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The map label for this gene is radA [H]

Identifier: 93006313

GI number: 93006313

Start: 1830373

End: 1831818

Strand: Reverse

Name: radA [H]

Synonym: Pcryo_1487

Alternate gene names: 93006313

Gene position: 1831818-1830373 (Counterclockwise)

Preceding gene: 93006314

Following gene: 93006310

Centisome position: 59.87

GC content: 48.2

Gene sequence:

>1446_bases
ATGGCAAAAAATAAAACCAGTTATGTCTGTCAAGCTTGCGGTGCTAATTTTGGCAAGTGGGCAGGGCAATGCAGTGATTG
CGGCGAATGGAATACCTTGACTGAAGCGCCTAACGTCAGTATGCCACATCATAATAAAAACGTAGCAAAGCCCAATGCTG
GTCGCGCCATACCTCGTAATGCAAGTGGTGCTGGTACACCGGCGGGCAATTATGCAGGAACGCATAGTGCTGTGATGCCG
CTCAATGCGGTGAGCGTAGGTTTAGATACTAGGTTGCCGACGGGTATTAGTGAATTTGATCGTGTGTTGGGCGGTGGATT
GGTTGCAGGTTCTGTGGTACTGATTGGTGGTGATCCCGGTATCGGAAAATCGACCATTCTATTGCAAACCGCGACCAATA
TGGCAAAAGCGGATTCACTGGCAGGAAGCGCTTTGTATGTGACTGGTGAGGAATCATTGGCACAGGTTGCTATGCGGGCA
AAGCGCTTGGATTTGCCTGCGGACAGACTTCGTGTGCTTGCTGAAACCAATGTCGAGACTATTTGTGCTGCCTTGACTCA
AGAGCAGCCAGCAATTGCTATTATTGATTCTATTCAGACTATTTATACTGATGCGATTAACTCTGCACCAGGCGGGGTCA
GTCAAATTCGAGAGTCAGCGGCGATTTTGACGCGTTATGCTAAGCAAACAGGTACAGCGCTGTTTCTAGTGGGACATGTC
ACCAAAGAAGGCACGCTTGCTGGTCCACGCGTTCTTGAGCATATGGTCGATACGGTATTGTACTTTGAGGGTCAGTCTGA
TTCGCGTTTTCGTATGATTCGTGCCGTTAAAAACCGTTTTGGCGCGGTCAATGAGCTAGGTATTTTTGGCATGACGGATA
TGGGACTAAAAGAGGTTGCCAATCCATCAGCGATATTTTTGAGCCGCTATGATAAGCCCGTGGCGGGTTCTGTGGTTATG
GTCAGCCGAGAAGGCACAAGACCGCTATTGGTAGAGGTACAAGCCTTAGTTGATGACTCGCAAGGCTCACCAAGACGTAT
GGCATTAGGATTAGACTTTCAGCGCTTGTCTATGCTGTTAGCAGTGATGCATCGCCATGGCGGTATCCATACCAGCGGGC
AAGATGTCTATGTCAACGTTGTTGGCGGTGTCAAAGTCATAGAAACAGGGTCAGACTTGGCAGTATTATTAGCCTGTGCA
TCTAGCATTAGAGAAAAAGCCTTGCCATCGTCGTTGGCGGTCTTTGGTGAAGTGGGGTTATCAGGAGAAATTCGTCCTGT
ACCCAATGGACAAGAGCGTTTAAAGGAAGCCATGAAGCATGGTTTTAAGCATGCCATTATTCCTAAAGCCAATGCGCCTG
CCAAAGATGCGCCGCAGTTTAAAGGCATTAATGTCATTGCAGTCGAACGTCTTGATGATGCGATTGCGAGTGCATTCGAG
TTATAG

Upstream 100 bases:

>100_bases
TATACTTTAGTTCTTATCTTCAAAAATACGCTTATAAGCGTCATTCCCTTTCATATACTCTCTCTTTTAAACTTGAACAC
CTGCATAAGGCTTCTATATT

Downstream 100 bases:

>100_bases
TTTGTTGATCTAACATTTATAACTATAAACCTTATAAATTTAAGACACGAAAAAGCGCCTAACTATCAATTAGTTAGGCG
CTTTTAGTTAAATCTAAAAA

Product: DNA repair protein RadA

Products: NA

Alternate protein names: DNA repair protein sms homolog [H]

Number of amino acids: Translated: 481; Mature: 480

Protein sequence:

>481_residues
MAKNKTSYVCQACGANFGKWAGQCSDCGEWNTLTEAPNVSMPHHNKNVAKPNAGRAIPRNASGAGTPAGNYAGTHSAVMP
LNAVSVGLDTRLPTGISEFDRVLGGGLVAGSVVLIGGDPGIGKSTILLQTATNMAKADSLAGSALYVTGEESLAQVAMRA
KRLDLPADRLRVLAETNVETICAALTQEQPAIAIIDSIQTIYTDAINSAPGGVSQIRESAAILTRYAKQTGTALFLVGHV
TKEGTLAGPRVLEHMVDTVLYFEGQSDSRFRMIRAVKNRFGAVNELGIFGMTDMGLKEVANPSAIFLSRYDKPVAGSVVM
VSREGTRPLLVEVQALVDDSQGSPRRMALGLDFQRLSMLLAVMHRHGGIHTSGQDVYVNVVGGVKVIETGSDLAVLLACA
SSIREKALPSSLAVFGEVGLSGEIRPVPNGQERLKEAMKHGFKHAIIPKANAPAKDAPQFKGINVIAVERLDDAIASAFE
L

Sequences:

>Translated_481_residues
MAKNKTSYVCQACGANFGKWAGQCSDCGEWNTLTEAPNVSMPHHNKNVAKPNAGRAIPRNASGAGTPAGNYAGTHSAVMP
LNAVSVGLDTRLPTGISEFDRVLGGGLVAGSVVLIGGDPGIGKSTILLQTATNMAKADSLAGSALYVTGEESLAQVAMRA
KRLDLPADRLRVLAETNVETICAALTQEQPAIAIIDSIQTIYTDAINSAPGGVSQIRESAAILTRYAKQTGTALFLVGHV
TKEGTLAGPRVLEHMVDTVLYFEGQSDSRFRMIRAVKNRFGAVNELGIFGMTDMGLKEVANPSAIFLSRYDKPVAGSVVM
VSREGTRPLLVEVQALVDDSQGSPRRMALGLDFQRLSMLLAVMHRHGGIHTSGQDVYVNVVGGVKVIETGSDLAVLLACA
SSIREKALPSSLAVFGEVGLSGEIRPVPNGQERLKEAMKHGFKHAIIPKANAPAKDAPQFKGINVIAVERLDDAIASAFE
L
>Mature_480_residues
AKNKTSYVCQACGANFGKWAGQCSDCGEWNTLTEAPNVSMPHHNKNVAKPNAGRAIPRNASGAGTPAGNYAGTHSAVMPL
NAVSVGLDTRLPTGISEFDRVLGGGLVAGSVVLIGGDPGIGKSTILLQTATNMAKADSLAGSALYVTGEESLAQVAMRAK
RLDLPADRLRVLAETNVETICAALTQEQPAIAIIDSIQTIYTDAINSAPGGVSQIRESAAILTRYAKQTGTALFLVGHVT
KEGTLAGPRVLEHMVDTVLYFEGQSDSRFRMIRAVKNRFGAVNELGIFGMTDMGLKEVANPSAIFLSRYDKPVAGSVVMV
SREGTRPLLVEVQALVDDSQGSPRRMALGLDFQRLSMLLAVMHRHGGIHTSGQDVYVNVVGGVKVIETGSDLAVLLACAS
SIREKALPSSLAVFGEVGLSGEIRPVPNGQERLKEAMKHGFKHAIIPKANAPAKDAPQFKGINVIAVERLDDAIASAFEL

Specific function: May play a role in the repair of endogenous alkylation damage [H]

COG id: COG1066

COG function: function code O; Predicted ATP-dependent serine protease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the recA family. RadA subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790850, Length=480, Percent_Identity=54.7916666666667, Blast_Score=513, Evalue=1e-147,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR014774
- InterPro:   IPR004504
- InterPro:   IPR008269
- InterPro:   IPR020568 [H]

Pfam domain/function: PF06745 KaiC; PF05362 Lon_C [H]

EC number: NA

Molecular weight: Translated: 50679; Mature: 50548

Theoretical pI: Translated: 8.14; Mature: 8.14

Prosite motif: PS50162 RECA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKNKTSYVCQACGANFGKWAGQCSDCGEWNTLTEAPNVSMPHHNKNVAKPNAGRAIPRN
CCCCCHHHHHHHHCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
ASGAGTPAGNYAGTHSAVMPLNAVSVGLDTRLPTGISEFDRVLGGGLVAGSVVLIGGDPG
CCCCCCCCCCCCCCCCCCCCCHHEEECCCCCCCCCHHHHHHHHCCCEEECEEEEEECCCC
IGKSTILLQTATNMAKADSLAGSALYVTGEESLAQVAMRAKRLDLPADRLRVLAETNVET
CCCCEEEEEEHHHHHHHHHCCCCEEEEECHHHHHHHHHHHHHCCCCHHHHHHHHHCCHHH
ICAALTQEQPAIAIIDSIQTIYTDAINSAPGGVSQIRESAAILTRYAKQTGTALFLVGHV
HHHHHHCCCCCEEHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEEEC
TKEGTLAGPRVLEHMVDTVLYFEGQSDSRFRMIRAVKNRFGAVNELGIFGMTDMGLKEVA
CCCCCCCHHHHHHHHHHHHEEECCCCCCHHHHHHHHHHHHCCHHHCCCCCCCCCCHHHHC
NPSAIFLSRYDKPVAGSVVMVSREGTRPLLVEVQALVDDSQGSPRRMALGLDFQRLSMLL
CCCEEEEECCCCCCCCCEEEEECCCCCCCEEEEEEHHCCCCCCCCEEEECCCHHHHHHHH
AVMHRHGGIHTSGQDVYVNVVGGVKVIETGSDLAVLLACASSIREKALPSSLAVFGEVGL
HHHHHCCCCCCCCCEEEEEEECCEEEEECCCCHHHHHHHHHHHHHHHCCHHHHHHHHCCC
SGEIRPVPNGQERLKEAMKHGFKHAIIPKANAPAKDAPQFKGINVIAVERLDDAIASAFE
CCCCCCCCCHHHHHHHHHHCCCCEEECCCCCCCCCCCCCCCCCEEEEHHHHHHHHHHHHC
L
C
>Mature Secondary Structure 
AKNKTSYVCQACGANFGKWAGQCSDCGEWNTLTEAPNVSMPHHNKNVAKPNAGRAIPRN
CCCCHHHHHHHHCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
ASGAGTPAGNYAGTHSAVMPLNAVSVGLDTRLPTGISEFDRVLGGGLVAGSVVLIGGDPG
CCCCCCCCCCCCCCCCCCCCCHHEEECCCCCCCCCHHHHHHHHCCCEEECEEEEEECCCC
IGKSTILLQTATNMAKADSLAGSALYVTGEESLAQVAMRAKRLDLPADRLRVLAETNVET
CCCCEEEEEEHHHHHHHHHCCCCEEEEECHHHHHHHHHHHHHCCCCHHHHHHHHHCCHHH
ICAALTQEQPAIAIIDSIQTIYTDAINSAPGGVSQIRESAAILTRYAKQTGTALFLVGHV
HHHHHHCCCCCEEHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEEEC
TKEGTLAGPRVLEHMVDTVLYFEGQSDSRFRMIRAVKNRFGAVNELGIFGMTDMGLKEVA
CCCCCCCHHHHHHHHHHHHEEECCCCCCHHHHHHHHHHHHCCHHHCCCCCCCCCCHHHHC
NPSAIFLSRYDKPVAGSVVMVSREGTRPLLVEVQALVDDSQGSPRRMALGLDFQRLSMLL
CCCEEEEECCCCCCCCCEEEEECCCCCCCEEEEEEHHCCCCCCCCEEEECCCHHHHHHHH
AVMHRHGGIHTSGQDVYVNVVGGVKVIETGSDLAVLLACASSIREKALPSSLAVFGEVGL
HHHHHCCCCCCCCCEEEEEEECCEEEEECCCCHHHHHHHHHHHHHHHCCHHHHHHHHCCC
SGEIRPVPNGQERLKEAMKHGFKHAIIPKANAPAKDAPQFKGINVIAVERLDDAIASAFE
CCCCCCCCCHHHHHHHHHHCCCCEEECCCCCCCCCCCCCCCCCEEEEHHHHHHHHHHHHC
L
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]