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The map label for this gene is dnaB [H]

Identifier: 93006285

GI number: 93006285

Start: 1799553

End: 1800956

Strand: Direct

Name: dnaB [H]

Synonym: Pcryo_1459

Alternate gene names: 93006285

Gene position: 1799553-1800956 (Clockwise)

Preceding gene: 93006283

Following gene: 93006286

Centisome position: 58.81

GC content: 43.8

Gene sequence:

>1404_bases
ATGGCAGATACTGAAAAAACCGACGACTTACTCAATCAAACACCACCGCACAATATCGATATTGAAAAGGCGCTGCTGGC
GTCTTTGATGAGTATCGAAGAGGCATACGATAAGATTGCAGATATCGTTACCAAAGACGATTTTTATGCTGGGCGACATC
AGTATATTTTTGATGCTATTGCGCATTTAGCTGCAGTCAATGAGCCTTATGACACGGTTATGGTACATGATTGGTTAGAG
GCACAAAAGCTGCTAAAAAGCGCAGGCGGCGACAGCTATTTGGCGGATATCTTGAGCCAAAGTCCAGCAACTTTGTTTAA
CCTGACGGCTTACGCCCAGCGTGTACGCGAGCTATCTACACTACGTCAGCTGATTACTACCGGCAATGATATGTTGACCC
TAGCCTATAATCCCAAAGACCAAACCGTCAGCGATATTTTGGATAACGTAGAAGCGAAGATATTTAGCATCAATGAGCAG
CATAATAAACGTGCAGAGCAGCGTGGACCTGTCGGTATTACTGCCGTATTGACCAATGTCATTGATAAAATCCAAGAGCT
CAAATCAAATCCAGATGGCATGATTGGACTACAAACCCCATTTGTTGAGCTCAATAATAAAACCCAAGGCTTGCAAGCAG
GCGATTTAGTCATTGTGGCTGCGCGTCCATCAATGGGTAAAACCACCTTTGCGATGAACTTAGCAGAAGGGATTTTATTT
AATGAAGATTTGCCTGTAGTGGTATTTTCGATGGAAATGCCCGCAGAATCTATCGTGATGCGTTTGCTATCATCTTGGGG
CGCAATCAACCAAACACATCTGCGTTCTGGGCAAATGAATGAAGATGAATGGGCAAAAATGATGAACGCCATTCAGCATT
TGCAGTCAAAGCATCTATATATTGATGACAGTACTGCCCTGCCACCCTCTGAGCTGCGTTCACGCTGCCGCCGTATTGCC
AAAAATCATGATGGCAAACTTGGCGCTATCATTGTCGATTATCTACAATTAATGAAAGTACCCGGTCTAGATGGCAACCG
TGTTGGGGAAATCTCTGAGATTTCTCGAAGTTTAAAAGCCCTAGCACGTGAGCTTGAATGTCCAGTTATCGCCTTGTCGC
AGCTTAACCGTAGTCTAGAAAATCGTCCTAACAAACGTCCTATTATGTCGGATCTGCGTGAATCTGGTGCGATTGAGCAG
GATGCCGACTTGATTATGTTCATCTATCGTGATGAGGTTTATAATGAAAACTCAGACCACAAAGGTGTGGCTGAAATCAT
TATTGGTAAGCAGCGTAACGGCCCTATCGGCACCATACGTTTGGGTTTTGAAGGACAATTTACCCGCTTTATCAATTTGA
CGCCAGAATACTATCAAAATGTCAGTTTTGAGAACGACGAATAA

Upstream 100 bases:

>100_bases
CTCTCATCGAAATGCATTGCGCTTGCTTTGCTGTATCATTACTAAAATAAATACTTACAACAATACCCATCTATACCGAC
TTTTGAAATATTAGGCACTC

Downstream 100 bases:

>100_bases
TAAAACTGACAGATAATTACTCATCAAAATTGCTCATAAAAATGGCCAGTGACTCATCAACTCAATTGATGAATTACTGG
CTTTATAAATTTATTCCTTC

Product: replicative DNA helicase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 467; Mature: 466

Protein sequence:

>467_residues
MADTEKTDDLLNQTPPHNIDIEKALLASLMSIEEAYDKIADIVTKDDFYAGRHQYIFDAIAHLAAVNEPYDTVMVHDWLE
AQKLLKSAGGDSYLADILSQSPATLFNLTAYAQRVRELSTLRQLITTGNDMLTLAYNPKDQTVSDILDNVEAKIFSINEQ
HNKRAEQRGPVGITAVLTNVIDKIQELKSNPDGMIGLQTPFVELNNKTQGLQAGDLVIVAARPSMGKTTFAMNLAEGILF
NEDLPVVVFSMEMPAESIVMRLLSSWGAINQTHLRSGQMNEDEWAKMMNAIQHLQSKHLYIDDSTALPPSELRSRCRRIA
KNHDGKLGAIIVDYLQLMKVPGLDGNRVGEISEISRSLKALARELECPVIALSQLNRSLENRPNKRPIMSDLRESGAIEQ
DADLIMFIYRDEVYNENSDHKGVAEIIIGKQRNGPIGTIRLGFEGQFTRFINLTPEYYQNVSFENDE

Sequences:

>Translated_467_residues
MADTEKTDDLLNQTPPHNIDIEKALLASLMSIEEAYDKIADIVTKDDFYAGRHQYIFDAIAHLAAVNEPYDTVMVHDWLE
AQKLLKSAGGDSYLADILSQSPATLFNLTAYAQRVRELSTLRQLITTGNDMLTLAYNPKDQTVSDILDNVEAKIFSINEQ
HNKRAEQRGPVGITAVLTNVIDKIQELKSNPDGMIGLQTPFVELNNKTQGLQAGDLVIVAARPSMGKTTFAMNLAEGILF
NEDLPVVVFSMEMPAESIVMRLLSSWGAINQTHLRSGQMNEDEWAKMMNAIQHLQSKHLYIDDSTALPPSELRSRCRRIA
KNHDGKLGAIIVDYLQLMKVPGLDGNRVGEISEISRSLKALARELECPVIALSQLNRSLENRPNKRPIMSDLRESGAIEQ
DADLIMFIYRDEVYNENSDHKGVAEIIIGKQRNGPIGTIRLGFEGQFTRFINLTPEYYQNVSFENDE
>Mature_466_residues
ADTEKTDDLLNQTPPHNIDIEKALLASLMSIEEAYDKIADIVTKDDFYAGRHQYIFDAIAHLAAVNEPYDTVMVHDWLEA
QKLLKSAGGDSYLADILSQSPATLFNLTAYAQRVRELSTLRQLITTGNDMLTLAYNPKDQTVSDILDNVEAKIFSINEQH
NKRAEQRGPVGITAVLTNVIDKIQELKSNPDGMIGLQTPFVELNNKTQGLQAGDLVIVAARPSMGKTTFAMNLAEGILFN
EDLPVVVFSMEMPAESIVMRLLSSWGAINQTHLRSGQMNEDEWAKMMNAIQHLQSKHLYIDDSTALPPSELRSRCRRIAK
NHDGKLGAIIVDYLQLMKVPGLDGNRVGEISEISRSLKALARELECPVIALSQLNRSLENRPNKRPIMSDLRESGAIEQD
ADLIMFIYRDEVYNENSDHKGVAEIIIGKQRNGPIGTIRLGFEGQFTRFINLTPEYYQNVSFENDE

Specific function: Participates in initiation and elongation during chromosome replication; it exhibits DNA-dependent ATPase activity and contains distinct active sites for ATP binding, DNA binding, and interaction with dnaC protein, primase, and other prepriming proteins [

COG id: COG0305

COG function: function code L; Replicative DNA helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 SF4 helicase domain [H]

Homologues:

Organism=Escherichia coli, GI1790486, Length=448, Percent_Identity=51.3392857142857, Blast_Score=460, Evalue=1e-130,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR007692
- InterPro:   IPR007694
- InterPro:   IPR007693
- InterPro:   IPR016136 [H]

Pfam domain/function: PF00772 DnaB; PF03796 DnaB_C [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 52276; Mature: 52145

Theoretical pI: Translated: 4.75; Mature: 4.75

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADTEKTDDLLNQTPPHNIDIEKALLASLMSIEEAYDKIADIVTKDDFYAGRHQYIFDAI
CCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHH
AHLAAVNEPYDTVMVHDWLEAQKLLKSAGGDSYLADILSQSPATLFNLTAYAQRVRELST
HHHHHCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
LRQLITTGNDMLTLAYNPKDQTVSDILDNVEAKIFSINEQHNKRAEQRGPVGITAVLTNV
HHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHEEEECCHHHHHHHHHCCCCHHHHHHHHH
IDKIQELKSNPDGMIGLQTPFVELNNKTQGLQAGDLVIVAARPSMGKTTFAMNLAEGILF
HHHHHHHHCCCCCEEECCCCEEEECCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHCCEE
NEDLPVVVFSMEMPAESIVMRLLSSWGAINQTHLRSGQMNEDEWAKMMNAIQHLQSKHLY
CCCCCEEEEEECCCHHHHHHHHHHHCCCCCHHHHCCCCCCHHHHHHHHHHHHHHHHCCEE
IDDSTALPPSELRSRCRRIAKNHDGKLGAIIVDYLQLMKVPGLDGNRVGEISEISRSLKA
EECCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHH
LARELECPVIALSQLNRSLENRPNKRPIMSDLRESGAIEQDADLIMFIYRDEVYNENSDH
HHHHHCCCHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCCCEEEEEEEHHHCCCCCCC
KGVAEIIIGKQRNGPIGTIRLGFEGQFTRFINLTPEYYQNVSFENDE
CCHHHHHEECCCCCCEEEEEECCCCCEEEEEECCHHHHHCCCCCCCC
>Mature Secondary Structure 
ADTEKTDDLLNQTPPHNIDIEKALLASLMSIEEAYDKIADIVTKDDFYAGRHQYIFDAI
CCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHH
AHLAAVNEPYDTVMVHDWLEAQKLLKSAGGDSYLADILSQSPATLFNLTAYAQRVRELST
HHHHHCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
LRQLITTGNDMLTLAYNPKDQTVSDILDNVEAKIFSINEQHNKRAEQRGPVGITAVLTNV
HHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHEEEECCHHHHHHHHHCCCCHHHHHHHHH
IDKIQELKSNPDGMIGLQTPFVELNNKTQGLQAGDLVIVAARPSMGKTTFAMNLAEGILF
HHHHHHHHCCCCCEEECCCCEEEECCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHCCEE
NEDLPVVVFSMEMPAESIVMRLLSSWGAINQTHLRSGQMNEDEWAKMMNAIQHLQSKHLY
CCCCCEEEEEECCCHHHHHHHHHHHCCCCCHHHHCCCCCCHHHHHHHHHHHHHHHHCCEE
IDDSTALPPSELRSRCRRIAKNHDGKLGAIIVDYLQLMKVPGLDGNRVGEISEISRSLKA
EECCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHH
LARELECPVIALSQLNRSLENRPNKRPIMSDLRESGAIEQDADLIMFIYRDEVYNENSDH
HHHHHCCCHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCCCEEEEEEEHHHCCCCCCC
KGVAEIIIGKQRNGPIGTIRLGFEGQFTRFINLTPEYYQNVSFENDE
CCHHHHHEECCCCCCEEEEEECCCCCEEEEEECCHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]