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The map label for this gene is pheT [H]

Identifier: 93007122

GI number: 93007122

Start: 2825220

End: 2827625

Strand: Reverse

Name: pheT [H]

Synonym: Pcryo_2298

Alternate gene names: 93007122

Gene position: 2827625-2825220 (Counterclockwise)

Preceding gene: 93007123

Following gene: 93007121

Centisome position: 92.41

GC content: 45.43

Gene sequence:

>2406_bases
ATGAAAATTAGCGAACAGTGGCTACGTCAATGGGTAAACCCCAACAATAGCAGTGAGCAATTGGCTGAACAACTCACGAT
GGCAGGGCTAGAGATTGATGATCGCTTTGCAGTCGCCCGTGCCTTCAGTGGTGTGGTCGTTGGTGAAGTTATCAGCGTTG
AGCAGCATCCAGATGCCGATAAATTACGTGTCACACAGGTCAATATTGGTGCAGCTGAGCCGTTACAAATCGTTTGCGGT
GCACCCAATGTGACTATTGGTATGAAAGTGCCTGTTGCGACTGTTGGTGCAGTTTTGCCAAGTGATGACAAAAAAGGCTT
CACAATTAAAAATGGCAACCTACGCGGTGTCGATTCTAACGGTATGCTCTGTGGCGCTTCTGAAATTGATTTAACCGACA
GCATCGATGGTTTACTTGAGCTGCCAGCAGATGCGCCAATTGGTATGGATATCCGTGAATATCTGGGCTTAGACAATCAA
ATATTGGATATTTCTATCACCCCAAACCGTGGTGATTGTTTTAGCGTACGTGGTATTGCGCGCGAGATATCCGTCATTAA
CGATTTGCCGTTACAAATGCCAAATATCCCTGATAATATCGATTTCACTCAAAACGAAGTGATGCCAGCTGTAACAGTCA
GTGCTGTAGAAGCTTGTCCACGCTACCTTTTACAGTCGATAAGCAATATCGATCGTAGTATCGATACGCCAAAGTGGATG
CAAGATGCGCTGGTTCAGTCAGGACTACGCTCGCACAACTTTTTGGTTGATGTGACCAACTATGTCTTGATGGAATTGGG
TCAGCCGCTACATGCTTTTGATGCTGATACTATCAAGGGCGATATCGTTGTGCGTTTGGCACAGTCTGAAGAAACGATTA
CTTTGTTGAATGAGCAAACTATTACCTTAACCGGTGATGAATTGGTCATTGCTGATGATAAAGGCGCACTTGCACTTGCC
GGAATTATGGGCGGTCAACGCAGTAGTGTGACTGATAGCACCACCAATATTGTGTTAGAGAGCGCATTCTTTAATCCATT
GGCTATCGCTGCACGCGCACGCCGTTTTGGTCTGCATACTGATGCCTCACAACGCTTTGAGCGTGGGGTAGACTTTGAAT
TGCCAGCGCTTGCTTTAGCGCGTGCTGTTGATTTGATTACCAGTGTAACGAATTCTCAAGCGGGGCAAATAATCACAGTC
GAAAGTAGCAAGCATTTACCGGCTCGTGCACCTATTACACTGCCGATTACTAAAGTCCGTGATGTCATCGGTATTGAGAT
TGAGCCTGCAGTCATGGTACGTATCTTGACTCAATTAGGCTTTAAGGTAGAGCAGCAAGCAGATAGCCTGATTTGCATGC
CGCCATCGTATCGCTTTGACATGAGCATTAAAGAAGATTTGATCGAAGAGATTGCTCGTATCTATGGCTATGACAATATT
CCAAGCGTCTTGCCGCACCTGCAAGTCAGCATGGATTATGACGATACCGCAGATTTGACGCATGAGATGAAGCTTTCGCT
AGTCGATAATGGCTATATGGAAGCAATTAGCTTTAGCTTTAGTGACGCAAAAATAGAAGCCTTGCTTGATGATGAAGCAT
TGGGCGAAGTGTTGGCATTGGCCAATCCTATCTCTAGTGACTTAGCCGTGATGCGCCGTACTTTGCTATCAAGCTTATTG
CCTTGTGTACAATATAATTTAAATCGTCAGCAGCCGCGAGTGCGTTTCTTTGAAACAGGGCTTAGCTTTGTTGGTCAAAG
TATTAGCGAGTTGGTGCAAACGCCAAGTATTGCATTGGTAGCAGTCGGAGATATTTGGGACGAGCAAGCGTATCAAAACC
GTGCATTAGACTTTTATGATCTTAAGCATGATATTGAGCAGTTATTACCTGCACAAATGGATAGTGCGCGTATCCGCTAT
GAGCGTAGTGAGCTAGCTTTCTTGCATCCGGGTCAAAGTGCCAAGCTTTATATCGATGATCAGTATGTCGGTTGGTTGGG
TCAACTGCATCCTAATACCGCTAAACAGTTGGATCTGCCAGCTACGTGGGTTGCGCAGTTGTCATTGGCACCGTTACTAA
CGCTTGCTCGTGAACAGCATGCCATCACCACTCCAAGCAAATTCCCGCAAGTACGCCGCGATATTGCTATATTAGTAGAC
AGCGATATCAGCCTTCAAACACTAGAGTCAACTATACGTAAGGCATCAGGTGCACTCTTGACTGATCTTTGGCTGTTTGA
TGTATACCAAGGCGAAAAAGTGCCAGCAGGTCAACGCTCATTAGCATTTGCCTTGATATGGCAAGATAAAATGCAAACCT
TATCGGATGACGCTGTAAAAACGGTCACTGATAAAGTGGTACAAGCATTAACGGTTGAGCATTCAGCACAGCTACGTGAC
AGCTAA

Upstream 100 bases:

>100_bases
CTCTTTATTTATATAAAAAGTGCTCCTTGTTAATATAACAAGTTAATGTGCCAACCTTTACTTCAAAATATTTATTATAA
AATTCTGATCCGAGATACTT

Downstream 100 bases:

>100_bases
CCTCTAATCGAAAAAGACAAATACTCTATAGCCTCAATATAAATGAGGCTATGGATTTTTGCTATTTATAAAAATTATTA
ACTGATAGAAGATTTTATTC

Product: phenylalanyl-tRNA synthetase subunit beta

Products: NA

Alternate protein names: Phenylalanine--tRNA ligase beta chain; PheRS [H]

Number of amino acids: Translated: 801; Mature: 801

Protein sequence:

>801_residues
MKISEQWLRQWVNPNNSSEQLAEQLTMAGLEIDDRFAVARAFSGVVVGEVISVEQHPDADKLRVTQVNIGAAEPLQIVCG
APNVTIGMKVPVATVGAVLPSDDKKGFTIKNGNLRGVDSNGMLCGASEIDLTDSIDGLLELPADAPIGMDIREYLGLDNQ
ILDISITPNRGDCFSVRGIAREISVINDLPLQMPNIPDNIDFTQNEVMPAVTVSAVEACPRYLLQSISNIDRSIDTPKWM
QDALVQSGLRSHNFLVDVTNYVLMELGQPLHAFDADTIKGDIVVRLAQSEETITLLNEQTITLTGDELVIADDKGALALA
GIMGGQRSSVTDSTTNIVLESAFFNPLAIAARARRFGLHTDASQRFERGVDFELPALALARAVDLITSVTNSQAGQIITV
ESSKHLPARAPITLPITKVRDVIGIEIEPAVMVRILTQLGFKVEQQADSLICMPPSYRFDMSIKEDLIEEIARIYGYDNI
PSVLPHLQVSMDYDDTADLTHEMKLSLVDNGYMEAISFSFSDAKIEALLDDEALGEVLALANPISSDLAVMRRTLLSSLL
PCVQYNLNRQQPRVRFFETGLSFVGQSISELVQTPSIALVAVGDIWDEQAYQNRALDFYDLKHDIEQLLPAQMDSARIRY
ERSELAFLHPGQSAKLYIDDQYVGWLGQLHPNTAKQLDLPATWVAQLSLAPLLTLAREQHAITTPSKFPQVRRDIAILVD
SDISLQTLESTIRKASGALLTDLWLFDVYQGEKVPAGQRSLAFALIWQDKMQTLSDDAVKTVTDKVVQALTVEHSAQLRD
S

