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The map label for this gene is ogt [H]

Identifier: 93006306

GI number: 93006306

Start: 1820187

End: 1820759

Strand: Reverse

Name: ogt [H]

Synonym: Pcryo_1480

Alternate gene names: 93006306

Gene position: 1820759-1820187 (Counterclockwise)

Preceding gene: 93006307

Following gene: 93006304

Centisome position: 59.5

GC content: 42.76

Gene sequence:

>573_bases
ATGATAGTCACCACAGCCGCTTTTGGATCTAATCAGTTAACATTAATTGCGAGTGTCAATGAGCATAGCAGTCCTAAGCT
GGTTGAGGTCAATTGGCTGCTGGCAGGTGACTCTTGGCACAGCTCAAAATCTATTCCCAAGCTTAAAAAGCATTATGGTA
TTGACGATCAAAGCTTCACATTCATAGATAAAGACAGCCTAAGCAAAAATGAACCTACTCAAGCATTATTAATAGAGGCT
ATAGCACAATTAACGGAGTATTTTAACGGTGAGCGTCAAGCGTTCGATTTGCCACTAGATGCAAGCTTAGGAACTAAGTT
TCAGCAGCGAGTTTGGCAAGCACTACAAGATATTGCTTATGGTGAGACGATTAGCTATGCAACGCTGGCACAAAATGTCG
ATAGTCCCAAAGGCTTTCGTGCGGTTGCGAATGCCAATAGTAAAAACCCTTTTAGTATTATCGTTCCGTGTCATCGTGTT
ATTGCAAGCGACGGCAAACTTGGTGGTTATACAGGCGGCTTAGATAAGAAAACGCATTTGTTAGCGCTTGAAGGCGTCAC
GTGCAAAGCTTAG

Upstream 100 bases:

>100_bases
TCGCCGCGTTGTTTGAAACTGATGACAAAGAATAGCGCTCAACAGTTTATTTTAGCCAATACTCAAGTCTTGAAAATACA
ACGATTTGAAAGGAGTAGTT

Downstream 100 bases:

>100_bases
TTTGAAGTAATTGCTCGTCACATGTCACATATTGTTGAACAAAAAAGCCAAACGCTAAAAACGTTTGGCTTTTTTAATAC
AAAGCACAAAAATTACTGCT

Product: methylated-DNA--protein-cysteine methyltransferase

Products: NA

Alternate protein names: 6-O-methylguanine-DNA methyltransferase; MGMT; O-6-methylguanine-DNA-alkyltransferase [H]

Number of amino acids: Translated: 190; Mature: 190

Protein sequence:

>190_residues
MIVTTAAFGSNQLTLIASVNEHSSPKLVEVNWLLAGDSWHSSKSIPKLKKHYGIDDQSFTFIDKDSLSKNEPTQALLIEA
IAQLTEYFNGERQAFDLPLDASLGTKFQQRVWQALQDIAYGETISYATLAQNVDSPKGFRAVANANSKNPFSIIVPCHRV
IASDGKLGGYTGGLDKKTHLLALEGVTCKA

Sequences:

>Translated_190_residues
MIVTTAAFGSNQLTLIASVNEHSSPKLVEVNWLLAGDSWHSSKSIPKLKKHYGIDDQSFTFIDKDSLSKNEPTQALLIEA
IAQLTEYFNGERQAFDLPLDASLGTKFQQRVWQALQDIAYGETISYATLAQNVDSPKGFRAVANANSKNPFSIIVPCHRV
IASDGKLGGYTGGLDKKTHLLALEGVTCKA
>Mature_190_residues
MIVTTAAFGSNQLTLIASVNEHSSPKLVEVNWLLAGDSWHSSKSIPKLKKHYGIDDQSFTFIDKDSLSKNEPTQALLIEA
IAQLTEYFNGERQAFDLPLDASLGTKFQQRVWQALQDIAYGETISYATLAQNVDSPKGFRAVANANSKNPFSIIVPCHRV
IASDGKLGGYTGGLDKKTHLLALEGVTCKA

Specific function: Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a sui

COG id: COG0350

COG function: function code L; Methylated DNA-protein cysteine methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MGMT family [H]

Homologues:

Organism=Homo sapiens, GI197304670, Length=115, Percent_Identity=40, Blast_Score=86, Evalue=2e-17,
Organism=Escherichia coli, GI1788542, Length=116, Percent_Identity=43.1034482758621, Blast_Score=102, Evalue=1e-23,
Organism=Escherichia coli, GI1787596, Length=103, Percent_Identity=44.6601941747573, Blast_Score=97, Evalue=6e-22,
Organism=Caenorhabditis elegans, GI115533068, Length=81, Percent_Identity=48.1481481481481, Blast_Score=82, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI115533070, Length=81, Percent_Identity=48.1481481481481, Blast_Score=81, Evalue=3e-16,
Organism=Saccharomyces cerevisiae, GI6320001, Length=85, Percent_Identity=34.1176470588235, Blast_Score=73, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001497
- InterPro:   IPR014048
- InterPro:   IPR008332
- InterPro:   IPR011991 [H]

Pfam domain/function: PF01035 DNA_binding_1; PF02870 Methyltransf_1N [H]

EC number: =2.1.1.63 [H]

Molecular weight: Translated: 20698; Mature: 20698

Theoretical pI: Translated: 7.06; Mature: 7.06

Prosite motif: PS00374 MGMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIVTTAAFGSNQLTLIASVNEHSSPKLVEVNWLLAGDSWHSSKSIPKLKKHYGIDDQSFT
CEEEEEECCCCCEEEEEECCCCCCCCEEEEEEEEECCCCCCCCCCHHHHHHCCCCCCCEE
FIDKDSLSKNEPTQALLIEAIAQLTEYFNGERQAFDLPLDASLGTKFQQRVWQALQDIAY
EEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHC
GETISYATLAQNVDSPKGFRAVANANSKNPFSIIVPCHRVIASDGKLGGYTGGLDKKTHL
CCCCHHHHHHHCCCCCCCEEEEECCCCCCCEEEEEEHHHHHHCCCCCCCCCCCCCCCEEE
LALEGVTCKA
EEECCCEECC
>Mature Secondary Structure
MIVTTAAFGSNQLTLIASVNEHSSPKLVEVNWLLAGDSWHSSKSIPKLKKHYGIDDQSFT
CEEEEEECCCCCEEEEEECCCCCCCCEEEEEEEEECCCCCCCCCCHHHHHHCCCCCCCEE
FIDKDSLSKNEPTQALLIEAIAQLTEYFNGERQAFDLPLDASLGTKFQQRVWQALQDIAY
EEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHC
GETISYATLAQNVDSPKGFRAVANANSKNPFSIIVPCHRVIASDGKLGGYTGGLDKKTHL
CCCCHHHHHHHCCCCCCCEEEEECCCCCCCEEEEEEHHHHHHCCCCCCCCCCCCCCCEEE
LALEGVTCKA
EEECCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11234002 [H]