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The map label for this gene is gdhA [H]

Identifier: 93006759

GI number: 93006759

Start: 2388428

End: 2389774

Strand: Reverse

Name: gdhA [H]

Synonym: Pcryo_1935

Alternate gene names: 93006759

Gene position: 2389774-2388428 (Counterclockwise)

Preceding gene: 93006760

Following gene: 93006753

Centisome position: 78.1

GC content: 44.69

Gene sequence:

>1347_bases
ATGAGTATCAGTCAGGCAATCGAAAAAATCGAAGCACGTTATGCCCATCAACCTGAGTTTATTCAGGCAGTAAAAGAAGT
TGCAATCACGATTGCGCCACTATATGACTCTCATCCTGAATATGATGCTTTCAAAGTTTTTGAGCGCTTGGTTGAGCCTG
ACCGCGTGATGGGTTTCCGTATCAACTGGGAAAACGATAAAGGTGTAGTACAGGTCAATCGCGGCTGGCGTGTCCAATTT
AGTAACGCGTTAGGTCCTTATAAGGGTGGTATTAGATTTCACCCAACCGTCAATCAATCGGTCTTAAAATTCTTAGGATT
TGAGCAAATTTTTAAAAATGCCTTGACCGGTTTGCCAATGGGCGGCGCTAAAGGTGGTTCTGATTTTGATCCAAAGGGCA
AGTCAGACAACGAAATCCGTCGTTTTTGCTATGCTTTCATGCGTGAGCTGCATCCCTATATCAATAAAGATACGGATGTA
CCGGCAGGTGATATCGGCGTTAGCGGACGCGAAATCAGCTATATGTTTGCCATGTATAAAAACCTGACCCGCGAATACGG
CGGTGTATTGACTGGTAAAGGCGTAGGGTTTGGTGGCAGTTTGATGCGTACGGAAGCGACGGGCTATGGCGCGGTTTACT
TCTTAGAAAATATGCTAACGGCTCAAAATGAGCGCATAGATGGTAAGCGAGTACTGGTTTCAGGCGCGGGTAACGTCTCT
CTACATGCCGCTGAAAAAGCTCATATGCTGGGCGGTGTTGTGGTCACCGTATCTGATTCCCAAGGTACCTTGTATGATGG
GGCGGGTCTAAATCAAGAAAAAATAAACTGGTTAAAAGCGCAAAAAGCAAAAAGCCAACCATTAGCAGATTACGTAGCTG
TCTATGGCGGTGAATGGTTCGCTGGTCAAAAACCTTGGCATATCAAAGGCGATATCGCTATTCCATCAGCCACGCAAAAC
GAAGTGAATGAAGACGACGCTAAGCTGATGGTTGAGAACGGCATTAAGTATGTGGTTGAAGGCGCTAATATGCCATTGAC
TGCAGAAGCAATTGATCATATCCGCTTGCACCGTGTGCACTATGCTCCAGGTAAAGCGGCAAATGCAGGCGGCGTAGCAG
TATCTGGTCTTGAGATGTCACAGAACTCAGTACGTCAGTACAAAACTTTTGAAGAGATTGATGCACGTTTAAAAGTTATT
ATGAAAAATATTCATGACGGCGCTGCTGAAGCCTCAGAAAAATACCATCAAACTGATAAAGGTTATATCGATTATATGAC
TGGCGCTAATATCGTCGGCTTTAAACGTGTTGCCGATGCCTTAGTTGCTTATGGTATCTTGAACTAA

Upstream 100 bases:

>100_bases
TCAATCCTACAACTGTGTTCACCATCCTATCATTTTCGGGTATGCGATAGTGCGCCGGTGTAAATCAGCACGATTACAAT
TTCTACCCTAAGGAACCTAT

Downstream 100 bases:

>100_bases
GAGCGGTTTATAGATCATACTACAGTCGATTCAATTTAAAAAGAATACAGTACTGTTGGGATAAATTTTGCGAAGCCTCT
GGAGTGCGAAGCGCACAGCA

Product: glutamate dehydrogenase

Products: NA

Alternate protein names: NADP-GDH [H]

Number of amino acids: Translated: 448; Mature: 447

Protein sequence:

>448_residues
MSISQAIEKIEARYAHQPEFIQAVKEVAITIAPLYDSHPEYDAFKVFERLVEPDRVMGFRINWENDKGVVQVNRGWRVQF
SNALGPYKGGIRFHPTVNQSVLKFLGFEQIFKNALTGLPMGGAKGGSDFDPKGKSDNEIRRFCYAFMRELHPYINKDTDV
PAGDIGVSGREISYMFAMYKNLTREYGGVLTGKGVGFGGSLMRTEATGYGAVYFLENMLTAQNERIDGKRVLVSGAGNVS
LHAAEKAHMLGGVVVTVSDSQGTLYDGAGLNQEKINWLKAQKAKSQPLADYVAVYGGEWFAGQKPWHIKGDIAIPSATQN
EVNEDDAKLMVENGIKYVVEGANMPLTAEAIDHIRLHRVHYAPGKAANAGGVAVSGLEMSQNSVRQYKTFEEIDARLKVI
MKNIHDGAAEASEKYHQTDKGYIDYMTGANIVGFKRVADALVAYGILN

Sequences:

>Translated_448_residues
MSISQAIEKIEARYAHQPEFIQAVKEVAITIAPLYDSHPEYDAFKVFERLVEPDRVMGFRINWENDKGVVQVNRGWRVQF
SNALGPYKGGIRFHPTVNQSVLKFLGFEQIFKNALTGLPMGGAKGGSDFDPKGKSDNEIRRFCYAFMRELHPYINKDTDV
PAGDIGVSGREISYMFAMYKNLTREYGGVLTGKGVGFGGSLMRTEATGYGAVYFLENMLTAQNERIDGKRVLVSGAGNVS
LHAAEKAHMLGGVVVTVSDSQGTLYDGAGLNQEKINWLKAQKAKSQPLADYVAVYGGEWFAGQKPWHIKGDIAIPSATQN
EVNEDDAKLMVENGIKYVVEGANMPLTAEAIDHIRLHRVHYAPGKAANAGGVAVSGLEMSQNSVRQYKTFEEIDARLKVI
MKNIHDGAAEASEKYHQTDKGYIDYMTGANIVGFKRVADALVAYGILN
>Mature_447_residues
SISQAIEKIEARYAHQPEFIQAVKEVAITIAPLYDSHPEYDAFKVFERLVEPDRVMGFRINWENDKGVVQVNRGWRVQFS
NALGPYKGGIRFHPTVNQSVLKFLGFEQIFKNALTGLPMGGAKGGSDFDPKGKSDNEIRRFCYAFMRELHPYINKDTDVP
AGDIGVSGREISYMFAMYKNLTREYGGVLTGKGVGFGGSLMRTEATGYGAVYFLENMLTAQNERIDGKRVLVSGAGNVSL
HAAEKAHMLGGVVVTVSDSQGTLYDGAGLNQEKINWLKAQKAKSQPLADYVAVYGGEWFAGQKPWHIKGDIAIPSATQNE
VNEDDAKLMVENGIKYVVEGANMPLTAEAIDHIRLHRVHYAPGKAANAGGVAVSGLEMSQNSVRQYKTFEEIDARLKVIM
KNIHDGAAEASEKYHQTDKGYIDYMTGANIVGFKRVADALVAYGILN

Specific function: Unknown

COG id: COG0334

COG function: function code E; Glutamate dehydrogenase/leucine dehydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Glu/Leu/Phe/Val dehydrogenases family [H]

Homologues:

Organism=Homo sapiens, GI31377775, Length=355, Percent_Identity=30.9859154929577, Blast_Score=140, Evalue=3e-33,
Organism=Homo sapiens, GI4885281, Length=355, Percent_Identity=30.9859154929577, Blast_Score=138, Evalue=9e-33,
Organism=Escherichia coli, GI1788059, Length=447, Percent_Identity=52.7964205816555, Blast_Score=469, Evalue=1e-133,
Organism=Caenorhabditis elegans, GI17544676, Length=349, Percent_Identity=29.2263610315186, Blast_Score=120, Evalue=1e-27,
Organism=Saccharomyces cerevisiae, GI6324951, Length=451, Percent_Identity=49.2239467849224, Blast_Score=399, Evalue=1e-112,
Organism=Saccharomyces cerevisiae, GI6319256, Length=453, Percent_Identity=49.0066225165563, Blast_Score=399, Evalue=1e-112,
Organism=Drosophila melanogaster, GI45549226, Length=349, Percent_Identity=30.6590257879656, Blast_Score=130, Evalue=2e-30,
Organism=Drosophila melanogaster, GI45553475, Length=349, Percent_Identity=30.6590257879656, Blast_Score=130, Evalue=2e-30,
Organism=Drosophila melanogaster, GI24649283, Length=345, Percent_Identity=30.1449275362319, Blast_Score=108, Evalue=7e-24,
Organism=Drosophila melanogaster, GI24649460, Length=287, Percent_Identity=30.6620209059233, Blast_Score=104, Evalue=1e-22,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 9362 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006095
- InterPro:   IPR006096
- InterPro:   IPR006097
- InterPro:   IPR014362
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00208 ELFV_dehydrog; PF02812 ELFV_dehydrog_N [H]

EC number: =1.4.1.4 [H]

Molecular weight: Translated: 49306; Mature: 49174

Theoretical pI: Translated: 7.18; Mature: 7.18

Prosite motif: PS00074 GLFV_DEHYDROGENASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSISQAIEKIEARYAHQPEFIQAVKEVAITIAPLYDSHPEYDAFKVFERLVEPDRVMGFR
CCHHHHHHHHHHHHCCCCHHHHHHHHHHEEEEECCCCCCCHHHHHHHHHHCCCCCEEEEE
INWENDKGVVQVNRGWRVQFSNALGPYKGGIRFHPTVNQSVLKFLGFEQIFKNALTGLPM
EEECCCCCEEEECCCEEEEECCCCCCCCCCEEECCCCCHHHHHHHCHHHHHHHHHCCCCC
GGAKGGSDFDPKGKSDNEIRRFCYAFMRELHPYINKDTDVPAGDIGVSGREISYMFAMYK
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHH
NLTREYGGVLTGKGVGFGGSLMRTEATGYGAVYFLENMLTAQNERIDGKRVLVSGAGNVS
HHHHHHCCEEECCCCCCCCCEEEECCCCCCHHHHHHHHHHHCCCCCCCCEEEEECCCCEE
LHAAEKAHMLGGVVVTVSDSQGTLYDGAGLNQEKINWLKAQKAKSQPLADYVAVYGGEWF
EEHHHHHHHHCCEEEEEECCCCCEECCCCCCHHHHHHHHHHHHCCCCHHHHHHHHCCCCC
AGQKPWHIKGDIAIPSATQNEVNEDDAKLMVENGIKYVVEGANMPLTAEAIDHIRLHRVH
CCCCCEEEECCEECCCCCCCCCCCCHHHEEHHCCCEEEEECCCCCEEHHHHHHHHHEEEE
YAPGKAANAGGVAVSGLEMSQNSVRQYKTFEEIDARLKVIMKNIHDGAAEASEKYHQTDK
ECCCCCCCCCCEEEECCHHCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCC
GYIDYMTGANIVGFKRVADALVAYGILN
CEEEEECCCCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SISQAIEKIEARYAHQPEFIQAVKEVAITIAPLYDSHPEYDAFKVFERLVEPDRVMGFR
CHHHHHHHHHHHHCCCCHHHHHHHHHHEEEEECCCCCCCHHHHHHHHHHCCCCCEEEEE
INWENDKGVVQVNRGWRVQFSNALGPYKGGIRFHPTVNQSVLKFLGFEQIFKNALTGLPM
EEECCCCCEEEECCCEEEEECCCCCCCCCCEEECCCCCHHHHHHHCHHHHHHHHHCCCCC
GGAKGGSDFDPKGKSDNEIRRFCYAFMRELHPYINKDTDVPAGDIGVSGREISYMFAMYK
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHH
NLTREYGGVLTGKGVGFGGSLMRTEATGYGAVYFLENMLTAQNERIDGKRVLVSGAGNVS
HHHHHHCCEEECCCCCCCCCEEEECCCCCCHHHHHHHHHHHCCCCCCCCEEEEECCCCEE
LHAAEKAHMLGGVVVTVSDSQGTLYDGAGLNQEKINWLKAQKAKSQPLADYVAVYGGEWF
EEHHHHHHHHCCEEEEEECCCCCEECCCCCCHHHHHHHHHHHHCCCCHHHHHHHHCCCCC
AGQKPWHIKGDIAIPSATQNEVNEDDAKLMVENGIKYVVEGANMPLTAEAIDHIRLHRVH
CCCCCEEEECCEECCCCCCCCCCCCHHHEEHHCCCEEEEECCCCCEEHHHHHHHHHEEEE
YAPGKAANAGGVAVSGLEMSQNSVRQYKTFEEIDARLKVIMKNIHDGAAEASEKYHQTDK
ECCCCCCCCCCEEEECCHHCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCC
GYIDYMTGANIVGFKRVADALVAYGILN
CEEEEECCCCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10601858 [H]