Click here to switch to the map view.

The map label for this gene is lysS [H]

Identifier: 93006235

GI number: 93006235

Start: 1730867

End: 1732411

Strand: Reverse

Name: lysS [H]

Synonym: Pcryo_1409

Alternate gene names: 93006235

Gene position: 1732411-1730867 (Counterclockwise)

Preceding gene: 93006236

Following gene: 93006234

Centisome position: 56.62

GC content: 43.88

Gene sequence:

>1545_bases
ATGTCAAAGCCTAATAATCAAAATCAGCAAAACAATCAAGAGCCAGCTCCAGAAGACGCTAATGAGCTGATTGCCCAATT
GCAAGCCAAGCTGGATGATATCGTCGCTTCAGGTAAACAGCCTTATCCGAATACGTTTAAGCGCACAGATTATGCTCAAG
ACTTGCAGGCAGCTTTTGAGGGTATCTCAAAACAAGAAATTGCTGACAATGATGCTAAAGGCGAAAAAACACAGGTGAAT
GTCGCCGGTCGCGTCATGCTGAACCGTGGGGCGTTTATTGTTATCCAAGATATGACAGGTCGTATCCAGTTATATGTGGC
ACGCAAAGAGTTAGATGAAAAGACGTTAGCAGACATCAAATCGCTAGATTTGGGTGATATCGTTGGTGTATCAGGTTATA
TCGGTCGTTCAGGCAAAGGCGATTTGTACGTACATATTGAAGAGATTGAGTTATTGACCAAAGCATTACGCCCAATGCCA
AATAAATTTCATGGACTAGCTGATGTAGAAGCTCGTTATCGCAATCGTCATCTTGATTTGATGACCAATGAGACGACCCG
TGATACCTTTATGGTGCGCAGTCAAGTCATTAGCGGTATTCGCAAGTTTATGCTAAATGAGCGTTTTATGGAAGTTGAAA
CGCCTATGATGCATCCGATCCCAGGTGGTGCAGTGGCACGTCCGTTTGTGACCCATCATAATGCGCTTGATATGCCATTA
TATCTGCGTATTGCTCCTGAGCTTTATCTTAAGCGCTTGGTCGTTGGTGGTTTTGAAAAAGTCTTTGAGATTAACCGTAG
CTTCCGTAATGAAGGCGTTTCAACCCGTCATAATCCTGAATTTACCATGATTGAGTTCTATCAAGCGTATGCGGATTATC
ATGATTTGATGGATTTGACTGAGCGCTTATTTAATGAATTGGCGACGGATATCTTGGGAACGACTGAGATTACTTACCAA
GAAGAAGCCATTAGCCTAAAAGCGCCATTTATACGTCTATCTATGTCTGATGCGATTGCCAAATATGCAGAAAACTTTGA
TATGAATCGTATTAACGATCGTGAGTATCTTGCAGAGTACGCATCGACAACGCTAAAGCAACAGGTAAAGGATGTCTTTG
GTGTGGGTAAGTTGCAAACCATCATCTTTGAAGAAACCGCTGAGCACCAATTGCGTCAGCCGACGTTTATCACTGAATAC
CCTGCTGAAACCTCACCGCTAGCACGCCGTAGTGATGACAACCCTGAGATTACGGATCGTTTTGAGCTGTTTGTTGGGGG
TCGTGAGTTGGCAAATGGCTTTAGTGAGCTCAATGATCCTGAGGATCAGGCTGAGCGTTTCCTTGGTCAGGTGGCTGAAA
AAGATGCTGGCGATGATGAAGCGATGCATTTCGATGCAGAGTATATCGAAGCTTTGTCTTATGGTTTACCGCCAACAGCA
GGTGAAGGTATTGGTATCGACCGTTTAGTGATGTTATTGACAGACTCTGCCAGTATCCGTGATGTGATTTTATTCCCACA
CATGCGCCGTAAGCTGGAAGGATAA

Upstream 100 bases:

>100_bases
ATTAGGCAGCTGCCTAATTTGAATTTTTTGTTTTAATCCCTTAATTTAAGTTTATCGTTTTCGTTTATTCCTTTAATTAT
CTAATAATTTTGAGAGTGTT

Downstream 100 bases:

>100_bases
AGAAAGATAGTTCGCTTGCCATAAGTCTATCCTAGTACTAAACTGAACATAAGAAGGCTTTTCCGCTTTGATGGAATAGC
CTTCTATGGTTTTTTGGTAC

Product: lysyl-tRNA synthetase

Products: NA

Alternate protein names: Lysine--tRNA ligase; LysRS [H]

Number of amino acids: Translated: 514; Mature: 513

Protein sequence:

>514_residues
MSKPNNQNQQNNQEPAPEDANELIAQLQAKLDDIVASGKQPYPNTFKRTDYAQDLQAAFEGISKQEIADNDAKGEKTQVN
VAGRVMLNRGAFIVIQDMTGRIQLYVARKELDEKTLADIKSLDLGDIVGVSGYIGRSGKGDLYVHIEEIELLTKALRPMP
NKFHGLADVEARYRNRHLDLMTNETTRDTFMVRSQVISGIRKFMLNERFMEVETPMMHPIPGGAVARPFVTHHNALDMPL
YLRIAPELYLKRLVVGGFEKVFEINRSFRNEGVSTRHNPEFTMIEFYQAYADYHDLMDLTERLFNELATDILGTTEITYQ
EEAISLKAPFIRLSMSDAIAKYAENFDMNRINDREYLAEYASTTLKQQVKDVFGVGKLQTIIFEETAEHQLRQPTFITEY
PAETSPLARRSDDNPEITDRFELFVGGRELANGFSELNDPEDQAERFLGQVAEKDAGDDEAMHFDAEYIEALSYGLPPTA
GEGIGIDRLVMLLTDSASIRDVILFPHMRRKLEG

Sequences:

>Translated_514_residues
MSKPNNQNQQNNQEPAPEDANELIAQLQAKLDDIVASGKQPYPNTFKRTDYAQDLQAAFEGISKQEIADNDAKGEKTQVN
VAGRVMLNRGAFIVIQDMTGRIQLYVARKELDEKTLADIKSLDLGDIVGVSGYIGRSGKGDLYVHIEEIELLTKALRPMP
NKFHGLADVEARYRNRHLDLMTNETTRDTFMVRSQVISGIRKFMLNERFMEVETPMMHPIPGGAVARPFVTHHNALDMPL
YLRIAPELYLKRLVVGGFEKVFEINRSFRNEGVSTRHNPEFTMIEFYQAYADYHDLMDLTERLFNELATDILGTTEITYQ
EEAISLKAPFIRLSMSDAIAKYAENFDMNRINDREYLAEYASTTLKQQVKDVFGVGKLQTIIFEETAEHQLRQPTFITEY
PAETSPLARRSDDNPEITDRFELFVGGRELANGFSELNDPEDQAERFLGQVAEKDAGDDEAMHFDAEYIEALSYGLPPTA
GEGIGIDRLVMLLTDSASIRDVILFPHMRRKLEG
>Mature_513_residues
SKPNNQNQQNNQEPAPEDANELIAQLQAKLDDIVASGKQPYPNTFKRTDYAQDLQAAFEGISKQEIADNDAKGEKTQVNV
AGRVMLNRGAFIVIQDMTGRIQLYVARKELDEKTLADIKSLDLGDIVGVSGYIGRSGKGDLYVHIEEIELLTKALRPMPN
KFHGLADVEARYRNRHLDLMTNETTRDTFMVRSQVISGIRKFMLNERFMEVETPMMHPIPGGAVARPFVTHHNALDMPLY
LRIAPELYLKRLVVGGFEKVFEINRSFRNEGVSTRHNPEFTMIEFYQAYADYHDLMDLTERLFNELATDILGTTEITYQE
EAISLKAPFIRLSMSDAIAKYAENFDMNRINDREYLAEYASTTLKQQVKDVFGVGKLQTIIFEETAEHQLRQPTFITEYP
AETSPLARRSDDNPEITDRFELFVGGRELANGFSELNDPEDQAERFLGQVAEKDAGDDEAMHFDAEYIEALSYGLPPTAG
EGIGIDRLVMLLTDSASIRDVILFPHMRRKLEG

Specific function: Unknown

COG id: COG1190

COG function: function code J; Lysyl-tRNA synthetase (class II)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI5031815, Length=519, Percent_Identity=39.4990366088632, Blast_Score=354, Evalue=1e-97,
Organism=Homo sapiens, GI194272210, Length=519, Percent_Identity=39.4990366088632, Blast_Score=354, Evalue=1e-97,
Organism=Homo sapiens, GI45439306, Length=473, Percent_Identity=24.7357293868922, Blast_Score=107, Evalue=3e-23,
Organism=Homo sapiens, GI40789249, Length=282, Percent_Identity=22.6950354609929, Blast_Score=77, Evalue=4e-14,
Organism=Escherichia coli, GI1789256, Length=496, Percent_Identity=57.6612903225806, Blast_Score=572, Evalue=1e-164,
Organism=Escherichia coli, GI1790571, Length=500, Percent_Identity=56, Blast_Score=557, Evalue=1e-160,
Organism=Escherichia coli, GI87082379, Length=332, Percent_Identity=33.1325301204819, Blast_Score=154, Evalue=2e-38,
Organism=Escherichia coli, GI1788173, Length=251, Percent_Identity=25.4980079681275, Blast_Score=67, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17535925, Length=521, Percent_Identity=39.3474088291747, Blast_Score=350, Evalue=7e-97,
Organism=Caenorhabditis elegans, GI17535927, Length=521, Percent_Identity=39.3474088291747, Blast_Score=350, Evalue=9e-97,
Organism=Caenorhabditis elegans, GI71994340, Length=498, Percent_Identity=40.3614457831325, Blast_Score=349, Evalue=2e-96,
Organism=Caenorhabditis elegans, GI17551876, Length=358, Percent_Identity=24.8603351955307, Blast_Score=91, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI32566633, Length=296, Percent_Identity=26.0135135135135, Blast_Score=89, Evalue=5e-18,
Organism=Saccharomyces cerevisiae, GI6320242, Length=494, Percent_Identity=40.4858299595142, Blast_Score=362, Evalue=1e-101,
Organism=Saccharomyces cerevisiae, GI6324256, Length=424, Percent_Identity=31.3679245283019, Blast_Score=196, Evalue=7e-51,
Organism=Saccharomyces cerevisiae, GI6323011, Length=555, Percent_Identity=25.2252252252252, Blast_Score=108, Evalue=2e-24,
Organism=Saccharomyces cerevisiae, GI6321807, Length=456, Percent_Identity=24.780701754386, Blast_Score=94, Evalue=6e-20,
Organism=Drosophila melanogaster, GI24640851, Length=491, Percent_Identity=41.7515274949084, Blast_Score=367, Evalue=1e-101,
Organism=Drosophila melanogaster, GI24640849, Length=491, Percent_Identity=41.7515274949084, Blast_Score=367, Evalue=1e-101,
Organism=Drosophila melanogaster, GI17136276, Length=474, Percent_Identity=24.2616033755274, Blast_Score=100, Evalue=3e-21,
Organism=Drosophila melanogaster, GI24584738, Length=294, Percent_Identity=24.1496598639456, Blast_Score=79, Evalue=1e-14,

Paralogues:

None

Copy number: 1200 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR002313
- InterPro:   IPR018149
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365 [H]

Pfam domain/function: PF00152 tRNA-synt_2; PF01336 tRNA_anti [H]

EC number: =6.1.1.6 [H]

Molecular weight: Translated: 58310; Mature: 58178

Theoretical pI: Translated: 4.64; Mature: 4.64

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKPNNQNQQNNQEPAPEDANELIAQLQAKLDDIVASGKQPYPNTFKRTDYAQDLQAAFE
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHCHHHHHHHHHHH
GISKQEIADNDAKGEKTQVNVAGRVMLNRGAFIVIQDMTGRIQLYVARKELDEKTLADIK
CCCHHHHCCCCCCCCCEEEEEEEEEEEECCCEEEEECCCCCEEEEEEHHHHCHHHHHHHH
SLDLGDIVGVSGYIGRSGKGDLYVHIEEIELLTKALRPMPNKFHGLADVEARYRNRHLDL
CCCCHHHCCCCCCCCCCCCCCEEEEHHHHHHHHHHHCCCCHHHCCCHHHHHHHHCCCEEE
MTNETTRDTFMVRSQVISGIRKFMLNERFMEVETPMMHPIPGGAVARPFVTHHNALDMPL
EECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHCCCCCCCCE
YLRIAPELYLKRLVVGGFEKVFEINRSFRNEGVSTRHNPEFTMIEFYQAYADYHDLMDLT
EEEECHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
ERLFNELATDILGTTEITYQEEAISLKAPFIRLSMSDAIAKYAENFDMNRINDREYLAEY
HHHHHHHHHHHHCCCCEEEHHHHHEEECCEEEEEHHHHHHHHHHCCCCCCCCHHHHHHHH
ASTTLKQQVKDVFGVGKLQTIIFEETAEHQLRQPTFITEYPAETSPLARRSDDNPEITDR
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCCCCCCCCCHHHH
FELFVGGRELANGFSELNDPEDQAERFLGQVAEKDAGDDEAMHFDAEYIEALSYGLPPTA
HHHEECCHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCHHEECCHHHHHHHHCCCCCCC
GEGIGIDRLVMLLTDSASIRDVILFPHMRRKLEG
CCCCCHHHHHHHHCCCCCHHHHHCCHHHHHHCCC
>Mature Secondary Structure 
SKPNNQNQQNNQEPAPEDANELIAQLQAKLDDIVASGKQPYPNTFKRTDYAQDLQAAFE
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHCHHHHHHHHHHH
GISKQEIADNDAKGEKTQVNVAGRVMLNRGAFIVIQDMTGRIQLYVARKELDEKTLADIK
CCCHHHHCCCCCCCCCEEEEEEEEEEEECCCEEEEECCCCCEEEEEEHHHHCHHHHHHHH
SLDLGDIVGVSGYIGRSGKGDLYVHIEEIELLTKALRPMPNKFHGLADVEARYRNRHLDL
CCCCHHHCCCCCCCCCCCCCCEEEEHHHHHHHHHHHCCCCHHHCCCHHHHHHHHCCCEEE
MTNETTRDTFMVRSQVISGIRKFMLNERFMEVETPMMHPIPGGAVARPFVTHHNALDMPL
EECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHCCCCCCCCE
YLRIAPELYLKRLVVGGFEKVFEINRSFRNEGVSTRHNPEFTMIEFYQAYADYHDLMDLT
EEEECHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
ERLFNELATDILGTTEITYQEEAISLKAPFIRLSMSDAIAKYAENFDMNRINDREYLAEY
HHHHHHHHHHHHCCCCEEEHHHHHEEECCEEEEEHHHHHHHHHHCCCCCCCCHHHHHHHH
ASTTLKQQVKDVFGVGKLQTIIFEETAEHQLRQPTFITEYPAETSPLARRSDDNPEITDR
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCCCCCCCCCHHHH
FELFVGGRELANGFSELNDPEDQAERFLGQVAEKDAGDDEAMHFDAEYIEALSYGLPPTA
HHHEECCHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCHHEECCHHHHHHHHCCCCCCC
GEGIGIDRLVMLLTDSASIRDVILFPHMRRKLEG
CCCCCHHHHHHHHCCCCCHHHHHCCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA