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The map label for this gene is mmsB [H]

Identifier: 93006339

GI number: 93006339

Start: 1859921

End: 1860886

Strand: Reverse

Name: mmsB [H]

Synonym: Pcryo_1513

Alternate gene names: 93006339

Gene position: 1860886-1859921 (Counterclockwise)

Preceding gene: 93006340

Following gene: 93006337

Centisome position: 60.82

GC content: 46.17

Gene sequence:

>966_bases
ATGGCTACAAAGGATACAGGTTCAAGTAGTACAACTACGACTGATAATGATGGTAATACAGCAAAGCCAAATATCGCCTT
TATTGGACTAGGTAACATGGGCGGTCCAATGGCAGCAAACTTGTTAAAGGCAGGTTATACGCTATCGGTTTATGATTTAT
CTGTAGAGGCGACCGCGCGCTTGCAACAAGCGGGTGCCAATATAGCAGAGAGTCCGAAAGATGCTGCTCAAAATGCCCAA
GTGATTATCAGTATGTTACCTGCTGGCAAGCACGTGCATTCTGTCTATCTAGGAGAAGGTAGCTCTGACGGTCTACTTGT
AGACTTACCAAAAGGTACTTTGGTTATCGATAGCAGTACCATAGCAGCAGCTGATGCTAGGAAAGTAGCAGAAGTTGCAA
GTAAGCTTGGTATTGATTTTTTAGATGCGCCAGTCTCTGGCGGTACAGGTGGCGCTATCGCTGGCACATTGACCTTTATC
GTTGGTGGAGACGATGCAGCCTTTGCCAAAGCTGAACCGATACTGGCTGTAATGGGAAAAAATATCTTCCACGCAGGCGA
GCATGGTGCAGGACAAGTAGCTAAAATCTGTAATAACATGCTGCTTGGAATTCTGATGGCAGGAACGGCAGAGGCGATTA
ATCTAGGTGTCAAAAATGGTCTCGATCCAAAGGTACTATCCGATATCATGCTACAAAGCTCCGGTCGTAACTGGACGCTA
GAGGTCTACAACCCGTACCCGCAAGTGATGGAAAATGTCCCATCGAGCAATGGCTATCAAGGCGGCTTTATGAGTAAGCT
GATGCAAAAGGATCTTAACCTTGCCATGCAAACCGCTCAAGACACGCATGTAGAGACACCAATGGGCGCTAAAGCAACTG
AGCTATATGCTGCGCATACCATCGAGAATGGTGATAGAGATTTTTCTAGTATTATGGCGCGTCACGACAGCTCTGTATTG
TCTTAA

Upstream 100 bases:

>100_bases
GCATCTGATTTAAGCTAACTTAACTGGATTAAGTGACTAGATTAAGTGAAATTTGAAAAATTAAAACTAATTGCATAATC
AATCATCAAGGAGCGATGAG

Downstream 100 bases:

>100_bases
ATTATTAAGCACACAAATAAAAAAGGTCGGCATTATAGTAATAGTGCAGACCTTTTTTTGACTTATTTCTAGTTTATATC
AAGGCATTTAACTTGGCAGC

Product: 3-hydroxyisobutyrate dehydrogenase

Products: NA

Alternate protein names: HIBADH [H]

Number of amino acids: Translated: 321; Mature: 320

Protein sequence:

>321_residues
MATKDTGSSSTTTTDNDGNTAKPNIAFIGLGNMGGPMAANLLKAGYTLSVYDLSVEATARLQQAGANIAESPKDAAQNAQ
VIISMLPAGKHVHSVYLGEGSSDGLLVDLPKGTLVIDSSTIAAADARKVAEVASKLGIDFLDAPVSGGTGGAIAGTLTFI
VGGDDAAFAKAEPILAVMGKNIFHAGEHGAGQVAKICNNMLLGILMAGTAEAINLGVKNGLDPKVLSDIMLQSSGRNWTL
EVYNPYPQVMENVPSSNGYQGGFMSKLMQKDLNLAMQTAQDTHVETPMGAKATELYAAHTIENGDRDFSSIMARHDSSVL
S

Sequences:

>Translated_321_residues
MATKDTGSSSTTTTDNDGNTAKPNIAFIGLGNMGGPMAANLLKAGYTLSVYDLSVEATARLQQAGANIAESPKDAAQNAQ
VIISMLPAGKHVHSVYLGEGSSDGLLVDLPKGTLVIDSSTIAAADARKVAEVASKLGIDFLDAPVSGGTGGAIAGTLTFI
VGGDDAAFAKAEPILAVMGKNIFHAGEHGAGQVAKICNNMLLGILMAGTAEAINLGVKNGLDPKVLSDIMLQSSGRNWTL
EVYNPYPQVMENVPSSNGYQGGFMSKLMQKDLNLAMQTAQDTHVETPMGAKATELYAAHTIENGDRDFSSIMARHDSSVL
S
>Mature_320_residues
ATKDTGSSSTTTTDNDGNTAKPNIAFIGLGNMGGPMAANLLKAGYTLSVYDLSVEATARLQQAGANIAESPKDAAQNAQV
IISMLPAGKHVHSVYLGEGSSDGLLVDLPKGTLVIDSSTIAAADARKVAEVASKLGIDFLDAPVSGGTGGAIAGTLTFIV
GGDDAAFAKAEPILAVMGKNIFHAGEHGAGQVAKICNNMLLGILMAGTAEAINLGVKNGLDPKVLSDIMLQSSGRNWTLE
VYNPYPQVMENVPSSNGYQGGFMSKLMQKDLNLAMQTAQDTHVETPMGAKATELYAAHTIENGDRDFSSIMARHDSSVLS

Specific function: D-galactarate metabolism; third step. [C]

COG id: COG2084

COG function: function code I; 3-hydroxyisobutyrate dehydrogenase and related beta-hydroxyacid dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 3-hydroxyisobutyrate dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI23308751, Length=293, Percent_Identity=48.122866894198, Blast_Score=271, Evalue=4e-73,
Organism=Homo sapiens, GI40556376, Length=317, Percent_Identity=28.7066246056782, Blast_Score=119, Evalue=3e-27,
Organism=Escherichia coli, GI145693186, Length=277, Percent_Identity=38.9891696750903, Blast_Score=167, Evalue=8e-43,
Organism=Escherichia coli, GI1786719, Length=274, Percent_Identity=34.6715328467153, Blast_Score=149, Evalue=2e-37,
Organism=Escherichia coli, GI1790315, Length=291, Percent_Identity=37.4570446735395, Blast_Score=148, Evalue=6e-37,
Organism=Escherichia coli, GI1789092, Length=292, Percent_Identity=29.7945205479452, Blast_Score=106, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI17557316, Length=271, Percent_Identity=48.7084870848708, Blast_Score=243, Evalue=1e-64,
Organism=Drosophila melanogaster, GI24655230, Length=289, Percent_Identity=42.9065743944637, Blast_Score=226, Evalue=2e-59,
Organism=Drosophila melanogaster, GI19922568, Length=289, Percent_Identity=42.9065743944637, Blast_Score=226, Evalue=2e-59,
Organism=Drosophila melanogaster, GI28574115, Length=323, Percent_Identity=24.1486068111455, Blast_Score=86, Evalue=4e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002204
- InterPro:   IPR015815
- InterPro:   IPR008927
- InterPro:   IPR006115
- InterPro:   IPR013328
- InterPro:   IPR011548
- InterPro:   IPR016040 [H]

Pfam domain/function: PF03446 NAD_binding_2 [H]

EC number: =1.1.1.31 [H]

Molecular weight: Translated: 33172; Mature: 33041

Theoretical pI: Translated: 4.82; Mature: 4.82

Prosite motif: PS00895 3_HYDROXYISOBUT_DH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATKDTGSSSTTTTDNDGNTAKPNIAFIGLGNMGGPMAANLLKAGYTLSVYDLSVEATAR
CCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHCCCEEEEEEECHHHHHH
LQQAGANIAESPKDAAQNAQVIISMLPAGKHVHSVYLGEGSSDGLLVDLPKGTLVIDSST
HHHHCCCCCCCCHHHHCCCEEEEEECCCCCCEEEEEECCCCCCCEEEECCCCEEEEECCC
IAAADARKVAEVASKLGIDFLDAPVSGGTGGAIAGTLTFIVGGDDAAFAKAEPILAVMGK
CCHHHHHHHHHHHHHHCCEEECCCCCCCCCCEEEEEEEEEECCCCCCHHCCCCHHHHHCC
NIFHAGEHGAGQVAKICNNMLLGILMAGTAEAINLGVKNGLDPKVLSDIMLQSSGRNWTL
HHEECCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCCHHHHHHHHHHCCCCEEEE
EVYNPYPQVMENVPSSNGYQGGFMSKLMQKDLNLAMQTAQDTHVETPMGAKATELYAAHT
EEECCCHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHEEHH
IENGDRDFSSIMARHDSSVLS
CCCCCHHHHHHHHHCCHHHCC
>Mature Secondary Structure 
ATKDTGSSSTTTTDNDGNTAKPNIAFIGLGNMGGPMAANLLKAGYTLSVYDLSVEATAR
CCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHCCCEEEEEEECHHHHHH
LQQAGANIAESPKDAAQNAQVIISMLPAGKHVHSVYLGEGSSDGLLVDLPKGTLVIDSST
HHHHCCCCCCCCHHHHCCCEEEEEECCCCCCEEEEEECCCCCCCEEEECCCCEEEEECCC
IAAADARKVAEVASKLGIDFLDAPVSGGTGGAIAGTLTFIVGGDDAAFAKAEPILAVMGK
CCHHHHHHHHHHHHHHCCEEECCCCCCCCCCEEEEEEEEEECCCCCCHHCCCCHHHHHCC
NIFHAGEHGAGQVAKICNNMLLGILMAGTAEAINLGVKNGLDPKVLSDIMLQSSGRNWTL
HHEECCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCCHHHHHHHHHHCCCCEEEE
EVYNPYPQVMENVPSSNGYQGGFMSKLMQKDLNLAMQTAQDTHVETPMGAKATELYAAHT
EEECCCHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHEEHH
IENGDRDFSSIMARHDSSVLS
CCCCCHHHHHHHHHCCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1339433; 10984043 [H]