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The map label for this gene is purD [H]

Identifier: 93006227

GI number: 93006227

Start: 1720501

End: 1721829

Strand: Direct

Name: purD [H]

Synonym: Pcryo_1401

Alternate gene names: 93006227

Gene position: 1720501-1721829 (Clockwise)

Preceding gene: 93006225

Following gene: 93006228

Centisome position: 56.23

GC content: 47.18

Gene sequence:

>1329_bases
ATGATGAATATTTTGGTGATTGGTTCAGGTGGTCGTGAACACGCACTAGCATGGCAGTGCGCAAAAGATAATAATGTAAA
AAATATCTATATCGCGCCTGGTAATGCTGGCACCGCCCTTGAACCCAAATGCCAAAACGTCACTTTGGAAGACGCCACTG
ATGCAGGCGAGCACAGCGCTGTTATCAAGTTTTGTCAGAATAACGCTATCGACATGGTCATCGTTGGTCCAGAAGCACCG
TTGGTAACAGGCATTGTCGATGCTTGTCGTGCTGCTGGCATCAAAGCTTGGGGTCCAACAGCGTATTGCGCGCAGTTAGA
AGGCTCAAAGACTTTTGCCAAAGAATTCATGGAACAAAACAATATCCCAACGGCGGCTTATCAGGGCTTTACCGACGCAG
TAGCGGCAAAGGCTTATATCGATGGGCAAGGCGCACCTATTGTAATTAAAGCGGATGGTCTTGCTGCCGGTAAAGGGGTC
ATTGTTGCTGAGACGATCGAACAGGCGCATGAAGCCATTGACGATATGTTGGCAGATAATAAATTTGGCGATGCCGGTAG
CCGCGTGGTGATTGAGCAGTTTTTGCAAGGCGAGGAAGCCAGTTTTATCTGTATGGTCGATGGTGAAAATATCCTGCCAA
TGGCAACCAGCCAAGACCATAAGCGTGCTTTTGAAGGTGATGCGGGTCCAAACACAGGCGGCATGGGTGCATATTCTCCT
GCGCCAGTTGTCACTCAAGAGGTTCATGATAAAGTCATGGCACAAGTGATTCAGCCTGTTGTAGATGCGATGAAAGCTGC
CGGTTATCCTTATACCGGATTTTTATATGCTGGACTCATGATTGATGATGCGGGCGACCCGTATGTGATTGAGTTTAACT
GCCGTTTTGGCGATCCGGAGACGCAGCCTATTTTGATGCGTTTACAATCGTCAATGGTTGATTTGGTTGCACAAGGACTT
GAAGGTAAATTACCGAGCGAAGCCAACTGGGATGAGCGCCCTGCCCTTGGTATTGTCGTCGCCTCAAAAGGCTACCCTGA
AACTTCATCGAAAGGTGATGTGATTGCAGGCTTGCCAGAATTAAATAATGACAATCAAAATTCGGTAAAAGTTTTTCATG
CGGGCACGGCTTTTTCTCATGAAAATGCTCCTAGCAATGAGAAAGAAGTCGTGACCAATGGCGGGCGTGTATTATGTGTG
ACTGCATTAGCAGATAGCATCTCAGATGCACAGCAAGCAGCATTAACGGTCACTGCTGCCATTAGTTTTGATGGTGCTCA
TTATCGCCGTGATATCGGTCATCATGCTATTGCCCGTGAAAACATTTAA

Upstream 100 bases:

>100_bases
GCAGCATTTTTTTGTTAGAATAGCGCAGAATATTGGACAGTATTTTGCTGGCTCGCTATTAACTCACTTATTATGAATTT
TAATGCTAAGGGCAAGAGGT

Downstream 100 bases:

>100_bases
ATAGTAGTGATAAGTAGCACTTACCTATAGCGACTTTTGATAAATTCGACCTAGCGTGATGCTAGGTTGAATTTTTTGAT
TGGTTATAGTTTTTAAAGGC

Product: phosphoribosylamine--glycine ligase

Products: NA

Alternate protein names: GARS; Glycinamide ribonucleotide synthetase; Phosphoribosylglycinamide synthetase [H]

Number of amino acids: Translated: 442; Mature: 442

Protein sequence:

>442_residues
MMNILVIGSGGREHALAWQCAKDNNVKNIYIAPGNAGTALEPKCQNVTLEDATDAGEHSAVIKFCQNNAIDMVIVGPEAP
LVTGIVDACRAAGIKAWGPTAYCAQLEGSKTFAKEFMEQNNIPTAAYQGFTDAVAAKAYIDGQGAPIVIKADGLAAGKGV
IVAETIEQAHEAIDDMLADNKFGDAGSRVVIEQFLQGEEASFICMVDGENILPMATSQDHKRAFEGDAGPNTGGMGAYSP
APVVTQEVHDKVMAQVIQPVVDAMKAAGYPYTGFLYAGLMIDDAGDPYVIEFNCRFGDPETQPILMRLQSSMVDLVAQGL
EGKLPSEANWDERPALGIVVASKGYPETSSKGDVIAGLPELNNDNQNSVKVFHAGTAFSHENAPSNEKEVVTNGGRVLCV
TALADSISDAQQAALTVTAAISFDGAHYRRDIGHHAIARENI

Sequences:

>Translated_442_residues
MMNILVIGSGGREHALAWQCAKDNNVKNIYIAPGNAGTALEPKCQNVTLEDATDAGEHSAVIKFCQNNAIDMVIVGPEAP
LVTGIVDACRAAGIKAWGPTAYCAQLEGSKTFAKEFMEQNNIPTAAYQGFTDAVAAKAYIDGQGAPIVIKADGLAAGKGV
IVAETIEQAHEAIDDMLADNKFGDAGSRVVIEQFLQGEEASFICMVDGENILPMATSQDHKRAFEGDAGPNTGGMGAYSP
APVVTQEVHDKVMAQVIQPVVDAMKAAGYPYTGFLYAGLMIDDAGDPYVIEFNCRFGDPETQPILMRLQSSMVDLVAQGL
EGKLPSEANWDERPALGIVVASKGYPETSSKGDVIAGLPELNNDNQNSVKVFHAGTAFSHENAPSNEKEVVTNGGRVLCV
TALADSISDAQQAALTVTAAISFDGAHYRRDIGHHAIARENI
>Mature_442_residues
MMNILVIGSGGREHALAWQCAKDNNVKNIYIAPGNAGTALEPKCQNVTLEDATDAGEHSAVIKFCQNNAIDMVIVGPEAP
LVTGIVDACRAAGIKAWGPTAYCAQLEGSKTFAKEFMEQNNIPTAAYQGFTDAVAAKAYIDGQGAPIVIKADGLAAGKGV
IVAETIEQAHEAIDDMLADNKFGDAGSRVVIEQFLQGEEASFICMVDGENILPMATSQDHKRAFEGDAGPNTGGMGAYSP
APVVTQEVHDKVMAQVIQPVVDAMKAAGYPYTGFLYAGLMIDDAGDPYVIEFNCRFGDPETQPILMRLQSSMVDLVAQGL
EGKLPSEANWDERPALGIVVASKGYPETSSKGDVIAGLPELNNDNQNSVKVFHAGTAFSHENAPSNEKEVVTNGGRVLCV
TALADSISDAQQAALTVTAAISFDGAHYRRDIGHHAIARENI

Specific function: De novo purine biosynthesis; second step. [C]

COG id: COG0151

COG function: function code F; Phosphoribosylamine-glycine ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=439, Percent_Identity=48.74715261959, Blast_Score=400, Evalue=1e-112,
Organism=Homo sapiens, GI209869995, Length=439, Percent_Identity=48.74715261959, Blast_Score=400, Evalue=1e-112,
Organism=Homo sapiens, GI209869993, Length=439, Percent_Identity=48.74715261959, Blast_Score=400, Evalue=1e-112,
Organism=Homo sapiens, GI28416899, Length=439, Percent_Identity=48.74715261959, Blast_Score=400, Evalue=1e-111,
Organism=Escherichia coli, GI1790438, Length=441, Percent_Identity=58.7301587301587, Blast_Score=508, Evalue=1e-145,
Organism=Caenorhabditis elegans, GI17567511, Length=440, Percent_Identity=38.1818181818182, Blast_Score=287, Evalue=8e-78,
Organism=Saccharomyces cerevisiae, GI6321203, Length=450, Percent_Identity=39.7777777777778, Blast_Score=324, Evalue=2e-89,
Organism=Drosophila melanogaster, GI24582400, Length=440, Percent_Identity=45, Blast_Score=337, Evalue=1e-92,
Organism=Drosophila melanogaster, GI62471619, Length=440, Percent_Identity=44.5454545454545, Blast_Score=337, Evalue=1e-92,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR020561
- InterPro:   IPR000115
- InterPro:   IPR020560
- InterPro:   IPR020559
- InterPro:   IPR020562
- InterPro:   IPR011054 [H]

Pfam domain/function: PF01071 GARS_A; PF02843 GARS_C; PF02844 GARS_N [H]

EC number: =6.3.4.13 [H]

Molecular weight: Translated: 46685; Mature: 46685

Theoretical pI: Translated: 4.38; Mature: 4.38

Prosite motif: PS50975 ATP_GRASP ; PS00184 GARS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMNILVIGSGGREHALAWQCAKDNNVKNIYIAPGNAGTALEPKCQNVTLEDATDAGEHSA
CEEEEEECCCCCCCEEEEEECCCCCCCEEEEECCCCCCCCCCCCCCEEECCCCCCCCHHH
VIKFCQNNAIDMVIVGPEAPLVTGIVDACRAAGIKAWGPTAYCAQLEGSKTFAKEFMEQN
HHHHHCCCCEEEEEECCCCCHHHHHHHHHHHCCCCCCCCCEEEEECCCCHHHHHHHHHHC
NIPTAAYQGFTDAVAAKAYIDGQGAPIVIKADGLAAGKGVIVAETIEQAHEAIDDMLADN
CCCHHHHCCCHHHHHHEEEECCCCCEEEEEECCCCCCCCEEEHHHHHHHHHHHHHHHCCC
KFGDAGSRVVIEQFLQGEEASFICMVDGENILPMATSQDHKRAFEGDAGPNTGGMGAYSP
CCCCCCCHHHHHHHHCCCCCCEEEEECCCCEEECCCCCHHHHHCCCCCCCCCCCCCCCCC
APVVTQEVHDKVMAQVIQPVVDAMKAAGYPYTGFLYAGLMIDDAGDPYVIEFNCRFGDPE
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEHEEEEEEEEECCCCCEEEEEEEECCCCC
TQPILMRLQSSMVDLVAQGLEGKLPSEANWDERPALGIVVASKGYPETSSKGDVIAGLPE
CCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCEEECCCC
LNNDNQNSVKVFHAGTAFSHENAPSNEKEVVTNGGRVLCVTALADSISDAQQAALTVTAA
CCCCCCCCEEEEECCCCCCCCCCCCCCCCEEECCCCEEEEEHHHHHHHHHHHHEEEEEEE
ISFDGAHYRRDIGHHAIARENI
EEECCHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MMNILVIGSGGREHALAWQCAKDNNVKNIYIAPGNAGTALEPKCQNVTLEDATDAGEHSA
CEEEEEECCCCCCCEEEEEECCCCCCCEEEEECCCCCCCCCCCCCCEEECCCCCCCCHHH
VIKFCQNNAIDMVIVGPEAPLVTGIVDACRAAGIKAWGPTAYCAQLEGSKTFAKEFMEQN
HHHHHCCCCEEEEEECCCCCHHHHHHHHHHHCCCCCCCCCEEEEECCCCHHHHHHHHHHC
NIPTAAYQGFTDAVAAKAYIDGQGAPIVIKADGLAAGKGVIVAETIEQAHEAIDDMLADN
CCCHHHHCCCHHHHHHEEEECCCCCEEEEEECCCCCCCCEEEHHHHHHHHHHHHHHHCCC
KFGDAGSRVVIEQFLQGEEASFICMVDGENILPMATSQDHKRAFEGDAGPNTGGMGAYSP
CCCCCCCHHHHHHHHCCCCCCEEEEECCCCEEECCCCCHHHHHCCCCCCCCCCCCCCCCC
APVVTQEVHDKVMAQVIQPVVDAMKAAGYPYTGFLYAGLMIDDAGDPYVIEFNCRFGDPE
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEHEEEEEEEEECCCCCEEEEEEEECCCCC
TQPILMRLQSSMVDLVAQGLEGKLPSEANWDERPALGIVVASKGYPETSSKGDVIAGLPE
CCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCEEECCCC
LNNDNQNSVKVFHAGTAFSHENAPSNEKEVVTNGGRVLCVTALADSISDAQQAALTVTAA
CCCCCCCCEEEEECCCCCCCCCCCCCCCCEEECCCCEEEEEHHHHHHHHHHHHEEEEEEE
ISFDGAHYRRDIGHHAIARENI
EEECCHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12620739 [H]