Sequences:

>Translated_801_residues
MKISEQWLRQWVNPNNSSEQLAEQLTMAGLEIDDRFAVARAFSGVVVGEVISVEQHPDADKLRVTQVNIGAAEPLQIVCG
APNVTIGMKVPVATVGAVLPSDDKKGFTIKNGNLRGVDSNGMLCGASEIDLTDSIDGLLELPADAPIGMDIREYLGLDNQ
ILDISITPNRGDCFSVRGIAREISVINDLPLQMPNIPDNIDFTQNEVMPAVTVSAVEACPRYLLQSISNIDRSIDTPKWM
QDALVQSGLRSHNFLVDVTNYVLMELGQPLHAFDADTIKGDIVVRLAQSEETITLLNEQTITLTGDELVIADDKGALALA
GIMGGQRSSVTDSTTNIVLESAFFNPLAIAARARRFGLHTDASQRFERGVDFELPALALARAVDLITSVTNSQAGQIITV
ESSKHLPARAPITLPITKVRDVIGIEIEPAVMVRILTQLGFKVEQQADSLICMPPSYRFDMSIKEDLIEEIARIYGYDNI
PSVLPHLQVSMDYDDTADLTHEMKLSLVDNGYMEAISFSFSDAKIEALLDDEALGEVLALANPISSDLAVMRRTLLSSLL
PCVQYNLNRQQPRVRFFETGLSFVGQSISELVQTPSIALVAVGDIWDEQAYQNRALDFYDLKHDIEQLLPAQMDSARIRY
ERSELAFLHPGQSAKLYIDDQYVGWLGQLHPNTAKQLDLPATWVAQLSLAPLLTLAREQHAITTPSKFPQVRRDIAILVD
SDISLQTLESTIRKASGALLTDLWLFDVYQGEKVPAGQRSLAFALIWQDKMQTLSDDAVKTVTDKVVQALTVEHSAQLRD
S
>Mature_801_residues
MKISEQWLRQWVNPNNSSEQLAEQLTMAGLEIDDRFAVARAFSGVVVGEVISVEQHPDADKLRVTQVNIGAAEPLQIVCG
APNVTIGMKVPVATVGAVLPSDDKKGFTIKNGNLRGVDSNGMLCGASEIDLTDSIDGLLELPADAPIGMDIREYLGLDNQ
ILDISITPNRGDCFSVRGIAREISVINDLPLQMPNIPDNIDFTQNEVMPAVTVSAVEACPRYLLQSISNIDRSIDTPKWM
QDALVQSGLRSHNFLVDVTNYVLMELGQPLHAFDADTIKGDIVVRLAQSEETITLLNEQTITLTGDELVIADDKGALALA
GIMGGQRSSVTDSTTNIVLESAFFNPLAIAARARRFGLHTDASQRFERGVDFELPALALARAVDLITSVTNSQAGQIITV
ESSKHLPARAPITLPITKVRDVIGIEIEPAVMVRILTQLGFKVEQQADSLICMPPSYRFDMSIKEDLIEEIARIYGYDNI
PSVLPHLQVSMDYDDTADLTHEMKLSLVDNGYMEAISFSFSDAKIEALLDDEALGEVLALANPISSDLAVMRRTLLSSLL
PCVQYNLNRQQPRVRFFETGLSFVGQSISELVQTPSIALVAVGDIWDEQAYQNRALDFYDLKHDIEQLLPAQMDSARIRY
ERSELAFLHPGQSAKLYIDDQYVGWLGQLHPNTAKQLDLPATWVAQLSLAPLLTLAREQHAITTPSKFPQVRRDIAILVD
SDISLQTLESTIRKASGALLTDLWLFDVYQGEKVPAGQRSLAFALIWQDKMQTLSDDAVKTVTDKVVQALTVEHSAQLRD
S

Specific function: Unknown

COG id: COG0072

COG function: function code J; Phenylalanyl-tRNA synthetase beta subunit

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 tRNA-binding domain [H]

Homologues:

Organism=Homo sapiens, GI124028525, Length=410, Percent_Identity=25.1219512195122, Blast_Score=81, Evalue=3e-15,
Organism=Escherichia coli, GI1788006, Length=809, Percent_Identity=45.4882571075402, Blast_Score=647, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17533403, Length=593, Percent_Identity=22.5969645868465, Blast_Score=72, Evalue=1e-12,
Organism=Drosophila melanogaster, GI21355211, Length=413, Percent_Identity=24.455205811138, Blast_Score=69, Evalue=1e-11,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005146
- InterPro:   IPR005147
- InterPro:   IPR009061
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004532
- InterPro:   IPR020825
- InterPro:   IPR005121
- InterPro:   IPR002547 [H]

Pfam domain/function: PF03483 B3_4; PF03484 B5; PF03147 FDX-ACB; PF01588 tRNA_bind [H]

EC number: =6.1.1.20 [H]

Molecular weight: Translated: 88053; Mature: 88053

Theoretical pI: Translated: 4.36; Mature: 4.36

Prosite motif: PS50886 TRBD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKISEQWLRQWVNPNNSSEQLAEQLTMAGLEIDDRFAVARAFSGVVVGEVISVEQHPDAD
CCCHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHCCHHHHHEEECCCCCCCC
KLRVTQVNIGAAEPLQIVCGAPNVTIGMKVPVATVGAVLPSDDKKGFTIKNGNLRGVDSN
CEEEEEEECCCCCCEEEEECCCCEEEEEECCHHHHHCCCCCCCCCCEEEECCEEEEECCC
GMLCGASEIDLTDSIDGLLELPADAPIGMDIREYLGLDNQILDISITPNRGDCFSVRGIA
CEEECCCCCCCCCCCCHHHCCCCCCCCCCCHHHHCCCCCEEEEEEECCCCCCEEEHHHHH
REISVINDLPLQMPNIPDNIDFTQNEVMPAVTVSAVEACPRYLLQSISNIDRSIDTPKWM
HHHHHHHCCCCCCCCCCCCCCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHH
QDALVQSGLRSHNFLVDVTNYVLMELGQPLHAFDADTIKGDIVVRLAQSEETITLLNEQT
HHHHHHCCCCCCCEEEEHHHHHHHHCCCCHHHCCCCCCCCEEEEEEECCCCEEEEECCCE
ITLTGDELVIADDKGALALAGIMGGQRSSVTDSTTNIVLESAFFNPLAIAARARRFGLHT
EEEECCEEEEECCCCCEEEEEECCCCCCCCCCCHHHEEEEHHCCCHHHHHHHHHHCCCCC
DASQRFERGVDFELPALALARAVDLITSVTNSQAGQIITVESSKHLPARAPITLPITKVR
CHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCEEEEHHHHH
DVIGIEIEPAVMVRILTQLGFKVEQQADSLICMPPSYRFDMSIKEDLIEEIARIYGYDNI
HHHCCCCCHHHHHHHHHHHCCEEHHCCCCEEECCCCCCCCCCHHHHHHHHHHHHCCCCCC
PSVLPHLQVSMDYDDTADLTHEMKLSLVDNGYMEAISFSFSDAKIEALLDDEALGEVLAL
HHHHCCEEEEECCCCCCCCHHHHEEEEECCCCCHHEEECCCCCEEEEEECHHHHHHHHHH
ANPISSDLAVMRRTLLSSLLPCVQYNLNRQQPRVRFFETGLSFVGQSISELVQTPSIALV
CCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEE
AVGDIWDEQAYQNRALDFYDLKHDIEQLLPAQMDSARIRYERSELAFLHPGQSAKLYIDD
EECCHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCHHEEEECCCEEEECCCCCCEEEECC
QYVGWLGQLHPNTAKQLDLPATWVAQLSLAPLLTLAREQHAITTPSKFPQVRRDIAILVD
HHHHHHHCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCEEEEEE
SDISLQTLESTIRKASGALLTDLWLFDVYQGEKVPAGQRSLAFALIWQDKMQTLSDDAVK
CCCCHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCEEEEEEEHHHHHHHHHHHHH
TVTDKVVQALTVEHSAQLRDS
HHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKISEQWLRQWVNPNNSSEQLAEQLTMAGLEIDDRFAVARAFSGVVVGEVISVEQHPDAD
CCCHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHCCHHHHHEEECCCCCCCC
KLRVTQVNIGAAEPLQIVCGAPNVTIGMKVPVATVGAVLPSDDKKGFTIKNGNLRGVDSN
CEEEEEEECCCCCCEEEEECCCCEEEEEECCHHHHHCCCCCCCCCCEEEECCEEEEECCC
GMLCGASEIDLTDSIDGLLELPADAPIGMDIREYLGLDNQILDISITPNRGDCFSVRGIA
CEEECCCCCCCCCCCCHHHCCCCCCCCCCCHHHHCCCCCEEEEEEECCCCCCEEEHHHHH
REISVINDLPLQMPNIPDNIDFTQNEVMPAVTVSAVEACPRYLLQSISNIDRSIDTPKWM
HHHHHHHCCCCCCCCCCCCCCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHH
QDALVQSGLRSHNFLVDVTNYVLMELGQPLHAFDADTIKGDIVVRLAQSEETITLLNEQT
HHHHHHCCCCCCCEEEEHHHHHHHHCCCCHHHCCCCCCCCEEEEEEECCCCEEEEECCCE
ITLTGDELVIADDKGALALAGIMGGQRSSVTDSTTNIVLESAFFNPLAIAARARRFGLHT
EEEECCEEEEECCCCCEEEEEECCCCCCCCCCCHHHEEEEHHCCCHHHHHHHHHHCCCCC
DASQRFERGVDFELPALALARAVDLITSVTNSQAGQIITVESSKHLPARAPITLPITKVR
CHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCEEEEHHHHH
DVIGIEIEPAVMVRILTQLGFKVEQQADSLICMPPSYRFDMSIKEDLIEEIARIYGYDNI
HHHCCCCCHHHHHHHHHHHCCEEHHCCCCEEECCCCCCCCCCHHHHHHHHHHHHCCCCCC
PSVLPHLQVSMDYDDTADLTHEMKLSLVDNGYMEAISFSFSDAKIEALLDDEALGEVLAL
HHHHCCEEEEECCCCCCCCHHHHEEEEECCCCCHHEEECCCCCEEEEEECHHHHHHHHHH
ANPISSDLAVMRRTLLSSLLPCVQYNLNRQQPRVRFFETGLSFVGQSISELVQTPSIALV
CCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEE
AVGDIWDEQAYQNRALDFYDLKHDIEQLLPAQMDSARIRYERSELAFLHPGQSAKLYIDD
EECCHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCHHEEEECCCEEEECCCCCCEEEECC
QYVGWLGQLHPNTAKQLDLPATWVAQLSLAPLLTLAREQHAITTPSKFPQVRRDIAILVD
HHHHHHHCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCEEEEEE
SDISLQTLESTIRKASGALLTDLWLFDVYQGEKVPAGQRSLAFALIWQDKMQTLSDDAVK
CCCCHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCEEEEEEEHHHHHHHHHHHHH
TVTDKVVQALTVEHSAQLRDS
HHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA