTCGGCGACAACGATACGCTCGGCGCGCTGGTG
ACGAATTTGATCGAAGGCGACGCATTGGTGATCTTGACCGACCAGCGTGGGCTCTACACCGCTGATCCGCGCAAGGACCC
GAATGCCGAGTTCGTCCATGAAGCGCTGGCTGGCACGCCCGAACTCGAGGCCATGGCCGGTGGCGCGGGCTCGTCTATTG
GCCGAGGCGGCATGCTGACCAAGATCCTGGCGGCCAAGCGGGCGGCGAAGTCTGGTGCTCATACGACGATCGCCTCGGGT
CGCGAGGCCGATGTGCTGGGCAGGCTGGCTGCCGGCGAGGCCATCGGCACGCAACTGCTGGCGCCGACCGGTCGTCTGAC
GGCGCGCAAGCAGTGGATGGCGGATCACCTGCAGTTGCGCGGCCGCGTGATCATCGACGCGGGCGCTGTAGAGAAGCTCA
CCAGCGGTGGCAAGTCGCTGCTGCCGATTGGCGTAGTGGAAGTGCAGGGCGAGTTCGCCCGTGGCGAGGTCATTGCGTGC
GCCAGCCCGGAGGGGAAGGAAGTGGCGCGCGGCATCACCAACTATTCGAGCGCCGAAGCCCGTCTGATCGCACGCAAGCC
GTCATCGGAGATCGAGTCGGTGCTGGGCCATCTGAACGAGCCCGAGTTGATCCATCGCGACAACCTTGTGCTGGTCTGA

Upstream 100 bases:

>100_bases
GAGCCCGATATCCGCCTCGACGACCGCCTGCATAACGTCGATCGAGACGACAGCAAAGCGTAACGCACTACAACAAGGCA
CCACGGAGACAGGACCCGCC

Downstream 100 bases:

>100_bases
GCGTGAAACAGTTACCCACGAACCCATGAAAAACGGCGGGGCCCGCAAAGGCCCCGCCGTTTTTTTATGCTCGGACTACC
GTTCAGCGGCTGGGCGCGTA

Product: gamma-glutamyl kinase

Products: NA

Alternate protein names: Gamma-glutamyl kinase; GK [H]

Number of amino acids: Translated: 372; Mature: 372

Protein sequence:

>372_residues
MQSVIAQAKRIVVKVGSSLVTNDGKGLDHDAIARWAAQIAKLRGTGKEVVLVSSGAIAEGMQRLGWARRPKEIHELQAAA
AVGQMGLAQVYESQFGRYGIRTAQVLLTHADLADRERYLNARSTLLTLLSLGVVPIINENDTVVTDEIKFGDNDTLGALV
TNLIEGDALVILTDQRGLYTADPRKDPNAEFVHEALAGTPELEAMAGGAGSSIGRGGMLTKILAAKRAAKSGAHTTIASG
READVLGRLAAGEAIGTQLLAPTGRLTARKQWMADHLQLRGRVIIDAGAVEKLTSGGKSLLPIGVVEVQGEFARGEVIAC
ASPEGKEVARGITNYSSAEARLIARKPSSEIESVLGHLNEPELIHRDNLVLV

Sequences:

>Translated_372_residues
MQSVIAQAKRIVVKVGSSLVTNDGKGLDHDAIARWAAQIAKLRGTGKEVVLVSSGAIAEGMQRLGWARRPKEIHELQAAA
AVGQMGLAQVYESQFGRYGIRTAQVLLTHADLADRERYLNARSTLLTLLSLGVVPIINENDTVVTDEIKFGDNDTLGALV
TNLIEGDALVILTDQRGLYTADPRKDPNAEFVHEALAGTPELEAMAGGAGSSIGRGGMLTKILAAKRAAKSGAHTTIASG
READVLGRLAAGEAIGTQLLAPTGRLTARKQWMADHLQLRGRVIIDAGAVEKLTSGGKSLLPIGVVEVQGEFARGEVIAC
ASPEGKEVARGITNYSSAEARLIARKPSSEIESVLGHLNEPELIHRDNLVLV
>Mature_372_residues
MQSVIAQAKRIVVKVGSSLVTNDGKGLDHDAIARWAAQIAKLRGTGKEVVLVSSGAIAEGMQRLGWARRPKEIHELQAAA
AVGQMGLAQVYESQFGRYGIRTAQVLLTHADLADRERYLNARSTLLTLLSLGVVPIINENDTVVTDEIKFGDNDTLGALV
TNLIEGDALVILTDQRGLYTADPRKDPNAEFVHEALAGTPELEAMAGGAGSSIGRGGMLTKILAAKRAAKSGAHTTIASG
READVLGRLAAGEAIGTQLLAPTGRLTARKQWMADHLQLRGRVIIDAGAVEKLTSGGKSLLPIGVVEVQGEFARGEVIAC
ASPEGKEVARGITNYSSAEARLIARKPSSEIESVLGHLNEPELIHRDNLVLV

Specific function: Catalyzes the transfer of a phosphate group to glutamate to form glutamate 5-phosphate which rapidly cyclizes to 5- oxoproline [H]

COG id: COG0263

COG function: function code E; Glutamate 5-kinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain [H]

Homologues:

Organism=Homo sapiens, GI21361368, Length=310, Percent_Identity=33.2258064516129, Blast_Score=134, Evalue=1e-31,
Organism=Homo sapiens, GI62912457, Length=308, Percent_Identity=33.4415584415584, Blast_Score=134, Evalue=2e-31,
Organism=Escherichia coli, GI1786437, Length=366, Percent_Identity=42.896174863388, Blast_Score=283, Evalue=1e-77,
Organism=Caenorhabditis elegans, GI17569967, Length=276, Percent_Identity=34.7826086956522, Blast_Score=145, Evalue=3e-35,
Organism=Caenorhabditis elegans, GI17569969, Length=276, Percent_Identity=34.7826086956522, Blast_Score=145, Evalue=3e-35,
Organism=Saccharomyces cerevisiae, GI6320506, Length=420, Percent_Identity=34.7619047619048, Blast_Score=214, Evalue=3e-56,
Organism=Saccharomyces cerevisiae, GI6321822, Length=420, Percent_Identity=33.3333333333333, Blast_Score=196, Evalue=4e-51,
Organism=Drosophila melanogaster, GI21357643, Length=348, Percent_Identity=31.0344827586207, Blast_Score=142, Evalue=4e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001048
- InterPro:   IPR001057
- InterPro:   IPR011529
- InterPro:   IPR005715
- InterPro:   IPR019797
- InterPro:   IPR002478
- InterPro:   IPR015947 [H]

Pfam domain/function: PF00696 AA_kinase; PF01472 PUA [H]

EC number: =2.7.2.11 [H]

Molecular weight: Translated: 39345; Mature: 39345

Theoretical pI: Translated: 7.30; Mature: 7.30

Prosite motif: PS50890 PUA ; PS00902 GLUTAMATE_5_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQSVIAQAKRIVVKVGSSLVTNDGKGLDHDAIARWAAQIAKLRGTGKEVVLVSSGAIAEG
CCHHHHHHHHHHHHHCCHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEECCHHHHH
MQRLGWARRPKEIHELQAAAAVGQMGLAQVYESQFGRYGIRTAQVLLTHADLADRERYLN
HHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCHHHHHHHHH
ARSTLLTLLSLGVVPIINENDTVVTDEIKFGDNDTLGALVTNLIEGDALVILTDQRGLYT
HHHHHHHHHHHCCEEEECCCCEEEEEEEECCCCCHHHHHHHHHHCCCEEEEEECCCCCEE
ADPRKDPNAEFVHEALAGTPELEAMAGGAGSSIGRGGMLTKILAAKRAAKSGAHTTIASG
CCCCCCCCHHHHHHHHCCCCCHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEECCCC
READVLGRLAAGEAIGTQLLAPTGRLTARKQWMADHLQLRGRVIIDAGAVEKLTSGGKSL
CCHHHHHHHHCCHHHHHHHHCCCCCHHHHHHHHHHHHHHCCEEEEECCHHHHHHCCCCCC
LPIGVVEVQGEFARGEVIACASPEGKEVARGITNYSSAEARLIARKPSSEIESVLGHLNE
CEEEEEEECCCCCCCCEEEECCCCHHHHHHHHCCCCCCHHEEEECCCHHHHHHHHHCCCC
PELIHRDNLVLV
CCEEECCCEEEC
>Mature Secondary Structure
MQSVIAQAKRIVVKVGSSLVTNDGKGLDHDAIARWAAQIAKLRGTGKEVVLVSSGAIAEG
CCHHHHHHHHHHHHHCCHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEECCHHHHH
MQRLGWARRPKEIHELQAAAAVGQMGLAQVYESQFGRYGIRTAQVLLTHADLADRERYLN
HHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCHHHHHHHHH
ARSTLLTLLSLGVVPIINENDTVVTDEIKFGDNDTLGALVTNLIEGDALVILTDQRGLYT
HHHHHHHHHHHCCEEEECCCCEEEEEEEECCCCCHHHHHHHHHHCCCEEEEEECCCCCEE
ADPRKDPNAEFVHEALAGTPELEAMAGGAGSSIGRGGMLTKILAAKRAAKSGAHTTIASG
CCCCCCCCHHHHHHHHCCCCCHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEECCCC
READVLGRLAAGEAIGTQLLAPTGRLTARKQWMADHLQLRGRVIIDAGAVEKLTSGGKSL
CCHHHHHHHHCCHHHHHHHHCCCCCHHHHHHHHHHHHHHCCEEEEECCHHHHHHCCCCCC
LPIGVVEVQGEFARGEVIACASPEGKEVARGITNYSSAEARLIARKPSSEIESVLGHLNE
CEEEEEEECCCCCCCCEEEECCCCHHHHHHHHCCCCCCHHEEEECCCHHHHHHHHHCCCC
PELIHRDNLVLV
CCEEECCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA