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The map label for this gene is otsB [H]

Identifier: 93006627

GI number: 93006627

Start: 2226618

End: 2227499

Strand: Reverse

Name: otsB [H]

Synonym: Pcryo_1803

Alternate gene names: 93006627

Gene position: 2227499-2226618 (Counterclockwise)

Preceding gene: 93006628

Following gene: 93006626

Centisome position: 72.8

GC content: 38.66

Gene sequence:

>882_bases
GTGACTTCAGGAAGAAATCCCAGTATAAATAGGCGACCTATCTATATTCTGCCAAAAAACTTTGCTGAATATTTATGCCC
TCAGCAAAATTATTATTTGTTTCTAGATATAGATGGTACGTTAGCGGATTTCACACTGAATCCAAAAGACAGCGTTATCC
CAACATCAACCTTAACTCTTCTACAAAAAATACAAAGCCATGGTGTAAAACTTGCTGTCGTAACAGGACGCAGTTTGGCT
GAAGCAAGGCAAATGCTGTCTCCTTTAGTGTTACCGATTGCCGCAACGCATGGACTAGAAATAGCGTTTGATGATATCAG
TGATCATAATGAGACACATGTTGTATCTGTTGATATCATGGAACTTGCTGTAATAAGAAAGTCTATTATCCAATCTTGCA
TTCCCTTTGATGACTTCACCGTAGAAGATAAACCCTATTCTGTCGCCCTCCATTTCCGAAAGAACCCTGTTTTGGCTGAT
GCGGCTTATACCATCATGATAGAAACCTTAAAAACTTATGCTAACTGGACATTGAAATCTGGTAAATATGTTTGGGAGGT
AGTGCCAAAAGGAGTGGATAAAGGTAGCGCAATTCTGACTTTATTAAAAAGAATGCAGAGTAGTAATAATATGTTCCCTA
TTTTTATCGGTGATGATATTACGGATGAAGTAGGGTTTGTGGCTGTACAAGGTGAAAATCAAATAATAGCCAGTCAACCA
CAGCCATTAAAAGGGATGGGGATTAAGGTTGGTAGTGAGGCTACGCATGCCAATTACTATGTACGTAATACTCATGAGGT
CACCATATTACTGAATAGCTTTTTAAGCTTTTGCCAAGAGCATTCAATGCTAGCAAAAGAAACAGTGAGGCACGTGATAT
GA

Upstream 100 bases:

>100_bases
AGATCTATATTAAAAACGACAGTAACTTTTAGCAGCTAAATTATTTCAAACTTAGGTGGTGTTAGAGCGAAAAATGGTCT
CATACTAAAAGGGAATAAAC

Downstream 100 bases:

>100_bases
GCCGCTTAATCGTCTTGTCTAATCGGGTTAAAATGCCTGATAAAAATCCAATGGCAGGCGGACTGGCGATAGCACTGCAA
GACGTGTTAATGGAAAAATC

Product: HAD family hydrolase

Products: NA

Alternate protein names: TPP; Trehalose 6-phosphate phosphatase; Trehalose-phosphatase [H]

Number of amino acids: Translated: 293; Mature: 292

Protein sequence:

>293_residues
MTSGRNPSINRRPIYILPKNFAEYLCPQQNYYLFLDIDGTLADFTLNPKDSVIPTSTLTLLQKIQSHGVKLAVVTGRSLA
EARQMLSPLVLPIAATHGLEIAFDDISDHNETHVVSVDIMELAVIRKSIIQSCIPFDDFTVEDKPYSVALHFRKNPVLAD
AAYTIMIETLKTYANWTLKSGKYVWEVVPKGVDKGSAILTLLKRMQSSNNMFPIFIGDDITDEVGFVAVQGENQIIASQP
QPLKGMGIKVGSEATHANYYVRNTHEVTILLNSFLSFCQEHSMLAKETVRHVI

Sequences:

>Translated_293_residues
MTSGRNPSINRRPIYILPKNFAEYLCPQQNYYLFLDIDGTLADFTLNPKDSVIPTSTLTLLQKIQSHGVKLAVVTGRSLA
EARQMLSPLVLPIAATHGLEIAFDDISDHNETHVVSVDIMELAVIRKSIIQSCIPFDDFTVEDKPYSVALHFRKNPVLAD
AAYTIMIETLKTYANWTLKSGKYVWEVVPKGVDKGSAILTLLKRMQSSNNMFPIFIGDDITDEVGFVAVQGENQIIASQP
QPLKGMGIKVGSEATHANYYVRNTHEVTILLNSFLSFCQEHSMLAKETVRHVI
>Mature_292_residues
TSGRNPSINRRPIYILPKNFAEYLCPQQNYYLFLDIDGTLADFTLNPKDSVIPTSTLTLLQKIQSHGVKLAVVTGRSLAE
ARQMLSPLVLPIAATHGLEIAFDDISDHNETHVVSVDIMELAVIRKSIIQSCIPFDDFTVEDKPYSVALHFRKNPVLADA
AYTIMIETLKTYANWTLKSGKYVWEVVPKGVDKGSAILTLLKRMQSSNNMFPIFIGDDITDEVGFVAVQGENQIIASQPQ
PLKGMGIKVGSEATHANYYVRNTHEVTILLNSFLSFCQEHSMLAKETVRHVI

Specific function: Removes the phosphate from trehalose 6-phosphate to produce free trehalose [H]

COG id: COG1877

COG function: function code G; Trehalose-6-phosphatase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trehalose phosphatase family [H]

Homologues:

Organism=Escherichia coli, GI1788207, Length=256, Percent_Identity=30.46875, Blast_Score=110, Evalue=1e-25,
Organism=Drosophila melanogaster, GI221473368, Length=217, Percent_Identity=27.6497695852535, Blast_Score=79, Evalue=3e-15,
Organism=Drosophila melanogaster, GI20129307, Length=217, Percent_Identity=27.6497695852535, Blast_Score=79, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24582490, Length=217, Percent_Identity=27.6497695852535, Blast_Score=79, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023214
- InterPro:   IPR006379
- InterPro:   IPR003337 [H]

Pfam domain/function: PF02358 Trehalose_PPase [H]

EC number: =3.1.3.12 [H]

Molecular weight: Translated: 32683; Mature: 32552

Theoretical pI: Translated: 6.60; Mature: 6.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSGRNPSINRRPIYILPKNFAEYLCPQQNYYLFLDIDGTLADFTLNPKDSVIPTSTLTL
CCCCCCCCCCCCEEEEECCCHHHHHCCCCCCEEEEEECCCEEEEEECCCCCCCCHHHHHH
LQKIQSHGVKLAVVTGRSLAEARQMLSPLVLPIAATHGLEIAFDDISDHNETHVVSVDIM
HHHHHHCCEEEEEEECCHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCEEEEEEHHH
ELAVIRKSIIQSCIPFDDFTVEDKPYSVALHFRKNPVLADAAYTIMIETLKTYANWTLKS
HHHHHHHHHHHHHCCCCCCEECCCCEEEEEEEECCCEEHHHHHHHHHHHHHHHHCEEECC
GKYVWEVVPKGVDKGSAILTLLKRMQSSNNMFPIFIGDDITDEVGFVAVQGENQIIASQP
CCEEEEEECCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCEEEEECCCEEEECCC
QPLKGMGIKVGSEATHANYYVRNTHEVTILLNSFLSFCQEHSMLAKETVRHVI
CCCCCCCEEECCCCCCCEEEEECCCEEEEHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TSGRNPSINRRPIYILPKNFAEYLCPQQNYYLFLDIDGTLADFTLNPKDSVIPTSTLTL
CCCCCCCCCCCEEEEECCCHHHHHCCCCCCEEEEEECCCEEEEEECCCCCCCCHHHHHH
LQKIQSHGVKLAVVTGRSLAEARQMLSPLVLPIAATHGLEIAFDDISDHNETHVVSVDIM
HHHHHHCCEEEEEEECCHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCEEEEEEHHH
ELAVIRKSIIQSCIPFDDFTVEDKPYSVALHFRKNPVLADAAYTIMIETLKTYANWTLKS
HHHHHHHHHHHHHCCCCCCEECCCCEEEEEEEECCCEEHHHHHHHHHHHHHHHHCEEECC
GKYVWEVVPKGVDKGSAILTLLKRMQSSNNMFPIFIGDDITDEVGFVAVQGENQIIASQP
CCEEEEEECCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCEEEEECCCEEEECCC
QPLKGMGIKVGSEATHANYYVRNTHEVTILLNSFLSFCQEHSMLAKETVRHVI
CCCCCCCEEECCCCCCCEEEEECCCEEEEHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11677609 [H]