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The map label for this gene is thcD [H]

Identifier: 93006794

GI number: 93006794

Start: 2429255

End: 2430895

Strand: Reverse

Name: thcD [H]

Synonym: Pcryo_1970

Alternate gene names: 93006794

Gene position: 2430895-2429255 (Counterclockwise)

Preceding gene: 93006796

Following gene: 93006793

Centisome position: 79.44

GC content: 46.37

Gene sequence:

>1641_bases
ATGAGTAAGGCAGCTACTATAAATACAGTCACTATAAACAAAGCCGACATTATCAGCGGCGGTATGAAATCGTACAAGCA
AGATGATGATGGTATCATTTTAATTACCCGTGACGACAGCACGTTTCGTGCTTTTGATGGCAAATGTCCGCATGCTGGAG
CAGATTTGGGTACAGGTTTGCGTTGTGGTAATCGCGTTATTTGTCCTTGGCATCATGCAGCTTTTGATAGCAGCGACGGC
GCATTACTGGAGCCAATCGCGACAACAGGCTTGACTCAATATCAAATCATAGATAATGGCGACAGCCTAACGGTTGATAC
CTCAGCCAAGATAGATAAGCAGGTTGAAAATGATAAGCTGACTGATACTCATACTATTATCGTTGGCGGCGGTGGAGCAG
GGTTTATGACTGCCCATCAGCTGCGCAATACTGGCTACGGCGGCAAGATTACCTTGGTGAGTGCCGAGGACAAAGCCCCT
TATAATCGTCCTTTATTATCCAAGGCTTTTTTGGCCGGTAATATGCCAGAAGAAAAGCTATTACTGGGCGGTACAGATTG
GGCAAGCAAACACGATATTGAACTGCGCTTAAACCAAACGGTCAGTGAAGTGTTGCTCAATGAGCGTAGTATCGTGATAA
CAGATGAAAAGGGTGATAGCAACAAGCTAACAGCGGAATTTTTAGTTGTGGCAACCGGTGCTGAGCCCAAGATTCCACCT
ATTAAAGGGGCAACTCTTGATGGTGTCTATACCTTGCGTAGTATGGATGATGCCAAATTAATCAAAGCCGCCAGTCATGA
TCAGCGCGTAGTCATCGTCGGTACGGGCTTTATCGGTATGGAAGTTGCGTCAGCATTGGCGCAATCGGGTACTACTGCTT
CTATCACTGTTATCGGTCAAGACCATCGCGTGATGGGCAATATCGTCTCAGAAACGGTCAGTAATGCGTTGATTACATTG
CATAAAGAAAATGGTGTTGAGTTCGTGTTTGATGCCAGCGTCGCTGAAATCATTGCAGCTGAAAATACTACTGATAATGG
CGAAGAGAATTTCTCACAAGTGTCTGGCGTCAAATTAGCCAATGGTGAGCAAATCGATGCTGATATCGTCATTTTAGGAA
CAGGTGTCGCACCGCGTATCGAGTTATTACAGGATACAAATCATCCAGATGGCGTAAAAGTCGATGAGCATTTACAACTG
CGCGATGGTGTCTATGCGTTGGGGGATATTGCCAAAGCGCCCAATCAACTGGGGCGTATGCGTATTGAGCACTGGCGGGT
TGCATTGCAACATGGCATGGTGACGGCTGCTGCTATCTTAAATGACGATAACGTCAAATCGCTTCAGGAGCGCATTCCTT
TCTTTTGGACGATGCAATATGGCAAAAGTTTGCGTTATAGTGGACATGCGGCGACGCCAGATCATGGCATTCTAATGGGT
TCGCCAGATAGTTTTAACTATATAGAATACTACTTCGATAACGAGGGTGAAAACACGTGTGCCAGTGCATCAAGCTCGCT
TGGACGTGATAAAGAGCTGGTGGCATTCTCTGAGTTATTACGCCAAGGTCATGCGCCAACACGGGCACAGATTAACGCAG
GGTTTGATATCATCGAGCAAGCGCAGGCGTTATCGCGCTAG

Upstream 100 bases:

>100_bases
TCAATATACAAAAGAACAGCATAAGTGAAGGAGGCTTGCTACTATCGATAAGTCTCGACACGCTTTAAACAGGAACCATT
ATAACCGTTAAGGATGACTT

Downstream 100 bases:

>100_bases
TAATGAAAACGGATAAATATTACTATTAAAGCTGAGCTATTTATTTAGGTCGGCTTTTTAATCTAGGATAGATACCACAT
AAAGATCTCACGTATTTTAT

Product: FAD-dependent pyridine nucleotide-disulfide oxidoreductase

Products: Oxidized Ferredoxin; NADH; cis-3-(carboxyethyl)-3,5-cyclohexadiene-1,2-diol; NAD [C]

Alternate protein names: NA

Number of amino acids: Translated: 546; Mature: 545

Protein sequence:

>546_residues
MSKAATINTVTINKADIISGGMKSYKQDDDGIILITRDDSTFRAFDGKCPHAGADLGTGLRCGNRVICPWHHAAFDSSDG
ALLEPIATTGLTQYQIIDNGDSLTVDTSAKIDKQVENDKLTDTHTIIVGGGGAGFMTAHQLRNTGYGGKITLVSAEDKAP
YNRPLLSKAFLAGNMPEEKLLLGGTDWASKHDIELRLNQTVSEVLLNERSIVITDEKGDSNKLTAEFLVVATGAEPKIPP
IKGATLDGVYTLRSMDDAKLIKAASHDQRVVIVGTGFIGMEVASALAQSGTTASITVIGQDHRVMGNIVSETVSNALITL
HKENGVEFVFDASVAEIIAAENTTDNGEENFSQVSGVKLANGEQIDADIVILGTGVAPRIELLQDTNHPDGVKVDEHLQL
RDGVYALGDIAKAPNQLGRMRIEHWRVALQHGMVTAAAILNDDNVKSLQERIPFFWTMQYGKSLRYSGHAATPDHGILMG
SPDSFNYIEYYFDNEGENTCASASSSLGRDKELVAFSELLRQGHAPTRAQINAGFDIIEQAQALSR

Sequences:

>Translated_546_residues
MSKAATINTVTINKADIISGGMKSYKQDDDGIILITRDDSTFRAFDGKCPHAGADLGTGLRCGNRVICPWHHAAFDSSDG
ALLEPIATTGLTQYQIIDNGDSLTVDTSAKIDKQVENDKLTDTHTIIVGGGGAGFMTAHQLRNTGYGGKITLVSAEDKAP
YNRPLLSKAFLAGNMPEEKLLLGGTDWASKHDIELRLNQTVSEVLLNERSIVITDEKGDSNKLTAEFLVVATGAEPKIPP
IKGATLDGVYTLRSMDDAKLIKAASHDQRVVIVGTGFIGMEVASALAQSGTTASITVIGQDHRVMGNIVSETVSNALITL
HKENGVEFVFDASVAEIIAAENTTDNGEENFSQVSGVKLANGEQIDADIVILGTGVAPRIELLQDTNHPDGVKVDEHLQL
RDGVYALGDIAKAPNQLGRMRIEHWRVALQHGMVTAAAILNDDNVKSLQERIPFFWTMQYGKSLRYSGHAATPDHGILMG
SPDSFNYIEYYFDNEGENTCASASSSLGRDKELVAFSELLRQGHAPTRAQINAGFDIIEQAQALSR
>Mature_545_residues
SKAATINTVTINKADIISGGMKSYKQDDDGIILITRDDSTFRAFDGKCPHAGADLGTGLRCGNRVICPWHHAAFDSSDGA
LLEPIATTGLTQYQIIDNGDSLTVDTSAKIDKQVENDKLTDTHTIIVGGGGAGFMTAHQLRNTGYGGKITLVSAEDKAPY
NRPLLSKAFLAGNMPEEKLLLGGTDWASKHDIELRLNQTVSEVLLNERSIVITDEKGDSNKLTAEFLVVATGAEPKIPPI
KGATLDGVYTLRSMDDAKLIKAASHDQRVVIVGTGFIGMEVASALAQSGTTASITVIGQDHRVMGNIVSETVSNALITLH
KENGVEFVFDASVAEIIAAENTTDNGEENFSQVSGVKLANGEQIDADIVILGTGVAPRIELLQDTNHPDGVKVDEHLQLR
DGVYALGDIAKAPNQLGRMRIEHWRVALQHGMVTAAAILNDDNVKSLQERIPFFWTMQYGKSLRYSGHAATPDHGILMGS
PDSFNYIEYYFDNEGENTCASASSSLGRDKELVAFSELLRQGHAPTRAQINAGFDIIEQAQALSR

Specific function: The degradation of the thiocarbamate herbicide EPTC by cytochrome CYP116 (thcB) requires the participation of a flavoprotein, rhodocoxin reductase, and an iron-sulfur protein, rhodocoxin, to mediate the transfer of electrons from NADH to P450 for oxygen a

COG id: COG0446

COG function: function code R; Uncharacterized NAD(FAD)-dependent dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAD-dependent oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI226437568, Length=524, Percent_Identity=27.8625954198473, Blast_Score=207, Evalue=2e-53,
Organism=Homo sapiens, GI65787454, Length=524, Percent_Identity=27.8625954198473, Blast_Score=207, Evalue=2e-53,
Organism=Homo sapiens, GI21389617, Length=522, Percent_Identity=27.7777777777778, Blast_Score=205, Evalue=9e-53,
Organism=Homo sapiens, GI4757732, Length=320, Percent_Identity=25, Blast_Score=76, Evalue=1e-13,
Organism=Homo sapiens, GI22202629, Length=320, Percent_Identity=25, Blast_Score=75, Evalue=1e-13,
Organism=Escherichia coli, GI1788892, Length=380, Percent_Identity=28.4210526315789, Blast_Score=122, Evalue=7e-29,
Organism=Escherichia coli, GI1789065, Length=296, Percent_Identity=26.3513513513513, Blast_Score=82, Evalue=6e-17,
Organism=Caenorhabditis elegans, GI17559934, Length=522, Percent_Identity=25.8620689655172, Blast_Score=156, Evalue=3e-38,
Organism=Drosophila melanogaster, GI24639257, Length=541, Percent_Identity=30.6839186691312, Blast_Score=207, Evalue=2e-53,
Organism=Drosophila melanogaster, GI281359715, Length=541, Percent_Identity=30.6839186691312, Blast_Score=207, Evalue=2e-53,
Organism=Drosophila melanogaster, GI281359713, Length=541, Percent_Identity=30.6839186691312, Blast_Score=207, Evalue=2e-53,
Organism=Drosophila melanogaster, GI24639250, Length=541, Percent_Identity=30.6839186691312, Blast_Score=207, Evalue=2e-53,
Organism=Drosophila melanogaster, GI18543267, Length=541, Percent_Identity=30.6839186691312, Blast_Score=207, Evalue=2e-53,
Organism=Drosophila melanogaster, GI24639252, Length=541, Percent_Identity=30.6839186691312, Blast_Score=207, Evalue=2e-53,
Organism=Drosophila melanogaster, GI24585130, Length=542, Percent_Identity=27.4907749077491, Blast_Score=187, Evalue=2e-47,
Organism=Drosophila melanogaster, GI24581020, Length=338, Percent_Identity=24.2603550295858, Blast_Score=82, Evalue=8e-16,
Organism=Drosophila melanogaster, GI28573993, Length=338, Percent_Identity=24.2603550295858, Blast_Score=82, Evalue=9e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR004099
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: 1.18.1.3 [C]

Molecular weight: Translated: 58718; Mature: 58586

Theoretical pI: Translated: 4.88; Mature: 4.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKAATINTVTINKADIISGGMKSYKQDDDGIILITRDDSTFRAFDGKCPHAGADLGTGL
CCCCEEEEEEEECCHHHHHCHHHHCCCCCCCEEEEECCCCCEEECCCCCCCCCCCCCCCC
RCGNRVICPWHHAAFDSSDGALLEPIATTGLTQYQIIDNGDSLTVDTSAKIDKQVENDKL
CCCCEEEECCHHHCCCCCCCCEECHHHHCCCEEEEEEECCCEEEEECCHHHHHHHCCCCC
TDTHTIIVGGGGAGFMTAHQLRNTGYGGKITLVSAEDKAPYNRPLLSKAFLAGNMPEEKL
CCEEEEEEECCCCCHHHHHHHHCCCCCCEEEEEECCCCCCCCCCHHHHHHHCCCCCHHEE
LLGGTDWASKHDIELRLNQTVSEVLLNERSIVITDEKGDSNKLTAEFLVVATGAEPKIPP
EECCCCCCCCCCEEEEEHHHHHHHHHCCCEEEEECCCCCCCCEEEEEEEEEECCCCCCCC
IKGATLDGVYTLRSMDDAKLIKAASHDQRVVIVGTGFIGMEVASALAQSGTTASITVIGQ
CCCCCCCCEEEECCCCHHHHHHHCCCCCEEEEEECCHHHHHHHHHHHHCCCEEEEEEEEC
DHRVMGNIVSETVSNALITLHKENGVEFVFDASVAEIIAAENTTDNGEENFSQVSGVKLA
CCHHHHHHHHHHHCCEEEEEECCCCEEEEECCCHHHHEEECCCCCCCHHHHHHHCCEEEC
NGEQIDADIVILGTGVAPRIELLQDTNHPDGVKVDEHLQLRDGVYALGDIAKAPNQLGRM
CCCCCCCEEEEEECCCCCHHHHHHCCCCCCCCCCCCCEECCCCCEEHHHHHHCHHHHHHH
RIEHWRVALQHGMVTAAAILNDDNVKSLQERIPFFWTMQYGKSLRYSGHAATPDHGILMG
HHHHHHHHHHCCCEEEEEEECCCCHHHHHHHCCEEEEEECCCCEECCCCCCCCCCCEEEC
SPDSFNYIEYYFDNEGENTCASASSSLGRDKELVAFSELLRQGHAPTRAQINAGFDIIEQ
CCCCCCEEEEEECCCCCCHHHHHHHHCCCCHHHHHHHHHHHHCCCCCEEEECCCHHHHHH
AQALSR
HHHHCC
>Mature Secondary Structure 
SKAATINTVTINKADIISGGMKSYKQDDDGIILITRDDSTFRAFDGKCPHAGADLGTGL
CCCEEEEEEEECCHHHHHCHHHHCCCCCCCEEEEECCCCCEEECCCCCCCCCCCCCCCC
RCGNRVICPWHHAAFDSSDGALLEPIATTGLTQYQIIDNGDSLTVDTSAKIDKQVENDKL
CCCCEEEECCHHHCCCCCCCCEECHHHHCCCEEEEEEECCCEEEEECCHHHHHHHCCCCC
TDTHTIIVGGGGAGFMTAHQLRNTGYGGKITLVSAEDKAPYNRPLLSKAFLAGNMPEEKL
CCEEEEEEECCCCCHHHHHHHHCCCCCCEEEEEECCCCCCCCCCHHHHHHHCCCCCHHEE
LLGGTDWASKHDIELRLNQTVSEVLLNERSIVITDEKGDSNKLTAEFLVVATGAEPKIPP
EECCCCCCCCCCEEEEEHHHHHHHHHCCCEEEEECCCCCCCCEEEEEEEEEECCCCCCCC
IKGATLDGVYTLRSMDDAKLIKAASHDQRVVIVGTGFIGMEVASALAQSGTTASITVIGQ
CCCCCCCCEEEECCCCHHHHHHHCCCCCEEEEEECCHHHHHHHHHHHHCCCEEEEEEEEC
DHRVMGNIVSETVSNALITLHKENGVEFVFDASVAEIIAAENTTDNGEENFSQVSGVKLA
CCHHHHHHHHHHHCCEEEEEECCCCEEEEECCCHHHHEEECCCCCCCHHHHHHHCCEEEC
NGEQIDADIVILGTGVAPRIELLQDTNHPDGVKVDEHLQLRDGVYALGDIAKAPNQLGRM
CCCCCCCEEEEEECCCCCHHHHHHCCCCCCCCCCCCCEECCCCCEEHHHHHHCHHHHHHH
RIEHWRVALQHGMVTAAAILNDDNVKSLQERIPFFWTMQYGKSLRYSGHAATPDHGILMG
HHHHHHHHHHCCCEEEEEEECCCCHHHHHHHCCEEEEEECCCCEECCCCCCCCCCCEEEC
SPDSFNYIEYYFDNEGENTCASASSSLGRDKELVAFSELLRQGHAPTRAQINAGFDIIEQ
CCCCCCEEEEEECCCCCCHHHHHHHHCCCCHHHHHHHHHHHHCCCCCEEEECCCHHHHHH
AQALSR
HHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Reduced Ferredoxin; NAD(+); 3-phenylpropionate; NADH; Proton; O2 [C]

Specific reaction: Reduced Ferredoxin + NAD(+) = Oxidized Ferredoxin + NADH. 3-phenylpropionate + NADH + Proton + O2 = cis-3-(carboxyethyl)-3,5-cyclohexadiene-1,2-diol + NAD [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7836301 [H]