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The map label for this gene is leuC [H]

Identifier: 93006398

GI number: 93006398

Start: 1930087

End: 1931505

Strand: Reverse

Name: leuC [H]

Synonym: Pcryo_1574

Alternate gene names: 93006398

Gene position: 1931505-1930087 (Counterclockwise)

Preceding gene: 93006401

Following gene: 93006397

Centisome position: 63.12

GC content: 48.56

Gene sequence:

>1419_bases
ATGGCCGGTCAAACGTTATATGACAAACTTTGGAATGCTCACGAAGTCACCAAGCGTGATGATGGTTCGAGCCTGATTTA
TATCGACCGCCATTTGCTACATGAAGTGACAAGTCCGCAAGCGTTTGAGGGTTTGGAATTGGCTAATAGAGCGCCGTGGC
GTCTGTCGGCTAATATTGCAAGCCCTGACCATAACGTACCGACAGTCACCAAAGAGCGCTCAGAAGGTGTGGCAGGTATC
AAGGATAAGGTCTCGCGCTTACAAGTACTGACGTTAGATGATAACTGTGCCAAATTTGATATCGCTGAGTTTACGATTAA
TGATGCTCGTCAAGGGATCTTGCATGTCGTCGGTCCTGAGCAAGGCTTGGTATTGCCTGGTATGACTGTCGTTTGCGGTG
ATTCACATACCGCCACTCATGGCGCATTGGGCTGTTTAGCCCACGGTATTGGTACTTCTGAAGTTGAGCACGTATTGGCA
ACCCAGTGCTTGATTCAAAAGAAATCAAAGAATATGCAAATCCGCGTCACCGGTGAGCTTGGCGCTGGCGTAACCTCAAA
AGATGTGGTGCTTGCTATCATTGCAAAAATCGGTACTGCTGGCGGAACAGGGCATGCGATTGAATTTGCTGGTCAAGTAT
TTGAAGACATGAGCATGGAAGGTCGTATGACCGTCTGTAATATGGCGATTGAAGCGGGCGCACGCGTGGGTATGGTTGCA
GTCGATGATACGACGATTGACTATGTTAAAGGTCGTCCATATGCGCCGAACGAGTCACAATGGCAGCAAGCAGAAGCTTA
CTGGCGTACTTTTTACTCAGATGATGACGCTGTGTTTGATAGCGTGATTGAGATTGATGGTAGCCAAATTGCACCGCAAG
TATCTTGGGGTACCTCGCCTGAGATGGTGGTTGATATCACACAGTCAGTGCCAACGCCTGATCAAGCGATTGATGAAGCA
CAAGAAGAAGGTTGGTTGCGTGCTTATACCTATATGGGTTTGGAGGCGGGTCAAAAAATTACCGATATTCAGCTGGACCG
TATCTTTATTGGCTCTTGTACCAACTCGCGTATTGAAGATTTGCGTGATGCCGCCGCCGTGATCAAAGGTCGTAAAGTGG
CTGATACTATTAAAGAAGCCATCGTCGTTGCTGGTTCTGGTCAAGTGAAGTTGCAGGCAGAAGCAGAAGGTCTAGATGCT
TTATTTACGGACGCAGGCTTTGAATGGCGTGAGCCGGGCTGCTCGATGTGCCTTGCAATGAATGCTGATAAGCTTGAGCC
ACAAGAGCATTGTGCTTCGACATCCAATCGCAACTTTGAAGGTCGACAAGGCAATGGTGGTCGCACTCATCTGGTCAGCC
CAGCAATGGCAGCAGCAGCAGCATTGGCAGGTCACTTTGTGGATGTGCGAACTTTTTAG

Upstream 100 bases:

>100_bases
CAATTATCGCAACCATTAGTAAGTAACAGATAAAAACCTAGCACACTATGGGTTTAATAAATGAAAAATATAGTCATTAG
CCACTAAAAAGGATAGCAAT

Downstream 100 bases:

>100_bases
ATATTACGCGTCATTTAACAATAAGCACAATATTGATAGGAAATACTATGCAAGCTTATAACACCCAAACAGGCATCGTT
TGCCCACTTGATCGTGCAAA

Product: isopropylmalate isomerase large subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase [H]

Number of amino acids: Translated: 472; Mature: 471

Protein sequence:

>472_residues
MAGQTLYDKLWNAHEVTKRDDGSSLIYIDRHLLHEVTSPQAFEGLELANRAPWRLSANIASPDHNVPTVTKERSEGVAGI
KDKVSRLQVLTLDDNCAKFDIAEFTINDARQGILHVVGPEQGLVLPGMTVVCGDSHTATHGALGCLAHGIGTSEVEHVLA
TQCLIQKKSKNMQIRVTGELGAGVTSKDVVLAIIAKIGTAGGTGHAIEFAGQVFEDMSMEGRMTVCNMAIEAGARVGMVA
VDDTTIDYVKGRPYAPNESQWQQAEAYWRTFYSDDDAVFDSVIEIDGSQIAPQVSWGTSPEMVVDITQSVPTPDQAIDEA
QEEGWLRAYTYMGLEAGQKITDIQLDRIFIGSCTNSRIEDLRDAAAVIKGRKVADTIKEAIVVAGSGQVKLQAEAEGLDA
LFTDAGFEWREPGCSMCLAMNADKLEPQEHCASTSNRNFEGRQGNGGRTHLVSPAMAAAAALAGHFVDVRTF

Sequences:

>Translated_472_residues
MAGQTLYDKLWNAHEVTKRDDGSSLIYIDRHLLHEVTSPQAFEGLELANRAPWRLSANIASPDHNVPTVTKERSEGVAGI
KDKVSRLQVLTLDDNCAKFDIAEFTINDARQGILHVVGPEQGLVLPGMTVVCGDSHTATHGALGCLAHGIGTSEVEHVLA
TQCLIQKKSKNMQIRVTGELGAGVTSKDVVLAIIAKIGTAGGTGHAIEFAGQVFEDMSMEGRMTVCNMAIEAGARVGMVA
VDDTTIDYVKGRPYAPNESQWQQAEAYWRTFYSDDDAVFDSVIEIDGSQIAPQVSWGTSPEMVVDITQSVPTPDQAIDEA
QEEGWLRAYTYMGLEAGQKITDIQLDRIFIGSCTNSRIEDLRDAAAVIKGRKVADTIKEAIVVAGSGQVKLQAEAEGLDA
LFTDAGFEWREPGCSMCLAMNADKLEPQEHCASTSNRNFEGRQGNGGRTHLVSPAMAAAAALAGHFVDVRTF
>Mature_471_residues
AGQTLYDKLWNAHEVTKRDDGSSLIYIDRHLLHEVTSPQAFEGLELANRAPWRLSANIASPDHNVPTVTKERSEGVAGIK
DKVSRLQVLTLDDNCAKFDIAEFTINDARQGILHVVGPEQGLVLPGMTVVCGDSHTATHGALGCLAHGIGTSEVEHVLAT
QCLIQKKSKNMQIRVTGELGAGVTSKDVVLAIIAKIGTAGGTGHAIEFAGQVFEDMSMEGRMTVCNMAIEAGARVGMVAV
DDTTIDYVKGRPYAPNESQWQQAEAYWRTFYSDDDAVFDSVIEIDGSQIAPQVSWGTSPEMVVDITQSVPTPDQAIDEAQ
EEGWLRAYTYMGLEAGQKITDIQLDRIFIGSCTNSRIEDLRDAAAVIKGRKVADTIKEAIVVAGSGQVKLQAEAEGLDAL
FTDAGFEWREPGCSMCLAMNADKLEPQEHCASTSNRNFEGRQGNGGRTHLVSPAMAAAAALAGHFVDVRTF

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate [H]

COG id: COG0065

COG function: function code E; 3-isopropylmalate dehydratase large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aconitase/IPM isomerase family. LeuC type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI4501867, Length=363, Percent_Identity=27.8236914600551, Blast_Score=103, Evalue=3e-22,
Organism=Homo sapiens, GI41352693, Length=384, Percent_Identity=27.6041666666667, Blast_Score=100, Evalue=3e-21,
Organism=Homo sapiens, GI8659555, Length=436, Percent_Identity=25.4587155963303, Blast_Score=95, Evalue=1e-19,
Organism=Escherichia coli, GI1786259, Length=469, Percent_Identity=56.7164179104478, Blast_Score=547, Evalue=1e-157,
Organism=Escherichia coli, GI1787531, Length=361, Percent_Identity=26.5927977839335, Blast_Score=92, Evalue=6e-20,
Organism=Caenorhabditis elegans, GI25149337, Length=367, Percent_Identity=29.700272479564, Blast_Score=120, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI32564738, Length=367, Percent_Identity=29.700272479564, Blast_Score=119, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI25149342, Length=312, Percent_Identity=29.1666666666667, Blast_Score=110, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI17568399, Length=383, Percent_Identity=26.1096605744125, Blast_Score=99, Evalue=6e-21,
Organism=Saccharomyces cerevisiae, GI6321429, Length=470, Percent_Identity=59.5744680851064, Blast_Score=568, Evalue=1e-163,
Organism=Saccharomyces cerevisiae, GI6320440, Length=418, Percent_Identity=28.2296650717703, Blast_Score=128, Evalue=2e-30,
Organism=Saccharomyces cerevisiae, GI6322261, Length=364, Percent_Identity=27.7472527472527, Blast_Score=122, Evalue=2e-28,
Organism=Saccharomyces cerevisiae, GI6323335, Length=364, Percent_Identity=27.7472527472527, Blast_Score=121, Evalue=2e-28,
Organism=Drosophila melanogaster, GI281365315, Length=385, Percent_Identity=27.7922077922078, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI17864292, Length=385, Percent_Identity=27.7922077922078, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI161076999, Length=385, Percent_Identity=27.7922077922078, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI28571643, Length=385, Percent_Identity=25.4545454545455, Blast_Score=95, Evalue=1e-19,
Organism=Drosophila melanogaster, GI24645686, Length=383, Percent_Identity=28.7206266318538, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI17137564, Length=382, Percent_Identity=26.9633507853403, Blast_Score=86, Evalue=5e-17,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004430
- InterPro:   IPR015931
- InterPro:   IPR015937
- InterPro:   IPR001030
- InterPro:   IPR015932
- InterPro:   IPR018136
- InterPro:   IPR015936 [H]

Pfam domain/function: PF00330 Aconitase [H]

EC number: =4.2.1.33 [H]

Molecular weight: Translated: 50878; Mature: 50747

Theoretical pI: Translated: 4.65; Mature: 4.65

Prosite motif: PS00450 ACONITASE_1 ; PS01244 ACONITASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAGQTLYDKLWNAHEVTKRDDGSSLIYIDRHLLHEVTSPQAFEGLELANRAPWRLSANIA
CCCCHHHHHHCCHHHCCCCCCCCEEEEEEHHHHHHCCCCCHHCCHHHHCCCCCEEEEECC
SPDHNVPTVTKERSEGVAGIKDKVSRLQVLTLDDNCAKFDIAEFTINDARQGILHVVGPE
CCCCCCCCCCCCCCCCCCCHHHHHCEEEEEEECCCCCEEEEEEEECCCCCCCEEEEECCC
QGLVLPGMTVVCGDSHTATHGALGCLAHGIGTSEVEHVLATQCLIQKKSKNMQIRVTGEL
CCEECCCEEEEECCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCEEEEEEECC
GAGVTSKDVVLAIIAKIGTAGGTGHAIEFAGQVFEDMSMEGRMTVCNMAIEAGARVGMVA
CCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCEEHHHHHCCCEEEEEE
VDDTTIDYVKGRPYAPNESQWQQAEAYWRTFYSDDDAVFDSVIEIDGSQIAPQVSWGTSP
ECCCEEEHCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCCCCCCCC
EMVVDITQSVPTPDQAIDEAQEEGWLRAYTYMGLEAGQKITDIQLDRIFIGSCTNSRIED
CEEEEECCCCCCCHHHHHHHHHCCCEEEEEEECHHCCCEEEEEEEEEEEEECCCCHHHHH
LRDAAAVIKGRKVADTIKEAIVVAGSGQVKLQAEAEGLDALFTDAGFEWREPGCSMCLAM
HHHHHHHHCCCHHHHHHHHHEEEECCCCEEEEECCCCCHHHHHCCCCCCCCCCCCEEEEC
NADKLEPQEHCASTSNRNFEGRQGNGGRTHLVSPAMAAAAALAGHFVDVRTF
CCCCCCHHHHHCCCCCCCCCCCCCCCCCEEECCHHHHHHHHHHHHEEEEECC
>Mature Secondary Structure 
AGQTLYDKLWNAHEVTKRDDGSSLIYIDRHLLHEVTSPQAFEGLELANRAPWRLSANIA
CCCHHHHHHCCHHHCCCCCCCCEEEEEEHHHHHHCCCCCHHCCHHHHCCCCCEEEEECC
SPDHNVPTVTKERSEGVAGIKDKVSRLQVLTLDDNCAKFDIAEFTINDARQGILHVVGPE
CCCCCCCCCCCCCCCCCCCHHHHHCEEEEEEECCCCCEEEEEEEECCCCCCCEEEEECCC
QGLVLPGMTVVCGDSHTATHGALGCLAHGIGTSEVEHVLATQCLIQKKSKNMQIRVTGEL
CCEECCCEEEEECCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCEEEEEEECC
GAGVTSKDVVLAIIAKIGTAGGTGHAIEFAGQVFEDMSMEGRMTVCNMAIEAGARVGMVA
CCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCEEHHHHHCCCEEEEEE
VDDTTIDYVKGRPYAPNESQWQQAEAYWRTFYSDDDAVFDSVIEIDGSQIAPQVSWGTSP
ECCCEEEHCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCCCCCCCC
EMVVDITQSVPTPDQAIDEAQEEGWLRAYTYMGLEAGQKITDIQLDRIFIGSCTNSRIED
CEEEEECCCCCCCHHHHHHHHHCCCEEEEEEECHHCCCEEEEEEEEEEEEECCCCHHHHH
LRDAAAVIKGRKVADTIKEAIVVAGSGQVKLQAEAEGLDALFTDAGFEWREPGCSMCLAM
HHHHHHHHCCCHHHHHHHHHEEEECCCCEEEEECCCCCHHHHHCCCCCCCCCCCCEEEEC
NADKLEPQEHCASTSNRNFEGRQGNGGRTHLVSPAMAAAAALAGHFVDVRTF
CCCCCCHHHHHCCCCCCCCCCCCCCCCCEEECCHHHHHHHHHHHHEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA