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The map label for this gene is pheS [H]

Identifier: 93007123

GI number: 93007123

Start: 2827730

End: 2828785

Strand: Reverse

Name: pheS [H]

Synonym: Pcryo_2299

Alternate gene names: 93007123

Gene position: 2828785-2827730 (Counterclockwise)

Preceding gene: 93007124

Following gene: 93007122

Centisome position: 92.45

GC content: 47.63

Gene sequence:

>1056_bases
ATGACTACCCCTGCTGCTACCACCGATTTGTCGGCGCTACCGACCTTGTCCACAGAGCTAAGCGATCTTAATGAAGCTCA
GCTAAATGAGTTGGCTAGTGCCGCTGAAGCCTTGATTCTACAAGTAACTGATGTGCGTGCGTTGCAAGATTTGCGGGTGC
AATTGACCGGTAAAAAGAGTCCCTTAACTGGCTGGTCAAAGCAGATGGGTAAACTCAGCAGCGACGATAAAAAAACCTAC
GGCGGCTGGTTGCATCAAGTCCGTAGCCGTATTCAAGATGCGTTGACAGAGCAGCAGCAGCAGCTAGAAGTGGCTGCACT
AAATGCCAAGCTTGCAAGCGAGAGCATTGATATCACCTTGCCTGCTCGCGGTGGTCAAAAAGGTCATTTGCATCCTGTGA
CCATGATTACCCAGCGTATGCAGCAGTATTTTATACAAGCGGGTTTTAATGTAGCCACTGGTCCTGAAGTTGAGAGCGAT
TACTATAACTTTGAAGCGCTTAATATTCCGTTGCATCATCCAGCACGGGCGATGCACGATACCTTCTATTTTGATGCGCA
TTATTTACTGCGTACGCATACCAGTCCCGTGCAAATTCGCACCATGGAAAAGAATGAGCCGCCGATTCGTATTATTTGTC
CCGGTCGCGTCTATCGTAATGACTCGGACCAAACCCATTCGCCGATGTTCCATCAGCTAGAAGGTTTAATGGTTACCGAG
TCGAGCACTTTTGCTGAGCTAAAAGGCTTGATTAGTGAGTTCTTGGAAGCGTTTTTTGCCAAAGAGCTAACCGTACGTTT
CCGCCCGTCATTTTTCCCATTTACCGAGCCGTCAGCTGAAGTTGATATCCTCGATGATAATGGTAAATGGCTTGAGGTCA
TGGGCTGCGGTATGGTACATCCACAAGTGCTGACCAACTGCGGTATTGATGCCGAAAAATATACTGGTTTTGCCTTTGGT
ATGGGTATTGAGCGCTTTGCGATGCTGTACTACGGTATCGATGATTTGCGCCTGTTTTTCCAAAACGACGTACGCTTCTT
AAAGCAGTTTGGCTAG

Upstream 100 bases:

>100_bases
GTCACATGAATGGGTAAATGGTTTGCAATATTTGGTCAAATCCCTACAATTACCTATCACTATTATTAATAGTCCGTTTA
AATCAGGAAGAATGCGTCTT

Downstream 100 bases:

>100_bases
AGTGCTCTTTATTTATATAAAAAGTGCTCCTTGTTAATATAACAAGTTAATGTGCCAACCTTTACTTCAAAATATTTATT
ATAAAATTCTGATCCGAGAT

Product: phenylalanyl-tRNA synthetase subunit alpha

Products: NA

Alternate protein names: Phenylalanine--tRNA ligase alpha chain; PheRS [H]

Number of amino acids: Translated: 351; Mature: 350

Protein sequence:

>351_residues
MTTPAATTDLSALPTLSTELSDLNEAQLNELASAAEALILQVTDVRALQDLRVQLTGKKSPLTGWSKQMGKLSSDDKKTY
GGWLHQVRSRIQDALTEQQQQLEVAALNAKLASESIDITLPARGGQKGHLHPVTMITQRMQQYFIQAGFNVATGPEVESD
YYNFEALNIPLHHPARAMHDTFYFDAHYLLRTHTSPVQIRTMEKNEPPIRIICPGRVYRNDSDQTHSPMFHQLEGLMVTE
SSTFAELKGLISEFLEAFFAKELTVRFRPSFFPFTEPSAEVDILDDNGKWLEVMGCGMVHPQVLTNCGIDAEKYTGFAFG
MGIERFAMLYYGIDDLRLFFQNDVRFLKQFG

Sequences:

>Translated_351_residues
MTTPAATTDLSALPTLSTELSDLNEAQLNELASAAEALILQVTDVRALQDLRVQLTGKKSPLTGWSKQMGKLSSDDKKTY
GGWLHQVRSRIQDALTEQQQQLEVAALNAKLASESIDITLPARGGQKGHLHPVTMITQRMQQYFIQAGFNVATGPEVESD
YYNFEALNIPLHHPARAMHDTFYFDAHYLLRTHTSPVQIRTMEKNEPPIRIICPGRVYRNDSDQTHSPMFHQLEGLMVTE
SSTFAELKGLISEFLEAFFAKELTVRFRPSFFPFTEPSAEVDILDDNGKWLEVMGCGMVHPQVLTNCGIDAEKYTGFAFG
MGIERFAMLYYGIDDLRLFFQNDVRFLKQFG
>Mature_350_residues
TTPAATTDLSALPTLSTELSDLNEAQLNELASAAEALILQVTDVRALQDLRVQLTGKKSPLTGWSKQMGKLSSDDKKTYG
GWLHQVRSRIQDALTEQQQQLEVAALNAKLASESIDITLPARGGQKGHLHPVTMITQRMQQYFIQAGFNVATGPEVESDY
YNFEALNIPLHHPARAMHDTFYFDAHYLLRTHTSPVQIRTMEKNEPPIRIICPGRVYRNDSDQTHSPMFHQLEGLMVTES
STFAELKGLISEFLEAFFAKELTVRFRPSFFPFTEPSAEVDILDDNGKWLEVMGCGMVHPQVLTNCGIDAEKYTGFAFGM
GIERFAMLYYGIDDLRLFFQNDVRFLKQFG

Specific function: Unknown

COG id: COG0016

COG function: function code J; Phenylalanyl-tRNA synthetase alpha subunit

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. Phe-tRNA synthetase alpha chain type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI5729820, Length=265, Percent_Identity=32.0754716981132, Blast_Score=140, Evalue=2e-33,
Organism=Homo sapiens, GI4758340, Length=264, Percent_Identity=30.6818181818182, Blast_Score=124, Evalue=1e-28,
Organism=Escherichia coli, GI1788007, Length=325, Percent_Identity=57.8461538461538, Blast_Score=408, Evalue=1e-115,
Organism=Caenorhabditis elegans, GI32566635, Length=273, Percent_Identity=31.8681318681319, Blast_Score=134, Evalue=5e-32,
Organism=Caenorhabditis elegans, GI32563657, Length=263, Percent_Identity=31.5589353612167, Blast_Score=130, Evalue=7e-31,
Organism=Caenorhabditis elegans, GI17508957, Length=263, Percent_Identity=31.5589353612167, Blast_Score=130, Evalue=8e-31,
Organism=Saccharomyces cerevisiae, GI6321087, Length=369, Percent_Identity=24.6612466124661, Blast_Score=119, Evalue=5e-28,
Organism=Saccharomyces cerevisiae, GI6325304, Length=91, Percent_Identity=42.8571428571429, Blast_Score=88, Evalue=2e-18,
Organism=Drosophila melanogaster, GI17137424, Length=289, Percent_Identity=30.1038062283737, Blast_Score=126, Evalue=2e-29,
Organism=Drosophila melanogaster, GI18858079, Length=259, Percent_Identity=30.8880308880309, Blast_Score=119, Evalue=4e-27,

Paralogues:

None

Copy number: 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006195
- InterPro:   IPR004529
- InterPro:   IPR004188
- InterPro:   IPR022911
- InterPro:   IPR002319
- InterPro:   IPR010978 [H]

Pfam domain/function: PF02912 Phe_tRNA-synt_N; PF01409 tRNA-synt_2d [H]

EC number: =6.1.1.20 [H]

Molecular weight: Translated: 39549; Mature: 39418

Theoretical pI: Translated: 5.38; Mature: 5.38

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTPAATTDLSALPTLSTELSDLNEAQLNELASAAEALILQVTDVRALQDLRVQLTGKKS
CCCCCCCCCCHHCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEECCCC
PLTGWSKQMGKLSSDDKKTYGGWLHQVRSRIQDALTEQQQQLEVAALNAKLASESIDITL
CCCCHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEE
PARGGQKGHLHPVTMITQRMQQYFIQAGFNVATGPEVESDYYNFEALNIPLHHPARAMHD
ECCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEECCCCCCHHHHHH
TFYFDAHYLLRTHTSPVQIRTMEKNEPPIRIICPGRVYRNDSDQTHSPMFHQLEGLMVTE
HHEEEEEEEEEECCCCEEEEEECCCCCCEEEEECCCEECCCCCCCCCCHHHHHCCEEEEC
SSTFAELKGLISEFLEAFFAKELTVRFRPSFFPFTEPSAEVDILDDNGKWLEVMGCGMVH
CCHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCCCEEEEECCCCCEEEEEECCCCC
PQVLTNCGIDAEKYTGFAFGMGIERFAMLYYGIDDLRLFFQNDVRFLKQFG
HHHHHHCCCCHHHHCCCHHHCCHHHHHHHHHCHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TTPAATTDLSALPTLSTELSDLNEAQLNELASAAEALILQVTDVRALQDLRVQLTGKKS
CCCCCCCCCHHCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEECCCC
PLTGWSKQMGKLSSDDKKTYGGWLHQVRSRIQDALTEQQQQLEVAALNAKLASESIDITL
CCCCHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEE
PARGGQKGHLHPVTMITQRMQQYFIQAGFNVATGPEVESDYYNFEALNIPLHHPARAMHD
ECCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEECCCCCCHHHHHH
TFYFDAHYLLRTHTSPVQIRTMEKNEPPIRIICPGRVYRNDSDQTHSPMFHQLEGLMVTE
HHEEEEEEEEEECCCCEEEEEECCCCCCEEEEECCCEECCCCCCCCCCHHHHHCCEEEEC
SSTFAELKGLISEFLEAFFAKELTVRFRPSFFPFTEPSAEVDILDDNGKWLEVMGCGMVH
CCHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCCCEEEEECCCCCEEEEEECCCCC
PQVLTNCGIDAEKYTGFAFGMGIERFAMLYYGIDDLRLFFQNDVRFLKQFG
HHHHHHCCCCHHHHCCCHHHCCHHHHHHHHHCHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA