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The map label for this gene is cspA [H]

Identifier: 93006301

GI number: 93006301

Start: 1815379

End: 1815594

Strand: Reverse

Name: cspA [H]

Synonym: Pcryo_1475

Alternate gene names: 93006301

Gene position: 1815594-1815379 (Counterclockwise)

Preceding gene: 93006304

Following gene: 93006300

Centisome position: 59.34

GC content: 42.13

Gene sequence:

>216_bases
ATGTCAGCTCGCGAGCAAGGTATCGTTAAGTGGTTTAATGACTCAAAAGGCTTTGGTTTCATTCAACGTGATAGCGGAGA
AGATATTTTTGTGCATTTCCGCGCGATCCAAGGTGATGGTTATCGCTCTCTAAAAGACGGCGAAAAAGTTGAGTTCAGTG
TAGTAGAAGGTGATAAAGGCCTACAAGCTGAAGAAGTCAGAAAAGTAGAAGAGTAA

Upstream 100 bases:

>100_bases
ATAGTCACTTTTAAGTTTTGATTTCATCATCTTGGTACCCGTTTATATGCAATGATGCAAAGGAATTTGGGACTAAGTAA
ACTTTTTAGGAAGTAATATT

Downstream 100 bases:

>100_bases
TCGTAACAGTCATTCGTGCAGTTTAGCTATAATGTCTGTACAAACTACTGATATACTACTGAGTCAAGCTCGTCCTGTCT
TACTTATGTTGATTTTAACG

Product: cold-shock DNA-binding domain-containing protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 71; Mature: 70

Protein sequence:

>71_residues
MSAREQGIVKWFNDSKGFGFIQRDSGEDIFVHFRAIQGDGYRSLKDGEKVEFSVVEGDKGLQAEEVRKVEE

Sequences:

>Translated_71_residues
MSAREQGIVKWFNDSKGFGFIQRDSGEDIFVHFRAIQGDGYRSLKDGEKVEFSVVEGDKGLQAEEVRKVEE
>Mature_70_residues
SAREQGIVKWFNDSKGFGFIQRDSGEDIFVHFRAIQGDGYRSLKDGEKVEFSVVEGDKGLQAEEVRKVEE

Specific function: Binds To And Stimulates The Transcription Of The CCAAT- Containing, Cold-Shock-Inducible Promoters Of The H-Ns And Gyra Proteins. Binds Also To The Inverted Repeat 5'-ATTGG-3'. [C]

COG id: COG1278

COG function: function code K; Cold shock proteins

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CSD (cold-shock) domain [H]

Homologues:

Organism=Homo sapiens, GI34098946, Length=65, Percent_Identity=53.8461538461538, Blast_Score=65, Evalue=9e-12,
Organism=Homo sapiens, GI156415990, Length=65, Percent_Identity=53.8461538461538, Blast_Score=64, Evalue=3e-11,
Organism=Escherichia coli, GI1789979, Length=70, Percent_Identity=55.7142857142857, Blast_Score=80, Evalue=2e-17,
Organism=Escherichia coli, GI1787107, Length=67, Percent_Identity=55.2238805970149, Blast_Score=77, Evalue=2e-16,
Organism=Escherichia coli, GI1787839, Length=67, Percent_Identity=55.2238805970149, Blast_Score=76, Evalue=5e-16,
Organism=Escherichia coli, GI2367114, Length=67, Percent_Identity=52.2388059701493, Blast_Score=75, Evalue=1e-15,
Organism=Escherichia coli, GI1788126, Length=68, Percent_Identity=51.4705882352941, Blast_Score=74, Evalue=2e-15,
Organism=Escherichia coli, GI1786841, Length=65, Percent_Identity=50.7692307692308, Blast_Score=73, Evalue=5e-15,
Organism=Escherichia coli, GI1787834, Length=67, Percent_Identity=47.7611940298507, Blast_Score=70, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17533635, Length=66, Percent_Identity=53.030303030303, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019844
- InterPro:   IPR012156
- InterPro:   IPR011129
- InterPro:   IPR002059
- InterPro:   IPR012340
- InterPro:   IPR016027 [H]

Pfam domain/function: PF00313 CSD [H]

EC number: NA

Molecular weight: Translated: 8083; Mature: 7952

Theoretical pI: Translated: 4.64; Mature: 4.64

Prosite motif: PS00352 COLD_SHOCK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
0.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAREQGIVKWFNDSKGFGFIQRDSGEDIFVHFRAIQGDGYRSLKDGEKVEFSVVEGDKG
CCCCCCCEEEEECCCCCCEEEECCCCCEEEEEEEEECCCCCCCCCCCCEEEEEEEECCCC
LQAEEVRKVEE
CCHHHHHCCCC
>Mature Secondary Structure 
SAREQGIVKWFNDSKGFGFIQRDSGEDIFVHFRAIQGDGYRSLKDGEKVEFSVVEGDKG
CCCCCCEEEEECCCCCCEEEECCCCCEEEEEEEEECCCCCCCCCCCCEEEEEEEECCCC
LQAEEVRKVEE
CCHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]