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The map label for this gene is glnB [H]

Identifier: 93007170

GI number: 93007170

Start: 2878062

End: 2878400

Strand: Direct

Name: glnB [H]

Synonym: Pcryo_2346

Alternate gene names: 93007170

Gene position: 2878062-2878400 (Clockwise)

Preceding gene: 93007169

Following gene: 93007171

Centisome position: 94.06

GC content: 46.61

Gene sequence:

>339_bases
ATGAAGCTAATCACTGCGATCTTAAAACCGTTTAAGCTCGATGATGTGCGTGAAGCGCTTTCTGACATTGGTGTAAAAGG
CGTTACCGTCACTGAAGTCAAAGGTTTCGGTCGTCAAAAAGGTCATACTGAGCTCTATCGCGGCGCCGAGTACGTGGTGG
ATTTTTTGCCTAAAGTAAAAGTGGAAGTGGCGGTGATGGATGAGATGGTTGAGCCAGCTATTGAAGCTATCACGCGTATC
GCTAGTACCGGTAAAATCGGAGACGGTAAGATATTTGTGACCAATCTCGAGCAAGTCATTCGTATTCGTACGGGTGAGAC
AGGTCCTGACGCTATCTAA

Upstream 100 bases:

>100_bases
TTGGCACAATTCTTGAATCATTTAATATAAGCATGATCATAATATGTTGCATCAAATGGCATCTATTGACCTGCGACATC
ACCCATATAAGGATTTTTTT

Downstream 100 bases:

>100_bases
GCGCAAGACTTACTTATAAATATCTCTTTATAGGTCATACCGATCTCTGTATTGCATATATTCAAGGGGGAATTAACATG
CAAAATCAAATCTTTGAGCT

Product: nitrogen regulatory protein P-II (GlnB, GlnK)

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 112; Mature: 112

Protein sequence:

>112_residues
MKLITAILKPFKLDDVREALSDIGVKGVTVTEVKGFGRQKGHTELYRGAEYVVDFLPKVKVEVAVMDEMVEPAIEAITRI
ASTGKIGDGKIFVTNLEQVIRIRTGETGPDAI

Sequences:

>Translated_112_residues
MKLITAILKPFKLDDVREALSDIGVKGVTVTEVKGFGRQKGHTELYRGAEYVVDFLPKVKVEVAVMDEMVEPAIEAITRI
ASTGKIGDGKIFVTNLEQVIRIRTGETGPDAI
>Mature_112_residues
MKLITAILKPFKLDDVREALSDIGVKGVTVTEVKGFGRQKGHTELYRGAEYVVDFLPKVKVEVAVMDEMVEPAIEAITRI
ASTGKIGDGKIFVTNLEQVIRIRTGETGPDAI

Specific function: In nitrogen-limiting conditions, when the ratio of Gln to 2-ketoglutarate decreases, P-II is uridylylated to P-II-UMP. P- II-UMP allows the deadenylation of glutamine synthetase (GS), thus activating the enzyme. Conversely, in nitrogen excess P-II is deur

COG id: COG0347

COG function: function code E; Nitrogen regulatory protein PII

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the P(II) protein family [H]

Homologues:

Organism=Escherichia coli, GI1786655, Length=112, Percent_Identity=66.9642857142857, Blast_Score=159, Evalue=4e-41,
Organism=Escherichia coli, GI1788904, Length=112, Percent_Identity=69.6428571428571, Blast_Score=159, Evalue=5e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002187
- InterPro:   IPR011322
- InterPro:   IPR015867
- InterPro:   IPR017918
- InterPro:   IPR002332 [H]

Pfam domain/function: PF00543 P-II [H]

EC number: NA

Molecular weight: Translated: 12243; Mature: 12243

Theoretical pI: Translated: 5.93; Mature: 5.93

Prosite motif: PS00496 PII_GLNB_UMP ; PS00638 PII_GLNB_CTER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLITAILKPFKLDDVREALSDIGVKGVTVTEVKGFGRQKGHTELYRGAEYVVDFLPKVK
CHHHHHHHCCCCHHHHHHHHHHCCCCCCEEHHHCCCCCCCCHHHHHHHHHHHHHHHCHHH
VEVAVMDEMVEPAIEAITRIASTGKIGDGKIFVTNLEQVIRIRTGETGPDAI
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MKLITAILKPFKLDDVREALSDIGVKGVTVTEVKGFGRQKGHTELYRGAEYVVDFLPKVK
CHHHHHHHCCCCHHHHHHHHHHCCCCCCEEHHHCCCCCCCCHHHHHHHHHHHHHHHCHHH
VEVAVMDEMVEPAIEAITRIASTGKIGDGKIFVTNLEQVIRIRTGETGPDAI
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8931324; 11481430 [H]