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The map label for this gene is btuB [H]

Identifier: 93006273

GI number: 93006273

Start: 1780301

End: 1782199

Strand: Direct

Name: btuB [H]

Synonym: Pcryo_1447

Alternate gene names: 93006273

Gene position: 1780301-1782199 (Clockwise)

Preceding gene: 93006271

Following gene: 93006274

Centisome position: 58.18

GC content: 41.44

Gene sequence:

>1899_bases
ATGGTTTTATCACGTTCATCTTCAACCACTTATCTGCGGTTGTGTATTTTAAGCGCATTTGGCGCATTTGCTATGAGCGC
TAACGCCGCTACTGACGTCAATGTCATCAGTGATAGCGAGCTACCACAAGTTGAGCTTGATAAAATTGTAGTCACCGCCA
CTCGTACACCGACTAAAACCAGCAATGTCATTGCTCAGACTCGCGTCATTGATCAAGAAGACTTGAAACGCTTCCAAGGT
CAAAATGCCCTTGATATCATCAAACGTCAGCCAGGTATCAGTCATTATACCAATGGCGGGCCTGGTACCACCTCAAACTT
TTATATGCGTGGATATGATAATAAGCAAATATTGGTGCTTATTGATGGGGTACGCTATAGCTCAATTAGTGCTGGCGGCG
CAACATTAAGCTTACTGCCTGCTGATCAAATCGATCGTATTGAAATATTATATGGTGCTTCTGGCTCTAGTATTTATGGT
TCAGACGCAATCGGCGGCGTGATTCAAGTGTTTACTAAAGGCTCAAACATTGAAAAGAGTCAGGTTTCTATTACTGCAGG
TATAGGCTCACATAATGAATATCTATATGGCGCTAGTGCTCAACTTAACAGTGGCAATGGCACGACAATGAGTTTATCAG
CCAGCCATAATGAGACGGATGGCATCAATGCGACCTTACCAAATGCCTATGGTTATAATAGCGATGATGACGGCTTTAAA
AGCGATAACGTTAGCTTAGCGTTAACTCAGCGTATCAATGAACAATGGTTAACCGGTGCAAGCGCCTTATATAGCAAATC
GACCACAGATTATGACGATGGTGCAGCAAACGCCTATTCAAAACAAGAAAACGGTTCTGCGCAAGCGTTTGTTGATTGGC
GTTATATGCCTGGTTCTTCTGTCAAATTACAGTATGGTCATTCTATTGATAAGAGTGATAACTACGGTAGCTTCCCTGGT
GTTTTTGATACCAAACAAAATCAAGTTAGCCTAGTAGGTCAGCATAAGTTTCAAGTTGGTCAAGGCATATATGGCCTTGA
ATACCTTAACCAAGGGCTTGATAGTACTGCCTATACCGTTGATGACCGTGACGTGAAAAGCGCATTCTTAGGCTATCTAT
TGGCCAAAAATCAATTCGATGCTCAAGCCAACGTGCGCTTTGATGACAACTCACAATATGGTGGTGAGACGACTTACAAC
CTAGGTGGTGCCTATCACATTAATCCTAACCTACGCGCTGGTGCCAACTACGCCAAAGGCTTTCGTGCGCCTACTTTTAA
TGACTTATACTATCCGGGTTCAGGTAATACTAATTTGAAACCTGAAACCAGCGATAACTATGAAGCTTTTGTTGAATACG
ATACTGTATTACAGTCGACACGTCTAACAGGCTATCACAACAAAGTTGATGATCTAATTGGCTATGTTGCAAAACCTACA
CCTGGCAATCCTTGGGGCGGTAGCAGTGAAAACGTTGATAGAGCTAAAATTGAAGGTATTACCTTAACTTCAGATTGGGT
TGTTGATAGCTATTTATTTGGTGGTAGCTATGACTATCAGCAAGCAAAAAATGCCAGTGGCGGTGCGAGCGATGGTAATT
TCCTTGCCATTCGTCCTGAACATAAAGGTCTGGTATATGCAGGCTACCGCTTACCAAGCTTAGATGTTCGTGCAGAATAC
CAATATGTTGGTGATTATTATAGTGGTGTCGCTAATACAGACTCACAGTTAGTCGATTCTTACGGTTTATTGAATATCAG
CGGTAACTATAAACTCACTGATAATCTATCTATGACTGCTCGTTTAAATAATATTGCGAATAAAAAATATATTACTCTAC
CGGGTTATAATACTGATGGTATTAACTTCTTCACCTCTTTAACCTATAATTGGTTCTAG

Upstream 100 bases:

>100_bases
AGATAATAACGCCATGTATTTTATTTATTACTTTTTGTAATTTTTAGCTGCTCAGATTTCACTTCTGTAACTTCTTTATT
TGATTAATTTGGAATTCAAC

Downstream 100 bases:

>100_bases
GGTGATTTAGAATCACACTGAACTGGTATTATTCATTTAAATGAGAAAAGGTCATCCAACGATGGCCTTTTTTATGGATG
TCAATCACATAAAACGATAT

Product: TonB-dependent receptor

Products: NA

Alternate protein names: Cobalamin receptor; Outer membrane cobalamin translocator [H]

Number of amino acids: Translated: 632; Mature: 632

Protein sequence:

>632_residues
MVLSRSSSTTYLRLCILSAFGAFAMSANAATDVNVISDSELPQVELDKIVVTATRTPTKTSNVIAQTRVIDQEDLKRFQG
QNALDIIKRQPGISHYTNGGPGTTSNFYMRGYDNKQILVLIDGVRYSSISAGGATLSLLPADQIDRIEILYGASGSSIYG
SDAIGGVIQVFTKGSNIEKSQVSITAGIGSHNEYLYGASAQLNSGNGTTMSLSASHNETDGINATLPNAYGYNSDDDGFK
SDNVSLALTQRINEQWLTGASALYSKSTTDYDDGAANAYSKQENGSAQAFVDWRYMPGSSVKLQYGHSIDKSDNYGSFPG
VFDTKQNQVSLVGQHKFQVGQGIYGLEYLNQGLDSTAYTVDDRDVKSAFLGYLLAKNQFDAQANVRFDDNSQYGGETTYN
LGGAYHINPNLRAGANYAKGFRAPTFNDLYYPGSGNTNLKPETSDNYEAFVEYDTVLQSTRLTGYHNKVDDLIGYVAKPT
PGNPWGGSSENVDRAKIEGITLTSDWVVDSYLFGGSYDYQQAKNASGGASDGNFLAIRPEHKGLVYAGYRLPSLDVRAEY
QYVGDYYSGVANTDSQLVDSYGLLNISGNYKLTDNLSMTARLNNIANKKYITLPGYNTDGINFFTSLTYNWF

Sequences:

>Translated_632_residues
MVLSRSSSTTYLRLCILSAFGAFAMSANAATDVNVISDSELPQVELDKIVVTATRTPTKTSNVIAQTRVIDQEDLKRFQG
QNALDIIKRQPGISHYTNGGPGTTSNFYMRGYDNKQILVLIDGVRYSSISAGGATLSLLPADQIDRIEILYGASGSSIYG
SDAIGGVIQVFTKGSNIEKSQVSITAGIGSHNEYLYGASAQLNSGNGTTMSLSASHNETDGINATLPNAYGYNSDDDGFK
SDNVSLALTQRINEQWLTGASALYSKSTTDYDDGAANAYSKQENGSAQAFVDWRYMPGSSVKLQYGHSIDKSDNYGSFPG
VFDTKQNQVSLVGQHKFQVGQGIYGLEYLNQGLDSTAYTVDDRDVKSAFLGYLLAKNQFDAQANVRFDDNSQYGGETTYN
LGGAYHINPNLRAGANYAKGFRAPTFNDLYYPGSGNTNLKPETSDNYEAFVEYDTVLQSTRLTGYHNKVDDLIGYVAKPT
PGNPWGGSSENVDRAKIEGITLTSDWVVDSYLFGGSYDYQQAKNASGGASDGNFLAIRPEHKGLVYAGYRLPSLDVRAEY
QYVGDYYSGVANTDSQLVDSYGLLNISGNYKLTDNLSMTARLNNIANKKYITLPGYNTDGINFFTSLTYNWF
>Mature_632_residues
MVLSRSSSTTYLRLCILSAFGAFAMSANAATDVNVISDSELPQVELDKIVVTATRTPTKTSNVIAQTRVIDQEDLKRFQG
QNALDIIKRQPGISHYTNGGPGTTSNFYMRGYDNKQILVLIDGVRYSSISAGGATLSLLPADQIDRIEILYGASGSSIYG
SDAIGGVIQVFTKGSNIEKSQVSITAGIGSHNEYLYGASAQLNSGNGTTMSLSASHNETDGINATLPNAYGYNSDDDGFK
SDNVSLALTQRINEQWLTGASALYSKSTTDYDDGAANAYSKQENGSAQAFVDWRYMPGSSVKLQYGHSIDKSDNYGSFPG
VFDTKQNQVSLVGQHKFQVGQGIYGLEYLNQGLDSTAYTVDDRDVKSAFLGYLLAKNQFDAQANVRFDDNSQYGGETTYN
LGGAYHINPNLRAGANYAKGFRAPTFNDLYYPGSGNTNLKPETSDNYEAFVEYDTVLQSTRLTGYHNKVDDLIGYVAKPT
PGNPWGGSSENVDRAKIEGITLTSDWVVDSYLFGGSYDYQQAKNASGGASDGNFLAIRPEHKGLVYAGYRLPSLDVRAEY
QYVGDYYSGVANTDSQLVDSYGLLNISGNYKLTDNLSMTARLNNIANKKYITLPGYNTDGINFFTSLTYNWF

Specific function: Involved in the active translocation of vitamin B12 (cyanocobalamin) across the outer membrane to the periplasmic space. It derives its energy for transport by interacting with the trans-periplasmic membrane protein TonB [H]

COG id: COG4206

COG function: function code H; Outer membrane cobalamin receptor protein

Gene ontology:

Cell location: Cell outer membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TonB-dependent receptor family. BtuB (TC 1.B.14.3.1) subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790405, Length=651, Percent_Identity=28.5714285714286, Blast_Score=181, Evalue=1e-46,
Organism=Escherichia coli, GI1788478, Length=646, Percent_Identity=24.4582043343653, Blast_Score=101, Evalue=1e-22,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012910
- InterPro:   IPR000531 [H]

Pfam domain/function: PF07715 Plug; PF00593 TonB_dep_Rec [H]

EC number: NA

Molecular weight: Translated: 68698; Mature: 68698

Theoretical pI: Translated: 4.75; Mature: 4.75

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.1 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVLSRSSSTTYLRLCILSAFGAFAMSANAATDVNVISDSELPQVELDKIVVTATRTPTKT
CEECCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCEEECEEEEEEECCCCCC
SNVIAQTRVIDQEDLKRFQGQNALDIIKRQPGISHYTNGGPGTTSNFYMRGYDNKQILVL
CHHEEEHEECCHHHHHHHCCCCHHHHHHCCCCCCCCCCCCCCCCCCEEEEECCCCEEEEE
IDGVRYSSISAGGATLSLLPADQIDRIEILYGASGSSIYGSDAIGGVIQVFTKGSNIEKS
EECCEEECCCCCCCEEEECCCCCCCEEEEEECCCCCCCCCCCHHCCEEEEEECCCCCCCC
QVSITAGIGSHNEYLYGASAQLNSGNGTTMSLSASHNETDGINATLPNAYGYNSDDDGFK
EEEEEEECCCCCCEEEECCEEEECCCCCEEEEECCCCCCCCCEEECCCCCCCCCCCCCCC
SDNVSLALTQRINEQWLTGASALYSKSTTDYDDGAANAYSKQENGSAQAFVDWRYMPGSS
CCCEEEEEEHHCCHHHHCCHHHHHCCCCCCCCCCCCCCCCCCCCCCEEEEEEEEECCCCC
VKLQYGHSIDKSDNYGSFPGVFDTKQNQVSLVGQHKFQVGQGIYGLEYLNQGLDSTAYTV
EEEEECCCCCCCCCCCCCCCCCCCCCCEEEEEEEEEEECCCCEEHHHHHHCCCCCCEEEE
DDRDVKSAFLGYLLAKNQFDAQANVRFDDNSQYGGETTYNLGGAYHINPNLRAGANYAKG
CCHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCEEEECCEEEEECCCCCCCCCHHCC
FRAPTFNDLYYPGSGNTNLKPETSDNYEAFVEYDTVLQSTRLTGYHNKVDDLIGYVAKPT
CCCCCCCCEEECCCCCCCCCCCCCCCCEEEEEHHHHHHHHCCCCCCCCHHHHHHEECCCC
PGNPWGGSSENVDRAKIEGITLTSDWVVDSYLFGGSYDYQQAKNASGGASDGNFLAIRPE
CCCCCCCCCCCCCCEEEEEEEEECCCEEEEEEECCCCCHHHHCCCCCCCCCCCEEEECCC
HKGLVYAGYRLPSLDVRAEYQYVGDYYSGVANTDSQLVDSYGLLNISGNYKLTDNLSMTA
CCCEEEEEEECCCCEEEEEEEECCHHHCCCCCCCHHHHHCCCEEEECCCEEEECCCEEEE
RLNNIANKKYITLPGYNTDGINFFTSLTYNWF
EHHHCCCCEEEEECCCCCCCCEEEEEEEEECC
>Mature Secondary Structure
MVLSRSSSTTYLRLCILSAFGAFAMSANAATDVNVISDSELPQVELDKIVVTATRTPTKT
CEECCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCEEECEEEEEEECCCCCC
SNVIAQTRVIDQEDLKRFQGQNALDIIKRQPGISHYTNGGPGTTSNFYMRGYDNKQILVL
CHHEEEHEECCHHHHHHHCCCCHHHHHHCCCCCCCCCCCCCCCCCCEEEEECCCCEEEEE
IDGVRYSSISAGGATLSLLPADQIDRIEILYGASGSSIYGSDAIGGVIQVFTKGSNIEKS
EECCEEECCCCCCCEEEECCCCCCCEEEEEECCCCCCCCCCCHHCCEEEEEECCCCCCCC
QVSITAGIGSHNEYLYGASAQLNSGNGTTMSLSASHNETDGINATLPNAYGYNSDDDGFK
EEEEEEECCCCCCEEEECCEEEECCCCCEEEEECCCCCCCCCEEECCCCCCCCCCCCCCC
SDNVSLALTQRINEQWLTGASALYSKSTTDYDDGAANAYSKQENGSAQAFVDWRYMPGSS
CCCEEEEEEHHCCHHHHCCHHHHHCCCCCCCCCCCCCCCCCCCCCCEEEEEEEEECCCCC
VKLQYGHSIDKSDNYGSFPGVFDTKQNQVSLVGQHKFQVGQGIYGLEYLNQGLDSTAYTV
EEEEECCCCCCCCCCCCCCCCCCCCCCEEEEEEEEEEECCCCEEHHHHHHCCCCCCEEEE
DDRDVKSAFLGYLLAKNQFDAQANVRFDDNSQYGGETTYNLGGAYHINPNLRAGANYAKG
CCHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCEEEECCEEEEECCCCCCCCCHHCC
FRAPTFNDLYYPGSGNTNLKPETSDNYEAFVEYDTVLQSTRLTGYHNKVDDLIGYVAKPT
CCCCCCCCEEECCCCCCCCCCCCCCCCEEEEEHHHHHHHHCCCCCCCCHHHHHHEECCCC
PGNPWGGSSENVDRAKIEGITLTSDWVVDSYLFGGSYDYQQAKNASGGASDGNFLAIRPE
CCCCCCCCCCCCCCEEEEEEEEECCCEEEEEEECCCCCHHHHCCCCCCCCCCCEEEECCC
HKGLVYAGYRLPSLDVRAEYQYVGDYYSGVANTDSQLVDSYGLLNISGNYKLTDNLSMTA
CCCEEEEEEECCCCEEEEEEEECCHHHCCCCCCCHHHHHCCCEEEECCCEEEECCCEEEE
RLNNIANKKYITLPGYNTDGINFFTSLTYNWF
EHHHCCCCEEEEECCCCCCCCEEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA