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The map label for this gene is eno [H]

Identifier: 93006694

GI number: 93006694

Start: 2308977

End: 2310293

Strand: Reverse

Name: eno [H]

Synonym: Pcryo_1870

Alternate gene names: 93006694

Gene position: 2310293-2308977 (Counterclockwise)

Preceding gene: 93006695

Following gene: 93006693

Centisome position: 75.5

GC content: 45.56

Gene sequence:

>1317_bases
ATGTACGCTGAAGAAACCAACAACGTCACCGCAATTAAAGACATTCGCGCTCGTGAGATTTTAGACTCACGTGGTAACCC
AACGATTGAAGCTGATGTGATTTTAGCTGATGGCACTATTGGCCGTGCAGCTGCGCCAAGCGGCGCATCGACTGGCTCAC
GTGAGGCGCTTGAGCTACGTGATGGCGATCAAAGCCGCTACATGGGTAAAGGCGTCAAAAAGGCAGTAGCTAACGTCAAT
AGCCAAATCCGCAGTGCTTTGATGGACAAAGATGTTACTGCTCAGCAAGCTATTGATGATGCAATGATTGCGCTAGATGG
TACAGAAAACAAAGACAATCTTGGTGCCAATGCCATACTTGCGGTTTCACTTGCGGTTGCTAAAGCGGCTGCTAAGTCAC
AGAGTCTGCCATTACATCAATATATCGCTGATCTGCGTAACCAGACGTCGCTTACGATGCCTGTACCGATGATGAATATC
ATTAATGGTGGTGAGCACGCGGACAATACCGTCGATATTCAAGAGTTTATGATTGAGCCGGTTGGTTTTAGCAGCTTCTC
AGAAGCATTGCGTGCTGGCACCGAAATCTTTCATAGCTTAAAGTCTGTTCTAAAATCACAAGGCTTGAATACTGCTGTTG
GTGATGAAGGCGGCTTTGCGCCAAATCTTCGCAGCAACGAAGAAGCCATTACAGTTATCATGCAAGCGATTGAGCAAGTA
GGCTACACAGCTGGTAAAGATATTCACTTAGCTTTAGATTGTGCTGCTACTGAGTTCTATAAAGATGGCAAATACGTCTT
GGCAGGCGAGGGCAACAAATCCTTTGATAGCCAAGGGTTTTCAGATTATTTAGTGGGTCTTGCGCGTCAATATCCTATTA
TCTCTATCGAAGATGGTCTTGATGAGTCAGATTGGGATGGCTGGAAATACCTAACTGAGCAAATCGGTGATAAGGTACAG
TTGGTCGGTGATGATTTGTTTGTCACCAACCCTGCTATCTTGCAAGAAGGGATTGATAAGAAAATTGCCAATGCGATTTT
GATTAAGTTTAACCAAATCGGCACCTTGTCAGAAACGCTAGATGCGATTTATCTGGCGAAGAAAAACGGTTATGCGACGG
TTATTTCGCATCGTTCAGGCGAAACGGAAGACAGCACCATTGCTGATTTAGCGGTCGGTACTGCGGCTGGTCAAATCAAA
ACTGGCTCACTATGCCGCTCAGATCGCGTTGCAAAATACAATCAACTACTACGTATTGAGCAGCAAGTACGTGCAAGCTA
TCGTGGTCGCGAAGAGTTTATCGGATTACGTGGTTAA

Upstream 100 bases:

>100_bases
TTGCGCATTACTATACGATATTCTGCTTTATCTTTTTTGTATTCTAGTTGCCGTTATTTATCTCATAAACTTGGCATGAC
CAAAGGAATTAACAGACATT

Downstream 100 bases:

>100_bases
TAAGCCAAAATTGTCGCTTACGCTGGGCGATATATAAATATATCGCTCAGCAAGTTTATATTCTCTTTATATTGTTTGGC
GTTATTATATTATGAAATAC

Product: phosphopyruvate hydratase

Products: NA

Alternate protein names: 2-phospho-D-glycerate hydro-lyase; 2-phosphoglycerate dehydratase [H]

Number of amino acids: Translated: 438; Mature: 438

Protein sequence:

>438_residues
MYAEETNNVTAIKDIRAREILDSRGNPTIEADVILADGTIGRAAAPSGASTGSREALELRDGDQSRYMGKGVKKAVANVN
SQIRSALMDKDVTAQQAIDDAMIALDGTENKDNLGANAILAVSLAVAKAAAKSQSLPLHQYIADLRNQTSLTMPVPMMNI
INGGEHADNTVDIQEFMIEPVGFSSFSEALRAGTEIFHSLKSVLKSQGLNTAVGDEGGFAPNLRSNEEAITVIMQAIEQV
GYTAGKDIHLALDCAATEFYKDGKYVLAGEGNKSFDSQGFSDYLVGLARQYPIISIEDGLDESDWDGWKYLTEQIGDKVQ
LVGDDLFVTNPAILQEGIDKKIANAILIKFNQIGTLSETLDAIYLAKKNGYATVISHRSGETEDSTIADLAVGTAAGQIK
TGSLCRSDRVAKYNQLLRIEQQVRASYRGREEFIGLRG

Sequences:

>Translated_438_residues
MYAEETNNVTAIKDIRAREILDSRGNPTIEADVILADGTIGRAAAPSGASTGSREALELRDGDQSRYMGKGVKKAVANVN
SQIRSALMDKDVTAQQAIDDAMIALDGTENKDNLGANAILAVSLAVAKAAAKSQSLPLHQYIADLRNQTSLTMPVPMMNI
INGGEHADNTVDIQEFMIEPVGFSSFSEALRAGTEIFHSLKSVLKSQGLNTAVGDEGGFAPNLRSNEEAITVIMQAIEQV
GYTAGKDIHLALDCAATEFYKDGKYVLAGEGNKSFDSQGFSDYLVGLARQYPIISIEDGLDESDWDGWKYLTEQIGDKVQ
LVGDDLFVTNPAILQEGIDKKIANAILIKFNQIGTLSETLDAIYLAKKNGYATVISHRSGETEDSTIADLAVGTAAGQIK
TGSLCRSDRVAKYNQLLRIEQQVRASYRGREEFIGLRG
>Mature_438_residues
MYAEETNNVTAIKDIRAREILDSRGNPTIEADVILADGTIGRAAAPSGASTGSREALELRDGDQSRYMGKGVKKAVANVN
SQIRSALMDKDVTAQQAIDDAMIALDGTENKDNLGANAILAVSLAVAKAAAKSQSLPLHQYIADLRNQTSLTMPVPMMNI
INGGEHADNTVDIQEFMIEPVGFSSFSEALRAGTEIFHSLKSVLKSQGLNTAVGDEGGFAPNLRSNEEAITVIMQAIEQV
GYTAGKDIHLALDCAATEFYKDGKYVLAGEGNKSFDSQGFSDYLVGLARQYPIISIEDGLDESDWDGWKYLTEQIGDKVQ
LVGDDLFVTNPAILQEGIDKKIANAILIKFNQIGTLSETLDAIYLAKKNGYATVISHRSGETEDSTIADLAVGTAAGQIK
TGSLCRSDRVAKYNQLLRIEQQVRASYRGREEFIGLRG

Specific function: Catalyzes the reversible conversion of 2- phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis [H]

COG id: COG0148

COG function: function code G; Enolase

Gene ontology:

Cell location: Cytoplasm. Secreted. Cell surface. Note=Fractions of enolase are present in both the cytoplasm and on the cell surface. The export of enolase possibly depends on the covalent binding to the substrate; once secreted, it remains attached to the bacterial ce

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enolase family [H]

Homologues:

Organism=Homo sapiens, GI5803011, Length=427, Percent_Identity=51.7564402810305, Blast_Score=427, Evalue=1e-120,
Organism=Homo sapiens, GI4503571, Length=430, Percent_Identity=50.2325581395349, Blast_Score=425, Evalue=1e-119,
Organism=Homo sapiens, GI301897477, Length=431, Percent_Identity=50.3480278422274, Blast_Score=423, Evalue=1e-118,
Organism=Homo sapiens, GI301897469, Length=431, Percent_Identity=50.3480278422274, Blast_Score=423, Evalue=1e-118,
Organism=Homo sapiens, GI301897479, Length=429, Percent_Identity=45.6876456876457, Blast_Score=366, Evalue=1e-101,
Organism=Homo sapiens, GI169201331, Length=341, Percent_Identity=26.9794721407625, Blast_Score=115, Evalue=1e-25,
Organism=Homo sapiens, GI169201757, Length=341, Percent_Identity=26.9794721407625, Blast_Score=115, Evalue=1e-25,
Organism=Homo sapiens, GI239744207, Length=341, Percent_Identity=26.9794721407625, Blast_Score=115, Evalue=1e-25,
Organism=Homo sapiens, GI310129182, Length=208, Percent_Identity=24.5192307692308, Blast_Score=69, Evalue=1e-11,
Organism=Homo sapiens, GI310110045, Length=208, Percent_Identity=24.5192307692308, Blast_Score=69, Evalue=1e-11,
Organism=Homo sapiens, GI310120572, Length=208, Percent_Identity=24.5192307692308, Blast_Score=69, Evalue=1e-11,
Organism=Escherichia coli, GI1789141, Length=430, Percent_Identity=67.906976744186, Blast_Score=580, Evalue=1e-167,
Organism=Caenorhabditis elegans, GI17536383, Length=423, Percent_Identity=54.6099290780142, Blast_Score=451, Evalue=1e-127,
Organism=Caenorhabditis elegans, GI71995829, Length=423, Percent_Identity=54.6099290780142, Blast_Score=451, Evalue=1e-127,
Organism=Caenorhabditis elegans, GI32563855, Length=183, Percent_Identity=49.1803278688525, Blast_Score=189, Evalue=3e-48,
Organism=Saccharomyces cerevisiae, GI6321693, Length=424, Percent_Identity=49.5283018867925, Blast_Score=389, Evalue=1e-109,
Organism=Saccharomyces cerevisiae, GI6323985, Length=424, Percent_Identity=48.5849056603774, Blast_Score=384, Evalue=1e-107,
Organism=Saccharomyces cerevisiae, GI6324974, Length=424, Percent_Identity=48.3490566037736, Blast_Score=382, Evalue=1e-107,
Organism=Saccharomyces cerevisiae, GI6324969, Length=424, Percent_Identity=48.3490566037736, Blast_Score=382, Evalue=1e-107,
Organism=Saccharomyces cerevisiae, GI6321968, Length=432, Percent_Identity=48.6111111111111, Blast_Score=370, Evalue=1e-103,
Organism=Drosophila melanogaster, GI24580918, Length=431, Percent_Identity=53.1322505800464, Blast_Score=421, Evalue=1e-118,
Organism=Drosophila melanogaster, GI24580916, Length=431, Percent_Identity=53.1322505800464, Blast_Score=421, Evalue=1e-118,
Organism=Drosophila melanogaster, GI24580920, Length=431, Percent_Identity=53.1322505800464, Blast_Score=421, Evalue=1e-118,
Organism=Drosophila melanogaster, GI24580914, Length=431, Percent_Identity=53.1322505800464, Blast_Score=421, Evalue=1e-118,
Organism=Drosophila melanogaster, GI281360527, Length=426, Percent_Identity=53.5211267605634, Blast_Score=419, Evalue=1e-117,
Organism=Drosophila melanogaster, GI17137654, Length=426, Percent_Identity=53.5211267605634, Blast_Score=419, Evalue=1e-117,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000941
- InterPro:   IPR020810
- InterPro:   IPR020809
- InterPro:   IPR020811 [H]

Pfam domain/function: PF00113 Enolase_C; PF03952 Enolase_N [H]

EC number: =4.2.1.11 [H]

Molecular weight: Translated: 47179; Mature: 47179

Theoretical pI: Translated: 4.57; Mature: 4.57

Prosite motif: PS00164 ENOLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYAEETNNVTAIKDIRAREILDSRGNPTIEADVILADGTIGRAAAPSGASTGSREALELR
CCCCCCCCEEHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCEEEEC
DGDQSRYMGKGVKKAVANVNSQIRSALMDKDVTAQQAIDDAMIALDGTENKDNLGANAIL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCHHEEEECCCCCCCCCCCHHHH
AVSLAVAKAAAKSQSLPLHQYIADLRNQTSLTMPVPMMNIINGGEHADNTVDIQEFMIEP
HHHHHHHHHHHHCCCCCHHHHHHHHHCCCCEECCCCHHHHHCCCCCCCCCEEHHHHHHCC
VGFSSFSEALRAGTEIFHSLKSVLKSQGLNTAVGDEGGFAPNLRSNEEAITVIMQAIEQV
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
GYTAGKDIHLALDCAATEFYKDGKYVLAGEGNKSFDSQGFSDYLVGLARQYPIISIEDGL
CCCCCCCEEEEEEHHHHHHHCCCCEEEECCCCCCCCCCCHHHHHHHHHHCCCEEEECCCC
DESDWDGWKYLTEQIGDKVQLVGDDLFVTNPAILQEGIDKKIANAILIKFNQIGTLSETL
CCCCCCHHHHHHHHHCCEEEEECCCEEECCHHHHHHHHHHHHHHHHEEEECCCCCHHHHH
DAIYLAKKNGYATVISHRSGETEDSTIADLAVGTAAGQIKTGSLCRSDRVAKYNQLLRIE
HHEEEEECCCEEEEEECCCCCCCCCHHHHHHHHCCCCCCCCCCCHHCCHHHHHHHHHHHH
QQVRASYRGREEFIGLRG
HHHHHHHCCHHHHCCCCC
>Mature Secondary Structure
MYAEETNNVTAIKDIRAREILDSRGNPTIEADVILADGTIGRAAAPSGASTGSREALELR
CCCCCCCCEEHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCEEEEC
DGDQSRYMGKGVKKAVANVNSQIRSALMDKDVTAQQAIDDAMIALDGTENKDNLGANAIL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCHHEEEECCCCCCCCCCCHHHH
AVSLAVAKAAAKSQSLPLHQYIADLRNQTSLTMPVPMMNIINGGEHADNTVDIQEFMIEP
HHHHHHHHHHHHCCCCCHHHHHHHHHCCCCEECCCCHHHHHCCCCCCCCCEEHHHHHHCC
VGFSSFSEALRAGTEIFHSLKSVLKSQGLNTAVGDEGGFAPNLRSNEEAITVIMQAIEQV
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
GYTAGKDIHLALDCAATEFYKDGKYVLAGEGNKSFDSQGFSDYLVGLARQYPIISIEDGL
CCCCCCCEEEEEEHHHHHHHCCCCEEEECCCCCCCCCCCHHHHHHHHHHCCCEEEECCCC
DESDWDGWKYLTEQIGDKVQLVGDDLFVTNPAILQEGIDKKIANAILIKFNQIGTLSETL
CCCCCCHHHHHHHHHCCEEEEECCCEEECCHHHHHHHHHHHHHHHHEEEECCCCCHHHHH
DAIYLAKKNGYATVISHRSGETEDSTIADLAVGTAAGQIKTGSLCRSDRVAKYNQLLRIE
HHEEEEECCCEEEEEECCCCCCCCCHHHHHHHHCCCCCCCCCCCHHCCHHHHHHHHHHHH
QQVRASYRGREEFIGLRG
HHHHHHHCCHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA