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The map label for this gene is hisF [H]

Identifier: 93007215

GI number: 93007215

Start: 2940013

End: 2940795

Strand: Direct

Name: hisF [H]

Synonym: Pcryo_2391

Alternate gene names: 93007215

Gene position: 2940013-2940795 (Clockwise)

Preceding gene: 93007211

Following gene: 93007216

Centisome position: 96.08

GC content: 48.15

Gene sequence:

>783_bases
ATGTTAGCAAAGCGTATCATCCCTTGTTTGGACGTTGATAATGGGCGCGTGGTCAAAGGCGTACAATTTGTCGATATCAA
AGATGCAGGTGACCCTGTCGAGGTAGCGAAACGCTACAATGAACAAGGCGCCGATGAGATTACTTTTTTGGACATTACTG
CGACCAATGACGAGCGTGACACCACTTACCATACGGTAGAACGTATGGCGGAAACGGTATTTGTGCCATTAACTGTCGGC
GGCGGCGTGCGTAAAATTGCCGATATTCGTAATTTGCTCAATGCAGGCGCGGATAAAGTAGCGATCAATTCTGCTGCGGT
ATTTACCCCTGAGTTCGTCGGTGAAGCCTCGCAGAAATTCGGCAATCAATGTATCGTCGTTGCTATCGATGCCAAGCGCG
TTGCTGATATCGAGGTTGATGGCATTATCATGCCGCGTTGGGAAATATTTACCCACGGCGGTCGTAAGCCAACGGGCATT
GATGCCGTCGCTTGGGCAAGCAAAATGGCTGAGCTTGGCGCCGGTGAATTATTGGTAACTTCTATGGATGGCGATGGCAC
CAAAAAAGGCTACGATTTGGCATTAATGAAGCAAATTACCAGTCGTGTAAATGTACCAGTTATCGCGTCAGGTGGCGTCG
GTAATTTACAGCATTTAGCCGAAGGCGTATTAGAAGGCGGCGTCGATGCCGTCCTTGCTGCCAGTATTTTTCACTTTGGT
GAGTATACCGTTCAAGAAGCCAAAGCATATATGGCAGCGCAAGGTATACAAATGCGTTTGTAA

Upstream 100 bases:

>100_bases
GGTTAAAAGCATAATAATCTTGCGAAAACCTGCCTGAGATGGCAGTGATTTTGCTAGACTAGCGCTTATAGAATGAATTG
ATTATAAGGTGAAAGACCCT

Downstream 100 bases:

>100_bases
GACGTTGGTAAAATACAAATATAGTAGAAAAAAATGTTATCATAGCGCTAACTGCTTATTATCATTTGGTTTTCGTTCAT
TTTACTGCCTATTTATCTGC

Product: imidazole glycerol phosphate synthase subunit HisF

Products: NA

Alternate protein names: IGP synthase cyclase subunit; IGP synthase subunit hisF; ImGP synthase subunit hisF; IGPS subunit hisF [H]

Number of amino acids: Translated: 260; Mature: 260

Protein sequence:

>260_residues
MLAKRIIPCLDVDNGRVVKGVQFVDIKDAGDPVEVAKRYNEQGADEITFLDITATNDERDTTYHTVERMAETVFVPLTVG
GGVRKIADIRNLLNAGADKVAINSAAVFTPEFVGEASQKFGNQCIVVAIDAKRVADIEVDGIIMPRWEIFTHGGRKPTGI
DAVAWASKMAELGAGELLVTSMDGDGTKKGYDLALMKQITSRVNVPVIASGGVGNLQHLAEGVLEGGVDAVLAASIFHFG
EYTVQEAKAYMAAQGIQMRL

Sequences:

>Translated_260_residues
MLAKRIIPCLDVDNGRVVKGVQFVDIKDAGDPVEVAKRYNEQGADEITFLDITATNDERDTTYHTVERMAETVFVPLTVG
GGVRKIADIRNLLNAGADKVAINSAAVFTPEFVGEASQKFGNQCIVVAIDAKRVADIEVDGIIMPRWEIFTHGGRKPTGI
DAVAWASKMAELGAGELLVTSMDGDGTKKGYDLALMKQITSRVNVPVIASGGVGNLQHLAEGVLEGGVDAVLAASIFHFG
EYTVQEAKAYMAAQGIQMRL
>Mature_260_residues
MLAKRIIPCLDVDNGRVVKGVQFVDIKDAGDPVEVAKRYNEQGADEITFLDITATNDERDTTYHTVERMAETVFVPLTVG
GGVRKIADIRNLLNAGADKVAINSAAVFTPEFVGEASQKFGNQCIVVAIDAKRVADIEVDGIIMPRWEIFTHGGRKPTGI
DAVAWASKMAELGAGELLVTSMDGDGTKKGYDLALMKQITSRVNVPVIASGGVGNLQHLAEGVLEGGVDAVLAASIFHFG
EYTVQEAKAYMAAQGIQMRL

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisF subunit catalyzes the cyclization activity that produces IGP and AICAR from PRFAR using the ammonia provided by the hisH subunit [H]

COG id: COG0107

COG function: function code E; Imidazoleglycerol-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hisA/hisF family [H]

Homologues:

Organism=Escherichia coli, GI1788336, Length=264, Percent_Identity=41.2878787878788, Blast_Score=209, Evalue=2e-55,
Organism=Escherichia coli, GI87082028, Length=249, Percent_Identity=29.718875502008, Blast_Score=91, Evalue=6e-20,
Organism=Saccharomyces cerevisiae, GI6319725, Length=314, Percent_Identity=34.7133757961783, Blast_Score=164, Evalue=1e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006062
- InterPro:   IPR004651
- InterPro:   IPR011060 [H]

Pfam domain/function: PF00977 His_biosynth [H]

EC number: 4.1.3.-

Molecular weight: Translated: 27822; Mature: 27822

Theoretical pI: Translated: 4.78; Mature: 4.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLAKRIIPCLDVDNGRVVKGVQFVDIKDAGDPVEVAKRYNEQGADEITFLDITATNDERD
CCHHHCCCEEECCCCCEEECEEEEEECCCCCHHHHHHHHCCCCCCEEEEEEEEECCCCCC
TTYHTVERMAETVFVPLTVGGGVRKIADIRNLLNAGADKVAINSAAVFTPEFVGEASQKF
HHHHHHHHHHHHEEEEEEECCCHHHHHHHHHHHHCCCCEEEECCEEEECHHHHHHHHHHH
GNQCIVVAIDAKRVADIEVDGIIMPRWEIFTHGGRKPTGIDAVAWASKMAELGAGELLVT
CCEEEEEEECCCEEEEEEECCEEECCCEEEECCCCCCCCCHHHHHHHHHHHCCCCCEEEE
SMDGDGTKKGYDLALMKQITSRVNVPVIASGGVGNLQHLAEGVLEGGVDAVLAASIFHFG
ECCCCCCCCCCHHHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHHCCHHHHHHHHHHHHC
EYTVQEAKAYMAAQGIQMRL
HHHHHHHHHHHHHCCCEECC
>Mature Secondary Structure
MLAKRIIPCLDVDNGRVVKGVQFVDIKDAGDPVEVAKRYNEQGADEITFLDITATNDERD
CCHHHCCCEEECCCCCEEECEEEEEECCCCCHHHHHHHHCCCCCCEEEEEEEEECCCCCC
TTYHTVERMAETVFVPLTVGGGVRKIADIRNLLNAGADKVAINSAAVFTPEFVGEASQKF
HHHHHHHHHHHHEEEEEEECCCHHHHHHHHHHHHCCCCEEEECCEEEECHHHHHHHHHHH
GNQCIVVAIDAKRVADIEVDGIIMPRWEIFTHGGRKPTGIDAVAWASKMAELGAGELLVT
CCEEEEEEECCCEEEEEEECCEEECCCEEEECCCCCCCCCHHHHHHHHHHHCCCCCEEEE
SMDGDGTKKGYDLALMKQITSRVNVPVIASGGVGNLQHLAEGVLEGGVDAVLAASIFHFG
ECCCCCCCCCCHHHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHHCCHHHHHHHHHHHHC
EYTVQEAKAYMAAQGIQMRL
HHHHHHHHHHHHHCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Lyases; Carbon-Nitrogen Lyases; Amidine-Lyases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA