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The map label for this gene is zraS [H]

Identifier: 93007111

GI number: 93007111

Start: 2812386

End: 2814698

Strand: Direct

Name: zraS [H]

Synonym: Pcryo_2287

Alternate gene names: 93007111

Gene position: 2812386-2814698 (Clockwise)

Preceding gene: 93007108

Following gene: 93007112

Centisome position: 91.91

GC content: 42.97

Gene sequence:

>2313_bases
ATGAGTGCCTCTAAAAAAGTTGGCTTTTTTAATCAAGTCAGTACCCAAGTTACCACTATTTTGTTCATTGCTTTTGCGGT
CGTACTTGCGGTCGTTGTCTATGTCGTTGATAAAGAAGGCCATGATAAAATCCGTCTTGAGTCAGCAAAGCTGGTCGCTG
AACGTGGTAATACCGCGGTTAATAGTATCTCAGCCGACATCAATCGTGTCATGCGTAGTGCCTTGCTGTTACAGCAAAGC
GCGCAAACGCTGCCACCGCAAGAGAATATTTTGCAAGTCAGTACCGCCAATGCATTCGATAAACGCGACTATAACTTATT
GGGTAGTGGCGGCATTTGGCCAGAAAAAGGCGCTCTTGGTGCCAATACTGCAACCCATCCGTTTTTGATGGTACGCCAAA
ACGGTGATTATCAAGCGGTACTAAGCGATCCAAACCCAACCAATCCTTATTATCAAGAAGATTGGTTTAGCGTCTTAAAA
CTACTCAAACCTGACAGCTGTATCTGGAACCATGTTCGCGCCAGCCAAACCAAAGGCGAGCTTGCCATGAGCTGCGGTGT
CGCAATTGAGCGTGACAATCAGTTTTGGGGTGCCAGTACGGTTAACTTTACCCTAAACCAGTTGCAAGAGAACATCGTCA
AGTTGAGTGAGAGCTCTGGTTCAGGCTACATTTTATTATTAGATAACAGTGATAAGGTAATCGCCTCATCTAACCCTGAA
CGCTTAAGCTATATTGATGAAGCAACCGGTACACCACTTGATATCAAACAAGTTATCAACTCTGACCCTGCTTGGAATCC
GGTATTAAACTTCTTGGAAACTCAGCGTAACGAGATTATCGAGCAAGTTGCAGCTAGCGTGTCACCGCAAGTTCAGCAAG
TATTGAATACAATTGATAAAGCAGAAACAGGTGATTCCAAAGGTTATTATTTGGTCAACTATGCTGCTTACCTAAATAAC
GGTGAAAACAAGCAAAATGCTATGGATAGTCGCTTGCTACAAAGCTTTGAGCTGACCAATGATAACTTTTATAACGGCAG
TCAAGCCTATGTCTTTGAGATTCCTGAAACCTATTGGAAACTTATCGTTGTCCAACCTGATGCTGAGATTAACGCCATTG
CCGACAATTTAAGTGAAAACTTGATTAACTGGATTGCGTTAGCGCTCCTTATCACCGCTGGTATTATCTACTTTATGCTA
ACTTTCTTAGCATTCAAGCCATTGAAAGAAACCACTGATAAAATTTCTCTCGCAGAAAACATCATTAATAACAAGCAGTA
CAACAAGCTAAGCTCAGTTAAATTTAAGACAGGTCGCAACGAGATTGGACTGGTAAACGGCTCTATTAACGACCTATTAG
ATCGTATCCAATCTAACGAAGGTAAGCTTGCCAATATCAATCAGCAGCTAGAAATTAAAGTAGAAGAACGTACTGCTGAG
CTGCAAGATACGCTAAAAGAGCTGAAAAACTCACAGCTGCAATTGATTCGTTCAGAAAAAATGGCGACGTTAGGTCAAAT
GGTTGCTGGTGTTGCTCATGAAGTAAATACGCCATTATCTTATGTGCAAAATAACCTTGAAATTATCGGACAGCTAACTG
AGCAATACGAAGAGCTGATTGAACTGGTACAAGGGTTAAAAGCCGTTAAAACTGATGCCGCTGCTGATGGCAAAATTGAC
AAGCTATTGGCAGATATCGTCCGTGCGTCTGATGAAATCCAAGAAGATGATTTGTCCGCTGAGCTAAAAGAGCTGATTAA
AGACTCGCTATTTGGCGTCGAGCAAATCACCGAGATGGTGCTTAATCTTCGTAACTTTGCCCGTCTTGATGAGTCAAAAG
TGAAGACCATTGATGTGCGCGAATGTATCGAAGCCTCGCTGAAAATCGCGGGTAACTCTATCAGACATCAAGAGATTGTC
ACTGATTTTGCGCCGACACCTGAAATTAAATGCTCACCGTCACAGATTAACCAAGTCTTGGTGAACTTGCTTAATAACGC
CGCGCAAGCCATGGGCGAAAAATCTGATGGCAAAATCGAAGTGCGCACCCGTGCCGATGACAGCAATGTTTATGTTGATG
TCATTGATAACGGTAAAGGCATGAGCTCAGAAGTGCTCAGTCAGATATTTGAGCCGTTCTTTACCACTAAAGGCGCAGGC
GAAGGTACTGGTCTTGGTATGGCAATCAGTCAGCAGATTATGGAGCAGCATAATGGCGACATTAAAGCCGTATCGACTGT
AGGCGTTGGCACAACCTTTACCTTAACGCTACCCATTAATAATGATCTGACAGAAAAAAGCCTAGCGGCATAA

