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The map label for this gene is ahpF [H]

Identifier: 93006898

GI number: 93006898

Start: 2540825

End: 2542402

Strand: Reverse

Name: ahpF [H]

Synonym: Pcryo_2074

Alternate gene names: 93006898

Gene position: 2542402-2540825 (Counterclockwise)

Preceding gene: 93006902

Following gene: 93006891

Centisome position: 83.09

GC content: 43.22

Gene sequence:

>1578_bases
ATGATAGACCAAAGCTTATTAGATGCCGTAAAGAGCTATAGCGTAAATATGACCCGCCCAATCAGCTTTGTCTTAGGTAG
TGGCGAGCATAGCAAGCGTGCTGAGTTGATTGATTTTTTGAGCAAAATCGCTGGTACGACAGATAAGATTAATTTTGACG
CACAAGCTAACGATGACAGTTTGCCAAGCCCAATCAGTTTTAAAGTAATCAGTCACATTGATGGTGCTTTGACGGATACA
GGTATTGTCTTTAGTGGTATTCCTGGTGGTCATGAATTTACTTCATTGATATTGGCGATTTTGCAAGCGGGTGGTCATAC
GTTAAAACTCGATGAAGGCATTCAAAAGCTGATTAAACGCTTTAATAAGCCACTACAGTTTCAAACTTACGTGTCGCTTT
CTTGCCACAGCTGCCCAGAAGTTGTGCAAGCGCTAAACCAATTCGCGCTGCTAAACGATGACATCAGTAATGAGATGATC
GATGGGGCACTATTCCAAGAGCAAGTAGAAGCGAATAATATCCAAGGCGTACCAGCAGTATTCTTAAACGGCAAGCCGTT
TGCTAATGGTCTGATTGACACGGCTAAATTGATTGAGAAGTTACAAGAGCAATTTCCTGATTTGTTAGCAGAAGCTGAAG
ATGATGCTGAACAGTTAGAGCAACAAGATGTGACGATTATTGGTGCGGGTCCAGCTGGGGTTGCTGCTGCGATTTATACC
GCGCGTAAAGGCTTAAAAGTTACCATGGTGGCTGACCGTATCGGTGGGCAAGTAAAAGATACGCAAGATATTGAAAACTT
AATCTCAGTACCTTTAACCACAGGTGGCGAGCTCTCGGTGAACTTTGAAAAGCATTTGCGCGAATATAATATTACGCTTA
AAGAGCACGTCAGCGTCAAAGAGATTAGTGAGACAGCTGATGAAAATTATCGCATTCATCTGAATACCGGTGAGACCTTT
GAGACGCGTAGTATTATCTTGGCAACTGGTGCTCAATGGCGTAAGCTTGGGGTCGCTGGCGAAGAAGAAAACATCGGTAA
AGGCGTGGCTTTCTGTGCGCACTGTGATGGTCCATTCTTCAAAGGTAAAGATATTGCCGTCGTCGGTGGTGGTAACTCTG
GTATCGAAGCGGCATTAGACCTTGCTGGTATCGTGAAGCATGTGACCGTGCTTGAGTTTGCTGATGATTTGAAAGCGGAT
CAGGTATTGATTAATAAAGCCAAAGAAAAGGCAAATATCGATATCATTACCAGTGCAGCGACCCAAGAAATTAAAGCGAC
TGAGGGTAAAGTATCGTCTATTATCTATCAAGATCGCAATAGCAGCGAGAATAAAGAGCTTGATGTTGCAGGGGTGTTTG
TACAGATTGGCTTGATACCAAATACTGGGTTCGTTAAAGGCTTCGTCGATTTAAACCGCTTTGGCGAGATTGAAATTGAT
GAGCGCTGCCGTACAGATCGCAAAGGTATCTTTGCTTGTGGTGATGTTACGACTGTACCCTTTAAGCAGATTAATATTGC
GATGGGTGAAGGTTCAAAAGCGGCATTATCAGCGTTTGAATATTTGGTTATGCAGTAA

Upstream 100 bases:

>100_bases
ATGCCGCATATAAATAGCAGTATTGGCATATGACTCTTACTCATTTAAACGCGCAGCACTGATGCTGACGTTACATATTC
GATAATAACAAGGAACAATT

Downstream 100 bases:

>100_bases
GCCAGTCTTTAATATCTTCTAAAATAAAAAGCTCCGATATCCTAATAGGTATCGGAGCTTTGTTACTTATAGACGTTAAG
TAAATGCTATTTAGCCAATA

Product: FAD-dependent pyridine nucleotide-disulfide oxidoreductase

Products: an alcohol; H2O; NAD [C]

Alternate protein names: Alkyl hydroperoxide reductase F52A protein [H]

Number of amino acids: Translated: 525; Mature: 525

Protein sequence:

>525_residues
MIDQSLLDAVKSYSVNMTRPISFVLGSGEHSKRAELIDFLSKIAGTTDKINFDAQANDDSLPSPISFKVISHIDGALTDT
GIVFSGIPGGHEFTSLILAILQAGGHTLKLDEGIQKLIKRFNKPLQFQTYVSLSCHSCPEVVQALNQFALLNDDISNEMI
DGALFQEQVEANNIQGVPAVFLNGKPFANGLIDTAKLIEKLQEQFPDLLAEAEDDAEQLEQQDVTIIGAGPAGVAAAIYT
ARKGLKVTMVADRIGGQVKDTQDIENLISVPLTTGGELSVNFEKHLREYNITLKEHVSVKEISETADENYRIHLNTGETF
ETRSIILATGAQWRKLGVAGEEENIGKGVAFCAHCDGPFFKGKDIAVVGGGNSGIEAALDLAGIVKHVTVLEFADDLKAD
QVLINKAKEKANIDIITSAATQEIKATEGKVSSIIYQDRNSSENKELDVAGVFVQIGLIPNTGFVKGFVDLNRFGEIEID
ERCRTDRKGIFACGDVTTVPFKQINIAMGEGSKAALSAFEYLVMQ

Sequences:

>Translated_525_residues
MIDQSLLDAVKSYSVNMTRPISFVLGSGEHSKRAELIDFLSKIAGTTDKINFDAQANDDSLPSPISFKVISHIDGALTDT
GIVFSGIPGGHEFTSLILAILQAGGHTLKLDEGIQKLIKRFNKPLQFQTYVSLSCHSCPEVVQALNQFALLNDDISNEMI
DGALFQEQVEANNIQGVPAVFLNGKPFANGLIDTAKLIEKLQEQFPDLLAEAEDDAEQLEQQDVTIIGAGPAGVAAAIYT
ARKGLKVTMVADRIGGQVKDTQDIENLISVPLTTGGELSVNFEKHLREYNITLKEHVSVKEISETADENYRIHLNTGETF
ETRSIILATGAQWRKLGVAGEEENIGKGVAFCAHCDGPFFKGKDIAVVGGGNSGIEAALDLAGIVKHVTVLEFADDLKAD
QVLINKAKEKANIDIITSAATQEIKATEGKVSSIIYQDRNSSENKELDVAGVFVQIGLIPNTGFVKGFVDLNRFGEIEID
ERCRTDRKGIFACGDVTTVPFKQINIAMGEGSKAALSAFEYLVMQ
>Mature_525_residues
MIDQSLLDAVKSYSVNMTRPISFVLGSGEHSKRAELIDFLSKIAGTTDKINFDAQANDDSLPSPISFKVISHIDGALTDT
GIVFSGIPGGHEFTSLILAILQAGGHTLKLDEGIQKLIKRFNKPLQFQTYVSLSCHSCPEVVQALNQFALLNDDISNEMI
DGALFQEQVEANNIQGVPAVFLNGKPFANGLIDTAKLIEKLQEQFPDLLAEAEDDAEQLEQQDVTIIGAGPAGVAAAIYT
ARKGLKVTMVADRIGGQVKDTQDIENLISVPLTTGGELSVNFEKHLREYNITLKEHVSVKEISETADENYRIHLNTGETF
ETRSIILATGAQWRKLGVAGEEENIGKGVAFCAHCDGPFFKGKDIAVVGGGNSGIEAALDLAGIVKHVTVLEFADDLKAD
QVLINKAKEKANIDIITSAATQEIKATEGKVSSIIYQDRNSSENKELDVAGVFVQIGLIPNTGFVKGFVDLNRFGEIEID
ERCRTDRKGIFACGDVTTVPFKQINIAMGEGSKAALSAFEYLVMQ

Specific function: Serves to protect the cell against DNA damage by alkyl hydroperoxides. It can use either NADH or NADPH as electron donor for direct reduction of redox dyes or of alkyl hydroperoxides when combined with the AhpC protein [H]

COG id: COG3634

COG function: function code O; Alkyl hydroperoxide reductase, large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Escherichia coli, GI87081763, Length=527, Percent_Identity=45.73055028463, Blast_Score=451, Evalue=1e-128,
Organism=Escherichia coli, GI1787114, Length=315, Percent_Identity=33.015873015873, Blast_Score=157, Evalue=2e-39,
Organism=Caenorhabditis elegans, GI17559934, Length=306, Percent_Identity=27.4509803921569, Blast_Score=70, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6321898, Length=321, Percent_Identity=34.5794392523364, Blast_Score=143, Evalue=6e-35,
Organism=Saccharomyces cerevisiae, GI6320560, Length=314, Percent_Identity=33.7579617834395, Blast_Score=142, Evalue=2e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012081
- InterPro:   IPR013027
- InterPro:   IPR002109
- InterPro:   IPR008255
- InterPro:   IPR001327
- InterPro:   IPR000103
- InterPro:   IPR012336
- InterPro:   IPR012335 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: 1.6.4.- [C]

