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The map label for this gene is alkK [H]

Identifier: 93006345

GI number: 93006345

Start: 1868431

End: 1870101

Strand: Reverse

Name: alkK [H]

Synonym: Pcryo_1519

Alternate gene names: 93006345

Gene position: 1870101-1868431 (Counterclockwise)

Preceding gene: 93006346

Following gene: 93006344

Centisome position: 61.12

GC content: 44.05

Gene sequence:

>1671_bases
ATGTCAAATATCTATCACAGTGCGCCATCCGCCTATGATTTTCCGCTATTGGTTAAGCAGTTATTAAACCGCGCCAAAAC
CGTATCACAAGATCAAGAAATCGTTTATGCAGATAAAAAACGTTTTACTTATAAAGACTTGTTTAACCGTATCAATCGTT
TGGCGAATGTTTTAGACGGCTTAAACCTTGATGCTGGTGATGTGGTTGCTGTGATGGATTGGGACAGTCATCGCTATCTT
GAATCCTACTTTGCCGTACCAATGTCAGAGTATATTTTGCAGACGGTCAATATTCGTCTATCACCTGACAAAATCCTTTA
CACCATTAATCATGCGAAACCAAAGGTGCTGCTACTCAACTCTGAATTTGCGCCGTTGGTCAAAGATTATCAGTTTGAAA
ACTCCAGCATTGAGCATATTATTTGGTTAGATGATAATGGTGTGACCTCTGAGGGCGTATTCGGGGGTAACCAGAACCGT
GTCAAAGGTGAATATGAGGCGCTACTTGATGCTGCTGATGACCATTTTGATTTCCCAGATTTTGATGAAAACACCATTGC
TACAACTTTTTATACCTCAGGTACGACAGGTGATCCGAAAGGCGTGTTCTTTAGCCATCGTCAGTTAGTACTGCATACCC
TGACGGAAGCAGCGTCGATTGGTATGTTGCCAAATAAACAAGGCGTCAGCTATGGTGATGTCTATATGCCAATGACCCCG
ATGTTCCATGTCCATGCGTGGGGCTTTCCGTTTACAGCTACTATGACAGGTCTAAAGCAAGTATATCCTGGTCGTTATGC
ACCCGATTTGTTGATGGATTTGATTATCAATGAAAAGGTCAGCATCACCCACTGTGTACCGACAATTTTGCAAATGGTAC
TCAAAGAAGCGCAAGACCGCGGTGCCAGCTTTAATGGTCTAAAAATGATTATTGGTGGTTCACGATTGACAGAAGGCTTG
GCAAAAGCGGCGATGGATCAGGGTATTGAAGTTTATACCGGTTATGGGATGTCGGAGACCGCACCTCTAATCAGTCTGAC
TGACTTTAGCCTCAATGAGCCGCAGCTGAGTGAAGAAGAGGATATTGCTCGCCGCTGTATGACGGGTAAGCCTGTACTTA
TGGTTGATGCTCAAGTATGGGGAGAGAACAATCAGCCAGTTGGTACAGGTCAGGATAATACTGGCGAGCTGGTATTACGC
GCACCGTGGTTGACCCAAAGCTATTTAAAAAATGATGACGCTGGCAAAGAGCTGTGGGAAAACGGCTACATGCATACTCA
AGACATCGCTTATATACGACCTGATGGCTATATCAAAATCACCGATCGCTTAAAAGATGTGATTAAGTCAGGTGGCGAGT
GGATATCTTCACTAGAGATTGAGACGATTCTATCGCTACATCCTTCGGTGGCGGATGTGTCTGTGATTGGGGTACGTGAT
AAGCAATGGGGCGAACGTCCATTAGCATTAGTAGTACTCAAACCAAATTGCCAAGATACCAAAGTTGATGACATCAAAGC
GCTGGCAGAGCAGGCAGTGGCCCGTGGTATCATTCCAAAATACGGTATACCTAGTCAGTTTAAGTTTGTAGATGAACTAC
CAAAGACCTCTGTTGGTAAACACGATAAAAAAGTGATGCGTGAGATGTATGCCAATCAGAGCGAGGTATAG

Upstream 100 bases:

>100_bases
GAGGTTATTGATAAAAACATCACCATGTAATCACGACTGATATCCAATCAATTGGTTTTTATCTGCACTCAAAAATACTA
TAAACAAGGACGTCAAACTT

Downstream 100 bases:

>100_bases
TTTTTTTGAACTAACTCAACTATTAAGCAGATAACAGGCTTAATAGTTGACCTTTATGATTGGCTCTGCTACATTTTAAA
CCGATACACACTAAATCGAT

Product: AMP-dependent synthetase and ligase

Products: D-carnitinyl-CoA; crotonobetainyl-CoA; L-carnitinyl-CoA [C]

Alternate protein names: Medium-chain acyl-CoA synthetase [H]

Number of amino acids: Translated: 556; Mature: 555

Protein sequence:

>556_residues
MSNIYHSAPSAYDFPLLVKQLLNRAKTVSQDQEIVYADKKRFTYKDLFNRINRLANVLDGLNLDAGDVVAVMDWDSHRYL
ESYFAVPMSEYILQTVNIRLSPDKILYTINHAKPKVLLLNSEFAPLVKDYQFENSSIEHIIWLDDNGVTSEGVFGGNQNR
VKGEYEALLDAADDHFDFPDFDENTIATTFYTSGTTGDPKGVFFSHRQLVLHTLTEAASIGMLPNKQGVSYGDVYMPMTP
MFHVHAWGFPFTATMTGLKQVYPGRYAPDLLMDLIINEKVSITHCVPTILQMVLKEAQDRGASFNGLKMIIGGSRLTEGL
AKAAMDQGIEVYTGYGMSETAPLISLTDFSLNEPQLSEEEDIARRCMTGKPVLMVDAQVWGENNQPVGTGQDNTGELVLR
APWLTQSYLKNDDAGKELWENGYMHTQDIAYIRPDGYIKITDRLKDVIKSGGEWISSLEIETILSLHPSVADVSVIGVRD
KQWGERPLALVVLKPNCQDTKVDDIKALAEQAVARGIIPKYGIPSQFKFVDELPKTSVGKHDKKVMREMYANQSEV

Sequences:

>Translated_556_residues
MSNIYHSAPSAYDFPLLVKQLLNRAKTVSQDQEIVYADKKRFTYKDLFNRINRLANVLDGLNLDAGDVVAVMDWDSHRYL
ESYFAVPMSEYILQTVNIRLSPDKILYTINHAKPKVLLLNSEFAPLVKDYQFENSSIEHIIWLDDNGVTSEGVFGGNQNR
VKGEYEALLDAADDHFDFPDFDENTIATTFYTSGTTGDPKGVFFSHRQLVLHTLTEAASIGMLPNKQGVSYGDVYMPMTP
MFHVHAWGFPFTATMTGLKQVYPGRYAPDLLMDLIINEKVSITHCVPTILQMVLKEAQDRGASFNGLKMIIGGSRLTEGL
AKAAMDQGIEVYTGYGMSETAPLISLTDFSLNEPQLSEEEDIARRCMTGKPVLMVDAQVWGENNQPVGTGQDNTGELVLR
APWLTQSYLKNDDAGKELWENGYMHTQDIAYIRPDGYIKITDRLKDVIKSGGEWISSLEIETILSLHPSVADVSVIGVRD
KQWGERPLALVVLKPNCQDTKVDDIKALAEQAVARGIIPKYGIPSQFKFVDELPKTSVGKHDKKVMREMYANQSEV
>Mature_555_residues
SNIYHSAPSAYDFPLLVKQLLNRAKTVSQDQEIVYADKKRFTYKDLFNRINRLANVLDGLNLDAGDVVAVMDWDSHRYLE
SYFAVPMSEYILQTVNIRLSPDKILYTINHAKPKVLLLNSEFAPLVKDYQFENSSIEHIIWLDDNGVTSEGVFGGNQNRV
KGEYEALLDAADDHFDFPDFDENTIATTFYTSGTTGDPKGVFFSHRQLVLHTLTEAASIGMLPNKQGVSYGDVYMPMTPM
FHVHAWGFPFTATMTGLKQVYPGRYAPDLLMDLIINEKVSITHCVPTILQMVLKEAQDRGASFNGLKMIIGGSRLTEGLA
KAAMDQGIEVYTGYGMSETAPLISLTDFSLNEPQLSEEEDIARRCMTGKPVLMVDAQVWGENNQPVGTGQDNTGELVLRA
PWLTQSYLKNDDAGKELWENGYMHTQDIAYIRPDGYIKITDRLKDVIKSGGEWISSLEIETILSLHPSVADVSVIGVRDK
QWGERPLALVVLKPNCQDTKVDDIKALAEQAVARGIIPKYGIPSQFKFVDELPKTSVGKHDKKVMREMYANQSEV

Specific function: Could Catalyze The Transfer Of CoA To Crotonobetaine Or Carnitine. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI187761345, Length=597, Percent_Identity=23.4505862646566, Blast_Score=111, Evalue=2e-24,
Organism=Homo sapiens, GI187761343, Length=597, Percent_Identity=23.4505862646566, Blast_Score=111, Evalue=2e-24,
Organism=Homo sapiens, GI42544132, Length=532, Percent_Identity=23.6842105263158, Blast_Score=111, Evalue=2e-24,
Organism=Homo sapiens, GI156151445, Length=549, Percent_Identity=23.6794171220401, Blast_Score=104, Evalue=2e-22,
Organism=Homo sapiens, GI122937307, Length=527, Percent_Identity=24.8576850094877, Blast_Score=98, Evalue=2e-20,
Organism=Homo sapiens, GI38505220, Length=517, Percent_Identity=23.5976789168279, Blast_Score=95, Evalue=2e-19,
Organism=Homo sapiens, GI28416953, Length=561, Percent_Identity=23.1729055258467, Blast_Score=92, Evalue=1e-18,
Organism=Homo sapiens, GI13375727, Length=541, Percent_Identity=24.0295748613678, Blast_Score=91, Evalue=2e-18,
Organism=Homo sapiens, GI115511026, Length=529, Percent_Identity=22.8733459357278, Blast_Score=84, Evalue=4e-16,
Organism=Homo sapiens, GI58082049, Length=515, Percent_Identity=22.3300970873786, Blast_Score=78, Evalue=2e-14,
Organism=Escherichia coli, GI221142682, Length=514, Percent_Identity=23.9299610894942, Blast_Score=146, Evalue=3e-36,
Organism=Escherichia coli, GI1788107, Length=552, Percent_Identity=25.7246376811594, Blast_Score=139, Evalue=7e-34,
Organism=Escherichia coli, GI145693145, Length=523, Percent_Identity=23.7093690248566, Blast_Score=133, Evalue=4e-32,
Organism=Escherichia coli, GI1786810, Length=555, Percent_Identity=24.1441441441441, Blast_Score=122, Evalue=8e-29,
Organism=Escherichia coli, GI1789201, Length=373, Percent_Identity=24.1286863270777, Blast_Score=82, Evalue=1e-16,
Organism=Escherichia coli, GI1788595, Length=317, Percent_Identity=23.3438485804416, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI1786529, Length=552, Percent_Identity=25, Blast_Score=69, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17559526, Length=384, Percent_Identity=26.8229166666667, Blast_Score=133, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI17531443, Length=501, Percent_Identity=24.3512974051896, Blast_Score=119, Evalue=3e-27,
Organism=Caenorhabditis elegans, GI71994690, Length=571, Percent_Identity=24.6935201401051, Blast_Score=114, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI71994694, Length=571, Percent_Identity=24.6935201401051, Blast_Score=114, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI71994703, Length=571, Percent_Identity=24.6935201401051, Blast_Score=114, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI17538037, Length=524, Percent_Identity=23.2824427480916, Blast_Score=113, Evalue=3e-25,
Organism=Caenorhabditis elegans, GI17560140, Length=524, Percent_Identity=23.2824427480916, Blast_Score=109, Evalue=4e-24,
Organism=Caenorhabditis elegans, GI71996755, Length=526, Percent_Identity=23.7642585551331, Blast_Score=106, Evalue=3e-23,
Organism=Caenorhabditis elegans, GI17557194, Length=551, Percent_Identity=23.049001814882, Blast_Score=106, Evalue=3e-23,
Organism=Caenorhabditis elegans, GI17558820, Length=522, Percent_Identity=24.3295019157088, Blast_Score=105, Evalue=9e-23,
Organism=Caenorhabditis elegans, GI71985884, Length=432, Percent_Identity=22.6851851851852, Blast_Score=101, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI17560308, Length=563, Percent_Identity=24.5115452930728, Blast_Score=93, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI32563687, Length=543, Percent_Identity=21.5469613259669, Blast_Score=93, Evalue=4e-19,
Organism=Saccharomyces cerevisiae, GI6319699, Length=387, Percent_Identity=28.1653746770026, Blast_Score=124, Evalue=4e-29,
Organism=Saccharomyces cerevisiae, GI6319264, Length=570, Percent_Identity=25.4385964912281, Blast_Score=88, Evalue=4e-18,
Organism=Saccharomyces cerevisiae, GI6323182, Length=396, Percent_Identity=23.7373737373737, Blast_Score=69, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6319591, Length=590, Percent_Identity=22.7118644067797, Blast_Score=67, Evalue=8e-12,
Organism=Drosophila melanogaster, GI21355181, Length=569, Percent_Identity=24.780316344464, Blast_Score=133, Evalue=3e-31,
Organism=Drosophila melanogaster, GI18859661, Length=512, Percent_Identity=24.609375, Blast_Score=119, Evalue=5e-27,
Organism=Drosophila melanogaster, GI24581924, Length=590, Percent_Identity=25.2542372881356, Blast_Score=115, Evalue=5e-26,
Organism=Drosophila melanogaster, GI21356947, Length=556, Percent_Identity=22.6618705035971, Blast_Score=93, Evalue=6e-19,
Organism=Drosophila melanogaster, GI19922652, Length=556, Percent_Identity=22.1223021582734, Blast_Score=92, Evalue=1e-18,
Organism=Drosophila melanogaster, GI20130357, Length=370, Percent_Identity=24.0540540540541, Blast_Score=87, Evalue=3e-17,
Organism=Drosophila melanogaster, GI24653035, Length=570, Percent_Identity=21.4035087719298, Blast_Score=81, Evalue=2e-15,
Organism=Drosophila melanogaster, GI21355225, Length=563, Percent_Identity=22.9129662522202, Blast_Score=78, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24648260, Length=378, Percent_Identity=22.4867724867725, Blast_Score=78, Evalue=2e-14,
Organism=Drosophila melanogaster, GI161076582, Length=555, Percent_Identity=20.9009009009009, Blast_Score=74, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24648257, Length=564, Percent_Identity=22.8723404255319, Blast_Score=73, Evalue=6e-13,
Organism=Drosophila melanogaster, GI24656500, Length=537, Percent_Identity=21.6014897579143, Blast_Score=71, Evalue=2e-12,
Organism=Drosophila melanogaster, GI281365686, Length=329, Percent_Identity=22.4924012158055, Blast_Score=68, Evalue=2e-11,
Organism=Drosophila melanogaster, GI21358303, Length=341, Percent_Identity=23.1671554252199, Blast_Score=67, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: 6.3.2.- [C]

Molecular weight: Translated: 62259; Mature: 62127

Theoretical pI: Translated: 4.90; Mature: 4.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNIYHSAPSAYDFPLLVKQLLNRAKTVSQDQEIVYADKKRFTYKDLFNRINRLANVLDG
CCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHCC
LNLDAGDVVAVMDWDSHRYLESYFAVPMSEYILQTVNIRLSPDKILYTINHAKPKVLLLN
CCCCCCCEEEEEECCCHHHHHHHHCCCHHHHHHHHHEEEECCCEEEEEEECCCCEEEEEC
SEFAPLVKDYQFENSSIEHIIWLDDNGVTSEGVFGGNQNRVKGEYEALLDAADDHFDFPD
CCCCHHHHHCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCC
FDENTIATTFYTSGTTGDPKGVFFSHRQLVLHTLTEAASIGMLPNKQGVSYGDVYMPMTP
CCCCCEEEEEEECCCCCCCCCEEHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEECCCCC
MFHVHAWGFPFTATMTGLKQVYPGRYAPDLLMDLIINEKVSITHCVPTILQMVLKEAQDR
CEEEEECCCCHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHC
GASFNGLKMIIGGSRLTEGLAKAAMDQGIEVYTGYGMSETAPLISLTDFSLNEPQLSEEE
CCCCCCEEEEECCHHHHHHHHHHHHHCCCEEEECCCCCCCCCEEEEECCCCCCCCCCCHH
DIARRCMTGKPVLMVDAQVWGENNQPVGTGQDNTGELVLRAPWLTQSYLKNDDAGKELWE
HHHHHHCCCCCEEEEEHHCCCCCCCCCCCCCCCCCCEEEECCCHHHHHHCCCCHHHHHHH
NGYMHTQDIAYIRPDGYIKITDRLKDVIKSGGEWISSLEIETILSLHPSVADVSVIGVRD
CCCEECCCEEEECCCCCEEEHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCEEEEEECC
KQWGERPLALVVLKPNCQDTKVDDIKALAEQAVARGIIPKYGIPSQFKFVDELPKTSVGK
CCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCCCCC
HDKKVMREMYANQSEV
HHHHHHHHHHCCCCCC
>Mature Secondary Structure 
SNIYHSAPSAYDFPLLVKQLLNRAKTVSQDQEIVYADKKRFTYKDLFNRINRLANVLDG
CCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHCC
LNLDAGDVVAVMDWDSHRYLESYFAVPMSEYILQTVNIRLSPDKILYTINHAKPKVLLLN
CCCCCCCEEEEEECCCHHHHHHHHCCCHHHHHHHHHEEEECCCEEEEEEECCCCEEEEEC
SEFAPLVKDYQFENSSIEHIIWLDDNGVTSEGVFGGNQNRVKGEYEALLDAADDHFDFPD
CCCCHHHHHCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCC
FDENTIATTFYTSGTTGDPKGVFFSHRQLVLHTLTEAASIGMLPNKQGVSYGDVYMPMTP
CCCCCEEEEEEECCCCCCCCCEEHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEECCCCC
MFHVHAWGFPFTATMTGLKQVYPGRYAPDLLMDLIINEKVSITHCVPTILQMVLKEAQDR
CEEEEECCCCHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHC
GASFNGLKMIIGGSRLTEGLAKAAMDQGIEVYTGYGMSETAPLISLTDFSLNEPQLSEEE
CCCCCCEEEEECCHHHHHHHHHHHHHCCCEEEECCCCCCCCCEEEEECCCCCCCCCCCHH
DIARRCMTGKPVLMVDAQVWGENNQPVGTGQDNTGELVLRAPWLTQSYLKNDDAGKELWE
HHHHHHCCCCCEEEEEHHCCCCCCCCCCCCCCCCCCEEEECCCHHHHHHCCCCHHHHHHH
NGYMHTQDIAYIRPDGYIKITDRLKDVIKSGGEWISSLEIETILSLHPSVADVSVIGVRD
CCCEECCCEEEECCCCCEEEHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCEEEEEECC
KQWGERPLALVVLKPNCQDTKVDDIKALAEQAVARGIIPKYGIPSQFKFVDELPKTSVGK
CCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCCCCC
HDKKVMREMYANQSEV
HHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Coenzyme A; D-carnitine; Coenzyme A; crotono-betaine; L-carnitine [C]

Specific reaction: Coenzyme A + D-carnitine = D-carnitinyl-CoA Coenzyme A + crotono-betaine = crotonobetainyl-CoA Coenzyme A + L-carnitine = L-carnitinyl-CoA [C]

General reaction: Ligases; Forming Carbon-Sulfur Bonds; Acid-Thiol Ligases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1453953 [H]