Upstream 100 bases:

>100_bases
CAAGCCATTTTGATGAAACTTAACCATATATTTAGTTGTAGCCAATCCATCCCATTGGCAATTCTTTTTGTGCTCCCTAC
TCCCAAGGACAAATCCCGTC

Downstream 100 bases:

>100_bases
TCGCTAGGTTCTGTTGTTATTTTAATTGCTATTTTAATGATTATATAAAACTATATCACTGTTATTCATCATAAGGATAT
CATCATGAATGAATTAGAAA

Product: histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 770; Mature: 769

Protein sequence:

>770_residues
MSASKKVGFFNQVSTQVTTILFIAFAVVLAVVVYVVDKEGHDKIRLESAKLVAERGNTAVNSISADINRVMRSALLLQQS
AQTLPPQENILQVSTANAFDKRDYNLLGSGGIWPEKGALGANTATHPFLMVRQNGDYQAVLSDPNPTNPYYQEDWFSVLK
LLKPDSCIWNHVRASQTKGELAMSCGVAIERDNQFWGASTVNFTLNQLQENIVKLSESSGSGYILLLDNSDKVIASSNPE
RLSYIDEATGTPLDIKQVINSDPAWNPVLNFLETQRNEIIEQVAASVSPQVQQVLNTIDKAETGDSKGYYLVNYAAYLNN
GENKQNAMDSRLLQSFELTNDNFYNGSQAYVFEIPETYWKLIVVQPDAEINAIADNLSENLINWIALALLITAGIIYFML
TFLAFKPLKETTDKISLAENIINNKQYNKLSSVKFKTGRNEIGLVNGSINDLLDRIQSNEGKLANINQQLEIKVEERTAE
LQDTLKELKNSQLQLIRSEKMATLGQMVAGVAHEVNTPLSYVQNNLEIIGQLTEQYEELIELVQGLKAVKTDAAADGKID
KLLADIVRASDEIQEDDLSAELKELIKDSLFGVEQITEMVLNLRNFARLDESKVKTIDVRECIEASLKIAGNSIRHQEIV
TDFAPTPEIKCSPSQINQVLVNLLNNAAQAMGEKSDGKIEVRTRADDSNVYVDVIDNGKGMSSEVLSQIFEPFFTTKGAG
EGTGLGMAISQQIMEQHNGDIKAVSTVGVGTTFTLTLPINNDLTEKSLAA

Sequences:

>Translated_770_residues
MSASKKVGFFNQVSTQVTTILFIAFAVVLAVVVYVVDKEGHDKIRLESAKLVAERGNTAVNSISADINRVMRSALLLQQS
AQTLPPQENILQVSTANAFDKRDYNLLGSGGIWPEKGALGANTATHPFLMVRQNGDYQAVLSDPNPTNPYYQEDWFSVLK
LLKPDSCIWNHVRASQTKGELAMSCGVAIERDNQFWGASTVNFTLNQLQENIVKLSESSGSGYILLLDNSDKVIASSNPE
RLSYIDEATGTPLDIKQVINSDPAWNPVLNFLETQRNEIIEQVAASVSPQVQQVLNTIDKAETGDSKGYYLVNYAAYLNN
GENKQNAMDSRLLQSFELTNDNFYNGSQAYVFEIPETYWKLIVVQPDAEINAIADNLSENLINWIALALLITAGIIYFML
TFLAFKPLKETTDKISLAENIINNKQYNKLSSVKFKTGRNEIGLVNGSINDLLDRIQSNEGKLANINQQLEIKVEERTAE
LQDTLKELKNSQLQLIRSEKMATLGQMVAGVAHEVNTPLSYVQNNLEIIGQLTEQYEELIELVQGLKAVKTDAAADGKID
KLLADIVRASDEIQEDDLSAELKELIKDSLFGVEQITEMVLNLRNFARLDESKVKTIDVRECIEASLKIAGNSIRHQEIV
TDFAPTPEIKCSPSQINQVLVNLLNNAAQAMGEKSDGKIEVRTRADDSNVYVDVIDNGKGMSSEVLSQIFEPFFTTKGAG
EGTGLGMAISQQIMEQHNGDIKAVSTVGVGTTFTLTLPINNDLTEKSLAA
>Mature_769_residues
SASKKVGFFNQVSTQVTTILFIAFAVVLAVVVYVVDKEGHDKIRLESAKLVAERGNTAVNSISADINRVMRSALLLQQSA
QTLPPQENILQVSTANAFDKRDYNLLGSGGIWPEKGALGANTATHPFLMVRQNGDYQAVLSDPNPTNPYYQEDWFSVLKL
LKPDSCIWNHVRASQTKGELAMSCGVAIERDNQFWGASTVNFTLNQLQENIVKLSESSGSGYILLLDNSDKVIASSNPER
LSYIDEATGTPLDIKQVINSDPAWNPVLNFLETQRNEIIEQVAASVSPQVQQVLNTIDKAETGDSKGYYLVNYAAYLNNG
ENKQNAMDSRLLQSFELTNDNFYNGSQAYVFEIPETYWKLIVVQPDAEINAIADNLSENLINWIALALLITAGIIYFMLT
FLAFKPLKETTDKISLAENIINNKQYNKLSSVKFKTGRNEIGLVNGSINDLLDRIQSNEGKLANINQQLEIKVEERTAEL
QDTLKELKNSQLQLIRSEKMATLGQMVAGVAHEVNTPLSYVQNNLEIIGQLTEQYEELIELVQGLKAVKTDAAADGKIDK
LLADIVRASDEIQEDDLSAELKELIKDSLFGVEQITEMVLNLRNFARLDESKVKTIDVRECIEASLKIAGNSIRHQEIVT
DFAPTPEIKCSPSQINQVLVNLLNNAAQAMGEKSDGKIEVRTRADDSNVYVDVIDNGKGMSSEVLSQIFEPFFTTKGAGE
GTGLGMAISQQIMEQHNGDIKAVSTVGVGTTFTLTLPINNDLTEKSLAA

Specific function: Member of the two-component regulatory system zraS/zraR. May function as a membrane-associated protein kinase that phosphorylates zraR in response to high concentrations of zinc or lead in the medium [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1790436, Length=281, Percent_Identity=32.0284697508897, Blast_Score=131, Evalue=2e-31,
Organism=Escherichia coli, GI1788549, Length=285, Percent_Identity=32.6315789473684, Blast_Score=130, Evalue=3e-31,
Organism=Escherichia coli, GI87081816, Length=324, Percent_Identity=27.7777777777778, Blast_Score=100, Evalue=4e-22,
Organism=Escherichia coli, GI145693157, Length=285, Percent_Identity=25.6140350877193, Blast_Score=80, Evalue=6e-16,
Organism=Escherichia coli, GI1790300, Length=134, Percent_Identity=35.8208955223881, Blast_Score=77, Evalue=4e-15,
Organism=Escherichia coli, GI1790346, Length=274, Percent_Identity=24.8175182481752, Blast_Score=72, Evalue=1e-13,
Organism=Escherichia coli, GI1788713, Length=266, Percent_Identity=26.3157894736842, Blast_Score=70, Evalue=4e-13,
Organism=Escherichia coli, GI1788393, Length=247, Percent_Identity=24.2914979757085, Blast_Score=68, Evalue=3e-12,
Organism=Escherichia coli, GI48994928, Length=120, Percent_Identity=31.6666666666667, Blast_Score=67, Evalue=4e-12,
Organism=Escherichia coli, GI87082128, Length=110, Percent_Identity=34.5454545454545, Blast_Score=67, Evalue=6e-12,
Organism=Escherichia coli, GI1789808, Length=134, Percent_Identity=31.3432835820896, Blast_Score=65, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 84906; Mature: 84775