Molecular weight: Translated: 56747; Mature: 56747

Theoretical pI: Translated: 4.57; Mature: 4.57

Prosite motif: PS00573 PYRIDINE_REDOX_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIDQSLLDAVKSYSVNMTRPISFVLGSGEHSKRAELIDFLSKIAGTTDKINFDAQANDDS
CCCHHHHHHHHHCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHCCCCCCEEECCCCCCCC
LPSPISFKVISHIDGALTDTGIVFSGIPGGHEFTSLILAILQAGGHTLKLDEGIQKLIKR
CCCCCHHHHHHHCCCCCCCCCEEEECCCCCHHHHHHHHHHHHCCCCEEEHHHHHHHHHHH
FNKPLQFQTYVSLSCHSCPEVVQALNQFALLNDDISNEMIDGALFQEQVEANNIQGVPAV
CCCCCEEEEEEEECCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCEE
FLNGKPFANGLIDTAKLIEKLQEQFPDLLAEAEDDAEQLEQQDVTIIGAGPAGVAAAIYT
EECCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCEEEEECCCHHHHHHHHH
ARKGLKVTMVADRIGGQVKDTQDIENLISVPLTTGGELSVNFEKHLREYNITLKEHVSVK
HCCCCEEEEEEHHHCCCCCCHHHHHHHHHCCCCCCCEEEEEHHHHHHHHCCCHHHCCCHH
EISETADENYRIHLNTGETFETRSIILATGAQWRKLGVAGEEENIGKGVAFCAHCDGPFF
HHHHHCCCCEEEEECCCCCEECCEEEEECCCCHHEECCCCCCCCCCCCEEEEEECCCCCC
KGKDIAVVGGGNSGIEAALDLAGIVKHVTVLEFADDLKADQVLINKAKEKANIDIITSAA
CCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCEEEEECCH
TQEIKATEGKVSSIIYQDRNSSENKELDVAGVFVQIGLIPNTGFVKGFVDLNRFGEIEID
HHHHHHCCCHHHEEEEECCCCCCCCCEEEEEEEEEEEEECCCCCEEEEHHHCCCCCCEEH
ERCRTDRKGIFACGDVTTVPFKQINIAMGEGSKAALSAFEYLVMQ
HHHCCCCCCEEEECCCCCCCCEEEEEEECCCCHHHHHHHHHHHCC
>Mature Secondary Structure
MIDQSLLDAVKSYSVNMTRPISFVLGSGEHSKRAELIDFLSKIAGTTDKINFDAQANDDS
CCCHHHHHHHHHCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHCCCCCCEEECCCCCCCC
LPSPISFKVISHIDGALTDTGIVFSGIPGGHEFTSLILAILQAGGHTLKLDEGIQKLIKR
CCCCCHHHHHHHCCCCCCCCCEEEECCCCCHHHHHHHHHHHHCCCCEEEHHHHHHHHHHH
FNKPLQFQTYVSLSCHSCPEVVQALNQFALLNDDISNEMIDGALFQEQVEANNIQGVPAV
CCCCCEEEEEEEECCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCEE
FLNGKPFANGLIDTAKLIEKLQEQFPDLLAEAEDDAEQLEQQDVTIIGAGPAGVAAAIYT
EECCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCEEEEECCCHHHHHHHHH
ARKGLKVTMVADRIGGQVKDTQDIENLISVPLTTGGELSVNFEKHLREYNITLKEHVSVK
HCCCCEEEEEEHHHCCCCCCHHHHHHHHHCCCCCCCEEEEEHHHHHHHHCCCHHHCCCHH
EISETADENYRIHLNTGETFETRSIILATGAQWRKLGVAGEEENIGKGVAFCAHCDGPFF
HHHHHCCCCEEEEECCCCCEECCEEEEECCCCHHEECCCCCCCCCCCCEEEEEECCCCCC
KGKDIAVVGGGNSGIEAALDLAGIVKHVTVLEFADDLKADQVLINKAKEKANIDIITSAA
CCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCEEEEECCH
TQEIKATEGKVSSIIYQDRNSSENKELDVAGVFVQIGLIPNTGFVKGFVDLNRFGEIEID
HHHHHHCCCHHHEEEEECCCCCCCCCEEEEEEEEEEEEECCCCCEEEEHHHCCCCCCEEH
ERCRTDRKGIFACGDVTTVPFKQINIAMGEGSKAALSAFEYLVMQ
HHHCCCCCCEEEECCCCCCCCEEEEEEECCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ROOH; Proton; NADH [C]

Specific reaction: ROOH + Proton + NADH = an alcohol + H2O + NAD [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503; 1592833 [H]