Click here to switch to the map view.

The map label for this gene is alsT [H]

Identifier: 93007076

GI number: 93007076

Start: 2762930

End: 2764426

Strand: Reverse

Name: alsT [H]

Synonym: Pcryo_2252

Alternate gene names: 93007076

Gene position: 2764426-2762930 (Counterclockwise)

Preceding gene: 93007080

Following gene: 93007074

Centisome position: 90.34

GC content: 43.62

Gene sequence:

>1497_bases
ATGAACGAAGGCGTAGCAGGTTGGTTTAATAGCATCGTAAACTATGGTGTGAGTATCATTTGGGGTAATAATGATTGGTT
GATTGCCAGCAATCCATGGTATGGACTGCTGATGTTCGTCTTAATTGGTGCAGGTCTATATTTTACCGTGGCGACTCGCT
TTATCCAGTTTCGTCATTTTGGTCATATGTGGCAGTTATTACGAGTATCAAATCAAGGGCGTAAAGATGGTGGTATCAGC
TCATTTGAAGCACTGATGACATCACTTGCTGCGCGAGTAGGGACGGGGAATCTAGCAGGGGTGGCAATTGCCATCTATTT
GGGTGGACCGGGTGCTGTATTTTGGATGTGGATGACAGCGATTGTGGGTATGTCAACCAGCTTTATCGAGTCAACCCTTG
CGCAAGCATACAAAGTGCCGCATAACGATAATGTTTATCGCGGTGGACCGGCTTACTATATCGAAAAGGGTTTGGGTAAA
CGCTGGCTTGCCGTATTTTTCTCGCTATGTCTGCTGGTCGCTTTTGGCTTGGCGTTCAATGGTGTGCAATCCAATACCAT
TGCACAAGCGACTAATGAGGCGTTTGGTATTCCAACTTGGATGACCGGCTTGATATTAGTAGTGCTAGTCGCACCAGTAG
TATTTGGTGGATTACGCTCTGTTGCGCGTATTGCTGGCAAAGTTGTACCAGTGATGGCGATTCTTTATATTCTGTTGGCA
CTCTATGTCATCTTGACCAATTTTAGCGAATTTCCAGCGGCGATTGCTTTGATTGTAAAGTCAGCATTTGGCTTTGAACA
AGCAGCAGGTGGTGTGATTGGTTATGGTATTGCGCAAGCGATGATTAATGGTATTAAGCGTGGTCTATTTTCAAACGAAG
CAGGTATGGGTTCAGCACCTAATGCCGCAGCGACTGCAAAGAGCCATCCTGATCATCCGGCAGTGCAAGGTTTTATGCAA
ATGTTGGGTGTGTTTTTGGACACATTAGTTATTTGTACAGCGACAGCTTCAATTATTATTCTCTCTGGCGTGGTTGATCC
TAGTGTCGAGCAAGAAGGTATTCAATTAACACAGCTAGCATTGTCGCAATATGTAGGTGATATGGGTGTAATTTTCGTCG
CGATTGCCATCTTCTTTTTCTCATTCACCTCTATCATTGCAAACTATAGCTATGGTGAATCGAATCTTGAATTTATCTCT
GGCGAAAAAAATGCCAAAGTGATGATTATGATATTCCGCTTTATGGTCTTGGGCATGGTATTTATCGGTGCTGTCGCCAG
CCTGCCAGCTATTTGGAACTTTGCCGATTTATCCATGGGTCTGATGGCATTGGTCAACTTAGTGGCTATTATCATCTTGT
CGCCAGTAGCACTTAGAATATTACGTGACTATGAGCGTCAGATTAAAGAGGGCAAAACTGCTGATCAAATCAAGTTTAAT
CCTGATGATTTTGTGAAATTAAAAGATGAAGCCAATCGTGATGCGTGGACAGATTAA

Upstream 100 bases:

>100_bases
TCAATAAGCAAAGAGAGAACGAAACATCATAAAAAGCCATTAGTTACATCAATTTATAACATATGCTAAAAGTATAGAAT
GTTAGTGAAGGGGATATAGT

Downstream 100 bases:

>100_bases
GCTGAAATCCTAGATATACAGCGATTAAATATACCTATAACAAAAAATCGCCCTTTTGAGGGCGATTTTTTTTGCAGCTT
ATTTTAAATAATAACCATCT

Product: amino acid carrier protein

Products: Na (I) [Cytoplasm]; L-alanine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 498; Mature: 498

Protein sequence:

>498_residues
MNEGVAGWFNSIVNYGVSIIWGNNDWLIASNPWYGLLMFVLIGAGLYFTVATRFIQFRHFGHMWQLLRVSNQGRKDGGIS
SFEALMTSLAARVGTGNLAGVAIAIYLGGPGAVFWMWMTAIVGMSTSFIESTLAQAYKVPHNDNVYRGGPAYYIEKGLGK
RWLAVFFSLCLLVAFGLAFNGVQSNTIAQATNEAFGIPTWMTGLILVVLVAPVVFGGLRSVARIAGKVVPVMAILYILLA
LYVILTNFSEFPAAIALIVKSAFGFEQAAGGVIGYGIAQAMINGIKRGLFSNEAGMGSAPNAAATAKSHPDHPAVQGFMQ
MLGVFLDTLVICTATASIIILSGVVDPSVEQEGIQLTQLALSQYVGDMGVIFVAIAIFFFSFTSIIANYSYGESNLEFIS
GEKNAKVMIMIFRFMVLGMVFIGAVASLPAIWNFADLSMGLMALVNLVAIIILSPVALRILRDYERQIKEGKTADQIKFN
PDDFVKLKDEANRDAWTD

Sequences:

>Translated_498_residues
MNEGVAGWFNSIVNYGVSIIWGNNDWLIASNPWYGLLMFVLIGAGLYFTVATRFIQFRHFGHMWQLLRVSNQGRKDGGIS
SFEALMTSLAARVGTGNLAGVAIAIYLGGPGAVFWMWMTAIVGMSTSFIESTLAQAYKVPHNDNVYRGGPAYYIEKGLGK
RWLAVFFSLCLLVAFGLAFNGVQSNTIAQATNEAFGIPTWMTGLILVVLVAPVVFGGLRSVARIAGKVVPVMAILYILLA
LYVILTNFSEFPAAIALIVKSAFGFEQAAGGVIGYGIAQAMINGIKRGLFSNEAGMGSAPNAAATAKSHPDHPAVQGFMQ
MLGVFLDTLVICTATASIIILSGVVDPSVEQEGIQLTQLALSQYVGDMGVIFVAIAIFFFSFTSIIANYSYGESNLEFIS
GEKNAKVMIMIFRFMVLGMVFIGAVASLPAIWNFADLSMGLMALVNLVAIIILSPVALRILRDYERQIKEGKTADQIKFN
PDDFVKLKDEANRDAWTD
>Mature_498_residues
MNEGVAGWFNSIVNYGVSIIWGNNDWLIASNPWYGLLMFVLIGAGLYFTVATRFIQFRHFGHMWQLLRVSNQGRKDGGIS
SFEALMTSLAARVGTGNLAGVAIAIYLGGPGAVFWMWMTAIVGMSTSFIESTLAQAYKVPHNDNVYRGGPAYYIEKGLGK
RWLAVFFSLCLLVAFGLAFNGVQSNTIAQATNEAFGIPTWMTGLILVVLVAPVVFGGLRSVARIAGKVVPVMAILYILLA
LYVILTNFSEFPAAIALIVKSAFGFEQAAGGVIGYGIAQAMINGIKRGLFSNEAGMGSAPNAAATAKSHPDHPAVQGFMQ
MLGVFLDTLVICTATASIIILSGVVDPSVEQEGIQLTQLALSQYVGDMGVIFVAIAIFFFSFTSIIANYSYGESNLEFIS
GEKNAKVMIMIFRFMVLGMVFIGAVASLPAIWNFADLSMGLMALVNLVAIIILSPVALRILRDYERQIKEGKTADQIKFN
PDDFVKLKDEANRDAWTD

Specific function: Unknown

COG id: COG1115

COG function: function code E; Na+/alanine symporter

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium:alanine (SAF) symporter family [H]

Homologues:

Organism=Escherichia coli, GI1786188, Length=471, Percent_Identity=46.2845010615711, Blast_Score=381, Evalue=1e-107,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001463 [H]

Pfam domain/function: PF01235 Na_Ala_symp [H]

EC number: NA

Molecular weight: Translated: 53833; Mature: 53833

Theoretical pI: Translated: 7.06; Mature: 7.06

Prosite motif: PS00873 NA_ALANINE_SYMP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNEGVAGWFNSIVNYGVSIIWGNNDWLIASNPWYGLLMFVLIGAGLYFTVATRFIQFRHF
CCCCHHHHHHHHHHCCEEEEECCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GHMWQLLRVSNQGRKDGGISSFEALMTSLAARVGTGNLAGVAIAIYLGGPGAVFWMWMTA
HHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCHHHHHHHHHH
IVGMSTSFIESTLAQAYKVPHNDNVYRGGPAYYIEKGLGKRWLAVFFSLCLLVAFGLAFN
HHCCCHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHC
GVQSNTIAQATNEAFGIPTWMTGLILVVLVAPVVFGGLRSVARIAGKVVPVMAILYILLA
CCCCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LYVILTNFSEFPAAIALIVKSAFGFEQAAGGVIGYGIAQAMINGIKRGLFSNEAGMGSAP
HHHHHHCCHHHHHHHHHHHHHHCCHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
NAAATAKSHPDHPAVQGFMQMLGVFLDTLVICTATASIIILSGVVDPSVEQEGIQLTQLA
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHH
LSQYVGDMGVIFVAIAIFFFSFTSIIANYSYGESNLEFISGEKNAKVMIMIFRFMVLGMV
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEECCCCCCHHHHHHHHHHHHHHH
FIGAVASLPAIWNFADLSMGLMALVNLVAIIILSPVALRILRDYERQIKEGKTADQIKFN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEECC
PDDFVKLKDEANRDAWTD
CHHHEEECCCCCCCCCCC
>Mature Secondary Structure
MNEGVAGWFNSIVNYGVSIIWGNNDWLIASNPWYGLLMFVLIGAGLYFTVATRFIQFRHF
CCCCHHHHHHHHHHCCEEEEECCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GHMWQLLRVSNQGRKDGGISSFEALMTSLAARVGTGNLAGVAIAIYLGGPGAVFWMWMTA
HHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCHHHHHHHHHH
IVGMSTSFIESTLAQAYKVPHNDNVYRGGPAYYIEKGLGKRWLAVFFSLCLLVAFGLAFN
HHCCCHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHC
GVQSNTIAQATNEAFGIPTWMTGLILVVLVAPVVFGGLRSVARIAGKVVPVMAILYILLA
CCCCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LYVILTNFSEFPAAIALIVKSAFGFEQAAGGVIGYGIAQAMINGIKRGLFSNEAGMGSAP
HHHHHHCCHHHHHHHHHHHHHHCCHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
NAAATAKSHPDHPAVQGFMQMLGVFLDTLVICTATASIIILSGVVDPSVEQEGIQLTQLA
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHH
LSQYVGDMGVIFVAIAIFFFSFTSIIANYSYGESNLEFISGEKNAKVMIMIFRFMVLGMV
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEECCCCCCHHHHHHHHHHHHHHH
FIGAVASLPAIWNFADLSMGLMALVNLVAIIILSPVALRILRDYERQIKEGKTADQIKFN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEECC
PDDFVKLKDEANRDAWTD
CHHHEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Na (I) [Periplasm]; L-alanine [Periplasm] [C]

Specific reaction: Na (I) [Periplasm] + L-alanine [Periplasm] = Na (I) [Cytoplasm] + L-alanine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8969507; 9384377; 7710280 [H]