Theoretical pI: Translated: 4.47; Mature: 4.47

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSASKKVGFFNQVSTQVTTILFIAFAVVLAVVVYVVDKEGHDKIRLESAKLVAERGNTAV
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEHHHHHHHHCCCHHH
NSISADINRVMRSALLLQQSAQTLPPQENILQVSTANAFDKRDYNLLGSGGIWPEKGALG
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHEEEEECCCCCCCCCCCEECCCCCCCCCCCCC
ANTATHPFLMVRQNGDYQAVLSDPNPTNPYYQEDWFSVLKLLKPDSCIWNHVRASQTKGE
CCCCCCCEEEEEECCCEEEEECCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHCCCCCC
LAMSCGVAIERDNQFWGASTVNFTLNQLQENIVKLSESSGSGYILLLDNSDKVIASSNPE
HHHHCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCEEECCCCH
RLSYIDEATGTPLDIKQVINSDPAWNPVLNFLETQRNEIIEQVAASVSPQVQQVLNTIDK
HHHHHHHCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
AETGDSKGYYLVNYAAYLNNGENKQNAMDSRLLQSFELTNDNFYNGSQAYVFEIPETYWK
CCCCCCCCEEEEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCEE
LIVVQPDAEINAIADNLSENLINWIALALLITAGIIYFMLTFLAFKPLKETTDKISLAEN
EEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IINNKQYNKLSSVKFKTGRNEIGLVNGSINDLLDRIQSNEGKLANINQQLEIKVEERTAE
HHCCHHHCHHHCEEEECCCCCEEEECCCHHHHHHHHHCCCCCEECCCCEEEEEEHHHHHH
LQDTLKELKNSQLQLIRSEKMATLGQMVAGVAHEVNTPLSYVQNNLEIIGQLTEQYEELI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
ELVQGLKAVKTDAAADGKIDKLLADIVRASDEIQEDDLSAELKELIKDSLFGVEQITEMV
HHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
LNLRNFARLDESKVKTIDVRECIEASLKIAGNSIRHQEIVTDFAPTPEIKCSPSQINQVL
HHHHHHHHCCHHHCCEECHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHH
VNLLNNAAQAMGEKSDGKIEVRTRADDSNVYVDVIDNGKGMSSEVLSQIFEPFFTTKGAG
HHHHHHHHHHCCCCCCCEEEEEEECCCCCEEEEEECCCCCCHHHHHHHHHHHHHCCCCCC
EGTGLGMAISQQIMEQHNGDIKAVSTVGVGTTFTLTLPINNDLTEKSLAA
CCCCCHHHHHHHHHHHCCCCEEEEEEECCCEEEEEEEECCCCCCHHHHCC
>Mature Secondary Structure 
SASKKVGFFNQVSTQVTTILFIAFAVVLAVVVYVVDKEGHDKIRLESAKLVAERGNTAV
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEHHHHHHHHCCCHHH
NSISADINRVMRSALLLQQSAQTLPPQENILQVSTANAFDKRDYNLLGSGGIWPEKGALG
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHEEEEECCCCCCCCCCCEECCCCCCCCCCCCC
ANTATHPFLMVRQNGDYQAVLSDPNPTNPYYQEDWFSVLKLLKPDSCIWNHVRASQTKGE
CCCCCCCEEEEEECCCEEEEECCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHCCCCCC
LAMSCGVAIERDNQFWGASTVNFTLNQLQENIVKLSESSGSGYILLLDNSDKVIASSNPE
HHHHCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCEEECCCCH
RLSYIDEATGTPLDIKQVINSDPAWNPVLNFLETQRNEIIEQVAASVSPQVQQVLNTIDK
HHHHHHHCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
AETGDSKGYYLVNYAAYLNNGENKQNAMDSRLLQSFELTNDNFYNGSQAYVFEIPETYWK
CCCCCCCCEEEEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCEE
LIVVQPDAEINAIADNLSENLINWIALALLITAGIIYFMLTFLAFKPLKETTDKISLAEN
EEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IINNKQYNKLSSVKFKTGRNEIGLVNGSINDLLDRIQSNEGKLANINQQLEIKVEERTAE
HHCCHHHCHHHCEEEECCCCCEEEECCCHHHHHHHHHCCCCCEECCCCEEEEEEHHHHHH
LQDTLKELKNSQLQLIRSEKMATLGQMVAGVAHEVNTPLSYVQNNLEIIGQLTEQYEELI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
ELVQGLKAVKTDAAADGKIDKLLADIVRASDEIQEDDLSAELKELIKDSLFGVEQITEMV
HHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
LNLRNFARLDESKVKTIDVRECIEASLKIAGNSIRHQEIVTDFAPTPEIKCSPSQINQVL
HHHHHHHHCCHHHCCEECHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHH
VNLLNNAAQAMGEKSDGKIEVRTRADDSNVYVDVIDNGKGMSSEVLSQIFEPFFTTKGAG
HHHHHHHHHHCCCCCCCEEEEEEECCCCCEEEEEECCCCCCHHHHHHHHHHHHHCCCCCC
EGTGLGMAISQQIMEQHNGDIKAVSTVGVGTTFTLTLPINNDLTEKSLAA
CCCCCHHHHHHHHHHHCCCCEEEEEEECCCEEEEEEEECCCCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8265357; 9278503; 2666400; 11243806 [H]