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The map label for this gene is hupR1 [H]

Identifier: 93007112

GI number: 93007112

Start: 2814783

End: 2815751

Strand: Direct

Name: hupR1 [H]

Synonym: Pcryo_2288

Alternate gene names: 93007112

Gene position: 2814783-2815751 (Clockwise)

Preceding gene: 93007111

Following gene: 93007113

Centisome position: 91.99

GC content: 42.41

Gene sequence:

>969_bases
ATGAATGAATTAGAAAATGACACCAGTAATAACATCAGTACCGCTGAGTTAGGGGTTGAAGCGAAACCGAAGCTGGCTTT
TATTGACGATGAACAACGTATTTTACGTTCGATGAAACTGTTGTTTCGTAAAACGCATGACGTTTTTATTACCACTGACC
CGACTGAATACATCGACTATATCAAAAACAACCATGTACACGTTGCAGTCAGTGACCAACGTATGCCTGAGCGGGTTGGC
GTTGATATCTTGCGCGAAGTAAAAGACGTATCACCTTCTACCATGCGTATATTATTAACCGGCTATGCTGATTTAAACGC
GATTATTGGCTCTATTAATGATGGTGAAATTTACCGTTATTTAACCAAGCCATGTAAATCTGAAGAACTGCTCGCTGTCG
TCGGACGCGCCACTGAAATCGCTTTGACTTATAACCCAGACGAAAACGCTGAGCAATTTGACAGCTCAGGCAACAAGCAA
ACGCTATTGGTCATTGATGAGACCAATGAGTTGATCGAGCAAATACGTAAGCAATTCTCTAATAACTATAAAGTGCTGCA
CGCCCAAGGTTTAGAGGAAGCTTACGATTATTTAGCGAACGAAGATATCGGTGTTTGTATTACCGACATCAATGTCAGTG
GCGAAAATATCGCGCCGATTATCTTTACCCTAAAGCAAGATGCGCCGCATCTGGTGGTCTTGGTACAGACCGAATTTCAA
GATGCTGGCTTGCTGATTGATTTGATTAATAAAGGTCAGGTTTATCGCTGCTTACCAAAACCAATGCGCGCAAGCCTCCT
TGAAATTAGTGTCAATCGTGCATTCCAGCACCATGCAAAACTAAAAGCCAGCCCTGAATTGGTCAAACGTTATGAAGTCG
AGCACGATCCTGAAAACGATGTGCGCATTGACTTGAGCAGTCGTGTACGTAGTCTGATCGGTAAATTGCGTAAGCGTTTT
TCTTTATAA

Upstream 100 bases:

>100_bases
AAGCCTAGCGGCATAATCGCTAGGTTCTGTTGTTATTTTAATTGCTATTTTAATGATTATATAAAACTATATCACTGTTA
TTCATCATAAGGATATCATC

Downstream 100 bases:

>100_bases
GCGCAAAAAAATAAGTAAGCGCAAGAAACGAGTGTCTCAATAAATAAAACCTCTATTTCAGCTTGAAGTAGAGGTTTTTG
CTTTGCGCTTTTTGACATGT

Product: response regulator receiver domain-containing protein

Products: NADP; 2 glutathione [C]

Alternate protein names: NA

Number of amino acids: Translated: 322; Mature: 322

Protein sequence:

>322_residues
MNELENDTSNNISTAELGVEAKPKLAFIDDEQRILRSMKLLFRKTHDVFITTDPTEYIDYIKNNHVHVAVSDQRMPERVG
VDILREVKDVSPSTMRILLTGYADLNAIIGSINDGEIYRYLTKPCKSEELLAVVGRATEIALTYNPDENAEQFDSSGNKQ
TLLVIDETNELIEQIRKQFSNNYKVLHAQGLEEAYDYLANEDIGVCITDINVSGENIAPIIFTLKQDAPHLVVLVQTEFQ
DAGLLIDLINKGQVYRCLPKPMRASLLEISVNRAFQHHAKLKASPELVKRYEVEHDPENDVRIDLSSRVRSLIGKLRKRF
SL

Sequences:

>Translated_322_residues
MNELENDTSNNISTAELGVEAKPKLAFIDDEQRILRSMKLLFRKTHDVFITTDPTEYIDYIKNNHVHVAVSDQRMPERVG
VDILREVKDVSPSTMRILLTGYADLNAIIGSINDGEIYRYLTKPCKSEELLAVVGRATEIALTYNPDENAEQFDSSGNKQ
TLLVIDETNELIEQIRKQFSNNYKVLHAQGLEEAYDYLANEDIGVCITDINVSGENIAPIIFTLKQDAPHLVVLVQTEFQ
DAGLLIDLINKGQVYRCLPKPMRASLLEISVNRAFQHHAKLKASPELVKRYEVEHDPENDVRIDLSSRVRSLIGKLRKRF
SL
>Mature_322_residues
MNELENDTSNNISTAELGVEAKPKLAFIDDEQRILRSMKLLFRKTHDVFITTDPTEYIDYIKNNHVHVAVSDQRMPERVG
VDILREVKDVSPSTMRILLTGYADLNAIIGSINDGEIYRYLTKPCKSEELLAVVGRATEIALTYNPDENAEQFDSSGNKQ
TLLVIDETNELIEQIRKQFSNNYKVLHAQGLEEAYDYLANEDIGVCITDINVSGENIAPIIFTLKQDAPHLVVLVQTEFQ
DAGLLIDLINKGQVYRCLPKPMRASLLEISVNRAFQHHAKLKASPELVKRYEVEHDPENDVRIDLSSRVRSLIGKLRKRF
SL

Specific function: Probable member of the two-component regulatory system involved in the regulation of the [NiFe] hydrogenase activity. HupR1 is probably phosphorylated by the sensory component and then acts in conjunction with sigma-54 as a transcriptional activator [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 36552; Mature: 36552

Theoretical pI: Translated: 5.05; Mature: 5.05

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNELENDTSNNISTAELGVEAKPKLAFIDDEQRILRSMKLLFRKTHDVFITTDPTEYIDY
CCCCCCCCCCCCEEHCCCCCCCCCEEEECCHHHHHHHHHHHHHCCCCEEEEECHHHHHHH
IKNNHVHVAVSDQRMPERVGVDILREVKDVSPSTMRILLTGYADLNAIIGSINDGEIYRY
HCCCCEEEEECCCCCCHHHHHHHHHHHHHCCCCEEEEEEECCHHHHHHHCCCCCCHHHHH
LTKPCKSEELLAVVGRATEIALTYNPDENAEQFDSSGNKQTLLVIDETNELIEQIRKQFS
HHCCCCCCHHHHHHCCCEEEEEEECCCCCHHHHHCCCCCEEEEEEECCHHHHHHHHHHHC
NNYKVLHAQGLEEAYDYLANEDIGVCITDINVSGENIAPIIFTLKQDAPHLVVLVQTEFQ
CCEEEEEECCHHHHHHHHCCCCCEEEEEEEECCCCCCEEEEEEEECCCCEEEEEEEECCC
DAGLLIDLINKGQVYRCLPKPMRASLLEISVNRAFQHHAKLKASPELVKRYEVEHDPEND
CCCEEEEECCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCC
VRIDLSSRVRSLIGKLRKRFSL
EEEEHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNELENDTSNNISTAELGVEAKPKLAFIDDEQRILRSMKLLFRKTHDVFITTDPTEYIDY
CCCCCCCCCCCCEEHCCCCCCCCCEEEECCHHHHHHHHHHHHHCCCCEEEEECHHHHHHH
IKNNHVHVAVSDQRMPERVGVDILREVKDVSPSTMRILLTGYADLNAIIGSINDGEIYRY
HCCCCEEEEECCCCCCHHHHHHHHHHHHHCCCCEEEEEEECCHHHHHHHCCCCCCHHHHH
LTKPCKSEELLAVVGRATEIALTYNPDENAEQFDSSGNKQTLLVIDETNELIEQIRKQFS
HHCCCCCCHHHHHHCCCEEEEEEECCCCCHHHHHCCCCCEEEEEEECCHHHHHHHHHHHC
NNYKVLHAQGLEEAYDYLANEDIGVCITDINVSGENIAPIIFTLKQDAPHLVVLVQTEFQ
CCEEEEEECCHHHHHHHHCCCCCEEEEEEEECCCCCCEEEEEEEECCCCEEEEEEEECCC
DAGLLIDLINKGQVYRCLPKPMRASLLEISVNRAFQHHAKLKASPELVKRYEVEHDPEND
CCCEEEEECCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCC
VRIDLSSRVRSLIGKLRKRFSL
EEEEHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH2; oxidized glutathione [C]

Specific reaction: NADPH2 + oxidized glutathione = NADP + 2 glutathione [C]

General reaction: Oxidoreductases; Acting on NADH or NADPH; With a disulfide as acceptor [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1885559; 8497190; 2007559 [H]