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The map label for this gene is prpB [H]

Identifier: 93006139

GI number: 93006139

Start: 1601355

End: 1602245

Strand: Reverse

Name: prpB [H]

Synonym: Pcryo_1311

Alternate gene names: 93006139

Gene position: 1602245-1601355 (Counterclockwise)

Preceding gene: 93006140

Following gene: 93006138

Centisome position: 52.36

GC content: 44.78

Gene sequence:

>891_bases
ATGACCCTATCAGCTGGCGCTCGCTTTCGCAGTGCAATGAAACAAAAAAATGATAATAATCAACCGCTACAGATTGCTGG
CGCAATTAACGCTTATACAGCAATGATGGCAACTCAAGTTGGTCATCAAGCCTTATATCTCTCTGGTGCTGGCGTGGCCA
ATGCCTCATTTGGCTTGCCAGATTTGGGTATGACTAGCCTTGATAACGTCTTAGAAGATGCGCGCCGTATTACCGATGCG
GTTGATACGCCATTATTGGTTGATATTGACACCGGTTTTGGTGGTGCATTTAATATTGCTCAAACCATTCGTAAAATGGA
AAAAGCCGGTGTGGCGGCGGTTCATATCGAAGACCAAGTGGCGCAAAAGCGTTGTGGTCATCGTCCAAATAAAGAAATTG
TTAGTATCTCAGAAATGGTTGATCGCCTAAAAGCGGCACTTGATGCCAAGACGGACAAAGATTTTGTGGTGATGGCACGT
ACCGACGCCCTATCTGTCGAAGGTCTTGATGCAGCGGTTGAGCGCGCGGTTGCTTTTCAAGAAGCTGGTGCTGATATGAT
TTTTGCCGAAGCACTAACGGATATTGAGATGTACCGTAAGTTTACCGATGTGCTTGATATTCCAGTCCTAGCTAATATGA
CCGAATTTGGTCAGACCGATCTATATACCACCGAGCAGTTATATGCGGTAGGTGTTGATATGGTGCTTTATCCATTATCA
GCCTTCCGTGCGATGAACAAAGCGGCATTAAACGTATATCAGCATCTGCTTGATGATGGCACGCAAGACAAAGTTGTCGA
CACCATGCAAACCCGTATGGAGCTGTATGACTTCTTAAACTATCATGAGTTTGAACAAACGCTAGACAAACTGTTTGCTG
ATAATAAGTAG

Upstream 100 bases:

>100_bases
AATCCCGCTTTGCAGTCCTGAATAATTGGTTATAATCAAGCACAACGATTGAATCGCGAATGATAACGACAAGATCTTCC
CTCCACGTAAGGACCACGAC

Downstream 100 bases:

>100_bases
TCTTAGCGGTTAAAAGCGTTGGTTTTCCTATCAATGAGGATGTCAACGCAGTATTTGATATTTGCAGCATCTTTATCTTT
TATCCAACACAAGCTACCAA

Product: 2-methylisocitrate lyase

Products: NA

Alternate protein names: 2-methylisocitrate lyase [H]

Number of amino acids: Translated: 296; Mature: 295

Protein sequence:

>296_residues
MTLSAGARFRSAMKQKNDNNQPLQIAGAINAYTAMMATQVGHQALYLSGAGVANASFGLPDLGMTSLDNVLEDARRITDA
VDTPLLVDIDTGFGGAFNIAQTIRKMEKAGVAAVHIEDQVAQKRCGHRPNKEIVSISEMVDRLKAALDAKTDKDFVVMAR
TDALSVEGLDAAVERAVAFQEAGADMIFAEALTDIEMYRKFTDVLDIPVLANMTEFGQTDLYTTEQLYAVGVDMVLYPLS
AFRAMNKAALNVYQHLLDDGTQDKVVDTMQTRMELYDFLNYHEFEQTLDKLFADNK

Sequences:

>Translated_296_residues
MTLSAGARFRSAMKQKNDNNQPLQIAGAINAYTAMMATQVGHQALYLSGAGVANASFGLPDLGMTSLDNVLEDARRITDA
VDTPLLVDIDTGFGGAFNIAQTIRKMEKAGVAAVHIEDQVAQKRCGHRPNKEIVSISEMVDRLKAALDAKTDKDFVVMAR
TDALSVEGLDAAVERAVAFQEAGADMIFAEALTDIEMYRKFTDVLDIPVLANMTEFGQTDLYTTEQLYAVGVDMVLYPLS
AFRAMNKAALNVYQHLLDDGTQDKVVDTMQTRMELYDFLNYHEFEQTLDKLFADNK
>Mature_295_residues
TLSAGARFRSAMKQKNDNNQPLQIAGAINAYTAMMATQVGHQALYLSGAGVANASFGLPDLGMTSLDNVLEDARRITDAV
DTPLLVDIDTGFGGAFNIAQTIRKMEKAGVAAVHIEDQVAQKRCGHRPNKEIVSISEMVDRLKAALDAKTDKDFVVMART
DALSVEGLDAAVERAVAFQEAGADMIFAEALTDIEMYRKFTDVLDIPVLANMTEFGQTDLYTTEQLYAVGVDMVLYPLSA
FRAMNKAALNVYQHLLDDGTQDKVVDTMQTRMELYDFLNYHEFEQTLDKLFADNK

Specific function: Catalyzes the formation of pyruvate and succinate from 2-methylisocitrate [H]

COG id: COG2513

COG function: function code G; PEP phosphonomutase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate lyase/PEP mutase superfamily. Methylisocitrate lyase family [H]

Homologues:

Organism=Escherichia coli, GI1786525, Length=294, Percent_Identity=60.8843537414966, Blast_Score=375, Evalue=1e-105,
Organism=Escherichia coli, GI1790445, Length=237, Percent_Identity=28.2700421940928, Blast_Score=75, Evalue=8e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000918
- InterPro:   IPR018523
- InterPro:   IPR012695
- InterPro:   IPR015813 [H]

Pfam domain/function: PF00463 ICL [H]

EC number: =4.1.3.30 [H]

Molecular weight: Translated: 32554; Mature: 32423

Theoretical pI: Translated: 4.44; Mature: 4.44

Prosite motif: PS00161 ISOCITRATE_LYASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
5.1 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
4.7 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLSAGARFRSAMKQKNDNNQPLQIAGAINAYTAMMATQVGHQALYLSGAGVANASFGLP
CCCCCHHHHHHHHHHCCCCCCCEEEEHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCC
DLGMTSLDNVLEDARRITDAVDTPLLVDIDTGFGGAFNIAQTIRKMEKAGVAAVHIEDQV
CCCHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHCCEEEEEEHHHH
AQKRCGHRPNKEIVSISEMVDRLKAALDAKTDKDFVVMARTDALSVEGLDAAVERAVAFQ
HHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHH
EAGADMIFAEALTDIEMYRKFTDVLDIPVLANMTEFGQTDLYTTEQLYAVGVDMVLYPLS
HCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHCHHHHCCCCCCHHHHHHHHHHHHHHHHHH
AFRAMNKAALNVYQHLLDDGTQDKVVDTMQTRMELYDFLNYHEFEQTLDKLFADNK
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TLSAGARFRSAMKQKNDNNQPLQIAGAINAYTAMMATQVGHQALYLSGAGVANASFGLP
CCCCHHHHHHHHHHCCCCCCCEEEEHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCC
DLGMTSLDNVLEDARRITDAVDTPLLVDIDTGFGGAFNIAQTIRKMEKAGVAAVHIEDQV
CCCHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHCCEEEEEEHHHH
AQKRCGHRPNKEIVSISEMVDRLKAALDAKTDKDFVVMARTDALSVEGLDAAVERAVAFQ
HHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHH
EAGADMIFAEALTDIEMYRKFTDVLDIPVLANMTEFGQTDLYTTEQLYAVGVDMVLYPLS
HCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHCHHHHCCCCCCHHHHHHHHHHHHHHHHHH
AFRAMNKAALNVYQHLLDDGTQDKVVDTMQTRMELYDFLNYHEFEQTLDKLFADNK
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]