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The map label for this gene is thiL [H]

Identifier: 93007218

GI number: 93007218

Start: 2942817

End: 2943851

Strand: Direct

Name: thiL [H]

Synonym: Pcryo_2394

Alternate gene names: 93007218

Gene position: 2942817-2943851 (Clockwise)

Preceding gene: 93007217

Following gene: 93007219

Centisome position: 96.17

GC content: 45.7

Gene sequence:

>1035_bases
ATGAATGAATTTGAGCTGATTGAGCGCATATTTTCACAGATGCAAGCCACGCAGTCATCATCTAACGCTGTCGAAAAGGG
CATCGGTGATGATGCGGCTGTGATGGCATTGCCACAAGGCGCGCGCTTAGTCAGCTGTATTGACACACTCGTTCAAGGTC
GTCACTTTAGTGCTGATTGGGAACAGGTGAATAAACTGGCATTTGCTATTGGTTATAAAGCGGTAGCCGTTAACGTCTCT
GATATAGCGGCTATGGGTGCTACCCCGCATAGCATTTTACTAGCATTAGCATTACCTCAGCGCCTTGCTAATAGTCAGTG
GCTGACTGAGTTTGCCAAAGGTTTATTTCATGCTTGCCAGTTATTTGGTGTGACTTTGATAGGCGGTGATACGACCCGTA
ATGATAGCTTAGTGTTAAGTGTCAGTGCCCAAGGGCTGATTGCCGCAGATACTCCCGCTGTCTATCGCTCAGGTGCGCAA
GTCGGTGATAAAATTTATGTGTCAGGTACACTTGGTGATGCCGCTTATGCCTTACAGCACCCTGATAATGCTATCGGTAT
TGAACTTGCGCATCGACTTCATATGCCAACACCACGTATTAGCTTAGGTGCAGCACTAGCACAAATCGGTGCAACGTCAA
TGATAGATATCTCTGATGGTCTTTATCAAGATCTTGGTCATATCTGCGAGCAAAGCGGCATCGCTATGAGACTTAATTTG
GAAGCTCTTCCTACCAGTAACCCTTTAGCTAGTATCGATGTATCTGAGCGGCTATTGTGTCAATTAACTGGTGGCGATGA
TTATGAGTTGGCTTTTACCTTACCTCCCCATATCGAGCCATCTTCTTGTACCGACGTTAATACGCCTGTGACTTACATTG
GTAATGTGATTGCATTGGCGAGTGATAGAAATACAGATAATGCGGCGCATACACGTCCTGAACTTTATTATCAAGATCAG
CCAGTGACACCCACCCATCCCTCACCGTTTATTACTTGGCCCAATCTTACGGGTTACCAACATTTTGCAGGTTAA

Upstream 100 bases:

>100_bases
AAGTTACAGCAGAGGTCGAATTACTTGACTCGAATAGCGCTAATTTTGATACAAAAAGTGCTGAATTAAATGATGCTGAT
GAAAAAAGCCAAGACTAGCC

Downstream 100 bases:

>100_bases
CCCATGACCGATCACGACCTAGTTAAGCCCGATATCCGTAAAGCCAATGATTGCCCTCCTCTGCCAGTCAACGCCACTAT
GATTGACCGCGCAGTTTATT

Product: thiamine-monophosphate kinase

Products: NA

Alternate protein names: Thiamine-phosphate kinase [H]

Number of amino acids: Translated: 344; Mature: 344

Protein sequence:

>344_residues
MNEFELIERIFSQMQATQSSSNAVEKGIGDDAAVMALPQGARLVSCIDTLVQGRHFSADWEQVNKLAFAIGYKAVAVNVS
DIAAMGATPHSILLALALPQRLANSQWLTEFAKGLFHACQLFGVTLIGGDTTRNDSLVLSVSAQGLIAADTPAVYRSGAQ
VGDKIYVSGTLGDAAYALQHPDNAIGIELAHRLHMPTPRISLGAALAQIGATSMIDISDGLYQDLGHICEQSGIAMRLNL
EALPTSNPLASIDVSERLLCQLTGGDDYELAFTLPPHIEPSSCTDVNTPVTYIGNVIALASDRNTDNAAHTRPELYYQDQ
PVTPTHPSPFITWPNLTGYQHFAG

Sequences:

>Translated_344_residues
MNEFELIERIFSQMQATQSSSNAVEKGIGDDAAVMALPQGARLVSCIDTLVQGRHFSADWEQVNKLAFAIGYKAVAVNVS
DIAAMGATPHSILLALALPQRLANSQWLTEFAKGLFHACQLFGVTLIGGDTTRNDSLVLSVSAQGLIAADTPAVYRSGAQ
VGDKIYVSGTLGDAAYALQHPDNAIGIELAHRLHMPTPRISLGAALAQIGATSMIDISDGLYQDLGHICEQSGIAMRLNL
EALPTSNPLASIDVSERLLCQLTGGDDYELAFTLPPHIEPSSCTDVNTPVTYIGNVIALASDRNTDNAAHTRPELYYQDQ
PVTPTHPSPFITWPNLTGYQHFAG
>Mature_344_residues
MNEFELIERIFSQMQATQSSSNAVEKGIGDDAAVMALPQGARLVSCIDTLVQGRHFSADWEQVNKLAFAIGYKAVAVNVS
DIAAMGATPHSILLALALPQRLANSQWLTEFAKGLFHACQLFGVTLIGGDTTRNDSLVLSVSAQGLIAADTPAVYRSGAQ
VGDKIYVSGTLGDAAYALQHPDNAIGIELAHRLHMPTPRISLGAALAQIGATSMIDISDGLYQDLGHICEQSGIAMRLNL
EALPTSNPLASIDVSERLLCQLTGGDDYELAFTLPPHIEPSSCTDVNTPVTYIGNVIALASDRNTDNAAHTRPELYYQDQ
PVTPTHPSPFITWPNLTGYQHFAG

Specific function: Thiamine biosynthesis. [C]

COG id: COG0611

COG function: function code H; Thiamine monophosphate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiamine-monophosphate kinase family [H]

Homologues:

Organism=Escherichia coli, GI1786619, Length=352, Percent_Identity=37.5, Blast_Score=177, Evalue=6e-46,
Organism=Escherichia coli, GI1788062, Length=224, Percent_Identity=25, Blast_Score=62, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000728
- InterPro:   IPR010918
- InterPro:   IPR016188
- InterPro:   IPR006283 [H]

Pfam domain/function: PF00586 AIRS; PF02769 AIRS_C [H]

EC number: =2.7.4.16 [H]

Molecular weight: Translated: 36755; Mature: 36755

Theoretical pI: Translated: 4.65; Mature: 4.65

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNEFELIERIFSQMQATQSSSNAVEKGIGDDAAVMALPQGARLVSCIDTLVQGRHFSADW
CCHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCEEEECCCCCHHHHHHHHHHCCCCCCCCH
EQVNKLAFAIGYKAVAVNVSDIAAMGATPHSILLALALPQRLANSQWLTEFAKGLFHACQ
HHHHHHHHHCCCEEEEEEHHHHHHCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
LFGVTLIGGDTTRNDSLVLSVSAQGLIAADTPAVYRSGAQVGDKIYVSGTLGDAAYALQH
HHCEEEECCCCCCCCCEEEEEECCCEEEECCHHHHHCCCCCCCEEEEEECCCCCCCEECC
PDNAIGIELAHRLHMPTPRISLGAALAQIGATSMIDISDGLYQDLGHICEQSGIAMRLNL
CCCCHHHHHHHHHCCCCCCHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHCCCEEEEEE
EALPTSNPLASIDVSERLLCQLTGGDDYELAFTLPPHIEPSSCTDVNTPVTYIGNVIALA
EECCCCCCCEECCCCCEEEEEECCCCCEEEEEECCCCCCCCCCCCCCCCHHHHCCEEEEE
SDRNTDNAAHTRPELYYQDQPVTPTHPSPFITWPNLTGYQHFAG
CCCCCCCCCCCCCCEEECCCCCCCCCCCCEEECCCCCCCHHCCC
>Mature Secondary Structure
MNEFELIERIFSQMQATQSSSNAVEKGIGDDAAVMALPQGARLVSCIDTLVQGRHFSADW
CCHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCEEEECCCCCHHHHHHHHHHCCCCCCCCH
EQVNKLAFAIGYKAVAVNVSDIAAMGATPHSILLALALPQRLANSQWLTEFAKGLFHACQ
HHHHHHHHHCCCEEEEEEHHHHHHCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
LFGVTLIGGDTTRNDSLVLSVSAQGLIAADTPAVYRSGAQVGDKIYVSGTLGDAAYALQH
HHCEEEECCCCCCCCCEEEEEECCCEEEECCHHHHHCCCCCCCEEEEEECCCCCCCEECC
PDNAIGIELAHRLHMPTPRISLGAALAQIGATSMIDISDGLYQDLGHICEQSGIAMRLNL
CCCCHHHHHHHHHCCCCCCHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHCCCEEEEEE
EALPTSNPLASIDVSERLLCQLTGGDDYELAFTLPPHIEPSSCTDVNTPVTYIGNVIALA
EECCCCCCCEECCCCCEEEEEECCCCCEEEEEECCCCCCCCCCCCCCCCHHHHCCEEEEE
SDRNTDNAAHTRPELYYQDQPVTPTHPSPFITWPNLTGYQHFAG
CCCCCCCCCCCCCCEEECCCCCCCCCCCCEEECCCCCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]