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The map label for this gene is epsE [H]

Identifier: 93006359

GI number: 93006359

Start: 1883503

End: 1885290

Strand: Reverse

Name: epsE [H]

Synonym: Pcryo_1533

Alternate gene names: 93006359

Gene position: 1885290-1883503 (Counterclockwise)

Preceding gene: 93006360

Following gene: 93006358

Centisome position: 61.61

GC content: 45.86

Gene sequence:

>1788_bases
ATGCCTGCTCAACAGTATTCGATAGTAATTGATTTGCGTTGGTGCCTTGATGAGTTGCTGGCAGATCGAGTGATAGACCA
ACGGGGTTATAATCTCGTCATTACCAGCCGCCGCAGTAAGGGTCAGCATCCGCTATTGACTATAAGTGAGTTTGGTCTGC
CTAATAGTCATGCACTTGATAATAATACTGAAAATAAATTAACTTTGGCATGGCTCAATCAATGGTTGGCTGCCAAAGCA
GATATGTCGCTGGTCCGTATTGACCCGTTAAAAGTCGATGTACCAGCAGTGACGCAGCTCATGTCGTTTGAGTATGCACG
CTCGCAGCATATTTTGCCGATTGAAGTAACGCTTGATGAGGTGGTTATCGGTACCGATCAGCCATTTTATACCGATTGGC
ATGGCAATATTGAAAAGCTCATTAAGTCAAAAAGCTATCGTACAGTCTTTATTAATCCTGAACAAATCAATCGCTATCGG
CAAGAATTTTACCAAGTCACGCAAGCGATTGCAGGGGCGAACTCACTACATAAACGTGCCGCATCTGATATCACTAATGT
TGAAGCACTATTACAGCTAGGGGACAATACCAACCCCGATGCCAATGACCAACATATTGTCAAAGTCGTCGATTGGTTGT
TGCAATATGCCTTTGAGCAGCGCGCCAGTGATATCCATTTAGAGCCACGTCGTGAGACGGGCAAGGTGCGTTTTCGCATT
GATGGGGTGCTGCATAGTGTTTATGAGATGCCACTCGCTATTATAGTGGCGGTAACGGCACGTATTAAAATCTTGGGACG
GCTCAATGTTGCAGAAAAACGCAAACCACAAGACGGGCGACTAAAAACACGTACACCAAAAGGCTTAGAGACGGAGCTGC
GTTTATCCACGATGCCGACTGCCTTTGGTGAAAAGTTGGTTATGCGGGTATTTGACCCTGAGGTACTAGTACGCTCCTTT
GCTCAGCTTGGTTTGTCAGGTAAGCAGCTGGAGACTTGGCACGAGCTGACAGCGCATCCAAACGGTATTATTCTAGTGAC
AGGTCCAACAGGTTCGGGTAAAACGACTACCTTATATAGTACGCTGAAGCAGCTCGCCACTGAACAAGTAAACGTCTGTA
CCATTGAAGATCCGATTGAGATGATTGAGTCAGCATTTAATCAAATGCAGGTTAATCCAGGAGTTGATTTACATTTCGCT
GATGGTATTCGCTCTTTGATGCGTCAAGACCCTGATATTATTATGGTTGGTGAGATTCGTGATGCTGAAACGGCAAACAT
GGCGGTACAAGCATCTTTAACAGGGCATTTGGTGCTCTCAACCTTGCATACCAATGATGCGCCAAGTTCAATTACTCGAC
TGCACGATTTGGGTATTCAGCCTTTTTTGACGTCAGCAACGATATTGGGCATCATGGCACAACGTTTATTACGGACATTA
TGTCCACATTGTAAGCAAGCCGTGGATGTTACGGCAGATAGCGAGATTGCCATTCAATGGCAAGAATTAGTACAGCCGTG
GCGCGCGTCCGTGCCAGCGCAGATTTATAAGGCACAGGGTTGCGAGCATTGCCGCCATACTGGTTATCAAGGTCGTATTG
GTCTTTATGAGATTATGCCATTATCAAATGAGTTGAAAAAACTGGTCGCTGCTGATGCCAATCTGGATGTATTAAAGCAG
CAAGCATACCGTGAAGGCGTACAGCCATTACGCCTATCAGGTGCGAAGCGTATTAGTGAAGGGGTAACGACCATGGAAGA
GGTGATGCGCGTGGTACCTTTGCATTAG

Upstream 100 bases:

>100_bases
AGGTGAATTACAGATAACGGATATCGACACAATATAGTCTAATAGGTAAGATGAATATCAACATTGCGCAAATGCTTTAA
ACCATAAAAGAAGAAAAAAT

Downstream 100 bases:

>100_bases
TAGAGAGCCGCTATATTAAAAGATGCCATTGCATTAAAAGTAAGTTTGTTAAGCAGTGCTTGAAAAGTGCCCGCTTCAAA
ACAATAAATACGATACAAAT

Product: type II secretion system protein E

Products: NA

Alternate protein names: Cholera toxin secretion protein epsE; Type II traffic warden ATPase [H]

Number of amino acids: Translated: 595; Mature: 594

Protein sequence:

>595_residues
MPAQQYSIVIDLRWCLDELLADRVIDQRGYNLVITSRRSKGQHPLLTISEFGLPNSHALDNNTENKLTLAWLNQWLAAKA
DMSLVRIDPLKVDVPAVTQLMSFEYARSQHILPIEVTLDEVVIGTDQPFYTDWHGNIEKLIKSKSYRTVFINPEQINRYR
QEFYQVTQAIAGANSLHKRAASDITNVEALLQLGDNTNPDANDQHIVKVVDWLLQYAFEQRASDIHLEPRRETGKVRFRI
DGVLHSVYEMPLAIIVAVTARIKILGRLNVAEKRKPQDGRLKTRTPKGLETELRLSTMPTAFGEKLVMRVFDPEVLVRSF
AQLGLSGKQLETWHELTAHPNGIILVTGPTGSGKTTTLYSTLKQLATEQVNVCTIEDPIEMIESAFNQMQVNPGVDLHFA
DGIRSLMRQDPDIIMVGEIRDAETANMAVQASLTGHLVLSTLHTNDAPSSITRLHDLGIQPFLTSATILGIMAQRLLRTL
CPHCKQAVDVTADSEIAIQWQELVQPWRASVPAQIYKAQGCEHCRHTGYQGRIGLYEIMPLSNELKKLVAADANLDVLKQ
QAYREGVQPLRLSGAKRISEGVTTMEEVMRVVPLH

Sequences:

>Translated_595_residues
MPAQQYSIVIDLRWCLDELLADRVIDQRGYNLVITSRRSKGQHPLLTISEFGLPNSHALDNNTENKLTLAWLNQWLAAKA
DMSLVRIDPLKVDVPAVTQLMSFEYARSQHILPIEVTLDEVVIGTDQPFYTDWHGNIEKLIKSKSYRTVFINPEQINRYR
QEFYQVTQAIAGANSLHKRAASDITNVEALLQLGDNTNPDANDQHIVKVVDWLLQYAFEQRASDIHLEPRRETGKVRFRI
DGVLHSVYEMPLAIIVAVTARIKILGRLNVAEKRKPQDGRLKTRTPKGLETELRLSTMPTAFGEKLVMRVFDPEVLVRSF
AQLGLSGKQLETWHELTAHPNGIILVTGPTGSGKTTTLYSTLKQLATEQVNVCTIEDPIEMIESAFNQMQVNPGVDLHFA
DGIRSLMRQDPDIIMVGEIRDAETANMAVQASLTGHLVLSTLHTNDAPSSITRLHDLGIQPFLTSATILGIMAQRLLRTL
CPHCKQAVDVTADSEIAIQWQELVQPWRASVPAQIYKAQGCEHCRHTGYQGRIGLYEIMPLSNELKKLVAADANLDVLKQ
QAYREGVQPLRLSGAKRISEGVTTMEEVMRVVPLH
>Mature_594_residues
PAQQYSIVIDLRWCLDELLADRVIDQRGYNLVITSRRSKGQHPLLTISEFGLPNSHALDNNTENKLTLAWLNQWLAAKAD
MSLVRIDPLKVDVPAVTQLMSFEYARSQHILPIEVTLDEVVIGTDQPFYTDWHGNIEKLIKSKSYRTVFINPEQINRYRQ
EFYQVTQAIAGANSLHKRAASDITNVEALLQLGDNTNPDANDQHIVKVVDWLLQYAFEQRASDIHLEPRRETGKVRFRID
GVLHSVYEMPLAIIVAVTARIKILGRLNVAEKRKPQDGRLKTRTPKGLETELRLSTMPTAFGEKLVMRVFDPEVLVRSFA
QLGLSGKQLETWHELTAHPNGIILVTGPTGSGKTTTLYSTLKQLATEQVNVCTIEDPIEMIESAFNQMQVNPGVDLHFAD
GIRSLMRQDPDIIMVGEIRDAETANMAVQASLTGHLVLSTLHTNDAPSSITRLHDLGIQPFLTSATILGIMAQRLLRTLC
PHCKQAVDVTADSEIAIQWQELVQPWRASVPAQIYKAQGCEHCRHTGYQGRIGLYEIMPLSNELKKLVAADANLDVLKQQ
AYREGVQPLRLSGAKRISEGVTTMEEVMRVVPLH

Specific function: Required for secretion of cholera toxin through the outer membrane [H]

COG id: COG2804

COG function: function code NU; Type II secretory pathway, ATPase PulE/Tfp pilus assembly pathway, ATPase PilB

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI1789723, Length=386, Percent_Identity=42.4870466321244, Blast_Score=327, Evalue=9e-91,
Organism=Escherichia coli, GI1786296, Length=382, Percent_Identity=41.0994764397906, Blast_Score=309, Evalue=3e-85,
Organism=Escherichia coli, GI87082188, Length=126, Percent_Identity=44.4444444444444, Blast_Score=107, Evalue=3e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR013369
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 66783; Mature: 66651

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: PS00124 FBPASE ; PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPAQQYSIVIDLRWCLDELLADRVIDQRGYNLVITSRRSKGQHPLLTISEFGLPNSHALD
CCCCCEEEEEEHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCEEEEHHCCCCCCCCCC
NNTENKLTLAWLNQWLAAKADMSLVRIDPLKVDVPAVTQLMSFEYARSQHILPIEVTLDE
CCCCCEEEHHHHHHHHHHHCCCEEEEECCCEECHHHHHHHHHHHHHCCCCEEEEEEEECC
VVIGTDQPFYTDWHGNIEKLIKSKSYRTVFINPEQINRYRQEFYQVTQAIAGANSLHKRA
EEECCCCCEEECCCCCHHHHHCCCCCEEEEECHHHHHHHHHHHHHHHHHHHCHHHHHHHH
ASDITNVEALLQLGDNTNPDANDQHIVKVVDWLLQYAFEQRASDIHLEPRRETGKVRFRI
HHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEE
DGVLHSVYEMPLAIIVAVTARIKILGRLNVAEKRKPQDGRLKTRTPKGLETELRLSTMPT
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCEECCCCCCCCCEEEEECCCH
AFGEKLVMRVFDPEVLVRSFAQLGLSGKQLETWHELTAHPNGIILVTGPTGSGKTTTLYS
HHHHHHHHHHCCHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCEEEEECCCCCCCCHHHHH
TLKQLATEQVNVCTIEDPIEMIESAFNQMQVNPGVDLHFADGIRSLMRQDPDIIMVGEIR
HHHHHHHCCCCEEEEHHHHHHHHHHHHHEECCCCCCEEHHHHHHHHHHCCCCEEEEECCC
DAETANMAVQASLTGHLVLSTLHTNDAPSSITRLHDLGIQPFLTSATILGIMAQRLLRTL
CCCCCCEEEEEECCCEEEEEEEECCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
CPHCKQAVDVTADSEIAIQWQELVQPWRASVPAQIYKAQGCEHCRHTGYQGRIGLYEIMP
CHHHHHHHCCCCCCHHEEEHHHHHHHHHCCCCHHHHHHCCHHHHHHCCCCCCCCEEEECC
LSNELKKLVAADANLDVLKQQAYREGVQPLRLSGAKRISEGVTTMEEVMRVVPLH
CHHHHHHHHHCCCCHHHHHHHHHHCCCCCCHHCCHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
PAQQYSIVIDLRWCLDELLADRVIDQRGYNLVITSRRSKGQHPLLTISEFGLPNSHALD
CCCCEEEEEEHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCEEEEHHCCCCCCCCCC
NNTENKLTLAWLNQWLAAKADMSLVRIDPLKVDVPAVTQLMSFEYARSQHILPIEVTLDE
CCCCCEEEHHHHHHHHHHHCCCEEEEECCCEECHHHHHHHHHHHHHCCCCEEEEEEEECC
VVIGTDQPFYTDWHGNIEKLIKSKSYRTVFINPEQINRYRQEFYQVTQAIAGANSLHKRA
EEECCCCCEEECCCCCHHHHHCCCCCEEEEECHHHHHHHHHHHHHHHHHHHCHHHHHHHH
ASDITNVEALLQLGDNTNPDANDQHIVKVVDWLLQYAFEQRASDIHLEPRRETGKVRFRI
HHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEE
DGVLHSVYEMPLAIIVAVTARIKILGRLNVAEKRKPQDGRLKTRTPKGLETELRLSTMPT
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCEECCCCCCCCCEEEEECCCH
AFGEKLVMRVFDPEVLVRSFAQLGLSGKQLETWHELTAHPNGIILVTGPTGSGKTTTLYS
HHHHHHHHHHCCHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCEEEEECCCCCCCCHHHHH
TLKQLATEQVNVCTIEDPIEMIESAFNQMQVNPGVDLHFADGIRSLMRQDPDIIMVGEIR
HHHHHHHCCCCEEEEHHHHHHHHHHHHHEECCCCCCEEHHHHHHHHHHCCCCEEEEECCC
DAETANMAVQASLTGHLVLSTLHTNDAPSSITRLHDLGIQPFLTSATILGIMAQRLLRTL
CCCCCCEEEEEECCCEEEEEEEECCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
CPHCKQAVDVTADSEIAIQWQELVQPWRASVPAQIYKAQGCEHCRHTGYQGRIGLYEIMP
CHHHHHHHCCCCCCHHEEEHHHHHHHHHCCCCHHHHHHCCHHHHHHCCCCCCCCEEEECC
LSNELKKLVAADANLDVLKQQAYREGVQPLRLSGAKRISEGVTTMEEVMRVVPLH
CHHHHHHHHHCCCCHHHHHHHHHHCCCCCCHHCCHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8423007; 10952301 [H]