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The map label for this gene is cysW [H]

Identifier: 93006214

GI number: 93006214

Start: 1697110

End: 1698042

Strand: Reverse

Name: cysW [H]

Synonym: Pcryo_1388

Alternate gene names: 93006214

Gene position: 1698042-1697110 (Counterclockwise)

Preceding gene: 93006215

Following gene: 93006213

Centisome position: 55.49

GC content: 45.34

Gene sequence:

>933_bases
ATGCAAATCTCTAATAGCTACGACTACCAAAGCAACCCAGCAACCAAAGATACGCCATGGGTACGCCGCACCTTTATTAT
CATTGCGGTTTTATTCATGGTTATTATGTTGGTAGTGCCGCTGCTAGCTGTGTTTTATGAAGCTTTTAAGGGTGGCTGGG
AATTATATATTGCCTCTCTCGTTGATCCTGAAGCCTTGCAGGCGATTAGGCTAACCTTGATTACTGCTGCGATTGTCTTA
CCGATTAATATGGTGATGGGTATTGCCATTGCGTGGCTAGTAACGCGTTATCATTTTAAAGGTAAGCAGTTGGTGACGAC
GTTGCTTGATTTACCATTCTCAGTGTCTCCCGTTGTAGCCGGTTTAATGTTTGTTCTATTATTTGGACTTAATTCTACGA
TTGGTGGCTGGCTTGAGAGCATGGGTTTTCAGGTAATTTATGCCGTACCCGGTATTATTTTAGCAACGTTATTTGTCACC
TTTCCTTTTGTGGCGCGTGAGTTAATACCGCTGATGCAAACGCAGGGCGATAGCGAAGAGCAGGCAGCATTAACGTTAGG
CGCGACGGGTTGGCAAACGTTTTGGCATGTGACACTGCCCAATATCAAATGGGCGCTGCTATATGGTTTGATTTTGACCA
ATGCACGGGCGATGGGGGAGTTTGGTGCGGTGAGTGTGGTCTCAGGCCATATCCGCGGAGAAACCAACACTATGCCGCTA
CTGGTTGAGATTGCCTATAATGACTATAACTTTACCGCCGCCTTTGCCTTATCGAGCTTACTTGCCGCACTTGCTTTGGT
CACGTTACTTATTCAGCAAATCATGACCAAACTACAAGATCACAAATTTGCCAAGTCTGAACGTTTGATAAGTGCGCCTG
AACTGCCAGTCAGTGCTAATGATACTGTTACAGAATCGGCAGGCAAATCATAA

Upstream 100 bases:

>100_bases
CGTCAGCCGTTGCATTATTTATGCTGCTTATCTCCTTTGTGATTTTATTGACCATTAATATCGTACAGTGGAAGCTGTCG
CGCCGTGTAGGAGCTCGCTA

Downstream 100 bases:

>100_bases
CGGTTCATAGAAGCCACTATTGCTATACGCCATACTATTGAAACTATCTGCTACGATAAAAGAAATAATAAGAGACACCA
TTATGAGCATCGAGATTCGT

Product: Sulfate ABC transporter, permease CysW

Products: ADP; phosphate; S2O32- [Cytoplasm]; SO42- [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 310; Mature: 310

Protein sequence:

>310_residues
MQISNSYDYQSNPATKDTPWVRRTFIIIAVLFMVIMLVVPLLAVFYEAFKGGWELYIASLVDPEALQAIRLTLITAAIVL
PINMVMGIAIAWLVTRYHFKGKQLVTTLLDLPFSVSPVVAGLMFVLLFGLNSTIGGWLESMGFQVIYAVPGIILATLFVT
FPFVARELIPLMQTQGDSEEQAALTLGATGWQTFWHVTLPNIKWALLYGLILTNARAMGEFGAVSVVSGHIRGETNTMPL
LVEIAYNDYNFTAAFALSSLLAALALVTLLIQQIMTKLQDHKFAKSERLISAPELPVSANDTVTESAGKS

Sequences:

>Translated_310_residues
MQISNSYDYQSNPATKDTPWVRRTFIIIAVLFMVIMLVVPLLAVFYEAFKGGWELYIASLVDPEALQAIRLTLITAAIVL
PINMVMGIAIAWLVTRYHFKGKQLVTTLLDLPFSVSPVVAGLMFVLLFGLNSTIGGWLESMGFQVIYAVPGIILATLFVT
FPFVARELIPLMQTQGDSEEQAALTLGATGWQTFWHVTLPNIKWALLYGLILTNARAMGEFGAVSVVSGHIRGETNTMPL
LVEIAYNDYNFTAAFALSSLLAALALVTLLIQQIMTKLQDHKFAKSERLISAPELPVSANDTVTESAGKS
>Mature_310_residues
MQISNSYDYQSNPATKDTPWVRRTFIIIAVLFMVIMLVVPLLAVFYEAFKGGWELYIASLVDPEALQAIRLTLITAAIVL
PINMVMGIAIAWLVTRYHFKGKQLVTTLLDLPFSVSPVVAGLMFVLLFGLNSTIGGWLESMGFQVIYAVPGIILATLFVT
FPFVARELIPLMQTQGDSEEQAALTLGATGWQTFWHVTLPNIKWALLYGLILTNARAMGEFGAVSVVSGHIRGETNTMPL
LVEIAYNDYNFTAAFALSSLLAALALVTLLIQQIMTKLQDHKFAKSERLISAPELPVSANDTVTESAGKS

Specific function: Part of the ABC transporter complex CysAWTP (TC 3.A.1.6.1) involved in sulfate/thiosulfate import. Probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG4208

COG function: function code P; ABC-type sulfate transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI87082099, Length=289, Percent_Identity=47.4048442906574, Blast_Score=278, Evalue=3e-76,
Organism=Escherichia coli, GI1788764, Length=209, Percent_Identity=37.3205741626794, Blast_Score=152, Evalue=4e-38,
Organism=Escherichia coli, GI1786980, Length=228, Percent_Identity=31.5789473684211, Blast_Score=91, Evalue=1e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515
- InterPro:   IPR011866
- InterPro:   IPR005667 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 34007; Mature: 34007

Theoretical pI: Translated: 5.65; Mature: 5.65

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQISNSYDYQSNPATKDTPWVRRTFIIIAVLFMVIMLVVPLLAVFYEAFKGGWELYIASL
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHEEHHHH
VDPEALQAIRLTLITAAIVLPINMVMGIAIAWLVTRYHFKGKQLVTTLLDLPFSVSPVVA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCHHHHHH
GLMFVLLFGLNSTIGGWLESMGFQVIYAVPGIILATLFVTFPFVARELIPLMQTQGDSEE
HHHHHHHHHCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCH
QAALTLGATGWQTFWHVTLPNIKWALLYGLILTNARAMGEFGAVSVVSGHIRGETNTMPL
HHEEEECCCCCHHEEEEECCCHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCCCCCCCEE
LVEIAYNDYNFTAAFALSSLLAALALVTLLIQQIMTKLQDHKFAKSERLISAPELPVSAN
EEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
DTVTESAGKS
CCCCCCCCCC
>Mature Secondary Structure
MQISNSYDYQSNPATKDTPWVRRTFIIIAVLFMVIMLVVPLLAVFYEAFKGGWELYIASL
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHEEHHHH
VDPEALQAIRLTLITAAIVLPINMVMGIAIAWLVTRYHFKGKQLVTTLLDLPFSVSPVVA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCHHHHHH
GLMFVLLFGLNSTIGGWLESMGFQVIYAVPGIILATLFVTFPFVARELIPLMQTQGDSEE
HHHHHHHHHCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCH
QAALTLGATGWQTFWHVTLPNIKWALLYGLILTNARAMGEFGAVSVVSGHIRGETNTMPL
HHEEEECCCCCHHEEEEECCCHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCCCCCCCEE
LVEIAYNDYNFTAAFALSSLLAALALVTLLIQQIMTKLQDHKFAKSERLISAPELPVSAN
EEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
DTVTESAGKS
CCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; S2O32- [Periplasm]; H2O; SO42- [Periplasm]; ATP [C]

Specific reaction: ATP + S2O32- [Periplasm] + H2O = ADP + phosphate + S2O32- [Cytoplasm] SO42- [Periplasm] + H2O + ATP = SO42- [Cytoplasm] + phosphate + ADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]