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The map label for this gene is prfA [H]

Identifier: 93006237

GI number: 93006237

Start: 1733061

End: 1734149

Strand: Reverse

Name: prfA [H]

Synonym: Pcryo_1411

Alternate gene names: 93006237

Gene position: 1734149-1733061 (Counterclockwise)

Preceding gene: 93006238

Following gene: 93006236

Centisome position: 56.67

GC content: 45.45

Gene sequence:

>1089_bases
ATGAAAGAATCCTTACGCCTGCGTTTAGACCAAATGGTAGACCGTTATGAAGAGGTCACTGCCTTATTATCAGACCCGAG
TGTCATCAGTGATAACAATAAATTCCGTGAATTGTCCGTCGAACACAGCGACTTGATGGATATCACCACTTTATGGCAGA
ACTATGTTGGTGCAGAAAAGGATCAAGCTGATGCAGAAGCGATGCTAAAAGAAGCATCAGATCCTGACATGAAAGAGATG
ATGCAAGAAGAGATTGACAGTGCTCGCGATACTATCGTAGAGATGGAAGAAGCACTTAATCTCATGATGCTGCCAAAAGA
TCCTAACGATAAAGTGCCCGCATTCCTTGAGATTCGTGCAGGGACAGGTGGTGATGAAGCAGCGATTTTCTCTGGTGATT
TATTCCGCATGTACCAAAAATTCGCCCAGAACCAAGGCTGGACTTTAGAGATTTTGTCAGCCAATGAGGGTGAGCATGGG
GGTTATAAAGAAATCATTACTCGTGTGTCTGGTAATAGCGTTTATGGTCGTCTAAAGTTTGAGTCTGGTGTACATCGTGT
TCAGCGTGTGCCTGATACAGAAAGCCAAGGTCGCGTACATACGTCAGCCTGTACTGTCGCTGTGATGCCTGAAGTTGAAA
TTGATGATACGGTTGATTTGAATCCAGCTGATATTCGCTTTGATACTTTCCGCTCAAGCGGTGCTGGTGGTCAGCACGTG
AATACTACCGACTCTGCTGTACGTTTGACGCATATACCAACGGGTACCGTGGTAGAGTGTCAACAAGAACGCAGTCAACA
TAAAAACCGTGCGCAAGCGATGAAAATGCTGATTTCAAAAATTCAGCAAGTCAAAGTCCAAGCACAGGTAGATGCCGCTG
ATACGATACGTCGCGATTTGGTCGGTAGTGGCGATCGTTCAGAGCGTATCCGCACCTATAACTTCCCGCAAGGACGTATG
ACGGATCATCGTATTAACCTGACTTTATATAAGCTTGACTCGATTATGGAAGGTGACTTGGATGAAATCCTTGATGCGCT
GTTACGTGAGCATCAAGCGGACTTGATGGCAAGTATTGGCGGTGCTTAA

Upstream 100 bases:

>100_bases
TTGTTCATATATTGACAAGATTGCTGAGAGGTTTTAATTTAAGCGCAAAAATTTGCTATAATCACGGTCTTATTTCAATT
ATTCAATTAAAAGTCCTTTT

Downstream 100 bases:

>100_bases
TAAGCACTTCATCAGCCAATAATATAAGAGGCGTACGCCATGCATATGATAACCAACTCATCGCTACTATTACCGTTTAA
TAAAATCGCGATGACTATTG

Product: peptide chain release factor 1

Products: NA

Alternate protein names: RF-1 [H]

Number of amino acids: Translated: 362; Mature: 362

Protein sequence:

>362_residues
MKESLRLRLDQMVDRYEEVTALLSDPSVISDNNKFRELSVEHSDLMDITTLWQNYVGAEKDQADAEAMLKEASDPDMKEM
MQEEIDSARDTIVEMEEALNLMMLPKDPNDKVPAFLEIRAGTGGDEAAIFSGDLFRMYQKFAQNQGWTLEILSANEGEHG
GYKEIITRVSGNSVYGRLKFESGVHRVQRVPDTESQGRVHTSACTVAVMPEVEIDDTVDLNPADIRFDTFRSSGAGGQHV
NTTDSAVRLTHIPTGTVVECQQERSQHKNRAQAMKMLISKIQQVKVQAQVDAADTIRRDLVGSGDRSERIRTYNFPQGRM
TDHRINLTLYKLDSIMEGDLDEILDALLREHQADLMASIGGA

Sequences:

>Translated_362_residues
MKESLRLRLDQMVDRYEEVTALLSDPSVISDNNKFRELSVEHSDLMDITTLWQNYVGAEKDQADAEAMLKEASDPDMKEM
MQEEIDSARDTIVEMEEALNLMMLPKDPNDKVPAFLEIRAGTGGDEAAIFSGDLFRMYQKFAQNQGWTLEILSANEGEHG
GYKEIITRVSGNSVYGRLKFESGVHRVQRVPDTESQGRVHTSACTVAVMPEVEIDDTVDLNPADIRFDTFRSSGAGGQHV
NTTDSAVRLTHIPTGTVVECQQERSQHKNRAQAMKMLISKIQQVKVQAQVDAADTIRRDLVGSGDRSERIRTYNFPQGRM
TDHRINLTLYKLDSIMEGDLDEILDALLREHQADLMASIGGA
>Mature_362_residues
MKESLRLRLDQMVDRYEEVTALLSDPSVISDNNKFRELSVEHSDLMDITTLWQNYVGAEKDQADAEAMLKEASDPDMKEM
MQEEIDSARDTIVEMEEALNLMMLPKDPNDKVPAFLEIRAGTGGDEAAIFSGDLFRMYQKFAQNQGWTLEILSANEGEHG
GYKEIITRVSGNSVYGRLKFESGVHRVQRVPDTESQGRVHTSACTVAVMPEVEIDDTVDLNPADIRFDTFRSSGAGGQHV
NTTDSAVRLTHIPTGTVVECQQERSQHKNRAQAMKMLISKIQQVKVQAQVDAADTIRRDLVGSGDRSERIRTYNFPQGRM
TDHRINLTLYKLDSIMEGDLDEILDALLREHQADLMASIGGA

Specific function: Peptide chain release factor 1 directs the termination of translation in response to the peptide chain termination codons UAG and UAA [H]

COG id: COG0216

COG function: function code J; Protein chain release factor A

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family [H]

Homologues:

Organism=Homo sapiens, GI166795303, Length=313, Percent_Identity=43.4504792332268, Blast_Score=270, Evalue=2e-72,
Organism=Homo sapiens, GI34577120, Length=362, Percent_Identity=37.0165745856354, Blast_Score=231, Evalue=8e-61,
Organism=Homo sapiens, GI166795305, Length=218, Percent_Identity=40.8256880733945, Blast_Score=175, Evalue=7e-44,
Organism=Escherichia coli, GI1787462, Length=359, Percent_Identity=57.6601671309192, Blast_Score=437, Evalue=1e-124,
Organism=Escherichia coli, GI2367172, Length=347, Percent_Identity=37.463976945245, Blast_Score=216, Evalue=1e-57,
Organism=Caenorhabditis elegans, GI17542784, Length=294, Percent_Identity=34.6938775510204, Blast_Score=176, Evalue=1e-44,
Organism=Saccharomyces cerevisiae, GI6321295, Length=284, Percent_Identity=42.9577464788732, Blast_Score=232, Evalue=8e-62,
Organism=Drosophila melanogaster, GI19921226, Length=290, Percent_Identity=40.3448275862069, Blast_Score=215, Evalue=3e-56,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR004373 [H]

Pfam domain/function: PF03462 PCRF; PF00472 RF-1 [H]

EC number: NA

Molecular weight: Translated: 40583; Mature: 40583

Theoretical pI: Translated: 4.55; Mature: 4.55

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
4.7 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKESLRLRLDQMVDRYEEVTALLSDPSVISDNNKFRELSVEHSDLMDITTLWQNYVGAEK
CCCHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCCEEECCCHHHHHHHHHHHHHHCCCCC
DQADAEAMLKEASDPDMKEMMQEEIDSARDTIVEMEEALNLMMLPKDPNDKVPAFLEIRA
CHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCCCCCCCEEEEEEC
GTGGDEAAIFSGDLFRMYQKFAQNQGWTLEILSANEGEHGGYKEIITRVSGNSVYGRLKF
CCCCCCCEEECHHHHHHHHHHHCCCCCEEEEEECCCCCCCCHHHHHHHCCCCCEEEEEEH
ESGVHRVQRVPDTESQGRVHTSACTVAVMPEVEIDDTVDLNPADIRFDTFRSSGAGGQHV
HHHHHHHHCCCCCCCCCEEEECEEEEEEECCCCCCCCCCCCCCCEEEECCCCCCCCCCCC
NTTDSAVRLTHIPTGTVVECQQERSQHKNRAQAMKMLISKIQQVKVQAQVDAADTIRRDL
CCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
VGSGDRSERIRTYNFPQGRMTDHRINLTLYKLDSIMEGDLDEILDALLREHQADLMASIG
CCCCCCCCCCEEECCCCCCCCCCEEEEEEEEEHHHHCCCHHHHHHHHHHHHHHHHHHHCC
GA
CC
>Mature Secondary Structure
MKESLRLRLDQMVDRYEEVTALLSDPSVISDNNKFRELSVEHSDLMDITTLWQNYVGAEK
CCCHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCCEEECCCHHHHHHHHHHHHHHCCCCC
DQADAEAMLKEASDPDMKEMMQEEIDSARDTIVEMEEALNLMMLPKDPNDKVPAFLEIRA
CHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCCCCCCCEEEEEEC
GTGGDEAAIFSGDLFRMYQKFAQNQGWTLEILSANEGEHGGYKEIITRVSGNSVYGRLKF
CCCCCCCEEECHHHHHHHHHHHCCCCCEEEEEECCCCCCCCHHHHHHHCCCCCEEEEEEH
ESGVHRVQRVPDTESQGRVHTSACTVAVMPEVEIDDTVDLNPADIRFDTFRSSGAGGQHV
HHHHHHHHCCCCCCCCCEEEECEEEEEEECCCCCCCCCCCCCCCEEEECCCCCCCCCCCC
NTTDSAVRLTHIPTGTVVECQQERSQHKNRAQAMKMLISKIQQVKVQAQVDAADTIRRDL
CCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
VGSGDRSERIRTYNFPQGRMTDHRINLTLYKLDSIMEGDLDEILDALLREHQADLMASIG
CCCCCCCCCCEEECCCCCCCCCCEEEEEEEEEHHHHCCCHHHHHHHHHHHHHHHHHHHCC
GA
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA