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The map label for this gene is leuD [H]

Identifier: 93006397

GI number: 93006397

Start: 1929389

End: 1930039

Strand: Reverse

Name: leuD [H]

Synonym: Pcryo_1573

Alternate gene names: 93006397

Gene position: 1930039-1929389 (Counterclockwise)

Preceding gene: 93006398

Following gene: 93006396

Centisome position: 63.08

GC content: 43.01

Gene sequence:

>651_bases
ATGCAAGCTTATAACACCCAAACAGGCATCGTTTGCCCACTTGATCGTGCAAACGTCGATACCGACCAGATTATCGCAAA
ACAGTTTTTGAAGTCTATTAAGCGTACCGGTTTTGGCGTCAATTTATTTGATGATTGGCGTTATCTGGATGAAGGGTATC
CGGGACAAGACAATAGCACACGTGTTATCAATCCTGACTTTATTTTAAATAAACCACGATATCAAGGCGCTAGTATCTTA
CTGGCGCGCCGTAACTTTGGCTGTGGCTCTAGCCGTGAACACGCGCCTTGGGCGCTTTCAGAGTATGGCTTTCGTACAGT
CATTGCACCAAGCTTTGCCGATATTTTTTATAACAACTGTTTTAAAAATGGCATGCTACCGATTGTATTGGATGAAGAAA
TCGTTGATAGCCTAATGAAAGCCACGTTTGCCAACGAAGGCTATGAATTGACTGCAGATCTTGAGCGTCAGGTGGTCATC
ACCCCAACTGGCGAAGAGTATGCGTTTGAAGTCGATGACTTCCGTAAGCATTGCCTATTAAATGGTCTTGATGATATTGG
ACTGACGTTGCAGCAAAGCGATGCTATTAAAGAATACGAGCAAAAAATGATGCAAAAAACGCCTTGGATATTTAATGAAG
TCAGAGCCTAG

Upstream 100 bases:

>100_bases
TGGCAGCAGCAGCAGCATTGGCAGGTCACTTTGTGGATGTGCGAACTTTTTAGATATTACGCGTCATTTAACAATAAGCA
CAATATTGATAGGAAATACT

Downstream 100 bases:

>100_bases
TGTTTAAGCATGAAATAAAACCGCTAATGGCAAACGATTAATAACGAATATGAATCGCCATAAAAACGAGCTTAGCGCTA
ATACAGCGTTGAGTTATGGC

Product: isopropylmalate isomerase small subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase [H]

Number of amino acids: Translated: 216; Mature: 216

Protein sequence:

>216_residues
MQAYNTQTGIVCPLDRANVDTDQIIAKQFLKSIKRTGFGVNLFDDWRYLDEGYPGQDNSTRVINPDFILNKPRYQGASIL
LARRNFGCGSSREHAPWALSEYGFRTVIAPSFADIFYNNCFKNGMLPIVLDEEIVDSLMKATFANEGYELTADLERQVVI
TPTGEEYAFEVDDFRKHCLLNGLDDIGLTLQQSDAIKEYEQKMMQKTPWIFNEVRA

Sequences:

>Translated_216_residues
MQAYNTQTGIVCPLDRANVDTDQIIAKQFLKSIKRTGFGVNLFDDWRYLDEGYPGQDNSTRVINPDFILNKPRYQGASIL
LARRNFGCGSSREHAPWALSEYGFRTVIAPSFADIFYNNCFKNGMLPIVLDEEIVDSLMKATFANEGYELTADLERQVVI
TPTGEEYAFEVDDFRKHCLLNGLDDIGLTLQQSDAIKEYEQKMMQKTPWIFNEVRA
>Mature_216_residues
MQAYNTQTGIVCPLDRANVDTDQIIAKQFLKSIKRTGFGVNLFDDWRYLDEGYPGQDNSTRVINPDFILNKPRYQGASIL
LARRNFGCGSSREHAPWALSEYGFRTVIAPSFADIFYNNCFKNGMLPIVLDEEIVDSLMKATFANEGYELTADLERQVVI
TPTGEEYAFEVDDFRKHCLLNGLDDIGLTLQQSDAIKEYEQKMMQKTPWIFNEVRA

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate [H]

COG id: COG0066

COG function: function code E; 3-isopropylmalate dehydratase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leuD family. LeuD type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786258, Length=197, Percent_Identity=61.4213197969543, Blast_Score=241, Evalue=3e-65,
Organism=Saccharomyces cerevisiae, GI6321429, Length=210, Percent_Identity=50.9523809523809, Blast_Score=202, Evalue=4e-53,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004431
- InterPro:   IPR012305
- InterPro:   IPR015937
- InterPro:   IPR015928
- InterPro:   IPR000573 [H]

Pfam domain/function: PF00694 Aconitase_C [H]

EC number: =4.2.1.33 [H]

Molecular weight: Translated: 24675; Mature: 24675

Theoretical pI: Translated: 4.54; Mature: 4.54

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQAYNTQTGIVCPLDRANVDTDQIIAKQFLKSIKRTGFGVNLFDDWRYLDEGYPGQDNST
CCCCCCCCCEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCCCCC
RVINPDFILNKPRYQGASILLARRNFGCGSSREHAPWALSEYGFRTVIAPSFADIFYNNC
EEECCCCEECCCCCCCCEEEEEECCCCCCCCCCCCCEEHHHCCCEEEECHHHHHHHHHHH
FKNGMLPIVLDEEIVDSLMKATFANEGYELTADLERQVVITPTGEEYAFEVDDFRKHCLL
HHCCCEEEEECHHHHHHHHHHHHCCCCEEEEECCCCEEEEEECCCCEEEEHHHHHHHHHH
NGLDDIGLTLQQSDAIKEYEQKMMQKTPWIFNEVRA
CCCHHHCCEECCHHHHHHHHHHHHHHCCCHHHHHCC
>Mature Secondary Structure
MQAYNTQTGIVCPLDRANVDTDQIIAKQFLKSIKRTGFGVNLFDDWRYLDEGYPGQDNST
CCCCCCCCCEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCCCCC
RVINPDFILNKPRYQGASILLARRNFGCGSSREHAPWALSEYGFRTVIAPSFADIFYNNC
EEECCCCEECCCCCCCCEEEEEECCCCCCCCCCCCCEEHHHCCCEEEECHHHHHHHHHHH
FKNGMLPIVLDEEIVDSLMKATFANEGYELTADLERQVVITPTGEEYAFEVDDFRKHCLL
HHCCCEEEEECHHHHHHHHHHHHCCCCEEEEECCCCEEEEEECCCCEEEEHHHHHHHHHH
NGLDDIGLTLQQSDAIKEYEQKMMQKTPWIFNEVRA
CCCHHHCCEECCHHHHHHHHHHHHHHCCCHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA