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The map label for this gene is amtB [C]

Identifier: 93007171

GI number: 93007171

Start: 2878478

End: 2879740

Strand: Direct

Name: amtB [C]

Synonym: Pcryo_2347

Alternate gene names: 93007171

Gene position: 2878478-2879740 (Clockwise)

Preceding gene: 93007170

Following gene: 93007175

Centisome position: 94.07

GC content: 46.08

Gene sequence:

>1263_bases
ATGCAAAATCAAATCTTTGAGCTCCAGTACGCGCTAGATACGTTTTACTTTTTAATGTGTGGCGCGCTTGTCATGTGGAT
GGCTGCGGGCTTCACAATGCTAGAGGCAGGGCTGGTTCGCTCAAAAAATACTGTCGAAATCCTAACAAAAAATATCGCGC
TTTTTGCGACTGCCTGTACCATGTATATGATATGCGGTTATGCCATCATGTATGACGGCAATCTGTTTTTGAGTGGTATT
GCAGGCACCGAAAGTTTGGTCACTGATGCATTAGCTACCTCAGCTGAAAACGGCTTTAATGGCGATGCAGTTTACTCCAG
CGCGTCCGACTTTTTCTTTCAAGTTGTCTTTGTAGCCACCTGTATGTCTATCGTCTCAGGCGCGGTCGCTGAACGTATGA
AACTATGGGCATTCTTACTGTTTGCCGTGGTGATGACTGGTGTCATTTATCCATTAGAAGGCAGCTGGACATGGGGTGGC
AAGGAAGTCTTTGGCTTATATAGTCTAGGCGACTTGGGTTTCTCTGATTTTGCAGGCTCTGGCATCGTACATATGGCAGG
TGCGGCAGCGGCGTTATCAGGTGTATTACTCTTAGGAGCGCGCCTAGGTAAATATGGCGTTAAAGGCGAGATACGCGCTA
TTCCTGGTGCAAACTTACCACTGGCAGCGCTTGGTACCTTAATCTTATGGATGGGCTGGTTTGGCTTTAACGGTGGTTCT
GTATTAAAACTGGGTGATATCGCTAGCGCCAATTCTGTCGCTGTCGTGTTTTTGAATACCAATGCCGCCGCTGCTGGTGG
AGCGATTGGTGCGTTGCTCATCGCACGCCTTATATTTGGTAAAGCCGATTTAACCATGCTCTTAAATGGTGCATTGGCAG
GTTTGGTTGCCATCACTGCAGAACCTTTAACGCCGTCAGCACTACAAGCTACCTTATTTGGTATGACTGCTGGTGTCATC
GTTGTCTTATCGATTTTACTACTAGACAAAATAAAAATTGACGACCCGGTAGGCGCTATTTCTGTCCATGGTGTGGTGGG
TTTATTTGGTCTGCTTATTGTACCGCTGACCAATCCGGCAGCGTCATTGCTTGGTCAACTAGTAGGTGCTGCGACTATTT
TTGCATGGGTCTTTATAAGCAGCTTAATCGTTTGGAGCATTATCAAGTTTGTCATCGGTCTGCGTATCTCTGAAGAAGAA
GAATATCAAGGTGCTGATATTGCCGAATGTGGTATGGAAGCCTATCCAGAATTTATAAGATAG

Upstream 100 bases:

>100_bases
AGACAGGTCCTGACGCTATCTAAGCGCAAGACTTACTTATAAATATCTCTTTATAGGTCATACCGATCTCTGTATTGCAT
ATATTCAAGGGGGAATTAAC

Downstream 100 bases:

>100_bases
TGGATATATTAATAACTCTGCTTTAAGAATGCTGTAGTGCTGCTAGCAGGGATTTCAAAGTCTCTGCCACGCATTGCTCA
CAGTTTACGATGAACATCTA

Product: Rh-like protein/ammonium transporter

Products: NH3 [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 420; Mature: 420

Protein sequence:

>420_residues
MQNQIFELQYALDTFYFLMCGALVMWMAAGFTMLEAGLVRSKNTVEILTKNIALFATACTMYMICGYAIMYDGNLFLSGI
AGTESLVTDALATSAENGFNGDAVYSSASDFFFQVVFVATCMSIVSGAVAERMKLWAFLLFAVVMTGVIYPLEGSWTWGG
KEVFGLYSLGDLGFSDFAGSGIVHMAGAAAALSGVLLLGARLGKYGVKGEIRAIPGANLPLAALGTLILWMGWFGFNGGS
VLKLGDIASANSVAVVFLNTNAAAAGGAIGALLIARLIFGKADLTMLLNGALAGLVAITAEPLTPSALQATLFGMTAGVI
VVLSILLLDKIKIDDPVGAISVHGVVGLFGLLIVPLTNPAASLLGQLVGAATIFAWVFISSLIVWSIIKFVIGLRISEEE
EYQGADIAECGMEAYPEFIR

Sequences:

>Translated_420_residues
MQNQIFELQYALDTFYFLMCGALVMWMAAGFTMLEAGLVRSKNTVEILTKNIALFATACTMYMICGYAIMYDGNLFLSGI
AGTESLVTDALATSAENGFNGDAVYSSASDFFFQVVFVATCMSIVSGAVAERMKLWAFLLFAVVMTGVIYPLEGSWTWGG
KEVFGLYSLGDLGFSDFAGSGIVHMAGAAAALSGVLLLGARLGKYGVKGEIRAIPGANLPLAALGTLILWMGWFGFNGGS
VLKLGDIASANSVAVVFLNTNAAAAGGAIGALLIARLIFGKADLTMLLNGALAGLVAITAEPLTPSALQATLFGMTAGVI
VVLSILLLDKIKIDDPVGAISVHGVVGLFGLLIVPLTNPAASLLGQLVGAATIFAWVFISSLIVWSIIKFVIGLRISEEE
EYQGADIAECGMEAYPEFIR
>Mature_420_residues
MQNQIFELQYALDTFYFLMCGALVMWMAAGFTMLEAGLVRSKNTVEILTKNIALFATACTMYMICGYAIMYDGNLFLSGI
AGTESLVTDALATSAENGFNGDAVYSSASDFFFQVVFVATCMSIVSGAVAERMKLWAFLLFAVVMTGVIYPLEGSWTWGG
KEVFGLYSLGDLGFSDFAGSGIVHMAGAAAALSGVLLLGARLGKYGVKGEIRAIPGANLPLAALGTLILWMGWFGFNGGS
VLKLGDIASANSVAVVFLNTNAAAAGGAIGALLIARLIFGKADLTMLLNGALAGLVAITAEPLTPSALQATLFGMTAGVI
VVLSILLLDKIKIDDPVGAISVHGVVGLFGLLIVPLTNPAASLLGQLVGAATIFAWVFISSLIVWSIIKFVIGLRISEEE
EYQGADIAECGMEAYPEFIR

Specific function: Involved In The Uptake Of Ammonia (Probable). [C]

COG id: COG0004

COG function: function code P; Ammonia permease

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ammonium transporter (TC 2.A.49) family [H]

Homologues:

Organism=Escherichia coli, GI1786656, Length=420, Percent_Identity=30.7142857142857, Blast_Score=155, Evalue=4e-39,
Organism=Caenorhabditis elegans, GI17550180, Length=435, Percent_Identity=31.9540229885057, Blast_Score=173, Evalue=1e-43,
Organism=Caenorhabditis elegans, GI17550182, Length=437, Percent_Identity=32.0366132723112, Blast_Score=169, Evalue=2e-42,
Organism=Caenorhabditis elegans, GI32564042, Length=432, Percent_Identity=28.7037037037037, Blast_Score=143, Evalue=1e-34,
Organism=Caenorhabditis elegans, GI133931100, Length=447, Percent_Identity=27.7404921700224, Blast_Score=132, Evalue=3e-31,
Organism=Saccharomyces cerevisiae, GI6324187, Length=420, Percent_Identity=27.8571428571429, Blast_Score=103, Evalue=5e-23,
Organism=Saccharomyces cerevisiae, GI6325396, Length=458, Percent_Identity=27.0742358078603, Blast_Score=94, Evalue=3e-20,
Organism=Saccharomyces cerevisiae, GI6321559, Length=432, Percent_Identity=27.0833333333333, Blast_Score=91, Evalue=3e-19,
Organism=Drosophila melanogaster, GI161078314, Length=418, Percent_Identity=31.8181818181818, Blast_Score=156, Evalue=3e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001905
- InterPro:   IPR018047 [H]

Pfam domain/function: PF00909 Ammonium_transp [H]

EC number: NA

Molecular weight: Translated: 44014; Mature: 44014

Theoretical pI: Translated: 4.33; Mature: 4.33

Prosite motif: PS01219 AMMONIUM_TRANSP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQNQIFELQYALDTFYFLMCGALVMWMAAGFTMLEAGLVRSKNTVEILTKNIALFATACT
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
MYMICGYAIMYDGNLFLSGIAGTESLVTDALATSAENGFNGDAVYSSASDFFFQVVFVAT
HHHHHCCCEEECCCEEEEECCCHHHHHHHHHHHHCCCCCCCCHHHCCHHHHHHHHHHHHH
CMSIVSGAVAERMKLWAFLLFAVVMTGVIYPLEGSWTWGGKEVFGLYSLGDLGFSDFAGS
HHHHHHHHHHHHHHHHHHHHHHHHHHCEEEECCCCCCCCCHHHEEEEEECCCCCHHHCCC
GIVHMAGAAAALSGVLLLGARLGKYGVKGEIRAIPGANLPLAALGTLILWMGWFGFNGGS
CHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCCHHHHHHHHHHHHHHCCCCCC
VLKLGDIASANSVAVVFLNTNAAAAGGAIGALLIARLIFGKADLTMLLNGALAGLVAITA
EEEECCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCC
EPLTPSALQATLFGMTAGVIVVLSILLLDKIKIDDPVGAISVHGVVGLFGLLIVPLTNPA
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCHH
ASLLGQLVGAATIFAWVFISSLIVWSIIKFVIGLRISEEEEYQGADIAECGMEAYPEFIR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHCHHHHHHHCC
>Mature Secondary Structure
MQNQIFELQYALDTFYFLMCGALVMWMAAGFTMLEAGLVRSKNTVEILTKNIALFATACT
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
MYMICGYAIMYDGNLFLSGIAGTESLVTDALATSAENGFNGDAVYSSASDFFFQVVFVAT
HHHHHCCCEEECCCEEEEECCCHHHHHHHHHHHHCCCCCCCCHHHCCHHHHHHHHHHHHH
CMSIVSGAVAERMKLWAFLLFAVVMTGVIYPLEGSWTWGGKEVFGLYSLGDLGFSDFAGS
HHHHHHHHHHHHHHHHHHHHHHHHHHCEEEECCCCCCCCCHHHEEEEEECCCCCHHHCCC
GIVHMAGAAAALSGVLLLGARLGKYGVKGEIRAIPGANLPLAALGTLILWMGWFGFNGGS
CHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCCHHHHHHHHHHHHHHCCCCCC
VLKLGDIASANSVAVVFLNTNAAAAGGAIGALLIARLIFGKADLTMLLNGALAGLVAITA
EEEECCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCC
EPLTPSALQATLFGMTAGVIVVLSILLLDKIKIDDPVGAISVHGVVGLFGLLIVPLTNPA
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCHH
ASLLGQLVGAATIFAWVFISSLIVWSIIKFVIGLRISEEEEYQGADIAECGMEAYPEFIR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NH3 [Periplasm] [C]

Specific reaction: NH3 [Periplasm] = NH3 [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]