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The map label for this gene is rutR [H]

Identifier: 93006738

GI number: 93006738

Start: 2365108

End: 2365773

Strand: Reverse

Name: rutR [H]

Synonym: Pcryo_1914

Alternate gene names: 93006738

Gene position: 2365773-2365108 (Counterclockwise)

Preceding gene: 93006750

Following gene: 93006736

Centisome position: 77.32

GC content: 45.95

Gene sequence:

>666_bases
ATGACTCAAATTTCTAAAGCAAAAGCTTCATCCGCCTCTGAGAGCGTGGTTCATCGCAATCAGCATGATATTCGTGCCCA
AAACCAAGCGGTGATTTTGACTGCCGCTGAAGAAGAGTTTGTGCTGAAAAGCTATCGCGGTGCCACCATGCAAGGTATCG
CTGATCGAGCAGGGCTGCCAAAAGCCAATGTGCATTACTATTTTAAAAGTAAGACCAATCTGTACAAAGTAGTTTTGCGT
GCCATCATTCAAGAGTGGAATGATGGCTTGGTGACGATGACGGTAGACAGCGATCCAAGAACCGTTATCGAAAAGTTCGT
CCGCACCAAATTGCATCAAGCATTTGCAAACCCAAATCGCCATAAGCTATTTGCGTTAGAGGTCATCGGCGGTGCCCCGC
ATTTACATGATTTTATGTCGACTGCGATGAAAGATTGGGCACTGGATAAGACACAAGTCATGAAGATATGGCATGAGCAA
GGCAAAATTGCCATTGCTGACCCTTTACAGCTATTGATTTTGATTTGGGCAACCACGCAGCGCTATGCAGAGTTTGAAAC
AGAAATCGTCGGCTTAATGCAAAAGAGCGCGTATGATGAGACAGACGAGGCACGCGCTGCTGACTTCTTAATACCTTTTA
TACTACGAGGTTGCGGACTCGAGTAG

Upstream 100 bases:

>100_bases
TTACTAGATATGCTAATAATCGGTGTGTTTATTTACAGTTTATCTTAAAGTCACGCTAGAATATGGTACTTACTGATATA
AATGTCTAATGGTGCAACCT

Downstream 100 bases:

>100_bases
GGGTAGATTGGAACAACTGCTATTAATGAATGGCTTGACGCATAAAGGATTGCTTTAACGATATCGCTGCTAATTAGCGC
TTTATCATTAAGGCAATCCT

Product: TetR family transcriptional regulator

Products: NA

Alternate protein names: Rut operon repressor [H]

Number of amino acids: Translated: 221; Mature: 220

Protein sequence:

>221_residues
MTQISKAKASSASESVVHRNQHDIRAQNQAVILTAAEEEFVLKSYRGATMQGIADRAGLPKANVHYYFKSKTNLYKVVLR
AIIQEWNDGLVTMTVDSDPRTVIEKFVRTKLHQAFANPNRHKLFALEVIGGAPHLHDFMSTAMKDWALDKTQVMKIWHEQ
GKIAIADPLQLLILIWATTQRYAEFETEIVGLMQKSAYDETDEARAADFLIPFILRGCGLE

Sequences:

>Translated_221_residues
MTQISKAKASSASESVVHRNQHDIRAQNQAVILTAAEEEFVLKSYRGATMQGIADRAGLPKANVHYYFKSKTNLYKVVLR
AIIQEWNDGLVTMTVDSDPRTVIEKFVRTKLHQAFANPNRHKLFALEVIGGAPHLHDFMSTAMKDWALDKTQVMKIWHEQ
GKIAIADPLQLLILIWATTQRYAEFETEIVGLMQKSAYDETDEARAADFLIPFILRGCGLE
>Mature_220_residues
TQISKAKASSASESVVHRNQHDIRAQNQAVILTAAEEEFVLKSYRGATMQGIADRAGLPKANVHYYFKSKTNLYKVVLRA
IIQEWNDGLVTMTVDSDPRTVIEKFVRTKLHQAFANPNRHKLFALEVIGGAPHLHDFMSTAMKDWALDKTQVMKIWHEQG
KIAIADPLQLLILIWATTQRYAEFETEIVGLMQKSAYDETDEARAADFLIPFILRGCGLE

Specific function: Master transcription regulator which represses the degradation of pyrimidines (rutABCDEFG) and purines (gcl operon) for maintenance of metabolic balance between pyrimidines and purines. It also regulates the synthesis of pyrimidine nucleotides and arginin

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH tetR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787249, Length=183, Percent_Identity=33.3333333333333, Blast_Score=110, Evalue=8e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR015893
- InterPro:   IPR011075
- InterPro:   IPR019915
- InterPro:   IPR001647
- InterPro:   IPR013573 [H]

Pfam domain/function: PF08362 TetR_C_3; PF00440 TetR_N [H]

EC number: NA

Molecular weight: Translated: 24987; Mature: 24856

Theoretical pI: Translated: 7.82; Mature: 7.82

Prosite motif: PS50977 HTH_TETR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQISKAKASSASESVVHRNQHDIRAQNQAVILTAAEEEFVLKSYRGATMQGIADRAGLP
CCCCCHHHHCCHHHHHHHCCCHHHCCCCCEEEEEECCHHHHHHHHCCCHHHHHHHHCCCC
KANVHYYFKSKTNLYKVVLRAIIQEWNDGLVTMTVDSDPRTVIEKFVRTKLHQAFANPNR
CCCEEEEECCCCHHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHHHHCCCCC
HKLFALEVIGGAPHLHDFMSTAMKDWALDKTQVMKIWHEQGKIAIADPLQLLILIWATTQ
CEEEEEEEECCCCHHHHHHHHHHHHHCCCHHHHHHHHHHCCCEEEECHHHHHHHHHHHHH
RYAEFETEIVGLMQKSAYDETDEARAADFLIPFILRGCGLE
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TQISKAKASSASESVVHRNQHDIRAQNQAVILTAAEEEFVLKSYRGATMQGIADRAGLP
CCCCHHHHCCHHHHHHHCCCHHHCCCCCEEEEEECCHHHHHHHHCCCHHHHHHHHCCCC
KANVHYYFKSKTNLYKVVLRAIIQEWNDGLVTMTVDSDPRTVIEKFVRTKLHQAFANPNR
CCCEEEEECCCCHHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHHHHCCCCC
HKLFALEVIGGAPHLHDFMSTAMKDWALDKTQVMKIWHEQGKIAIADPLQLLILIWATTQ
CEEEEEEEECCCCHHHHHHHHHHHHHCCCHHHHHHHHHHCCCEEEECHHHHHHHHHHHHH
RYAEFETEIVGLMQKSAYDETDEARAADFLIPFILRGCGLE
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]