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The map label for this gene is aroF [H]

Identifier: 93006333

GI number: 93006333

Start: 1851926

End: 1853008

Strand: Direct

Name: aroF [H]

Synonym: Pcryo_1507

Alternate gene names: 93006333

Gene position: 1851926-1853008 (Clockwise)

Preceding gene: 93006329

Following gene: 93006335

Centisome position: 60.52

GC content: 45.06

Gene sequence:

>1083_bases
ATGATAACAACCGCTACCCATAATGCAGAAATTGACGACGTGAATATTGAAAAATTTATTCCATTAATCACACCCAATGA
GCTAAAAACCGAACTTCCTTTATCAAACGAAGCCTATAAAACCGTTTTAAATGGTCGTCACACTATCCAAAACATCTTGG
ATGGCAAAGACAAGCGTTTGTTTGTGGTTATTGGTCCATGCTCTATTCATGACATCAAAGCCGCTCATGAATATGCTGAT
CGCTTAGCCATATTGGCGAAAGAGATTGAAGACAGCGTATTCGTTGTGATGCGTGTCTATTTTGAGAAGCCGCGCACGAC
CGTTGGCTGGAAAGGCATGATTAATGACCCTGATATGAATGACAGCTTTGATATCGAAAAAGGTCTGCGTACAGCCCGTA
AGCTACTGCTTGATCTCAATGAAAAAGGACTACCTTGTGCTACTGAAGCGCTTGACCCGAATACACCGCAGTACATGCAG
GACTTGATCAGCTGGTCAGCGATTGGCGCACGTACGACCGAGAGCCAAACGCATCGTGAGATGAGCTCAGGCTTATCATG
CCCAGTCGGCTTTAAAAACGGTACTGATGGTGGCATGACTGTTGCGGTCAACGCCATGCAAGCGGTAAAAGCGGGTCATA
GCTTCTTGGGTCTGTCCTCAGATGGCAAAGTGTCTATCATCAAATCTAAAGGTAACCCTTATGCTCACGTGGTATTACGC
GGTGGCAATGGTAAGCCTAACTACGATGAAACAGCAGTCGCTCAAGTAGAAGACGCGCTTGCCAAAGGTAAAACTAGTAG
CAAGATTATGATTGACTCAAGTCATGCCAACTCTGGTAAAGACCCTTATTTGCAACCGATGGTGATCCAAAACGTTGCGG
AGCAAATCAGAAATGGTAACAAGTCCATCATTGGTATGATGATTGAGAGTCACTTAAAAGGCGGTCGCCAAGAGCTGACT
GCTGATTTGGATGCGCTCGAATACGGCATGTCAATTACAGATGGTTGCCTAGATTGGGACAGCACGGTCACAGCACTACA
TAACTTACGTGATATGGTCAAAGACATCCTACCTAACCGTTAA

Upstream 100 bases:

>100_bases
TTCCAGTTATCAGTCAATTAACTGCTCGACTCCCCTTGTCAACTGGTACTTTTGTCCGCATAATGAAAGTCCCTATAAAT
CAGCTAGACGATATATAATC

Downstream 100 bases:

>100_bases
TAACTGCTTATCCAATAATAGCTGCTTATTAAAAAAGCCCGTTTGACACTATATTGTCAGACGGGCTTTTTTCATGTTTA
AGATTATAATTTTAGATTTC

Product: phospho-2-dehydro-3-deoxyheptonate aldolase

Products: NA

Alternate protein names: 3-deoxy-D-arabino-heptulosonate 7-phosphate synthase; DAHP synthase; Phospho-2-keto-3-deoxyheptonate aldolase [H]

Number of amino acids: Translated: 360; Mature: 360

Protein sequence:

>360_residues
MITTATHNAEIDDVNIEKFIPLITPNELKTELPLSNEAYKTVLNGRHTIQNILDGKDKRLFVVIGPCSIHDIKAAHEYAD
RLAILAKEIEDSVFVVMRVYFEKPRTTVGWKGMINDPDMNDSFDIEKGLRTARKLLLDLNEKGLPCATEALDPNTPQYMQ
DLISWSAIGARTTESQTHREMSSGLSCPVGFKNGTDGGMTVAVNAMQAVKAGHSFLGLSSDGKVSIIKSKGNPYAHVVLR
GGNGKPNYDETAVAQVEDALAKGKTSSKIMIDSSHANSGKDPYLQPMVIQNVAEQIRNGNKSIIGMMIESHLKGGRQELT
ADLDALEYGMSITDGCLDWDSTVTALHNLRDMVKDILPNR

Sequences:

>Translated_360_residues
MITTATHNAEIDDVNIEKFIPLITPNELKTELPLSNEAYKTVLNGRHTIQNILDGKDKRLFVVIGPCSIHDIKAAHEYAD
RLAILAKEIEDSVFVVMRVYFEKPRTTVGWKGMINDPDMNDSFDIEKGLRTARKLLLDLNEKGLPCATEALDPNTPQYMQ
DLISWSAIGARTTESQTHREMSSGLSCPVGFKNGTDGGMTVAVNAMQAVKAGHSFLGLSSDGKVSIIKSKGNPYAHVVLR
GGNGKPNYDETAVAQVEDALAKGKTSSKIMIDSSHANSGKDPYLQPMVIQNVAEQIRNGNKSIIGMMIESHLKGGRQELT
ADLDALEYGMSITDGCLDWDSTVTALHNLRDMVKDILPNR
>Mature_360_residues
MITTATHNAEIDDVNIEKFIPLITPNELKTELPLSNEAYKTVLNGRHTIQNILDGKDKRLFVVIGPCSIHDIKAAHEYAD
RLAILAKEIEDSVFVVMRVYFEKPRTTVGWKGMINDPDMNDSFDIEKGLRTARKLLLDLNEKGLPCATEALDPNTPQYMQ
DLISWSAIGARTTESQTHREMSSGLSCPVGFKNGTDGGMTVAVNAMQAVKAGHSFLGLSSDGKVSIIKSKGNPYAHVVLR
GGNGKPNYDETAVAQVEDALAKGKTSSKIMIDSSHANSGKDPYLQPMVIQNVAEQIRNGNKSIIGMMIESHLKGGRQELT
ADLDALEYGMSITDGCLDWDSTVTALHNLRDMVKDILPNR

Specific function: Stereospecific condensation of phosphoenolpyruvate (PEP) and D-erythrose-4-phosphate (E4P) giving rise to 3-deoxy-D- arabino-heptulosonate-7-phosphate (DAHP) [H]

COG id: COG0722

COG function: function code E; 3-deoxy-D-arabino-heptulosonate 7-phosphate (DAHP) synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I DAHP synthase family [H]

Homologues:

Organism=Escherichia coli, GI1788953, Length=346, Percent_Identity=53.1791907514451, Blast_Score=383, Evalue=1e-108,
Organism=Escherichia coli, GI1786969, Length=347, Percent_Identity=53.0259365994236, Blast_Score=362, Evalue=1e-101,
Organism=Escherichia coli, GI1787996, Length=342, Percent_Identity=48.2456140350877, Blast_Score=337, Evalue=7e-94,
Organism=Saccharomyces cerevisiae, GI6319726, Length=344, Percent_Identity=49.1279069767442, Blast_Score=341, Evalue=1e-94,
Organism=Saccharomyces cerevisiae, GI6320240, Length=357, Percent_Identity=45.6582633053221, Blast_Score=333, Evalue=3e-92,

Paralogues:

None

Copy number: 2,200 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006218
- InterPro:   IPR006219 [H]

Pfam domain/function: PF00793 DAHP_synth_1 [H]

EC number: =2.5.1.54 [H]

Molecular weight: Translated: 39424; Mature: 39424

Theoretical pI: Translated: 6.01; Mature: 6.01

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MITTATHNAEIDDVNIEKFIPLITPNELKTELPLSNEAYKTVLNGRHTIQNILDGKDKRL
CCCCCCCCCCCCCCCHHHHCCCCCCCCHHCCCCCCCHHHHHHHCCHHHHHHHHCCCCCEE
FVVIGPCSIHDIKAAHEYADRLAILAKEIEDSVFVVMRVYFEKPRTTVGWKGMINDPDMN
EEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCC
DSFDIEKGLRTARKLLLDLNEKGLPCATEALDPNTPQYMQDLISWSAIGARTTESQTHRE
CCCCHHHHHHHHHHHHHHCCCCCCCCCHHCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHH
MSSGLSCPVGFKNGTDGGMTVAVNAMQAVKAGHSFLGLSSDGKVSIIKSKGNPYAHVVLR
HHCCCCCCCCCCCCCCCCEEEEHHHHHHHHCCCCEEECCCCCCEEEEECCCCCEEEEEEE
GGNGKPNYDETAVAQVEDALAKGKTSSKIMIDSSHANSGKDPYLQPMVIQNVAEQIRNGN
CCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHCCC
KSIIGMMIESHLKGGRQELTADLDALEYGMSITDGCLDWDSTVTALHNLRDMVKDILPNR
HHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MITTATHNAEIDDVNIEKFIPLITPNELKTELPLSNEAYKTVLNGRHTIQNILDGKDKRL
CCCCCCCCCCCCCCCHHHHCCCCCCCCHHCCCCCCCHHHHHHHCCHHHHHHHHCCCCCEE
FVVIGPCSIHDIKAAHEYADRLAILAKEIEDSVFVVMRVYFEKPRTTVGWKGMINDPDMN
EEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCC
DSFDIEKGLRTARKLLLDLNEKGLPCATEALDPNTPQYMQDLISWSAIGARTTESQTHRE
CCCCHHHHHHHHHHHHHHCCCCCCCCCHHCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHH
MSSGLSCPVGFKNGTDGGMTVAVNAMQAVKAGHSFLGLSSDGKVSIIKSKGNPYAHVVLR
HHCCCCCCCCCCCCCCCCEEEEHHHHHHHHCCCCEEECCCCCCEEEEECCCCCEEEEEEE
GGNGKPNYDETAVAQVEDALAKGKTSSKIMIDSSHANSGKDPYLQPMVIQNVAEQIRNGN
CCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHCCC
KSIIGMMIESHLKGGRQELTADLDALEYGMSITDGCLDWDSTVTALHNLRDMVKDILPNR
HHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 6146618; 6396419; 9205837; 9278503; 1977738; 6104668; 2857723; 9056491 [H]